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Figure 11 from: Mikó I, Masner L, Ulmer JM, Raymond M, Hobbie J, Tarasov S, Margaría CB, Seltmann KC, Talamas EJ (2021) A semantically enriched taxonomic revision of Gryonoides Dodd, 1920 (Hymenoptera, Scelionidae), with a review of the hosts of Teleasinae. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 523-573. https://doi.org/10.3897/jhr.87.72931

Figure 11 Gryonoides glabriceps Dodd, 1920, female (CNCHymen_132135) A head, mesosoma and metasoma, anterolateral view B head and antenna, ventrolateral view.

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Figures 1-4 from: Mikó I, Raymond M, Talamas EJ (2021) New family-level characters for Platygastroidea. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 235-249. https://doi.org/10.3897/jhr.87.72906

Figures 1-4 Mesopectus, ventrolateral view 1Amitus (USNMENT00989622_2) 2Fidiobia (OSUC 698059) 3Iphitrachelus (USNMENT01029164) 4Sceliotrachelinae sp. (OSUC 698061).

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Supplementary material 5 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

BOLD BINs

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Figure 24 from: Mikó I, Raymond M, Talamas EJ (2021) New family-level characters for Platygastroidea. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 235-249. https://doi.org/10.3897/jhr.87.72906

Figure 24 Modified phylogeny from Chen et al. (2021) with characters mapped to the topology. Parenthetical numerals indicate the corresponding character: state from Table 3A mesofurcal bridge present (13:3) B mesepimeral sulcus present (13:1) C first flexor of the fore wing with pronotal site of origin (2:1) D first flexor of the hind wing with mesopectal site of origin (15:1) E discrimenal lamella not extending anteriorly to acetabulum (6:1) F mesofurcal bridge absent (11:0) G first flexor of the fore wing with mesopectal site of origin (2:0) H mesepimeral sulcus absent (13:0) I first flexor of the hind wing with metapectal site of origin (15:0) J anterior mesofurcal pit present (7:1) K discrimenal lamella extending to acetabulum (6:0) L anterior mesofurcal pit absent (7:0).

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Figure 22 from: Mikó I, Masner L, Ulmer JM, Raymond M, Hobbie J, Tarasov S, Margaría CB, Seltmann KC, Talamas EJ (2021) A semantically enriched taxonomic revision of Gryonoides Dodd, 1920 (Hymenoptera, Scelionidae), with a review of the hosts of Teleasinae. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 523-573. https://doi.org/10.3897/jhr.87.72931

Figure 22 Gryonoides mirabilicornis Masner & Mikó, sp. nov. A A4 and A5 showing elongate release and spread structures (rss) B release and spread structures (tyloids) correspond to class 3 gland cells (Noirot and Quennedey 1976) and are characterised by the presence of cuticular canals (arrows). Release and spread structures are present only on the fifth flagellomere in most Platygastroidea (except Sparasionini).

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Supplementary material 2 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Sequence data

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Supplementary material 3 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Phylogenetic tree

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Figure 14 from: Mikó I, Masner L, Ulmer JM, Raymond M, Hobbie J, Tarasov S, Margaría CB, Seltmann KC, Talamas EJ (2021) A semantically enriched taxonomic revision of Gryonoides Dodd, 1920 (Hymenoptera, Scelionidae), with a review of the hosts of Teleasinae. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 523-573. https://doi.org/10.3897/jhr.87.72931

Figure 14 Gryonoides mexicali Masner & Mikó, sp. nov. A habitus of female, dorsal view (CNCHymen_132006) B head and proximal antenna of male anteroventral view C habitus of female, lateral view (CNCHymen_132006) D head, anterior view (CNCHymen_132026).

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Figure 25 from: Mikó I, Masner L, Ulmer JM, Raymond M, Hobbie J, Tarasov S, Margaría CB, Seltmann KC, Talamas EJ (2021) A semantically enriched taxonomic revision of Gryonoides Dodd, 1920 (Hymenoptera, Scelionidae), with a review of the hosts of Teleasinae. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 523-573. https://doi.org/10.3897/jhr.87.72931

Figure 25 Distribution of Gryonoides fuscoclavatus Masner & Mikó, sp. nov. and Gryonoides flaviclavatus Masner & Mikó, sp. nov.

