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Effects of microarthropod exclusion and water amendments on fluffgrass growth at the Jornada Basin LTER, March to September 1986
This data package contains fluff grass (Dasyochloa pulchella) growth measurements taken during the growing period in 1986 (March, July, and September). The purpose of this study was to test how watering, and changing densities of soil microarthropods and nematodes, results in changes in fluff grass growth. Twenty 6 x 6 m plots were established with a 3 m buffer between plots. Five plots were randomly assigned to one of four treatments: 1) Chlordane to exclude microarthropods, 2) Chlordane and water, 3) Water, and 4) Control. Within each plot, fluff grass plants were permanently tagged for continuous measurements of plant diameter and height during the three sampling dates. Data consists of the date of measurement, the longest diameter (cm), the diameter (cm) perpendicular to the longest diameter, and height (cm). This study was completed in September 1986.
Effects of microarthropod exclusion and water amendments on fluffgrass rhizosphere nitrate (NO3) at the Jornada Basin LTER, 1986-1987
This data package contains data on nitrogen availability (as nitrate, NO3-), measured with ion exchange resin bags, in fluffgrass (Dasyochloa pulchella) rhizosphere soil from 1986-1987. This study was conducted at the Chihuahuan Desert Rangeland Research Center to test how watering, and changing densities of soil microarthropods and nematodes, impacts fluffgrass growth and nutrient cycling. An ion exchange resin bag technique (Binkley 1984, Lajtha 1988) was used to determine nitrate availability. Twenty 6 x 6 m plots were established with a 3 m buffer between plots. Five plots were randomly assigned to one of four treatments:1) Chlordane (to exclude microarthropods), 2) Chlordane and water, 3) Water, and 4) Control. Ten anion exchange bags were placed in the rhizosphere of a fluffgrass plant in each plot. Bags were left in the field 3 months, collected, brought to the lab and analyzed for available nitrate (NO3-) using an automated cadmium reduction procedure. The data table contains bag collection date, treatment, and NO3- (mg/kg) concentration. This study is complete.
Effects of microarthropod exclusion and water amendments on fluffgrass rhizosphere ammonium (NH4) at the Jornada Basin LTER, 1986-1987
This data package contains data on nitrogen availability (as ammonium, NH4+), measured with ion exchange resin bags, in fluffgrass (Dasyochloa pulchella) rhizosphere soil from 1986-1987. This study was conducted at the Chihuahuan Desert Rangeland Research Center to test how watering, and changing densities of soil microarthropods and nematodes, impacts fluffgrass growth and nutrient cycling. An ion exchange resin bag technique (Binkley 1984, Lajtha 1988) was used to determine ammonium availability. Twenty 6 x 6 m plots were established with a 3 m buffer between plots. Five plots were randomly assigned to one of four treatments:1) Chlordane (to exclude microarthropods), 2) Chlordane and water, 3) Water, and 4) Control. Two cation exchange bags were placed in the rhizosphere of a fluffgrass plant in each plot. Bags were left in the field 3 months, collected, brought to the lab and analyzed for available ammonium (NH4+) using a salicylate procedure. The data table contains bag collection date, treatment, and NH4+ (mg/kg) concentration. This study is complete.
Effects of microarthropod exclusion and water amendments on fluffgrass growth, root nitrogen, and mite and nematode abundance at the Jornada Basin LTER site, 1986-1987
This data package contains data on microarthropods, nematodes, fluff-grass (Dasyochloa pulchella) growth, and root nitrogen in samples from study plots sampled approximately monthly between May 1986 and August 1987 at the Jornada Basin LTER site in southern New Mexico USA. The purpose of this study was to test how watering, and changing densities of soil microarthropods and nematodes, results in changes in fluff grass growth and root nitrogen. Twenty 6 x 6 m plots were established with a 3 m buffer between plots. Five plots were randomly assigned to one of four treatments: 1) Chlordane to exclude microarthropods, 2) Chlordane and water, 3) Water, and 4) Control. At monthly intervals, randomly selected fluff grass plants were collected from each plot. This data set consists of plant diameters (cm), mite soil weight (g), root weight (g), nematode soil weight (g), root total nitrogen (mg/g), and nematode number. This study was completed in November 1987.
