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621 results for “methodologies”
Overview of the LIFE Methodology video
<p>Overview of the LIFE Methodology video</p> <p>https://youtu.be/OCdzrxJ1zyo</p>
Data from: From environmental DNA sequences to ecological conclusions: how strong is the influence of methodological choices?
Aim: Environmental DNA (eDNA) is increasingly used for analysing and modelling all-inclusive biodiversity patterns. However, the reliability of eDNA-based diversity estimates is commonly compromised by arbitrary decisions for curating the data from molecular artefacts. Here, we test the sensitivity of common ecological analyses to these curation steps, and identify the crucial ones to draw sound ecological conclusions. Location : Valloire, French Alps. Taxon: Vascular plants and Fungi. Methods: Using soil eDNA metabarcoding data for plants and fungi from twenty plots sampled along a 1000-m elevation gradient, we tested how the conclusions from three types of ecological analyses: (i) the spatial partitioning of diversity, (ii) the diversity-environment relationship, and (iii) the distance-decay relationship, are robust to data curation steps. Since eDNA metabarcoding data also comprise erroneous sequences with low frequencies, diversity estimates were further calculated using abundance-based Hill numbers, which penalize rare sequences through a scaling parameter, namely the order of diversity q (Richness with q=0, Shannon diversity with q~1, Simpson diversity with q=2). Results: We showed that results from different ecological analyses had varying degrees of sensitivity to data curation strategies and that the use of Shannon and Simpson diversities led to more reliable results. We demonstrated that MOTU clustering, removal of PCR errors and of cross-sample contaminations had major impacts on ecological analyses. Main conclusions: In the Era of Big Data, eDNA metabarcoding is going to be one of the major tools to describe, model and predict biodiversity in space and time. However, ignoring crucial data curation steps will impede the robustness of several ecological conclusions. Here, we propose a roadmap of crucial curation steps for different types of ecological analyses.
Figure 2 from: Tran VD, Dorofeeva VV, Loskutova EE, Lagutkina TP, Kosova IV, Gribkova EI, Galkina GA, Pak TV, Ibragimova AN, Le MH, Cao TTN (2020) Elderly consumers' satisfaction with the quality of community pharmacy services in Ho Chi Minh City, Vietnam: a Q-methodology study. Pharmacia 67(4): 303-309. https://doi.org/10.3897/pharmacia.67.e56511
Figure 2 Scree plot and parallel analysis.
Data from: Comparison of methodological quality of positive versus negative comparative studies published in Indian medical journals: a systematic review
Objectives: Published negative studies should have the same rigour of methodological quality as studies with positive findings. However, the methodological quality of negative versus positive studies is not known. The objective was to assess the reported methodological quality of positive versus negative studies published in Indian medical journals. Design: A systematic review (SR) was performed of all comparative studies published in Indian medical journals with a clinical science focus and impact factor >1 between 2011 and 2013. The methodological quality of randomised controlled trials (RCTs) was assessed using the Cochrane risk of bias tool, and the Newcastle-Ottawa scale for observational studies. The results were considered positive if the primary outcome was statistically significant and negative otherwise. When the primary outcome was not specified, we used data on the first outcome reported in the history followed by the results section. Differences in various methodological quality domains between positive versus negative studies were assessed by Fisher's exact test. Results: Seven journals with 259 comparative studies were included in this SR. 24% (63/259) were RCTs, 24% (63/259) cohort studies, and 49% (128/259) case–control studies. 53% (137/259) of studies explicitly reported the primary outcome. Five studies did not report sufficient data to enable us to determine if results were positive or negative. Statistical significance was determined by p value in 78.3% (199/254), CI in 2.8% (7/254), both p value and CI in 11.8% (30/254), and only descriptive in 6.3% (16/254) of studies. The overall methodological quality was poor and no statistically significant differences between reporting of methodological quality were detected between studies with positive versus negative findings. Conclusions: There was no difference in the reported methodological quality of positive versus negative studies. However, the uneven reporting of positive versus negative studies (72% vs 28%) indicates a publication bias in Indian medical journals with an impact factor of >1.
Data from: Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Background: Over the past couple of decades, measuring immunological parameters has become widespread in studies of ecology and evolution. A combination of different immunological indices is useful for quantifying different parts of the immune system and comprehensively assessing immune function. Running multiple immune assays usually requires samples to be repeatedly thawed and re-frozen. There is some evidence that repeated freezing and thawing can affect assay results, but this has never been comprehensively studied in some common ecological immunology assays. We tested the effect of multiple (1, 2, 3, 4, 5, 10) freeze-thaw cycles on the results of four commonly used immunological assays: haemolysis-haemagglutination titres, haptoglobin concentration, bacterial killing capacity and total immunoglobulins (IgY). We tested five different bird species from four different bird orders (Passeriformes, Columbiformes, Charadriiformes and Galliformes), and we included both captive and free-living individuals. In addition, we tested for haptoglobin concentrations and the haemolysis-haemagglutination assay if re-analysing samples 1 year apart led to different results. For the haemolysis-haemagglutination assay we also tested two different sources of rabbit blood, and we compared untreated microtitre plates with plates that were "blocked" to prevent nonspecific interactions between the plate and assay reagents. Results: Repeated freezing and thawing of plasma had no effect on lysis titres, haptoglobin concentrations, bacterial killing capacity, or total immunoglobulin levels. Agglutination titres were unaffected by up to five cycles but were lower after ten freeze-thaw cycles. For the haemolysis-haemagglutination assay and haptoglobin concentrations, re-analysing samples 1 year apart yielded highly correlated data. For the haemolysis haemagglutination assay,thesourceofrabbit blood did not influence the results, and the untreated vs. blocked plates differed slightly overall, but at the individual level assay results were highly correlated. Using different rabbit blood sources or different types of microtitre plates yielded highly correlated data.Conclusions: Our data suggest that repeated freeze-thaw cycles do not impair assay results to the point of influencing ecological or evolutionary conclusions. Plasma samples can be safely stored in one tube and thawed repeatedly for different assays. Nevertheless, we recommend consistent treatment of samples in terms of freeze-thaw cycles or other laboratory treatments to minimize the potential for introducing a systematic bias.
