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2,097 results for “2001”
Microbial Sampling from Shark River Slough and Taylor Slough, Everglades National Park, South Florida, USA (FCE LTER), January 2001 - December 2023
We collected monthly observations of water microbial characteristics from each of the FCE LTER sites beginning in February 2001. Bacteria counts using DAPI epifluorescence, heterotrophic bacteria production with tritiated Thymidine uptake, and algal energetics and chl a using the PAM fluorometer were determined from each of the water samples. Bacteria counts in Shark River Slough in the freshwater end of the transect revealed highest bacteria numbers upon marsh rewetting after the dry down. The highest overall count of bacteria was found at the most freshwater point adjacent to the perimeter of the water conservation area but only in March of 2001. Peak numbers of bacteria in the saline end of Shark River transect occurred in December and July. There is some evidence that these higher bacteria numbers in the SR transect are associated with higher salinities. Highest bacteria counts along the Taylor slough transect were observed at the mouth of the Taylor River with peak counts in the wet season in October. Bacteria densities also increased from the eastern to western end of the transect in Florida Bay. Heterotrophic bacterial production did not relate closely bacteria numbers and exhibited highest numbers in the dry season. There was no discernible relationship between salinity and bacteria production as seen in bacteria numbers. Monthly observations using PAM fluorometry revealed that brown algae was the greatest contribution to the algal chal a pool and this relationship was consistent across all FCE LTER sites. Converse to the pattern of bacteria abundance along the Shark River transect, there was evidence that algal biomass decreases from freshwater to estuary. In the Shark River, algal energetics increased from marsh to estuary as well as in the panhandle region of the Taylor River transect. From these observations we conclude the behavior of the microbial loop and interactions generated between functional guilds is highly variable along individual transects an
Water Quality Data (Porewater) from the Shark River Slough, Everglades National Park (FCE LTER), Florida, USA, January 2001 - ongoing
Porewater samples are collected at Florida Coastal Everglades Long Term Ecological Research (FCE LTER) Program Shark River Slough sites SRS1b (not active), SRS1c (not active), SRS1d, SRS2, and SRS3 within the 1 m^2 aboveground sawgrass nondestructive biomass plots. Airstones connected to Tygon tubing are inserted 10-15 cm below the soil surface. Biannually during the wet season, Monoject 140cc syringes are used to collect at least 80 mL of porewater sample and after collection they are immediately placed in an ice filled cooler. The lab processing of these samples include salinity measurements using a YSI, and filtering the sample through a filter (Whatman GF/F from 2000 to 2017 and Whatman polydisc GW from 2017 to present) . Samples are analyzed for inorganic nutrients such as NO2-,NO3-,NH4+,SRP, and DOC. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques and DOC is quantified on a Shimadzu TOC Analyzer.
Water Quality Data (Grab Samples) from the Shark River Slough, Everglades National Park (FCE LTER), Florida, USA, May 2001 - ongoing
Water quality samples are being collected using ISCO autosamplers at all Florida Coastal Everglades Long Term Ecological Research (FCE LTER) Program wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a filter (Whatman GF/F from 2000 to 2017 and Whatman polydisc GW from 2017 to ongoing) immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP and TN. We use these monthly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an ANTEK 9000N analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with a YSI conductivity meter or refractometer. In January 2007 a 6 days interval began for SRS1d, SRS2 & SRS3; the ISCOs did not change their sampling scheduled, they kept collecting water sample every 18 hours and switching bottles every three days; once in the lab, the 3 days was turned into 6 days by combining two bottles into 2 liters container. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rai
Water Quality Data (Grab Samples) from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, May 2001 - ongoing
Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these montly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with a YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.