Effects of microarthropod exclusion and water amendments on fluffgrass rhizosphere total soil nitrogen at the Jornada Basin, 1986-1987
This data package contains data on soil nitrogen content in the rhizosphere of fluff grass (Dasyochloa pulchella) plants collected from 1986-1987 at the Jornada Basin LTER site. The purpose of this study was to test how watering, and changing densities of soil microarthropods and nematodes, results in changes in fluff grass rhizosphere total nitrogen. Twenty 6 x 6 m plots were established with a 3 m buffer between plots. Five plots were randomly assigned to one of four treatments: 1) Chlordane to exclude microarthropods, 2) Chlordane and water, 3) Water, and 4) Control. Every three months from June 1986 to January 1987, randomly selected fluff grass plants were harvested from each plot and rhizosphere soil samples were collected for total nitrogen digestion analysis. This data set consists of sampling date, treatment, subsample number, and total nitrogen (mg/kg). This study was completed in January 1987.
Drought and herbivory effects on woody plant seedling establishment and grass competition in the Jornada Basin, 2016-2018
This dataset contains observations of seedling establishment and grass competition under precipitation manipulations, and herbivory and granivory exclosure treatments, in the Chihuahuan desert of southern New Mexico, USA. The experiment took place at the Jornada Basin LTER site. We used a rainfall manipulation system and various herbivore exclosures in a factorial design, to test hypotheses about how precipitation (PPT), competition between grasses and shrub seedlings, and predation affect the germination and first-year survival of Mesquite (Prosopis glandulosa), a shrub that has encroached in Southern Great Plains and Chihuahuan Desert grasslands. Data collected in these files include seedling counts in each treatment over observation years 2016 to 2018. This data supports the related publication in Ecological Applications (Weber-Grullon et al. in press). The dataset is complete.
PSC01 Exploring effects on stream ecosystem properties by two size classes of prairie stream cyprinids
Losses in freshwater fish diversity might produce a loss in important ecological services provided by fishes in particular habitats. An important gap in our understanding of ecosystem services by fishes is the influence of individuals from different size classes, which is predicted based on known ontogenetic shifts in habitat and diet. I used twenty experimental stream mesocosms located on Konza Prairie Biological Station (KPBS), KS, USA to assess the influence of fish size on ecosystem properties. Mesocosms included two macrohabitats: one riffle upstream from one pool filled with consistent pebble and gravel substrate. There were four experimental and one control treatment, each replicated four times (N = 20). I used two size classes of Central Stonerollers (Campostoma anomalum) and Southern Redbelly Dace (Chrosomus erythrogaster). Five ecosystem properties were assessed: algal filament length (cm), benthic chlorophyll a (µg/cm2), benthic organic matter (g/m2), macroinvertebrate biomass (g/m2), and stream metabolism (g O2/m2/day1). Size structure of fish populations affected some, but not all, ecosystem properties and these effects were dependent upon species identity. Size structure of both species had effects on algal filament lengths where stonerollers of both size classes reduced algal filaments, but only small redbelly dace kept filaments short. A better understanding of the relationship between these prairie stream minnows and their small stream habitats could be useful to both predict changes in stream properties if species are lost or size structure shifts, and to redbelly dace populations, a Species In Need of Conservation.
