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135 results for “Isolation by resistance”
Specific monitoring results of ESBL-/AmpC-/carbapenemase-producing bacteria and specific monitoring of carbapenemase-producing bacteria, in the absence of isolates detected complementing the European Union Overview Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2017/2018
<p>This dataset derives from the specific monitoring of E. coli producers of ESBLs/AmpC/carbapenemases, as well as the specific monitoring of carbapenemase-producers (voluntary reporting), in the absence of any isolates detected. This dataset contains only data when Total units positive equals zero '0'.</p>
Data from: Antibacterial activity of graphene oxide nanosheet against multi drug resistant superbugs isolated from infected patients
Graphene oxide (GO) is a derivative of graphene nanosheet which is the most promising material of the decade in biomedical research. In particular, it has been known as an antimicrobial nanomaterial with good biocompatibility. In this study, we have synthesized and characterize GO and checked its antimicrobial property against different Gram-negative and Gram-positive multi drug resistant (MDR) hospital superbugs grown in solid agar-based nutrient plates with and without human serum through the utilization of agar well diffusion method, live/dead fluorescent staining and genotoxicity analysis. The main focus of the antimicrobial activity analysis is to distinguish the killing performance of GO in artificial and blood containing media because after injection into the bloodstream the activity of GO may be modified by adsorption of blood proteins or other biomolecules. No significant changes in antibacterial activity were found in these two different conditions. We also compare the bactericidal capability of GO with some commonly administrated antibiotics and in all cases the degree of inhibition is found to be higher. The data presented here are novel and show that GO is an effective bactericidal agents against different superbugs and can be used as a future antibacterial agent.
Draft genomes of 972 carbapenem-resistant Pseudomonas aeruginosa isolates (shovill assemblies of Reyes et al 2023 dataset raw reads)
<p>This dataset contains shovill assemblies of raw reads released under NCBI BioProject PRJNA824880 generated in Reyes J et al (The Lancet Microbe. Volume 4 Issue 3 Pages e159-e170 (March 2023); DOI: 10.1016/S2666-5247(22)00329-9)</p> <p> </p>
Direct prediction for carbapenemase-producing and colistin-resistant Klebsiella pneumoniae isolates from routine MALDI-TOF mass spectrum using machine learning
<p>The emergence of carbapenem-nonsusceptible K. pneumoniae (CnSKP) leads a serious threat to patient survival and colistin resistance makes the treatment of CnSKP more difficultly. To make treatment strategy properly and quickly, we aimed to develop a rapid prediction method for CnSKP and colistin-resistant K. pneumoniae (ColRKP) based on the spectra of routine matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI–TOF MS). The machine learning (ML) model for differentiating CnSKP and carbapenem-susceptible K. pneumoniae (CSKP) showed accuracy of 0.8869 and AUC of 0.9551; the model for ColRKP and colistin-intermediate K. pneumoniae (ColIKP) showed accuracy of 0.8361 and the AUC of 0.8447.</p>
Phenotypic and genotypic analysis of drug resistance in M. tuberculosis isolates in Gansu, China
<p>Tuberculosis has posed a serious threat to human health. It is imperative to investigate the geographic prevalence of tuberculosis and medication resistance, as this information is essential for informing strategies for its prevention and treatment. Drug resistance was identified using a proportion method. Drug-resistant genes and pathways were predicted using whole genome sequencing. The drug resistance range of bedaquiline was identified using the microporous plate two-fold dilution method, and drug resistance genes were studied using sequencing. The study revealed that 19.99% of the tuberculosis cases had multidrug resistance. The genes of<em> M. tuberculosis</em> are predominantly involved in the synthesis of ABC transporters, two-component systems, and bacterial secretion systems, as well as in energy production and conversion, and lipid transport and metabolism. The genes encode for 82.45% of carbohydrate-related enzymes such as