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Supplementary material 1 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Table S1–S5

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Figure 8 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 8 The holotype adult male CIBMT171053 of Megophrys zhoui sp. nov. in life. A dorsolateral view of body B ventral view of body C dorsolateral view of head D ventral view of hand E ventral view of foot.

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Figure 7 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 7 The adult male CIB022017061805 of M. cf. pachyproctus in life. A dorsolateral view of body B ventral view of body C dorsolateral view of head D ventral view of hand E ventral view of foot.

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Figure 5 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 5 Skull of Megophrys species in Medog. A–E dorsal views of adult male holotype NWIPB770650 of M. pachyproctus, adult male topotype CIB022017061406 of M. medogensis, adult male CIB022017061805 of M. cf. pachyproctus, adult male holotype CIBMT171053 of Megophrys zhoui sp. nov., and adult male holotype CIB201706MT02 of Megophrys yeae sp. nov., respectively F–J ventral views of the specimens, respectively. Key to skull: 1 premaxillary; 2 maxillary; 3 nasal; 4 sphenethmoid; 5 anterior fontanelle; 6 frontoparietal; 7 sagittal suture; 8 pterygoid; 9 squamosal; 10 quadratojugal; 11 prootic; 12 exoccipital; 13 vomerine ridge; 14 mandible; 15 anterior process of parasphenoid; 16 columella auris. Scale bar equal to 5 mm.

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Figure 4 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 4 Visualization of advertisement calls of three Megophrys species from Medog. A–C visualizations of 60 seconds waveform of relative amplitude over time for M. medogensis (one unvouchered individual recorded in the vicinity of Medog urban area), M. cf. pachyproctus (CIB022017061807), and Megophrys yeae sp. nov. (paratype CIB022017061804), respectively D–F visualizations of 20 seconds waveform of relative amplitude over time G–I visualizations of two seconds waveform of relative amplitude for the species, respectively J–L visualizations of two seconds waveform of spectrogram for the species, respectively.

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Figure 3 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 3 Photos of specimens of Megophrys species in Medog. A–E dorsal views of adult male holotype NWIPB770650 of M. pachyproctus, adult male topotype CIB022017061406 of M. medogensis, adult male CIB022017061805 of M. cf. pachyproctus, adult male holotype CIBMT171053 of Megophrys zhoui sp. nov., and adult male holotype CIB201706MT02 of Megophrys yeae sp. nov., respectively F–J ventral views of the specimens, respectively K–O lateral view of head of the specimens, respectively P–T ventral view of hand of the specimens, respectively U–Y ventral view of foot of the specimens, respectively. Scale bar for body view equal to 10 mm, and for partial view 5 mm.

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Figure 2 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 2 Phylogenetic trees respectively based on the mitochondrial DNA and nuclear DNA. A Maximum Likelihood (ML) tree based on the mitochondrial DNA BML tree based on the nuclear DNA. ML bootstrap support/Bayesian posterior probability was denoted beside node. Samples 1–144 refer to Suppl. material 1: Table S1.

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Figure 6 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 6 Tadpole specimens of four groups of three Megophrys species from Medog. A–D dorsal views of the low-middle-elevation tadpole CIBMT20170621 of M. medogensis (Goser stage 35), the high-elevation tadpole CIBMT171001 of M. medogensis (stage 27), tadpole CIBMT20170611 of M. cf. pachyproctus (stage 25), tadpole CIBMT170604 of Megophrys yeae sp. nov. (stage 35), respectively E–H lateral views of the tadpoles, respectively I–L ventral views of the tadpoles, respectively. M–P dorsal views of head of the tadpoles, respectively. Scale bar for body view equal to 10 mm, and for head view 2 mm.

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Figure 1 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 1 Distributional localities for specimens of the Megophrys species used in this study in Medog County, Tibet Autonomous Region, China. 1 80k 2 Gedang village 3 vicinity of Medog urban area 4 Bari village 5 vicinity of Renqingbeng Temple 6 Beibeng village 7 Gelin village 8 Didong village 9 Yarang village. Species were denoted as different color.