SRM01 ShRaMPs (Shrub Rainout Manipulation Plots): interactive effects of drought and fire on grass and shrub physiology and productivity at Konza Prairie
ShRaMPs (Shrub Rainout Manipulation Plots) is a drought x fire experiment aimed at understanding the interactive effects of drought and fire frequency on tallgrass prairie communities experiencing varying degrees of shrub encroachment. Passive rainout shelters were constructed over existing, mature shrub islands and co-existing herbaceous communities on neighboring 1-year and 4-year burn watersheds (K1B and K4A). Shelters were either 'control' (ambient precipitation) or 'drought' (~50% precipitation reduction). A variety of leaf-level (gas exchange, water potential, turgor loss point, leaf δ13C) and whole-plant level (herbaceous and shrub biomass, plant water uptake) variables were measured during the 2019-2022 growing seasons to determine the physiological responses of a dominant C4 grass (Andropogon gerardii; big bluestem) and an encroaching clonal shrub (Cornus drummondii; rough-leaf dogwood). Soil volumetric water content was measured on-site for the duration of the experiment.
SNE01 Species richness, community evenness (Evar) and ANPP effects of nitrogen addition across a gradient of 8 levels in a semi-arid shortgrass steppe and a mesic tallgrass prairie, 2014-2018
This dataset contains the first five years (2014-2018) of the effect of nitrogen addition on species richness, species evenness (Evar) and productivity for a long-term nitrogen addition gradient experiment in two North American grasslands: a semi-arid shortgrass steppe and a mesic tallgrass prairie. Fertilization with time-release urea has been on-going since 2014 in a gradient of eight levels: 0, 2.5, 5, 10, 15, 20, 30 g/m-2. The effect of nitrogen on richness, evenness and Aboveground Net Primary Productivity (ANPP g/m-2 yr) is calculated as the absolute change in value from control plots to treatment plots within each block.
MCR LTER: Coral Reef: Effects of Flow and Temperature on Growth and Photophysiology of Scleractinian Corals in Moorea, French Polynesia
To test for threshold effects in the response of coral physiology to increasing seawater flow, field and laboratory experiments were conducted in Moorea. First, the growth of juvenile massive Porites spp. and branching P. irregularis was compared among habitats differing in water motion. Growth of massive Porites spp. responded to flow in a pattern consistent with a threshold effect, whereas growth of P. irregularis increased linearly with flow. Second, a recirculating flume was used to test the effect of flow on photophysiology (ΔF⁄Fm'), effective photochemical efficiency) for massive Porites spp.; ΔF⁄Fm' displayed a threshold response at 23 cm/sec and 28 °C, but not at 31 °C. Finally, intra-colony variation in the response of ΔF⁄Fm' to flow and temperature was explored to evaluate the functional significance of colony shape in small corals. ΔF⁄Fm' on the top and upstream surfaces of massive Porites spp. responded with a threshold effect of flow at 28 °C (but not 31 °C), but ΔF⁄Fm' on downstream surfaces was unresponsive to flow. ΔF⁄Fm' for P. irregularis was less responsive to flow than for massive Porites spp., suggesting that the photophysiological response of corals to varying flow speeds may differ between species and morphologies. Together, these results emphasize that flow can have diverse effects on the physiology of corals, with the outcome depending on flow speed, temperature, location on the colony, and perhaps morphology. These data were published in The Biological Bulletin and were part of the masters thesis of W. Goldenheim (2011).
Effect of varying post field collection filtration times on lake water nutrient analysis for Green Lake 3 and Green Lake 4, 2025.
After field collection, filtration time on lake and stream samples can vary. To test how this affects nutrient measurements we filtered samples from Green Lakes 3 and 4 at the time of collection in the field, immediately upon returning to the lab, 24 hours, and 48 hours after collection. Samples were then frozen and analyzed for chloride, nitrate and sulfate. Chloride and nitrate were below detection limits so only sulfate is reported. There was no statistically significant loss of sulfate as time progressed, indicating current filtration methods (<48 hours after collection) are acceptable for samples being analyzed via ion chromatography.
Biogeochemical rate data and sediment properties of samples used for a controlled flow through experiment testing the effect of nitrate on organic matter decomposition, PIE LTER, Plum Island Sound estuary, Massachusetts.