glycoside hydrolases, glycosyl transferases, and carbohydrate esterases. The minimum inhibitory concentration (MIC) of bedaquiline against clinical strains was approximately 0.06 μg/mL, with identified mutations in drug-resistant genes Rv0678, atpE, and pepQ, specifically V152A, P62A, and T222N, respectively. The multidrug resistance tuberculosis development was attributed to the strong medication resistance exhibited. It was concluded that tuberculosis had presented a high level of drug resistance. Phenotypic resistance was related to genes, existing potential genetic resistance in <em>M. tuberculosis</em>. Bedaquiline was found to possess effective antibacterial properties against <em>M. tuberculosis</em>.</p>
Data from: Antibacterial activity of graphene oxide nanosheet against multi drug resistant superbugs isolated from infected patients
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Phenotypic and genotypic analysis of drug resistance in M. tuberculosis isolates in Gansu, China
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Polyphasic characterization of carbapenem-resistant Klebsiella pneumoniae clinical isolates suggests vertical transmission of the blaKPC-3 gene
<p>Optical DNA mapping (ODM) data related to the study, presented in figures 2-6 in the paper.</p>
Data from: Assessment of markers of antimalarial drug resistance in Plasmodium falciparum isolates from pregnant women in Lagos, Nigeria
Background: The use of antimalarial drugs for prevention and treatment is a major strategy in the prevention of malaria in pregnancy. Although sulphadoxine-pyrimethamine (SP) is currently recommended for intermittent preventive treatment of malaria during pregnancy in Nigeria, previously used drugs for prophylaxis such as chloroquine (CQ) and pyrimethamine are accessible as they are purchased over the counter. This study describes the markers of absence or presence of resistance to quinoline (Pfcrt and Pfmdr 1) and type 1 antifolate antimalarial medicines (Pfdhfr). Methods: Plasmodium falciparum-positive dried blood spots from pregnant women attending antenatal clinics for the first time during current pregnancy were investigated for the presence of mutations at codons 72–76 of Plasmodium falciparum chloroquine resistance transporter (Pfcrt) gene by real time polymerase chain reaction (PCR) using haplotype-specific probes. PCR followed by sequence analysis was used to identify mutations at codons 86, 184, 1034, 1042 and 1246 of P. falciparum multi-drug resistance-1 (Pfmdr1) gene; and codons 16, 50, 51, 59, 108, 140 and 164 of Pfdhfr gene. Results: Two haplotypes of Pfcrt (n = 54) were observed: CVMNK 13(24.2%) and CVIET 41 (75.9%) of the samples. The SVMNT haplotype was absent in this population. The Pfmdr1 (n = 28) haplotypes were NYSND 15(53.6%), YYSND 5(17.9%), NFSND 6(21.4%) and YFSND 2(7.1%). The Pfdhfr (n = 15) were ACNCSVI 4(26.7%), and ACICNSVI 1(6.7%) and ACIRNVI 10 (66.7%). The rate of occurrence of Pfcrt 76T, Pfdhfr108N, Pfmdr186Yand184F were 75.9%, 73.3%, 25% and 28.1% respectively. The Pfmdr1 86Y was associated with low parasitaemia (median = 71 parasites/μl, P = 0.024) while Pfcrt 76T was associated with young maternal age (mean 24.1 ± 4.5 years; P = 0.006). The median parasitaemia were similar (P>0.05) in wild and mutant strains of Pfcrt 76, Pfmdr1 184 and Pfdhfr 108. There was no association between gravidity or gestational age of the women and presence of mutations in the Pfcrt, Pfmdr1 or Pfdhfr genes (P>0.05). Conclusion: Markers of resistance to chloroquine and pyrimethamine were high, whereas cycloguanil-resistance marker was not present in the studied population. The low level of mutations in the Pfmdr1gene indicates likely efficacy of amodiaquine against malaria in pregnancy.
16s rRNA and copLAB partial sequences of copper resistant Xanthomonas spp. isolated from agrochemical impacted crucifer fields in Trinidad
<p>Sanger sequence generated partial sequences of copLAB genes present in copper resistant Xanthomonas spp associated with leaf lesion and 16s rRNA sequences used for bacterial genera identification. Part of an upcoming manuscript, will be linked within document.</p>
States of genome assembly supporting data for complete genome assembly of clinical multidrug resistant Bacteroides fragilis isolates enables comprehensive identification of antimicrobial resistance genes and plasmids.
