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5,412 results for “Cell differentiation”
Dynamic 3D Genome and Epigenetic Landscapes of Macrophage Cells Reprogramming Gene Expression during Differentiation and Infection [sciDLO Hi-C]
GEO Series GSE159501. Homo sapiens. 3 samples. Type: Other.
Mll3/Mll4 catalytic activity is necessary for de novo enhancer-promoter contacts during embryonic stem cell differentiation
GEO Series GSE160892. Mus musculus. 131 samples. Type: Other; Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
Dataset related to article "Subpopulations of Circulating Cells with Morphological Features of Malignancy Are Preoperatively Detected and Have Differential Prognostic Significance in Non-Small Cell Lung Cancer "
<p>This record contains raw data related to article “Subpopulations of Circulating Cells with Morphological Features of Malignancy Are Preoperatively Detected and Have Differential Prognostic Significance in Non-Small Cell Lung Cancer"</p> <p><strong>Background: </strong> Non-small cell lung cancer (NSCLC) frequently presents when surgical intervention is no longer feasible. Despite local treatment with curative intent, patients might experience disease recurrence. In this context, accurate non-invasive biomarkers are urgently needed. We report the results of a pilot study on the diagnostic and prognostic role of circulating tumor cells (CTCs) in operable NSCLC.</p> <p><strong>Methods: </strong> Blood samples collected from healthy volunteers (<em>n</em> = 10), nodule-negative high-risk individuals enrolled in a screening program (<em>n</em> = 7), and NSCLC patients (<em>n</em> = 74) before surgery were analyzed (4 mL) for the presence of cells with morphological features of malignancy enriched through the ISET<sup>®</sup> technology.</p> <p><strong>Results: </strong> CTC detection was 60% in patients, while no target cells were found in lung cancer-free donors. We identified single CTCs (sCTC, 46%) and clusters of CTCs and leukocytes (heterotypic clusters, hetCLU, 31%). The prevalence of sCTC (sCTC/4 mL ≥ 2) or the presence of hetCLU predicted the risk of disease recurrence within the cohort of early-stage (I-II, <em>n</em> = 52) or advanced stage cases (III-IVA, <em>n</em> = 22), respectively, while other tumor-related factors did not inform prognosis.</p> <p><strong>Conclusions: </strong> Cancer cell hematogenous dissemination occurs frequently in patients with NSCLC without clinical evidence of distant metastases, laying the foundation for the application of cell-based tests in screening programs. CTC subpopulations are fine prognostic classifiers whose clinical validity should be further investigated in larger studies.</p>
HuD regulates SOD1 expression during oxidative stress in differentiated neuroblastoma cells and sporadic ALS motor cortex
<p><strong>The database includes </strong>the raw of the article “HuD regulates SOD1 expression during oxidative stress in differentiated neuroblastoma cells and sporadic ALS motor cortex”.</p> <p><strong>In detail, the database contains</strong> data obtained by the following evaluations: i) RNA electrophoretic mobility shift assay (REMSA) to detect the formation of a complex between HuD recombinant protein and SOD1 ARE sequences. ii) multiplex qRT-PCR to evaluate if oxidative stress levels, i.e H2O2 treatment, induces a shift in the alternative polyadenylation (APA) site usage in SOD1 3'UTR.</p> <p><strong>Aim of the work</strong> was to investigate the potential involvement of HuD (ELAVL4) one of the neuronal members of the ELAVL family, in SOD1 regulation during oxidative stress and in sporadic ALS patients (sALS).</p> <p>Using differentiated SH-SY5Y cells along with brain tissues from sALS patients, we evaluated HuD-dependent regulation of SOD1 mRNA. By means in vitro binding and mRNA decay assays we observed that HuD specifically binds to SOD1 ARE motifs promoting mRNA stabilization. In SH-SY5Y cells, we faound that the overexpression of full-length HuD increased SOD1 mRNA and protein levels; moreover HuD regulation of SOD1 mRNA we found that this regulation is related to oxidative stress, as shown after H2O2 exposure. H2O2 exposure also induced a shift in the APA site usage in SOD1 3'UTR, increasing the levels of a long variant bearing HuD binding sites. We validated this data using a specific siRNA. In the motor cortex from sALS patients, we found increases in SOD1 and HuD mRNAs and proteins, accompanied by greater HuD binding to this mRNA as confirmed by RNA-immunoprecipitation (RIP) assays.</p> <p><strong>To conclude,</strong> these results point a role of HuD in the post-transcriptional regulation of SOD1 expression in neurons after oxidative stress and in sALS brain tissues, thus suggesting an involvement in ALS pathogenesis.</p>