In this dataset, we used a controlled flow-through reactor (FTR) experiment to test the role of nitrate as an electron acceptor, and its effect on organic matter decomposition and the associated microbial community in salt marsh sediments. Organic matter decomposition significantly increased in response to nitrate, even at sediment depths typically considered resistant to decomposition. The use of isotope tracers suggests this pattern was largely driven by stimulated denitrification. Nitrate addition also significantly altered the microbial community and decreased alpha diversity, selecting for taxa belonging to groups known to reduce nitrate and oxidize more complex forms of organic matter. Fourier Transform-Infrared Spectroscopy further supported these results, suggesting that nitrate facilitated decomposition of complex organic matter compounds into more bioavailable forms. Taken together, these results suggest the existence of organic matter pools that only become accessible with nitrate and would otherwise remain stabilized in the sediment. The existence of such pools could have important implications for carbon storage, since greater decomposition rates as N loading increases may result in less overall burial of organic-rich sediment. Given the extent of nitrogen loading along our coastlines, it is imperative that we better understand the resilience of salt marsh systems to nutrient enrichment, especially if we hope to rely on salt marshes, and other blue carbon systems, for long-term carbon storage.
2003 Prescribed Burn Effect on Chihuahuan Desert Grasses and Shrubs at the Sevilleta National Wildlife Refuge, New Mexico: Grass Recovery Study (2003-2018)
The U.S. Fish and Wildlife's plan to apply a prescribed burn to a large portion of McKenzie Flats was deemed an opportunity to study the effects of fire on vegetation at the boundary between shrubland and grassland. This study actually was undertaken on an area that had prescribed fire applied to 8 of 16 (300 m x 300 m) plots 10 years before in 1993. This previous study had also examined the effects of fencing to exclude the indigenous prong-horn antelope. In the 2003 study the prescribed fire was applied to the northeastern half of the 16 plots while the southwestern plots were intentionally protected. Sampling prior to the prescribed burn included quantification of cover of grass species in quadrats within all of the 16 plots. Measurements were made using "niner" quadrat frames that are 30 cm x 30 cm frames that are divided into 9 1-decimeter squares. Counts of grass species were made just prior to the June 2003 burn. Following the prescribed burn, quadrats were remeasured in the fall of 2003 to quantify mortality of grass species. These measurements were taken through the fall of 2012 and the fall of 2018. Measurements were not taken for 2014-2017.
2003 Prescribed Burn Effect on Chihuahuan Desert Grasses and Shrubs at the Sevilleta National Wildlife Refuge, New Mexico: Grass Recovery Study (2003-2018)
The U.S. Fish and Wildlife's plan to apply a prescribed burn to a large portion of McKenzie Flats was deemed an opportunity to study the effects of fire on the vegetation at the boundary between shrubland and grassland. This study actually was undertaken on an area that had prescribed fire applied to 8 of 16 (300 m x 300 m) plots 10 years before in 1993. This previous study had also examined the effects of fencing to exclude the indigenous prong-horn antelope. In the 2003 study the prescribed fire was applied to the northeastern half of the 16 plots while the southwestern plots were intentionally protected. Sampling prior to the prescribed burn included quantification of fuel load (ie. the standing biomass of all grasses and forbs in the area to be burned). These measurements were made using Daubenmire quadrat frames that are 5 cm x 20 cm and delineate a 0.1 square meter area. Four samples were taken adjacent to the six 3 m x 4 m quadrats in each of the eight plots that were to be burned. Quadrat frames were laid down over the vegetation and all vegetation rooted within the frame was clipped at ground level. This material was bagged, oven-dried and weighed. Following the prescribed burn, re-measurements were made every fall of from 2004 until 2012 when vegetation had reached its annual peak biomass. Data were not collected from 2014-2017, but were collected again in 2018.