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Supplementary material 4 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Table

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Figures 7-10 from: Mikó I, Raymond M, Talamas EJ (2021) New family-level characters for Platygastroidea. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 235-249. https://doi.org/10.3897/jhr.87.72906

Figures 7-10 Mesopectus, ventral view 7Orwellium enigmaticum (OSUC 226542) 8Janzenella innupta (OSUC 264384) 9Archaeoteleia gracilis (OSUC 163002) 10Sceliomorpha (USNMENT00989930).

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Figures 14-17 from: Mikó I, Raymond M, Talamas EJ (2021) New family-level characters for Platygastroidea. In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 235-249. https://doi.org/10.3897/jhr.87.72906

Figures 14-17 14Trichacis sp. (FSCA 00000276), ventral view 15Amitus (UNHC_1046725A) interior of head and mesosoma, lateral view 16Isocybus (OSUC 334137), mesopectus, ventral view 17Isocybus (unvouchered), interior of mesopectus, lateral view.

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Figures 22-23 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Figures 22-23 Gryon misellum22 lectotype male (NMINH_2018_11_02), head, anterolateral view 23 paralectotype female (NMINH_2018_11_03), head, mesosoma, metasoma, dorsolateral view.

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Supplementary material 1 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

GenBank accession number

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Figures 15-20 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Figures 15-20 Metasoma 15Gryon aetherium (FSCA 00094873), dorsolateral view 16Gryon aetherium (FSCA 00094873) ventrolateral view 17Hadronotus bicolor (FSCA 00091193), dorsolateral view 18Hadronotus bicolor (FSCA 00091193), lateral view 19Hadronotus carinatifrons (USNMENT01335649), dorsolateral view 20Hadronotus carinatifrons, ventrolateral view.

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Figures 9-14 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Figures 9-14 Mesosoma, lateral view 9Gryon aetherium (FSCA 00094874) 10Hadronotus hogenakalensis (DPI_FSCA 00008722) 11Hadronotus ater (FSCA 00094730) 12Hadronotus pennsylvanicus (FSCA 00091081) 13Hadronotus anserculus, holotype female 14Hadronotus canus, holotype female.

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Figures 5-8 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Figures 5-8 Scutellar-axillar complex, lateral view 5Gryon aetherium (USNMENT01109155) 6Duta (USNMENT01109621_2) 7Hadronotus hogenakalensis (DPI_FSCA 00008722) 8Hadronotus carinatifrons (USNMENT01335649).

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Figure 4 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Figure 4 Phylogenetic relationships of Scelionidae based on a maximum likelihood analysis of 479 COI sequences. Branches in blue and red indicate Hadronotus and Gryon, respectively. Terminals belonging to G. aetherium are shown to the right of the phylogenetic tree. Terminals highlighted in yellow correspond to adventive G. aetherium specimens collected in Mexico and California. Scale bars indicate the expected number of substitutions per site.

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Figures 113-116 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Figures 113-116 Gryon brevipenne (OSUC 395663) 113 mesosoma and metasoma, dorsolateral view 114 mouthparts, anteroventral view 115 hind tibia, posterior view 116 antennal clava, ventrolateral view.

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Figure 3 from: Talamas EJ, Bremer JS, Moore MR, Bon M-C, Lahey Z, Roberts CG, Combee LA, McGathey N, van Noort S, Timokhov AV, Hougardy E, Hogg B (2021) A maximalist approach to the systematics of a biological control agent: Gryon aetherium Talamas, sp. nov. (Hymenoptera, Scelionidae). In: Lahey Z, Talamas E (Eds) Advances in the Systematics of Platygastroidea III. Journal of Hymenoptera Research 87: 323-480. https://doi.org/10.3897/jhr.87.72842

Figure 3 Position and phylogenetic relationships of Gryon relative to other Scelionidae based on the topology depicted in Figure 1. Colored boxes above branches correspond to the level of support obtained for that branch based on the support metric. Branches annotated with a single box received equal levels of support in all analyses. The scale bar indicates the expected number of substitutions per site.

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