Find research datasets worth reusing

opencc-by-4.0Dec 2019View details →

Fig. 1 in Detection and quantification of house mouse Eimeria at the species level - Challenges and solutions for the assessment of coccidia in wildlife

Fig. 1. Geographical localization of house mice (Mus musculus) collected for this study and comparison of diagnostic methods for Eimeria. A) Localization from the 378 mice included in the present study, colors indicate the Eimeria species identified for each. B) Venn diagram showing the overlap between detection methods and successful genotyping identification of the isolates. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

opencc-by-4.0Dec 2019View details →

Fig. 3 in Detection and quantification of house mouse Eimeria at the species level - Challenges and solutions for the assessment of coccidia in wildlife

Fig. 3. Morphological and morphometrical characteristics of Eimeria oocyst isolated from Mus musculus. a) Photomicrographs at 1000x amplification of Eimeria oocyst from the three species isolated from Mus musculus (red = E. falciformis; green = E. ferrisi and yellow = E. vermiformis). Length/Width ratio from b) oocyst and c) sporocysts corresponding to each species (E. falciformis n = 31; E. ferrisi n = 127 and E. vermiformis n = 35). Mean ± 95% Confidence Interval is plotted. * Represent significant difference (Tukey HSD, p <0.05). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2. The PCR products identified within the 18S in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation

Fig. 2. The PCR products identified within the 18S rRNA of Eimeria bovis following digestion with two restriction endonucleases: AluI recognising AG∧CT and Hin1II recognising CATG∧. M1: GeneRuler 100 bp Plus DNA Ladder (Thermo Fisher Scientific); M2: GeneRuler 50bp DNA Ladder (Thermo Fisher Scientific); lane 1: European bison colon wall tissue; lane 2: European bison colon wall tissue after digestion; lane 3: E. bovis oocysts of European bison; lane 4: E. bovis oocysts of European bison after digestion.

Fig. 3 in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation

Fig. 3. The virtual double digestion of the 18S rRNA gene of eimerians infecting the large intestine of the European bison with the restriction enzymes Mval (BstNI) recognising CC∧WGG, and KpnI recognising GGTAC∧C, simulated with SnapGene version 5.0.6 (GSL Biotech LLC); M: GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific). (A) A three-band pattern for E. bovis (20 bp, 210 bp, 343 bp). (B) A four-band pattern for E, zuernii (20 bp, 100 bp, 210 bp, 242 bp). (C) A two-band pattern for E. alabamensis (212 bp, 362 bp).

Fig. 1 in Usefulness of PCR-RFLP of 18S rRNA gene for rapid post-mortem diagnostics of highly pathogenic Eimeria spp. (Apicomplexa: Eimeriidae) of European bison, Bison bonasus L. with histopathological correlation

Fig. 1. Histopathological lesions associated with endogenous stages of Eimeria spp. in sections of the ileum and colon of European bison (H-E staining). (A) Shortening and blunting of the intestinal villi of the ileum with diffuse infiltration of mononuclear inflammatory cells within the lamina propria, edematous stroma, dilated crypt containing necrotic debris (arrow), and atrophy of submucosal lymphoid follicles (× 20 magnification). (B) Schizonts and degenerating merozoites in the crypt lumen of the colon (arrows); immature macrogamont with a central nucleus (arrowhead) (× 1000 magnification). (C) Immature microgamonts in the epithelial cells of the colon crypt (arrows) (× 400 magnification). (D) Mature microgamont in the epithelial cells of the colon crypt (arrow) (× 1000 magnification). (E) Gametogonic stages of Eimeria development in the epithelial cells of the colon. Microgamont with peripheral microgames (arrowhead), (a) nearly mature microgamonts, (b) macrogamont with eosinophilic wall-forming bodies, (c) early oocyst (× 400 magnification). (F) Mature macrogamont in the epithelial cells of the cecum (arrow) (× 1000 magnification).