2003 Prescribed Burn Effect on Chihuahuan Desert Grasses and Shrubs at the Sevilleta National Wildlife Refuge, New Mexico: Species Composition Study (2004-2018)
Disturbance from fire can affect the abundance and distribution of shrubs and grasses in arid ecosystems. In particular, fire may increase grass and forb production while hindering shrub encroachment. Therefore, prescribed fires are a common management tool for maintaining grassland habitats in the southwest. However, Bouteloua eriopoda (black grama), a dominant species in Chihuahuan Desert grassland, is highly susceptible to fire resulting in death followed by slow recovery rates. A prescribed fire on the Sevilleta National Wildlife refuge in central New Mexico in 2003 provided the opportunity to study the effects of infrequent fires on vegetation in this region. This study was conducted along a transition zone where creosote bushes (Larrea tridentata) are encroaching on a black grama grassland. Before and after the fire, above ground plant productivity and composition were monitored from 2003 to present. Following the prescribed fire, there were fewer individual grass clumps and less above ground grass cover in burned areas compared to unburned areas. This decrease in productivity was primarily from a loss of B. eriopoda. Specifically, B. eriopoda density and cover were significantly lower following the fire with a slow recovery rate in the five years following the fire. Other grasses showed no such adverse response to burning. Data were collected from 2004-2013 and 2018. Data were not collected for 2014-2017.
Effect of Long-Term Nitrogen Fertilization on Mycorrhizal Fungi Associated with a Dominant Grass in a Chihuahuan Desert Grassland at the Sevilleta National Wildlife Refuge, New Mexico (2005)
We studied the diversity of arbuscular mycorrhizal fungi (AMF) in a semiarid grassland and the effect of long-term nitrogen (N) fertilization on this fungal community. Root samples of Bouteloua gracilis were collected at the Sevilleta National Wildlife Refuge (New Mexico, USA) from control and N-amended plots that have been fertilized since 1995. Small subunit rDNA was amplified using AMF specific primers NS31 and AM1. The diversity of AMF was low in comparison with other ecosystems, only seven operational taxonomic units (OTU) were found in B. gracilis and all belong to the genus Glomus. The dominant OTU was closely related to the ubiquitous G. intraradices/G. fasciculatum group. N-amended plots showed a reduction in the abundance of the dominant OTU and an increase in AMF diversity. The greater AMF diversity in roots from N-amended plots may have been the result of displacement of the dominant OTU, which facilitated detection of uncommon AMF. The long-term implications of AMF responses to N enrichment for plant carbon allocation and plant community structure remain unclear.
Effects of Kangaroo Rat Mounds on Seed Banks of Grass and Shrublands at the Sevilleta National Wildlife Refuge, New Mexico (2001)
Disturbance is a major factor in determining the spatial structure and temporal dynamics of ecological systems. Many studies have been conducted concerning the plant assemblages around Dipodmys spectabilis mounds compared to the off mound area. These studies have shown that annual plant cover is higher on the kangaroo rat mound compared to off the mound. However, no studies have addressed the effects of these rodents disturbance on the soil seed bank. Soil seed banks are an important component of the plant community particularly in arid environments. Annual plants have been known to create viable seeds that remain dormant in the soil for many years making their seed bank a persistent one. A persistent seed bank allows for future recruitment of plants given favorable conditions that could have a dramatic impact on the overall species diversity of the community. We studied the seed bank of eight forb taxa to ask the following questions: 1) Are there more seeds in the seed bank around kangaroo rat mounds compared to other microhabitats? 2) Does the seed composition differ among the different microhabitats? 3) If the seed composition does differ, do specific physical components of microhabitats predict seed populations?