Monthly monitoring fluorescence data for Florida Bay, Ten Thousand Islands, and Whitewater Bay, in southwest coast of Everglades National Park (FCE) for February 2001 to December 2002
Dissolved organic matter plays an important role in biogeochemical processes in aquatic environments such as elemental cycling, microbial loop energetics, and the transport of materials across landscapes. Since most of N (Greater than 90%) and P (around 90%) is in the organic form in the oligotrophic subtropical Florida Coastal Estuaries (FCES), study of the source and dynamics of dissolved organic matter (DOM) in the ecosystem is crucial for the better understanding of the biogeochemical cycling of nutrients. FCES are composed of estuaries with distinct regions with different biogeochemical processes. Florida Bay (FB) is a wedge-shaped shallow oligotrophic estuary which lays south of the Everglades, the bottom of which is covered with a dense biomass of seagrass. Whitewater bay (WWB) is a semi-enclosed mangrove estuary with a relatively long residence time, which receives overland freshwater input from the Everglades marshes. Ten thousand Islands (TTI) covers the southwest margin of the Florida Coastal Everglades, which are highly compartmentalized by local geomorphology. The sources of both freshwater and nutrients in FCES are difficult to quantify, owing to the non-point source nature of runoff from the Everglades and the dendritic cross channels in the mangroves. Furthermore, the combination of multiple DOM sources (freshwater marsh vegetation, mangroves, phytoplankton, seagrass, etc.), and the potential seasonal variability of their relative contribution, along with the history of (photo)chemical and microbial diagenetic processing, and complex advective circulation, makes the study of DOM dynamics in FCES particularly difficult using standard schemes of estuarine ecology. Quantitative information of DOM is very useful to investigate the biogeochemical cycling of DOM to a certain degree, however, qualitative information is necessary to better understand the source and dynamics of DOM. Since fluorescence spectroscopic techniques are very sensitive, quick and sim
Chemical characteristics of dissolved organic matter in an oligotrophic subtropical wetland/estuary ecosystem, Everglades National Park (FCE), South Florida from December 2001 to January 2002
The objective of this study was to investigate in detail the molecular characteristics of DOM in a sub-tropical, oligotrophic aquatic environment, namely the Florida coastal Everglades, along a freshwater wetland, mangrove fringe to estuarine transect. For this purpose, surface waters from five sites along Taylor Slough ranging from freshwater marsh to mangrove ecotone environments, as well as three sites within the Florida Bay estuary were collected for analyses. Fluorescence properties of DOM were measured for a quick assessment of DOM quality. Ultrafiltered DOM samples (UDOM; less than 0.7 um, greater than 1000 Da) were concentrated and freeze dried for the determination of bulk C composition, lignin-phenol concentration, and neutral sugar composition. These molecular characteristics were investigated by 13C-NMR spectroscopy, TMAH thermochemolysis, and hydrolysis with trifluoroacetic acid (TFA), respectively. Furthermore, UDOM samples were classified by cluster analysis based on their pyrolysis products obtained via flash pyrolysis-GC/MS.
Characterization of dissloved organic nitrogen in an oligotrophic subtropical coastal ecosystem (Taylor Slough and Shark River Slough) for December 2001 in Everglades National Park (FCE), South Florida, USA
A better understanding of the biogeochemical cycling of nutrients entering Florida Bay is a key issue regarding the restoration of the Everglades. In addition to precipitation, the other major source of freshwater to Florida Bay is from Taylor Slough and the C-111 Basin in the northeast section of the Bay. While it is known that these areas deliver significant amounts of N to the Bay, a significant portion of this is in the form of dissolved organic N (DON). The sources, environmental fate and bioavailability to microorganisms of this DON are however, not known. Should this DON be readily available, any increased load as a function of restoration changes might have an impact on internal phytoplankton bloom dynamics. No significant flocculation or precipitation of DOM occurred with increase in salinity, meaning that terrestrial DOM does not get trapped in the sediments but stays in the water column where it subjected to photolysis and advective transport. Sunlight has a significant effect on the chemical characteristics of DOM. While the DOC levels did not change significantly during photo-exposure, the optical characteristics of the DOM were modified. The environmental implications of this are conflicting: photo-induced polymerization may stabilize the DOM by reducing its bioavailability while photolysis may make the DOM more labile. Overall, DON bioavailability was relatively low in this region. Even though the amount of DON loaded to the bay may be significant, the fraction of DON available for microbial cycling is much smaller. The amount of N supplied by recycling may be a significant portion of the total DIN pool. All this must be considered in context with the proposed CERP modifications to flows. As of the latest initial Comprehensive Everglades Restoration Project (CERP) update, the flows to Taylor Slough and C-111/Panhandle Basis are not predicted to change very much from base conditions. Therefore we do not expect any great increases in TN loading in this
Florida Bay Physical Data, Everglades National Park (FCE), South Florida from January 2001 to February 2002
Florida Bay physical data that includes surface temperature and salinity at Duck Key, Bob Allen Keys, and Sprigger Bank, Florida Bay in Everglades National Park, South Florida.