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Figure 10 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 10 Microhabitats of Megophrys toads in the field in Medog. A stream at elevation 850 m in Didong village, harboring the low-middle-elevation M. medogensis and Megophrys yeae sp. nov. B a stream at 1530 m in Gelin village, hosting the low-middle-elevation M. medogensis and M. pachyproctusC a stream at 1780 m in Bari village, harboring low-middle-elevation M. medogensis, M. cf. pachyproctus and Megophrys yeae sp. nov. D a stream at 2003 m in the vicinity of Renqingbeng Temple, hosting M. cf. pachyproctus and Megophrys zhoui sp. nov. E a stream at 2142 m in Gedang village, hosting M. medogensisF one adult male of low-middle-elevation M. medogensis calling on a dead leaf on the tropical forest ground nearby a stream in Didong village G the adult male paratype CIB022017061102 of Megophrys yeae sp. nov. calling on a leaf of dense bushes under tropical forest, ca. 0.5 m above a stream in Didong village H the adult male CIB022017061806 of M. cf. pachyproctus calling on a branch of dead bush, ca. 0.5 m above ground under subtropical forest in Bari village I the gravid female CIBMT171054 of M. cf. pachyproctus precariously climbing up onto a stem of herb, ca. 0.3 m above a tiny stream under subtropical forest in Renqingbeng J the adult male holotype CIBMT171053 of Megophrys zhoui sp. nov. sitting on a split of a fern leaf in a small stream under subtropical forest in the vicinity of Renqingbeng Temple.

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Figure 9 from: Shi S, Zhang M, Xie F, Jiang J, Liu W, Li Ding1, Luan L, Wang B (2020) Multiple data revealed two new species of the Asian horned toad Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from the eastern corner of the Himalayas. ZooKeys 977: 101-161. https://doi.org/10.3897/zookeys.977.55693

Figure 9 The holotype adult male CIB201706MT02 of Megophrys yeae sp. nov. in life. A dorsolateral view of body B ventral view of body C dorsolateral view of head D ventral view of hand E ventral view of foot.

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Supplementary material 1 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Table S1. Uncorrected p-distances between the Megophrys species based on the 16S gene sequences.

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Figure 7 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Figure 7 Habitats of Megophrys anlongensis sp. nov. in the type locality, Anlong County, Guizhou Province, China A landscape of montane forests in the type locality B a mountain stream where toads of the new species live (insert the holotype standing on the leaf beside the stream).

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Supplementary material 2 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Table S2. Uncorrected p-distances between the Megophrys species based on the COI gene sequences

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Figure 6 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Figure 6 Visualisation of advertisement calls of Megophrys anlongensis sp. nov. A waveform showing one note B sonogram showing one note C waveform showing 20 notes of one call D sonogram showing 20 notes of one call.

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Figure 5 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Figure 5 Colour variation in Megophrys anlongensis sp. nov. A dorsolateral view of the specimen CIBAL20190531017 B ventral view of the male specimen CIBAL20190531017 C dorsolateral view of the specimen CIBAL20190531022 D ventral view of the specimen CIBAL20190531022 E dorsolateral view of the specimen CIBAL20190811014 F ventral view of the specimen CIBAL20190811014.

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Figure 4 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Figure 4 Photographs of the holotype specimen CIBAL20190531018 of Megophrys anlongensis sp. nov. A dorsal view B ventral view C lateral view D ventral view of hand E ventral view of foot.

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Figure 3 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Figure 3 Photographs of the holotype CIBAL20190531018 of Megophrys anlongensis sp. nov. in life A dorsal view B ventral view C dorsal view of hand D ventral view of hand. E ventral view of foot.

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Figure 2 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Figure 2 Maximum likelihood (ML) tree of the genus Megophrys reconstructed based on the 16S rRNA and COI gene sequences. Bayesian posterior probability/ML bootstrap supports were denoted beside each node. Samples 1–90 refer to Table 2.

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Figure 1 from: Li S-Z, Lu N-N, Liu J, Wang B (2020) Description of a new Megophrys Kuhl & Van Hasselt, 1822 (Anura, Megophryidae) from Guizhou Province, China. ZooKeys 986: 101-126. https://doi.org/10.3897/zookeys.986.57119

Figure 1 Geographical location of the type locality of Megophrys anlongensis sp. nov., Anlong County, Guizhou Province, China.

opencc-by-4.0Nov 2020View details →

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