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Figure 8 from: Maddison DR, Porch N (2021) A preliminary phylogeny and review of the genus Tasmanitachoides, with descriptions of two new species (Coleoptera, Carabidae, Bembidarenini). In: Spence J, Casale A, Assmann T, Liebherr JК, Penev L (Eds) Systematic Zoology and Biodiversity Science: A tribute to Terry Erwin (1940-2020). ZooKeys 1044: 153-196. https://doi.org/10.3897/zookeys.1044.62253
Figure 8 Maximum likelihood trees for COI and 18S. Branch length is shown proportional to relative divergence, as estimated by IQ-TREE; scale bars indicate 0.01 units. Outgroups not depicted.
Figure 9 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 9 Genitalia of Horologion species AH. speokoites median lobe, left lateral aspect BH. speokoites left paramere, left lateral aspect CH. speokoites median lobe, dorsal aspect DH. hubbardi median lobe, left lateral aspect EH. hubbardi left paramere, left lateral aspect FH. hubbardi median lobe, dorsal aspect GH. hubbardi right paramere, right lateral aspect HH. hubbardi female genitalia, ventral aspect A–C after Valentine (1932). Scale bar: 0.1 mm.
Figure 8 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 8 Protarsi of HorologionAH. hubbardi male holotype, dorsal aspect BH. hubbardi female paratype, dorsal aspect CH. speokoites male holotype, dorsal aspect DH. hubbardi male holotype, ventral aspect EH. hubbardi male holotype, dorsal aspect. Scale bars: 0.10 mm.
Figure 3 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 3 Habitat of species of HorologionA surface habitat at Williams Cave, Bath Co., VA B C. Harden (yellow helmet) and T. Malabad (red helmet) in microhabitat of H. hubbardi voucher CWH-452 in Williams Cave C C. Harden examining live H. hubbardi holotype in Williams Cave D live holotype male of H. hubbardi on surface of drip pool in Williams Cave E surface habitat at Arbuckle Cave, Greenbrier Co., WV F T. Malabad examining flowstone in Arbuckle Cave. Photo credit: C. Harden (A, E), K. Kosič Ficco (B–D), M. Miller (F).
Figure 5 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 5 Support for and against our preferred hypothetical placement of Horologion. Black: clade present in maximum likelihood tree, SBS 90% or greater, UFBoot 95% or greater and SH-aLRT 80 or greater. Grey: clade present in maximum likelihood tree, SBS less than 90%, UFBoot less than 95% and/or SH-aLRT less than 80. Red: clade absent in maximum likelihood tree, most-supported contradictory clade with SBS 50% or greater, UFBoot 95% or greater and SH-aLRT 80 or greater. Pink: clade absent in maximum likelihood tree, most-supported contradictory clade with SBS less than 50%, UFBoot less than 95% and/or SH-aLRT less than 80.
Figure 12 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 12 Scanning electron micrographs of the forebody of Horologion hubbardiA head of voucher CWH-452, dorsal aspect, clypeal setae indicated by white arrows B prosternum of non-type specimen, posterior aspect (head, pronotum and most of leg segments removed) C forebody of voucher CWH-452, ventral aspect.
Figure 13 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 13 Distribution map of species of Horologion. Black triangle: Horologion hubbardi, black circle: Horologion speokoites. Grey shaded areas represent exposed karst (Weary and Doctor 2014).
Figure 2 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 2 Horologion hubbardi female paratype, voucher CWH-452 (VMNH), dorsal habitus. Abdomen removed for DNA extraction. Scale bar: 1 mm.
Figure 11 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 11 Elytral characters of Horologion species AH. speokoites holotype, elytra, oblique left lateral aspect BH. hubbardi paratype (CMNH), ditto CH. hubbardi paratype (VMNH), SEM detail of apex of left elytron, ventral aspect DH. hubbardi paratype (VMNH), SEM photo of right elytron, dorsal aspect EH. hubbardi paratype (VMNH), SEM detail of right elytron (area indicated by black box in D). Black arrows: discal setae, white arrows: plica.
Figure 6 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 6 Support for and against previously proposed placements of Horologion. Grey: clade present in maximum likelihood tree, SBS less than 90%, UFBoot less than 95% and/or SH-aLRT less than 80. Red: clade absent in maximum likelihood tree, most-supported contradictory clade with SBS 90% or greater, UFBoot 95% or greater and SH-aLRT 80 or greater. Pink: clade absent in maximum likelihood tree, most-supported contradictory clade with SBS less than 50%, UFBoot less than 95% and/or SH-aLRT less than 80. There were not sufficient sequences available for COI and Topo from our sampled Psydrini.