Patients' stem cells differentiation in a 3D environment as a promising experimental tool for the study of Amyotrophic Lateral Sclerosis
<p>The database includes the raw data of the article “Patients’ stem cells differentiation in a 3D environment as a promising experimental tool for the study of Amyotrophic Lateral Sclerosis”. Aim of this work was to demonstrate the validity of 3D cultures for the differentiation of stem cells for the study of Amyotrophic Lateral Sclerosis in a more realistic model.</p> <p>The database contains the data obtained by the following evaluations: a) immunofluorescence analysis for the study of cells’ proliferation during differentiation b) confocal microscopy for the evaluation of differentiation markers c) RT-qPCR for the confirmation of stem cells’ differentiation d) immunofluorescence analysis for the evaluation of the electrophysiological characteristics of differentiated NSC34 cells.</p> <p>We first reprogrammed healthy and sALS PBMCs in iPSCs and differentiated them in NSCs in 2D. NSCs were then included in Cellink Bioink and printed. We differentiated NSCs first in MNPs and finally in MNs and evaluated every step of 3D differentiation in comparison with 2D. We found that NSCs maintain a good viability and proliferation rate during the differentiation in 3D. Moreover, we confirmed the good differentiation by confocal microscopy and RT-qPCR by the use of specific markers for every specific differentiation step. We concluded that cell’s inclusion in the hydrogel does not interfere with the correct differentiation process. Moreover, Bioprinting allows the study of cells in a 3D environment which better mimics tissue organization. Furthermore, RT-qPCR results suggest that the 3D environment increases the differentiation of CTRL MNPs in mature MNs when compared to 2D.</p> <p>Finally, we evidenced that the electrophysiological characteristics of cells are maintained after the inclusion and the printing.</p> <p>Such evidence confirmed that 3D Bioprinting can be considered a good model for the study of ALS pathogenesis.</p>
Dataset for Mechanically Flexible Polymeric Nanoneedle Arrays for Promoting Differentiation and Functional Activity of Neural Progenitor Cells
Open the record for dataset details and reuse information.
Dcp1a, a novel Mek substrate, regulates the self-renewal and differentiation of mouse embryonic stem cells
GEO Series GSE269161. Mus musculus. 6 samples. Type: Other.
T-bet Suppresses TGF-b-independent Gut-resident Memory CD8+ T Cell Differentiation.
GEO Series GSE184629. Mus musculus. 22 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
hnRNPA2B1 promotes osteogenesis differentiation of human adipose-derived stem cells through PHC2/GATA6/BMP axis [CUT&RUN]
GEO Series GSE291175. Homo sapiens. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
H3K9 methyltransferase G9a negatively regulates UHRF1 transdcription during leukemia cell differentiation.
GEO Series GSE63149. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Neuroblastoma Neuro2A Cell Differentiation induced by retinoic acid-treatment
GEO Series GSE2570. Mus musculus. 38 samples. Type: Expression profiling by array.
The non-steroidal anti-inflammatory drugs Sulindac sulfide and Diclofenac induce apoptosis and differentiation in human acute myeloid leukemia cells through an AP-1 dependent pathway
GEO Series GSE28185. Homo sapiens. 8 samples. Type: Expression profiling by array.
RNA sequencing of enterocytes differentiated from human iPS cells
GEO Series GSE226851. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans Cell Histiocytosis-like cells
GEO Series GSE114181. Homo sapiens. 26 samples. Type: Expression profiling by array.
Selective requirement of 3D genomic organization for the Mdm1-Il22-Ifng locus regulation in initial Th1 cell lineage specification and differentiation
GEO Series GSE215179. Mus musculus. 30 samples. Type: Expression profiling by high throughput sequencing.
Comparison of skin and in vitro differentiated human Langerhans cells
GEO Series GSE168167. Homo sapiens. 8 samples. Type: Expression profiling by array.
ChIP-seq analysis of Hand1 and Foxf1 at different stages of directed cardiac differentiation of mouse embryonic stem cells
GEO Series GSE245709. Mus musculus. 5 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
LSD1 and CoREST2 Regulate STAT3 Activity to Promote Enteroendocrine Cell Differentiation in Mucinous Colorectal Cancer[CUT&RUN]
GEO Series GSE268956. Homo sapiens. 13 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Regulation of pathogenic T helper 17 cell differentiation by steroid receptor coactivator-3
GEO Series GSE113927. Mus musculus. 8 samples. Type: Expression profiling by array.
LncRNA Mrhl orchestrates differentiation programs in mouse embryonic stem cells through chromatin mediated regulation
GEO Series GSE159757. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.