Find research datasets worth reusing

Supplementary material 8 from: Jeunen G-J, Lipinskaya T, Gajduchenko H, Golovenchik V, Moroz M, Rizevsky V, Semenchenko V, Gemmell NJ (2022) Environmental DNA (eDNA) metabarcoding surveys show evidence of non-indigenous freshwater species invasion to new parts of Eastern Europe. Metabarcoding and Metagenomics 6: e68575. https://doi.org/10.3897/mbmg.6.e68575

Environmental DNA detections from the passive surveillance for both metabarcoding assays

Supplementary material 2 from: Jeunen G-J, Lipinskaya T, Gajduchenko H, Golovenchik V, Moroz M, Rizevsky V, Semenchenko V, Gemmell NJ (2022) Environmental DNA (eDNA) metabarcoding surveys show evidence of non-indigenous freshwater species invasion to new parts of Eastern Europe. Metabarcoding and Metagenomics 6: e68575. https://doi.org/10.3897/mbmg.6.e68575

Metabarcoding qPCR assays and the respective primer sets used for biodiversity detection

Supplementary material 5 from: Jeunen G-J, Lipinskaya T, Gajduchenko H, Golovenchik V, Moroz M, Rizevsky V, Semenchenko V, Gemmell NJ (2022) Environmental DNA (eDNA) metabarcoding surveys show evidence of non-indigenous freshwater species invasion to new parts of Eastern Europe. Metabarcoding and Metagenomics 6: e68575. https://doi.org/10.3897/mbmg.6.e68575

Relative fish abundances as observed by the ichthyological survey

Supplementary material 2 from: Naro-Maciel E, Ingala MR, Werner IE, Reid BN, Fitzgerald AM (2022) COI amplicon sequence data of environmental DNA collected from the Bronx River Estuary, New York City. Metabarcoding and Metagenomics 6: e80139. https://doi.org/10.3897/mbmg.6.80139

Tables S1,S2, Figures S1–S3

Supplementary material 4 from: Jeunen G-J, Lipinskaya T, Gajduchenko H, Golovenchik V, Moroz M, Rizevsky V, Semenchenko V, Gemmell NJ (2022) Environmental DNA (eDNA) metabarcoding surveys show evidence of non-indigenous freshwater species invasion to new parts of Eastern Europe. Metabarcoding and Metagenomics 6: e68575. https://doi.org/10.3897/mbmg.6.e68575

Bioinformatic and statistical scripts used to process eDNA data

Supplementary material 1 from: Naro-Maciel E, Ingala MR, Werner IE, Reid BN, Fitzgerald AM (2022) COI amplicon sequence data of environmental DNA collected from the Bronx River Estuary, New York City. Metabarcoding and Metagenomics 6: e80139. https://doi.org/10.3897/mbmg.6.80139

Supplementary Data Files 1, 2

Supplementary material 1 from: Jeunen G-J, Lipinskaya T, Gajduchenko H, Golovenchik V, Moroz M, Rizevsky V, Semenchenko V, Gemmell NJ (2022) Environmental DNA (eDNA) metabarcoding surveys show evidence of non-indigenous freshwater species invasion to new parts of Eastern Europe. Metabarcoding and Metagenomics 6: e68575. https://doi.org/10.3897/mbmg.6.e68575

Sampling sites and their description

Supplementary material 7 from: Jeunen G-J, Lipinskaya T, Gajduchenko H, Golovenchik V, Moroz M, Rizevsky V, Semenchenko V, Gemmell NJ (2022) Environmental DNA (eDNA) metabarcoding surveys show evidence of non-indigenous freshwater species invasion to new parts of Eastern Europe. Metabarcoding and Metagenomics 6: e68575. https://doi.org/10.3897/mbmg.6.e68575

Rarefaction curves for each metabarcoding assay

Supplementary material 1 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

Community types

Supplementary material 6 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

16S MOTU table

Supplementary material 7 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

Tables and figures

Supplementary material 5 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

COI MOTU table

Supplementary material 3 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

16S reference library

Supplementary material 8 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

Lysis buffers and purification protocols

Supplementary material 4 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

Bioinformatic pipeline

Supplementary material 2 from: Marquina D, Roslin T, Łukasik P, Ronquist F (2022) Evaluation of non-destructive DNA extraction protocols for insect metabarcoding: gentler and shorter is better. Metabarcoding and Metagenomics 6: e78871. https://doi.org/10.3897/mbmg.6.78871

COI reference library

Supplementary material 2 from: Jiménez Elvira N, Ushio M, Sakai S (2022) Are microbes growing on flowers evil? Effects of old flower microbes on fruit set in a wild ginger with one-day flowers, Alpinia japonica (Zingiberaceae). Metabarcoding and Metagenomics 6: e84331. https://doi.org/10.3897/mbmg.6.84331

Fruit and seed set datasets

Supplementary material 1 from: Blancher P, Lefrançois E, Rimet F, Vasselon V, Argillier C, Arle J, Beja P, Boets P, Boughaba J, Chauvin C, Deacon M, Duncan W, Ejdung G, Erba S, Ferrari B, Fischer H, Hänfling B, Haldin M, Hering D, Hette-Tronquart N, Hiley A, Järvinen M, Jeannot B, Kahlert M, Kelly M, Kleinteich J, Koyuncuoğlu S, Krenek S, Langhein-Winther S, Leese F, Mann D, Marcel R, Marcheggiani S, Meissner K, Mergen P, Monnier O, Narendja F, Neu D, Onofre Pinto V, Pawlowska A, Pawlowski J, Petersen M, Poikane S, Pont D, Renevier M-S, Sandoy S, Svensson J, Trobajo R, Tünde Zagyva A, Tziortzis I, van der Hoorn B, Vasquez MI, Walsh K, Weigand A, Bouchez A (2022) A strategy for successful integration of DNA-based methods in aquatic monitoring. Metabarcoding and Metagenomics 6: e85652. https://doi.org/10.3897/mbmg.6.85652

Table S1

Supplementary material 1 from: Jiménez Elvira N, Ushio M, Sakai S (2022) Are microbes growing on flowers evil? Effects of old flower microbes on fruit set in a wild ginger with one-day flowers, Alpinia japonica (Zingiberaceae). Metabarcoding and Metagenomics 6: e84331. https://doi.org/10.3897/mbmg.6.84331

Tables S1, S2 and Figures S1–S4

Supplementary material 2 from: Swenson SJ, Eichler L, Hörren T, Kolter A, Köthe S, Lehmann GUC, Meinel G, Mühlethaler R, Sorg M, Gemeinholzer B (2022) The potential of metabarcoding plant components of Malaise trap samples to enhance knowledge of plant-insect interactions. Metabarcoding and Metagenomics 6: e85213. https://doi.org/10.3897/mbmg.6.85213

Table S2