Radiation measurements at Key Largo Ranger Station, South Florida (FCE) for July 2001
Basic radiation data, including Infra-red canopy temperatures, collected as 1 minute averages from a 5 m tower at Key Largo Ranger Station, South Florida, near Everglades National Park.
Meteorological measurements at Key Largo Ranger Station, South Florida (FCE) for July 2001 to August 2001
Basic radiation data, including Infra-red canopy temperatures, collected as 1 minute averages from a 5 m tower at Key Largo Ranger Station, South Florida, near Everglades National Park.
Mangrove leaf physiological response to local climate at Key Largo, Watson River Chickee, Taylor Slough, and Little Rabbit Key, South Florida (FCE) from July 2001 to August 2001
Determine the red mangrove leaf physiological response to the local climate to understand the local controls on plant physiology. Data were collected in the Key Largo Ranger Station, Watson River Chickee and Taylor Slough research Sites, South Florida.
Rubisco limited photosynthesis rates of Red mangrove leaves at Key Largo, Watson River Chickee, Taylor Slough, and Little Rabbit Key, South Florida (FCE) from July 2001 to August 2001
Determine the Rubisco limited carboxylation rates of red mangrove ( species Rhizophora mangle) leaves. This information will be used to model carbon sequestration by Red mangroves.
Light limited carboxylation rates of Red mangrove leaves at Key Largo, Watson River Chickee, Taylor Slough, and Little Rabbit Key, South Florida (FCE) from July 2001 to August 2001
Our goal is to determine light limited carboxylation rates of red mangrove (specie sRhizophora mangle) leaves. This information will be used to model carbon sequestration by Red mangroves.
Gastropod Biomass and Densities found at Rabbit Key Basin, Florida Bay (FCE) from March 2000 to April 2001
Grazing gastropod biomass and shell morphology were measured from 1 m2 plots within Thalassia seagrass meadow in Rabbit Key Basin, Florida Bay, Everglades National Park.
Seagrass Epiphyte Accumulation for Florida Bay, South Florida (FCE) from December 2000 to September 2001
Total epiphyte and epiphyte chlorophyll-a loads were measured from artificial seagrass leaves (Mylar) deployed for periods up to the approximate Thalassia leaf turnover period.
Mean Seagrass Epiphyte Accumulation for Florida Bay, South Florida (FCE) from December 2000 to September 2001
Total epiphyte and epiphyte chlorophyll-a loads were measured from artificial seagrass leaves (Mylar) deployed for periods up to the approximate Thalassia leaf turnover period. Data were collected in Florida Bay, within Everglades National Park.
Seagrass Epiphyte Accumulation: Epiphyte Loads on Thalassia testudinum in Rabbit Key Basin, Florida Bay (FCE) from March 2000 to April 2001
Total epiphyte and epiphyte chlorophyll-a loads and leaf nutrients were measured on Thalassia seagrass short-shoots from Rabbit Key Basin, Florida Bay.
Thalassia leaf morphology and productivity measurements from arbitrary plots located in a Thalassia seagrass meadow in Rabbit Key Basin, Florida Bay (FCE) from March 2000 to April 2001
Thalassia leaf morphology and productivity were measured from six arbitrary 200 cm2 plots within a Thalassia seagrass meadow in Rabbit Key Basin, Florida Bay.
Greenhouse experiment (FCE) in April and August 2001: Responses of neotropical mangrove saplings to the combined effect of hydroperiod and salinity/Biomass
A greenhouse experiment was performed for 13.5 months to evaluate the effect of salinity and hydroperiod on seedling growth rates of 2 mangrove species( Laguncularia racemosa and Rizhophora mangle). Data analyses are currently being performed.
Bulk Parameters for Soils/Sediments from the Shark River Slough and Taylor Slough, Everglades National Park (FCE), from October 2000 to January 2001
The main goal of this research is the characterization of the sources, transport and fate of soil/sediment organic matter in Everglades National Park, and the determination of future potential effects of Everglades restoration on the source strength and accumulation rates of this OM. The general approach of this study is the seasonal sampling and analysis of bulk and molecular characteristics of soil/sediment samples from the FCE-LTER sites. Bulk parameters measured are d13C, C/N and %OC, while molecular characterizations are based on detailed biomarker analyses of extractable lipids and CP/MAS 13C-NMR of bulk OM. Preliminary results have allowed to characterize OM source strength throughout the transects, as well as determining other minor OM sources, diagenetic differences based upon OM quality and marine influences in the estuarine areas of the FCE-LTER study area.
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