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FIGURE 18. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 18. Maximum parsimony 16S rRNA phylogram for species in the Boophis luteus group. From 470 total characters, 347 were constant and 111 parsimony informative. MP searches retained 60 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. The species newly described herein is in bold.

FIGURE 12 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 12. Male holotype of Boophis roseipalmatus sp. nov. (ZSM 211/2004) from Montagne d'Ambre National Park: (A) dorsolateral view; (B) ventral view.

FIGURE 3 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 3. Spectrograms and waveforms of calls of Boophis piperatus sp. nov. from near Vohiparara, Ranomafana National Park (recorded on 28 January 2004, air temperature 20–21°C): (A) call type 1, (B) call type 2 (see text).

FIGURE 16. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 16. Maximum parsimony 16S rRNA phylogram for species in the Boophis albilabris group. From 535 total characters, 445 were constant and 35 parsimony informative. MP searches retained 6 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. The species newly described herein is in bold.

FIGURE 4 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 4. Dorsolateral view (A) and ventral view (B) of male of Boophis andrangoloaka (FGZC 2139) from Ambohitantely; (C) dorsolateral view of Boophis andrangoloaka (ZFMK 60134) from Ambohitantely; dorsolateral view (D) and ventral view (E) of a dark coloured specimen of Boophis rhodoscelis from Ranomafanakely; (F) dorsolateral view of a light coloured specimen of Boophis rhodoscelis from Ranomafanakely.

FIGURE 5 in Molecular phylogenetics and taxonomy of leaf-toed geckos (Phyllodactylidae: Phyllodactylus) inhabiting the peninsula of Baja California

FIGURE 5. Phylogenetic relationships of Mexican Phyllodactylus based on maximum parsimony (MP; A) and Bayesian inference (BI; B) of mitochondrial DNA (mtDNA). Numbers above nodes on MP tree represent nonparametric bootstrap proportions (BSP). Numbers above nodes on the Bayesian topology represent Bayesian posterior probabilities (BPP; * = 1.0). Gekko gecko was removed from the BI tree due to its long branch length. Refer to text for a description of loci and total base pairs (bp) sequenced.

FIGURE 2 in Molecular phylogenetics and taxonomy of leaf-toed geckos (Phyllodactylidae: Phyllodactylus) inhabiting the peninsula of Baja California

FIGURE 2. Phylogenetic relationships of Mexican Phyllodactylus based on a branch-and-bound maximum parsimony (MP) analysis of the concatenated dataset. Numbers above nodes represent MP bootstrap proportions (BSP) resulting from 10,000 pseudoreplicates.

FIGURE 3 in Molecular phylogenetics and taxonomy of leaf-toed geckos (Phyllodactylidae: Phyllodactylus) inhabiting the peninsula of Baja California

FIGURE 3. Phylogenetic relationships of Mexican Phyllodactylus based on Bayesian inference (BI) of the concatenated dataset. Numbers above and adjacent to nodes represent Bayesian posterior probabilities (BPP; * = 1.0) sampled from the posterior distribution of trees. Gekko gecko was removed from the tree due to its long branch length.

FIGURE 4 in Molecular phylogenetics and taxonomy of leaf-toed geckos (Phyllodactylidae: Phyllodactylus) inhabiting the peninsula of Baja California

FIGURE 4. Phylogenetic relationships of Mexican Phyllodactylus based on maximum parsimony (MP; A) and Bayesian inference (BI; B) of nuclear DNA (nDNA). Numbers above nodes on MP tree represent nonparametric bootstrap proportions (BSP). Numbers above nodes on the Bayesian topology represent Bayesian posterior probabilities (BPP; * = 1.0). Refer to text for a description of loci and total base pairs (bp) sequenced.

FIGURE 1 in Molecular phylogenetics and taxonomy of leaf-toed geckos (Phyllodactylidae: Phyllodactylus) inhabiting the peninsula of Baja California

FIGURE 1. Map of Baja California, the Gulf of California, southwestern USA, and northwestern Mexico illustrating the geographic location of samples of Phyllodactylus included in this study. Sample numbers correspond to those presented in Table 1. The break in geographic ranges in the Isthmus of La Paz region is due to unsuitable natural habitat (lack of rocky outcrops). Gray area represents the geographic range of P. nocticolus, black area represents the geographic range of P. xanti, striped area represents the range of P. unctus.