<p>Assemblies for each isolate and assembly stage is in .gfa and .fasta format.</p> <p>the best SPAdes assembly is also included in the .zip files.</p> <p>1) Unicycler with illumina data and Nanopore data from the first sequencing run, filtered with FiltLong.<br> 2) Unicycler with illumina data and Nanopore data from the first sequencing run, filtered with FiltLong and error corrected with Canu<br> 3) Unicycler with illumina data and Nanopore data from the first and second sequencing run, filtered with FiltLong.<br> 4) manual finshing of assembly 3. <br> Methods are described in the paper and at the github repository (https://github.com/thsyd/bfassembly)</p> <p> </p>
An evaluation of isolation by distance (IBD) and isolation by resistance (IBR) on genetic structure of the Persian squirrel (Sciurus anomalus) in the Zagros forests of Iran
<p>For conservation of wild species, it is important to understand how landscape change and land management can affect gene flow and movement. Landscape genetic analyses provide a powerful approach to infer effects of various landscape factors on gene flow, thereby informing conservation actions. The Persian squirrel is a keystone species in the woodlands and oak forests of Western Asia, where it has experienced recent habitat loss and fragmentation. We conducted landscape genetic analyses of individuals sampled in the northern Zagros Mountains of Iran (provinces of Kurdistan, Kermanshah, and Ilam), focusing on evaluation of isolation by distance (IBD) and isolation by resistance (IBR), using 16 microsatellite markers. The roles of geographical distance and landscape features including roads, rivers, developed areas, farming and agriculture, forests, lakes, plantation forests, rangelands, shrublands and rocky areas of varying canopy cover, and swamp margins on genetic structure were quantified using individual-based approaches and resistance surface modelling. We found a significant pattern of IBD but only weak support for an effect of forest cover on genetic structure and gene flow. It seems that geographical distance is an important factor limiting the dispersal of the Persian squirrel in this region. The results of the current study inform ongoing conservation programs for the Persian squirrel in the Zagros oak forest.</p>
Detection of Erythromycin Resistant Genes in Group B Streptococcus strains isolated in Georgia
<p>figure 1 - <strong>Antibiotic Susceptibility Test Results </strong><a href="#_msocom_1">[ac1]</a> </p> <p> <a href="#_msoanchor_1">[ac1]</a>Results of Antibiotic Susceptibility Tests</p> <p><strong>figure 2 - </strong></p> <p><strong>Positive PCR results</strong><a href="#_msocom_1">[ac1]</a> </p> <p> <a href="#_msoanchor_1">[ac1]</a>Positive results for presence of the genes in resistant strains</p> <p> </p>
Assessment of four in vitro phenotypic biofilm detection methods in relation to antimicrobial resistance in aerobic clinical bacterial isolates
<p><strong>Introduction</strong>: The lack of standardized methods for detecting biofilms continues to pose a challenge to microbiological diagnostics since biofilm-mediated infections induce persistent and recurrent infections in humans that often defy treatment with common antibiotics. This study aimed to evaluate diagnostic parameters of four in vitro phenotypic biofilm detection assays in relation to antimicrobial resistance in aerobic clinical bacterial isolates.</p> <p><strong>Methods</strong>: In this cross-sectional study, bacterial strains from clinical samples were isolated and identified following the standard microbiological guidelines. The antibiotic resistance profile was assessed through the Kirby-Bauer disc diffusion method. Biofilm formation was detected by gold standard tissue culture plate method (TCPM), tube method (TM), Congo red agar (CRA), and modified Congo red agar (MCRA). Statistical analyses were performed using SPSS version 17.0, with a significant association considered at p<0.05.</p> <p><strong>Result</strong>: Among the total isolates (n=226), TCPM detected 140 (61.95%) biofilm producers, with CoNS (9/9) (p<0.001) as the predominant biofilm former. When compared to TCPM, TM (n=119) (p<0.001) showed 90.8% sensitivity and 70.1% specificity, CRA (n=88) (p=0.123) showed 68.2% sensitivity and 42% specificity, and MCRA (n=86) (p=0.442) showed 65.1% sensitivity and 40% specificity. Juxtaposed to CRA, colonies formed on MCRA developed more intense black pigmentation from 24 to 96 hours. There were 77 multi-drug-resistant (MDR)-biofilm formers and 39 extensively drug-resistant (XDR)-biofilm formers, with 100% resistance to ampicillin and ceftazidime, respectively.</p> <p><strong>Conclusion</strong>: It is suggested that TM be used for biofilm detection. Unlike MCRA, black pigmentation in colonies formed on CRA declined with time. MDR- and XDR-biofilm formers were frequent among the clinical isolates.</p>
Microbiological Characterization and Antibiotic Resistance Profiling of Providencia Spp,: An Emerging Pathogen Isolated From Urinary Tract Infections
ClinicalTrials.gov study NCT07204769. IPD Sharing: NO. Countries: 1. Publications: 6.
Study of the Antibiotic-resistance Profile of Enterobacterales Isolated From Rectal Mucosal Buffer of PrEP Subjects
ClinicalTrials.gov study NCT07370948. IPD Sharing: NO. Countries: 1. Publications: 15.
Antimicrobial Susceptibility Patterns of N. Gonorrhea Isolates in an Era of Quinolone Resistance
ClinicalTrials.gov study NCT00692822. IPD Sharing: Not stated. Countries: 1. Publications: 5.
Detection of Efflux Pump Genes Mediating Ciprofloxacin Resistance in Staphylococcus Aureus Isolates in Sohag University Hospitals
ClinicalTrials.gov study NCT05828550. IPD Sharing: UNDECIDED. Countries: 1. Publications: 4.
Effects of Isolated Lumbar Extension Resistance Training Alone and in Combination with General Exercise and Manual Therapy
ClinicalTrials.gov study NCT06890052. IPD Sharing: NO. Countries: 1. Publications: 1.
Effects of Integrated And Isolated Resisted Exercise on Strength, Power And Endurance
ClinicalTrials.gov study NCT06131814. IPD Sharing: NO. Countries: 1. Publications: 6.
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