Fig. 5 in Parasitic development in intestines and oocyst shedding patterns for infection by Eimeria uekii and Eimeria raichoi in Japanese rock ptarmigans, Lagopus muta japonica, protected by cages in the Southern Japanese Alps

Fig. 5. Eimeria oocysts (E. uekii) isolated from soil inside the cage (cage No. 6) (A) on Mt. Kita (35̊40′N, 138̊14′E), one of their habitats in the southern Japanese Alps and on Mt. Norikura as other habitats on northern Japanese Alps (B). In Fig. A, the sporocysts and sporozoites are clearly formed.

Fig. 4 in Parasitic development in intestines and oocyst shedding patterns for infection by Eimeria uekii and Eimeria raichoi in Japanese rock ptarmigans, Lagopus muta japonica, protected by cages in the Southern Japanese Alps

Fig. 4. Number of oocysts per gram (OPG) as seasonal detection rate for E. raichoi of hens (solid bars) and chicks (open bars) in cage Nos. 4–6 in 2019. Double arrows show the periods during which feces of hens were examined. ND indicates that we could not collect feces and did not determine the OPG.

Fig. 2 in Parasitic development in intestines and oocyst shedding patterns for infection by Eimeria uekii and Eimeria raichoi in Japanese rock ptarmigans, Lagopus muta japonica, protected by cages in the Southern Japanese Alps

Fig. 2. Histopathological photomicrograph of a section of the intestines of dead chicks during cage protection in 2018. Figs. A and B show developmental trophozoites (arrows) and schizonts (arrows) of Eimeria spp. at the epithelial cells of the colon (Chick c) and ileum (Chick a), respectively. Some zoites (arrows) invade into submucosa (ileum of Chick b) (Fig. C). Figs. D, E, and F show the sexual zoites or cavities after releasing oocysts (arrows) of the ileum (Chick a), ceca (Chick d), and ileum (Chick c). Arrowheads in Fig. F indicate hemorrhages in the intestinal mucosa.

Fig. 1 in Parasitic development in intestines and oocyst shedding patterns for infection by Eimeria uekii and Eimeria raichoi in Japanese rock ptarmigans, Lagopus muta japonica, protected by cages in the Southern Japanese Alps

Fig. 1. Shelter used for cage protection of Japanese rock ptarmigan broods on Mt. Kita (35̊40′N, 138̊14′E), Japan in 2019.

opencc-by-4.0Aug 2021View details →

Fig. 3. Sporulation rates for E in Distribution of Eimeria uekii and Eimeria raichoi in cage protection environments for the conservation of Japanese rock ptarmigans (Lagopus muta japonica) in the Japanese Alps

Fig. 3. Sporulation rates for E. uekii (A) and E. raichoi (B) at temperatures of 15, 20, and 25 ◦ C.

opencc-by-4.0Aug 2021View details →

Fig. 1 in Distribution of Eimeria uekii and Eimeria raichoi in cage protection environments for the conservation of Japanese rock ptarmigans (Lagopus muta japonica) in the Japanese Alps

Fig. 1. Location of Mt. Norikuradake (36◦06′N, 137◦33′E) in the Northern Japanese Alps (1) and Mt. Komagatake (35◦79′N, 137◦80′E) in the Central Japanese Alps (2). Three broods were transported from Mt. Norikuradake to Mt. Komagatake.

opencc-by-4.0Aug 2021View details →

Fig. 2 in Distribution of Eimeria uekii and Eimeria raichoi in cage protection environments for the conservation of Japanese rock ptarmigans (Lagopus muta japonica) in the Japanese Alps

Fig. 2. Internal and external appearance of a shelter cage used for cage protection of Japanese rock ptarmigan broods on Mt. Norikuradake (A and B, respectively) with location numbers to indicate the soil sample collection positions within the cages (C). OPG values for the soil samples within the cages at Mt. Norikuradake (D; E. uekii and E; E. raichoi) and Mt. Komagatake (F; E. uekii and G; E. raichoi).

Fig. 2 in Experimental evaluation of pathogenicity and acquired immunity of Eimeria species, E. uekii and E. raichoi, infecting Japanese rock ptarmigans in a subspecies of the birds

Fig. 2. The number of oocysts per gram of feces (OPG) after inoculation with E. uekii and E. raichoi oocysts. Panels A and B show E. uekii and E. raichoi OPG, respectively. Chicks Nos. 1–3 were inoculated depending on the manner of oocysts E. uekii and E. raichoi as described at the bottom of Figure, and mouse no. 4 was inoculated with oocysts of E. raichoi.