Figure 9. The 50 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 9. The 50% majority rule consensus trees from Bayesian analysis generated from the 18S rRNA gene data set with a transitional model of invariable sites and a gamma-shaped distribution model. Posterior probabilities more than 65% are given for appropriate clades; bootstrap values greater than 50% are given on appropriate clades in the maximum likelihood analysis. Newly obtained sequences are in bold letters.

Figure 7. The 50 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 7. The 50% majority rule consensus trees from Bayesian analysis generated from the D2–D3 of 28S rRNA gene data set with a general time reversible of invariable sites and a gamma-shaped distribution model. Posterior probabilities more than 65% are given for appropriate clades; bootstrap values greater than 50% are given on appropriate clades in the maximum likelihood analysis. Newly obtained sequences are in bold letters.

Figure 6 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 6. Scanning electron microscope photographs of Tylenchorhynchus mediterraneus sp. nov. A, female anteri- or region; B, C, en face view showing oral (oa) and amphidial (am) apertures; D, lateral fields at mid-body; E, female tail showing anus (a). Scale bars: A = 20 μm; B, C, D = 10 μm; E = 20 μm.

Figure 4 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 4. Line drawings of Tylenchorhynchus mediterraneus sp. nov. A, female pharyngeal region; B, vulval region showing part of gonads and spermatheca; C, female lip region; D, details of lip region showing oral disc (en face view); E, F, male tails showing spicules and gubernaculum; G–I, female tails, showing areolated lateral fields and phasmid in the middle.

Figure 8. The 50 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 8. The 50% majority rule consensus trees from Bayesian analysis generated from the ITS rRNA gene data set with a transversional model of invariable sites and a gamma-shaped distribution model. Posterior probabilities more than 65% are given for appropriate clades; bootstrap values greater than 50% are given on appropriate clades in the maximum likelihood analysis. Newly obtained sequences are in bold letters.

Figure 3 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 3. Scanning electron microscope photographs of Bitylenchus hispaniensis sp. nov. A, female lip region; B, en face view showing oral (oa) and amphidial (am) apertures; C, lateral fields at mid-body; D, E, female tails showing anus (a) and phasmid (ph); F, male tail showing spicules (sp). Scale bars: A, C = 10 μm; B = 5 μm; D–F = 20 μm.

Figure 2 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 2. Photomicrographs of Bitylenchus hispaniensis sp. nov. A, whole body of female and male; B, female pharyngeal region; C, female lip region; D, vulval region; E, female tails; F, male tail; G, lateral fields at mid-body. Scale bars: A = 50 μm, B, E = 10 μm; C, D, G = 5 μm; F = 20 μm.

Figure 5 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 5. Photomicrographs of Tylenchorhynchus mediterraneus sp. nov. A, whole body of female and male; B, female pharyngeal region showing excretory pore (ep); C, female lip region showing stylet knobs (kn); D, vulval region; E–G, female tails showing anus (a) and phasmid (ph); H, male tail showing spicules (sp) and gubernaculum (gb). Scale bars: A = 50 μm, B, D = 20 μm; C, E–H = 10 μm.

Figure 1 in Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny

Figure 1. Line drawings of Bitylenchus hispaniensis sp. nov. A, female pharyngeal region; B, vulval region showing part of gonads and spermatheca; C, female lip region; D, details of lip region showing oral disc (en face view); E, F, male tails showing spicules and gubernaculum; G–J, female tails, with H and I showing intestinal fasciculi/sinuous canals present and extending back to tail, and J with areolated lateral fields and phasmid in the middle.

FIGURE 24 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 24. Spectrogram and waveform of advertisement calls of: (A) Boophis miadana sp. nov. from Andohahela National Park, 1550 m a.s.l. (recorded on 26 January 2005, air temperature app. 17.6°C); (B) Boophis haingana sp. nov. from Andohahela National Park, low altitude (recorded on 3 February 2004, air temperature app. 23.2°C).