The development of unlike induced association-site models to study the phase behaviour of aqueous mixtures comprising acetone, alkanes and alkyl carboxylic acids with the SAFT-g Mie group contribution methodology
<p>All computational data presented in the figures.</p>
METHODOLOGICAL FOUNDATIONS OF TEACHING ENGLISH IN THE HIGHER EDUCATIONAL INSTITUTIONS BASED ON THE CLUSTER APPROACH
Open the record for dataset details and reuse information.
A generic methodology for mapping the performances of heat pumps considering part-load behaviour - Supplementary material
<h2>Description</h2> <p>This is the supplementary material provided with the journal article "A generic methodology for mapping the performances of heat pumps considering part-load behaviour" published in Energy and Buildings on 2024, September 01. (<a href="https://doi.org/10.1016/j.enbuild.2024.114490">https://doi.org/10.1016/j.enbuild.2024.114490</a>).</p> <h2>Content</h2> <ul> <li>Datasets are provided as NetCDF files for European maps</li> <li>SCOP/SEER estimations at TMY locations are provided as CSV files</li> <li>COP/EER cruves at TMY locations are provided as CSV files</li> <li>NUTS aggregates of SCOP and Peak COP are provided as CSV files</li> <li>European maps are provided as PDF files.</li> </ul> <h2>Code availability</h2> <p>The code used to generate these datasets is openly available on GitLab (<a href="https://gitlab.com/antoine.rogeau/generic-heatpump-model.git">https://gitlab.com/antoine.rogeau/generic-heatpump-model.git</a>) or on Zenodo (<a title="https://doi.org/10.5281/zenodo.10594143" href="https://doi.org/10.5281/zenodo.10594143">https://doi.org/</a><a title="https://doi.org/10.5281/zenodo.10594143" href="https://doi.org/10.5281/zenodo.10594143">10.5281/zenodo.10594143</a>)</p> <div> <div> <div> </div> <div> <div> <div> </div> <div> <p> </p> <p> </p> </div> </div> </div> </div> </div>
Data from: Towards a Methodology for Technoscientific Objects Extraction (Short Paper)
<p>This dataset includes data adopted in the paper "Towards a Methodology for Technoscientific Objects Extraction (Short Paper)". </p> <p>The file "articles_urls_2022.txt.zip" is an archive containing a txt file; each line of the txt file is an article URL. </p> <p>The libraries adopted to process the articles are reported in the paper or in the paper references. </p>
Development of a CSRML version of the Analog Identification Methodology (AIM) fragments and their evaluation within the Generalised Read-Across (GenRA) approach
<p>Datasets supporting manuscript Adams M, Hidle H, Chang D, Richard AM, Williams AJ, Shah I, Patlewicz G*. Development of a CSRML version of the Analog Identification Methodology (AIM) fragments and their evaluation within the Generalised Read-Across (GenRA) approach. Computational Toxicology, 2022, <em>in press</em>. <a href="https://doi.org/10.1016/j.comtox.2022.100256" rel="nofollow">https://doi.org/10.1016/j.comtox.2022.100256</a></p>
CO2 reforming with methane for syngas production using a dielectric barrier discharge plasma coupled with Ni/γ-Al2O3 catalysts: Process optimization through response surface methodology
<p>XRD data, TG data, experimental data</p>
Figure 2 in Haematopoiesis in the European common toad Bufo bufo (Linnaeus, 1758): new methodological insights to study general, seasonal, and sexual haematopoietic distribution and maturation pattern
Figure 2. Liver of adult Bufo bufo (A- section II, B- section I).
THE METHODOLOGY OF TEACHING BASED ON THE TEXT OF THE SPECIALTY
<p><em><span>This article highlights the essence, advantages and effective ways to apply in practice text-based teaching methods in the specialty. Text-based teaching is an important part of the modern educational process for students to gain in-depth knowledge in their field. The article outlines the specific aspects of teaching methods through text selection, analysis, discussion and practical assignments, and the importance of students in developing critical thinking skills. Proposals are also made to improve the effectiveness of this approach in the educational process.</span></em></p>
Implementation of HBB Training With Facilitators That Have Undergone Simulation Methodology Training
ClinicalTrials.gov study NCT03256591. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Dialysis Patients' Perspectives: A Study of the Q Methodology
ClinicalTrials.gov study NCT07326306. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Scientific Justification of Assessment Criteria and Improvement of the Methodology for Assessing School Maturity
ClinicalTrials.gov study NCT05736159. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Which Automated Methodology?
ClinicalTrials.gov study NCT06049056. IPD Sharing: NO. Countries: 1. Publications: 0.
A Pivotal Study to Assess the Safety and Efficacy of The PrePex System, a Male Circumcision Device and Methodology for Rapid Scale up of Painless and Bloodless National Circumcision Programs, in Urban
ClinicalTrials.gov study NCT01150370. IPD Sharing: Not stated. Countries: 1. Publications: 0.
A Pilot Methodology Study To Evaluate Changes In Bone Quality Parameters Following Therapy With Forteo
ClinicalTrials.gov study NCT00365924. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Alternate Methodology of Pulse Oximeter Validation
ClinicalTrials.gov study NCT03628560. IPD Sharing: NO. Countries: 1. Publications: 0.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.