Effects of Altered Precipitation on Biological Soil Crusts, Fungi, and Soil Nitrogen Availability at the Monsoon Rainfall Manipulation Experiment (MRME) in the Sevilleta National Wildlife Refuge, New Mexico (2016)
Microbial activity in drylands is mediated by the magnitude and frequency of growing season rain events that will shift as climate change progresses. Nitrogen is often co-limiting with water availability to dryland plants, and thus we investigated how microbes important to the nitrogen (N) cycle and soil N availability varied temporally and spatially in the context of a long-term rainfall variability experiment in the northern Chihuahuan Desert. Specifically, we assessed biological soil crust (biocrust) chlorophyll content, fungal abundance, and inorganic N in soils adjacent to individuals of the grassland foundation species, Bouteloua eriopoda, and in the unvegetated interspace at multiple time points associated with an experimental monsoon rain treatment. Treatments included small weekly (5 mm) or large monthly (20 mm) rain events, which had been applied during the summer monsoon for nine years prior to our sampling. Additionally, we evaluated target plant C:N ratios and added 15 N-glutamate to biocrusts to determine potential for nutrient transport to B. eriopoda. Biocrust chlorophyll was up to 67% higher in the small weekly or large monthly rainfall regimes compared to ambient controls. Fungal biomass was 57% lower in soil interspaces than adjacent to plants but did not respond to rainfall regime treatments. Ammonium and nitrate concentrations near plants declined through the sampling period but varied little in soil interspaces. There was limited movement of 15 N from interspace biocrusts to leaves but high 15 N retention in the soils even after additional ambient and experimental rain events. Plant C:N ratio was unaffected by rainfall treatments. The long-term alteration in rainfall regime in this experiment did not change how short-term microbial abundance or N availability responded to the magnitude or frequency of events, suggesting a limited response of N availability to future climate change.
Effect of foot disturbance to cyanobacteria-dominated biocrusts on microbes and nitrogen in Chihuhuahan grassland and shrubland
Interactions between plants and soil microbes influence plant nutrient transformations, including nitrogen (N) fixation, nutrient mineralization, and resource exchanges through fungal networks. Physical disturbances to soils can disrupt soil microbes and associated processes that support plant and microbial productivity. In low resource drylands, biological soil crusts ("biocrusts") occupy surface soils and house key autotrophic and diazotrophic bacteria, non-vascular plants, or lichens. Interactions among biocrusts, plants, and fungal networks between them are hypothesized to drive carbon and nutrient dynamics; however, comparisons across ecosystems are needed to generalize how soil disturbances alter microbial communities and their contributions to N pools and transformations. To evaluate linkages among plants, fungi, and biocrusts, we disturbed all unvegetated surfaces with human foot trampling twice yearly in dry conditions from 2013-2018 in cyanobacteria-dominated biocrusts in Chihuahuan Desert grassland and shrubland ecosystems. Our study included microbial communities and N pools sampled at different time points in the disturbance treatments at one or both sites. We began our sampling after observations in April 2018 that the chlorophyll a content was at least double in control than disturbed plots in both ecosystems (Chung et al. 2019). Stomping occurred in May, and we collected soil and plant samples in June 2018 for N pools and soil and root fungal abundance. We collected additional soil samples in September 2018 and conducted the 15N tracer experiment to observe rates of N transfer from biocrust to plants before the fall stomp treatment in October. We collected chlorophyll a samples and soils for sequencing bacteria in September of 2019, also before the fall stomp treatment.
Salinity and Simulated Herbivory Effects on Spartina alterniflora in a Mesocosm, 2017
The goal of this mesocosm study was to examine the effects of salinity (0, 6,14, 19 or 26 ppt), collection site (mesohaline vs. freshwater marsh), and simulated herbivory via clipping on the growth and plant traits of Spartina alterniflora. Spartina alterniflora collected from a mesohaline marsh (Taskinas Creek) and a freshwater marsh (Sweet Hall) was grown in mesocosms and subjected to one of five salinities and an herbivory treatment. Chlorophyll concentrations, total phenolic concentrations, total soluble protein content, belowground biomass, number of new shoots produced, and carbon and nitrogen content were assessed.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.