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6,171 results for “mutant”
During development the Sku5 mutant roots engage different genes than wild type WS roots either on the ground or in spaceflight.
The wild type WS plants and the Sku5 mutant plants in the WS background were germinated on ISS or on the ground and the gene expression profiles in roots at 4 days or 8 days were established. The Sku5 gene (AT4G12420) codes for a multi-copper oxidase-like protein SKU5 protein that is involved in directed root tip growth. The Sku5 protein is glycosylated GPI-anchored and localizes to the plasma membrane and the cell wall. The Sku5 gene is expressed most strongly in expanding tissues. The development of WS and Sku5 plants on orbit differs from that on the ground as demonstrated by the comparison of the gene expression profiles between 4 days old to 8 days old plant in the two environments. However the development of Sku5 mutant plants also differs from WS at either age and in either environment suggesting the role of the genetic background in these developmental decisions. The 4 days old Sku5 roots in orbit engaged substantially more genes than the 4 days old WS roots in orbit but also more than the 4 days old Sku5 roots on the ground. Overall the 4 days old roots differentially expressed more genes in spaceflight relative to ground than the 8 days old roots of either genotype. Finally the 4 days old Sku5 roots in orbit differ in 862 genes from the 8 days Sku5 roots in orbit while on the ground there is merely half of the number of genes differentially expressed between the 4 days and 8 days developmental stages of the Sku5 roots. APEX03-2 (Advanced Plant Experiment 03-2) also identified as TAGES-Isa (Transgenic Arabidopsis Gene Expression System xe2 x80 x94Intracellular Signaling Architecture) was launched on SpaceX mission CRS-5 on 10 January 2015. Dry sterilized Arabidopsis seeds were planted aseptically on the surface of 10-cm2 solid media plates and remained dormant until removed from cold stowage and exposed to light at the initiation of the experiment on the ISS (International Space Station). The plates were grown in the Vegetable Production System (VPS/Veggie) hardware on the Columbus Module of the ISS with the overhead LED lighting of the VPS. At four or for the second experimental set at eight days seedlings were harvested by an astronaut into KFT (Kennedy Fixation Tube) containing RNAlater solutions. Upon return to Earth the harvested material was used to compare the transcriptomes of each genotype and each age using RNAseq technology. The patterns of gene expression was compared between treatments (spaceflight versus ground control) within each genotype of same age or between two ages within same genotype and within same treatment. For 4 day old roots 5-8 roots were combined to serve as one biological replica. For 8 day old roots 2-3 roots were used to form one biological replica. In each case the four biological replicas were used for the transcriptomic analysis.
RNAseq analysis of the response of Arabidopsis thaliana phytochrome mutants (PhyA PhyB) to fractional gravity under blue-light stimulation during spaceflight
Traveling to nearby extraterrestrial objects having a reduced gravity level (partial gravity) compared to Earth s gravity is becoming a realistic objective for space agencies. The use of plants as part of life support systems will require a better understanding of the interactions among plant growth responses including tropisms under partial gravity conditions. Here we present results from the Seedling Growth space experiments on the ISS to complement the previously released GLDS-251 dataset including seeds of Arabidopsis thaliana wildtype plants. Seeds were germinated and seedlings grew for six days under different gravity levels namely micro-g several intermediate partial-g levels and 1g and were subjected to irradiation with blue light for the last 48 hours. RNA was extracted was obtained for 20 wildtype samples for subsequent RNAseq analysis in GLDS-251 here we add 36 samples from similarly exposed PhyA and PhyB mutants.
Synergistic effects of space radiation and microgravity (miRNA WT and ced-1 mutant)
Space radiations and microgravity both could cause DNA damage in cells but the effects of microgravity on DNA damage response to space radiations are still controversial. A mRNA microarray and microRNA microarray in dauer larvae of Caenorhabditis elegans (C. elegans) that endured spaceflight environment and space radiations environment during 16.5-day Shenzhou-8 space mission were performed. In our study wild type and ced-1 mutant strains of C.elegans endured three conditions during shenzhou-8 spaceflight mission including spaceflight static condition(ss) spaceflight 1-g centrifugal condition(sc) and ground control condition(gc). Limited to the quantity of worm samples we performed technical-repeat test but not sample-repeat test.Accordingly xef xbc x8csix miRNA microarrays were performed.