Fig. 1 in Experimental evaluation of pathogenicity and acquired immunity of Eimeria species, E. uekii and E. raichoi, infecting Japanese rock ptarmigans in a subspecies of the birds

Fig. 1. Weight gain of Svalbard rock ptarmigans after inoculation with E. uekii and E. raichoi oocysts. The inoculation doses of E. uekii and E. raichoi were 4 104 and × 17 103 or 4 103 (A), 2 104 and 8 103 (B), 4 103 and 17 or 4 102 (C), and 4 102 and 17 or 4 101 (D), respectively. Group E was administered PBS as a × × × × × × × × control, and group F was inoculated with 4 104 oocysts of E. raichoi that had been passed in turkeys. "n" indicates the numbers of examined chicks.

Fig. 5 in Experimental evaluation of pathogenicity and acquired immunity of Eimeria species, E. uekii and E. raichoi, infecting Japanese rock ptarmigans in a subspecies of the birds

Fig. 5. Histopathological photomicrographs of sections of the intestines of challenged chicks. Panels A and B show the ileum and duodenum of the primary inoculated chick at 7 days PI, respectively. Panels C and D show the ileum and colon of a non-inoculated chick at 7 days PI, respectively. Arrows indicate developmental zoites (sexual stages in panels C and D). Scale bars are 20 μm in panels A and C and 50 μm in panels B and D.

Fig. 4 in Experimental evaluation of pathogenicity and acquired immunity of Eimeria species, E. uekii and E. raichoi, infecting Japanese rock ptarmigans in a subspecies of the birds

Fig. 4. Weight gain of Svalbard rock ptarmigans after challenge inoculation. Panels A and B show the weight of control (PBS) and primary inoculated chicks, respectively. Two-direction arrows indicate the period of challenge inoculation, 4 104 E. uekii and 2 103 E. raichoi for 5 days.

Fig. 3 in Experimental evaluation of pathogenicity and acquired immunity of Eimeria species, E. uekii and E. raichoi, infecting Japanese rock ptarmigans in a subspecies of the birds

Fig. 3. Histopathological photomicrographs of sections from an experimentally inoculated chick (Svalbard rock ptarmigan). Panel A shows the ileum of the chick at 4 days PI after inoculation with 4 × 104 E. uekii and 2 × 103 E. raichoi oocysts. Panel B is a higher magnification of the section. Arrows indicate developmental zoites or mature schizonts (in panel B). Scale bars in panels A and B are 20 μm and 50 μm, respectively.

Fig. 2 in Eimeria erythrorhynchosi n. sp. (Apicomplexa: Eimeriidae) from the American white pelican Pelecanus erythrorhynchos Gmelin, 1789 (Pelecaniformes: Pelecanidae) in Toluca, Mexico

Fig. 2. Line drawing of a sporulated oocyst of Eimeria erythrorhynchosi n. sp. from Pelecanus erythrorhynchos. Scale-bar: 10 μm.

Fig. 1 in Eimeria erythrorhynchosi n. sp. (Apicomplexa: Eimeriidae) from the American white pelican Pelecanus erythrorhynchos Gmelin, 1789 (Pelecaniformes: Pelecanidae) in Toluca, Mexico

Fig. 1. Photomicrographs of sporulated oocysts and sporocysts of Eimeria erythrorhynchosi n. sp. A, Ovoidal oocyst with clearly visible ovoidal sporocysts with a polar granule (PG) beneath the c. B, A sporocysts with clearly flattened Stieda body (SB) and rounded sub-Stieda body (SSB); C, Ovoidal oocyst with clearly visible ovoidal sporocysts with a polar granule (PG) beneath the micropyle in outer layer (MOL); D, One oocyst showing micropyle in outer layer (MOL) and sporocyst residuum (SR), consisting of many spherules. Scale-bars: 10 μm.