Figure 14 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 14 Phylogenetic tree of a portion of Trechinae showing proposed synapomorphies of clades. Topology is that of Fig. 4.
Figure 10 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 10 Scanning electron microscope images of Horologion hubbardi female paratype, voucher CWH-452 (VMNH) A detail of right side of pterothorax, ventral aspect B mesoventrite, ventral aspect C metaventrite, ventral aspect, abdomen removed D detail of humeral region of right elytron, dorsal aspect.
Figure 4 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Figure 4 Summary of the maximum likelihood tree of Trechinae from IQ-TREE analysis of 8-gene concatenated matrix. Standard bootstrap support values are shown below nodes. For complete phylogenetic tree showing details within each clade, see Suppl. material 4.
Supplementary material 4 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Trees from Maximum Likelihood and Bootstrap analyses of single gene and 8-gene matrices
Supplementary material 2 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Taxa sampled for phylogenetic analyses
Supplementary material 3 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
Additional photos of Horologion and Bembidarenini
Supplementary material 1 from: Harden CW, Davidson RL, Malabad TE, Caterino MS, Maddison DR (2024) Phylogenetic systematics of the enigmatic genus Horologion Valentine, 1932 (Coleoptera, Carabidae, Trechinae, Horologionini), with description of a new species from Bath County, Virginia. Subterranean Biology 48: 1-49. https://doi.org/10.3897/subtbiol.48.114404
PCR primers and protocols
Figure 7 from: Sivayyapram V, Kunsete C, Xu X, Smith DR, Traiyasut P, Deowanish S, Aung MM, Ono H, Li D, Warrit N (2024) Seven new species of the segmented spider genus Liphistius (Mesothelae, Liphistiidae) in Thailand and Myanmar. ZooKeys 1189: 203-229. https://doi.org/10.3897/zookeys.1189.115850
Figure 7 Liphistius kaengkhoi sp. nov. male palp and vulva plate A–D ARA-2018-284 (holotype) palp A prolateral view B ventral view C retrolateral view D distal view E, F ARA-2018-286 (allotype) vulva plate E ventral view F dorsal view. Abbreviations: CDO = central dorsal opening; CT = contrategulum; Cu = cumulus; de = distal edge of the contrategulum; Em = embolus; GA = genital atrium; mm = millimeter; PC = paracymbium; PeP = paraembolic plate; PP = poreplate; PS = posterior stalk; RC = receptacular cluster; ST = subtegulum; T = tegulum; TiA = tibial apophysis. Scale bar: 1 mm.
Figure 8 from: Sivayyapram V, Kunsete C, Xu X, Smith DR, Traiyasut P, Deowanish S, Aung MM, Ono H, Li D, Warrit N (2024) Seven new species of the segmented spider genus Liphistius (Mesothelae, Liphistiidae) in Thailand and Myanmar. ZooKeys 1189: 203-229. https://doi.org/10.3897/zookeys.1189.115850
Figure 8 Liphistius hintung sp. nov. A, B male ARA-2018-299 (holotype) C, D female, ARA-2018-296 (allotype) A, C dorsal view B, D lateral view. Scale bar: 10 mm.
Figure 13 from: Sivayyapram V, Kunsete C, Xu X, Smith DR, Traiyasut P, Deowanish S, Aung MM, Ono H, Li D, Warrit N (2024) Seven new species of the segmented spider genus Liphistius (Mesothelae, Liphistiidae) in Thailand and Myanmar. ZooKeys 1189: 203-229. https://doi.org/10.3897/zookeys.1189.115850
Figure 13 Liphistius champakpheaw sp. nov. male palp and vulva plate A–D ARA-2017-146 (holotype) palp A prolateral view B ventral view C retrolateral view D distal view E, F ARA-2018-146a (allotype) vulva plate E ventral view F dorsal view. Abbreviations: CDO = central dorsal opening; CT = contrategulum; Cu = cumulus; de = distal edge of the contrategulum; Em = embolus; GA = genital atrium; mm = millimeter; PC = paracymbium; PeP = paraembolic plate; PP = poreplate; PS = posterior stalk; RC = receptacular cluster; ST = subtegulum; T = tegulum; TiA = tibial apophysis. Scale bar: 1 mm.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.