Transcription profiling of atm mutant adm mutant and wild type whole plants and roots of Arabidopsis after gamma ray irradiation in a time series
Whole seedlings of wild type (4d) and atm mutants (4d) have been analyzed after a gamma ray irradiation of 0.75h 1.5h 3h & 5h (time course). Roots of wt (4d) atm (3d) and atr (4d) mutants have been analyzed after a 1h irradiation. Ataxia Telangiectasia Mutated (ATM) encodes a large protein with a phosphatidylinositol 3-kinase (PI3K)-like domain at the C terminus (reviewed by Rotman and Shiloh 1998). PI3K-related proteins make up a large family of Ser-Thr protein kinases numerous members of which are involved in the regulation of cell cycle progression responses to DNA damage and the maintenance of genomic stability (Hoekstra 1997). AtATM plays an essential role in meiosis and in the somatic response to DNA damage in plants similar to the function of ATM in mammals and other eukaryotes. Ataxia telangiectasia-mutated and Rad3-related (ATR) plays a central role in cell-cycle regulation transmitting DNA damage signals to downstream effectors of cell-cycle progression.
Mouse ES-derived motor neurons (ES-MN) exposed to SOD1 mutant mouse astrocyte conditioned media
GEO Series GSE49023. Mus musculus. 36 samples. Type: Expression profiling by high throughput sequencing.
Expression Data human IDH1 mutant and wild-type high grade gliomas
GEO Series GSE104722. Homo sapiens. 20 samples. Type: Expression profiling by array.
Circular RNAs are down-regulated in KRAS mutant colon cancer cells and can be transferred to exosomes
GEO Series GSE72577. Homo sapiens. 15 samples. Type: Expression profiling by high throughput sequencing.
Drosophila esc mutants show Hox-derepression along the AP axis in CNS
GEO Series GSE111324. Drosophila melanogaster. 8 samples. Type: Expression profiling by high throughput sequencing.
Gene expression signature of WT siblings and hif1α mutants in normoxia and after hypoxia chamber incubation
GEO Series GSE89117. Danio rerio. 4 samples. Type: Expression profiling by array.
CGH to ascertain levels of gDNA in third instar salivary glands of various mutant Drosophila
GEO Series GSE31898. Drosophila melanogaster. 5 samples. Type: Genome variation profiling by genome tiling array.
Next-generation sequencing analyses of the differently expressed genes of wild type and atcep1 mutant Arabidopsis thaliana
GEO Series GSE50956. Arabidopsis thaliana. 6 samples. Type: Expression profiling by high throughput sequencing.
RNAseq comparison of fadR deletion mutant and the wild type strain
GEO Series GSE108018. Sulfolobus acidocaldarius DSM 639. 4 samples. Type: Expression profiling by high throughput sequencing.
Expression data in an Arabidopsis cytokinin (CK)-signaling mutant, ahp2,3,5 and its wild type plant (Col-0) under drought and well-water (control) conditions.
GEO Series GSE42290. Arabidopsis thaliana. 12 samples. Type: Expression profiling by array.
SmallRNA-SEQ of mutants B cell for IgH 3'RR and Emu
GEO Series GSE90759. Mus musculus. 10 samples. Type: Non-coding RNA profiling by high throughput sequencing.
mRNA-seq of Drosophila Ago2 mutants
GEO Series GSE51136. Drosophila melanogaster. 4 samples. Type: Expression profiling by high throughput sequencing.
RNA-Seq analysis of wild-type and ttpA-C231S, ttpA-E236fsx, and ttpA-Y242fsx mutant D. discoideum amebae
GEO Series GSE156809. Dictyostelium discoideum. 16 samples. Type: Expression profiling by high throughput sequencing.
Base editing effectively prevents the early-onset hypertrophic cardiomyopathy in Mybpc3 mutant mice
GEO Series GSE239872. Mus musculus. 9 samples. Type: Expression profiling by high throughput sequencing.
Restoration of the Tumor Suppressor Function of Y220C-Mutant p53 in vivo by Rezatapopt (PC14586), a Small Molecule Reactivator [in vivo]
GEO Series GSE276224. Homo sapiens. 48 samples. Type: Expression profiling by high throughput sequencing.
Salmonella Typhimurium: wild type vs. ∆scsA mutant
GEO Series GSE30924. Salmonella enterica subsp. enterica serovar Typhimurium str. DT104; Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; Salmonella enterica subsp. enterica serovar Enteritidis; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344; Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91. 12 samples. Type: Expression profiling by array.
mRNA sequencing of MDA-MB-231 engineered for doxycycline-inducible overexpression of wild-type or mutant myocardin related transcription factor A
GEO Series GSE253047. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.