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FIGURE. Typical habitats of Ramalina species on northern South America. A. High paramo, Laguna Anteojos, Sierra Nevada de Merida, where grows on rocks R. anteojina at 4100 m. B. Sub-paramo (timberline), La Aguada, Sierra Nevada de Merida, 3100 m, where are found R. dictyota and R. reducta on shrubs. C. Andean cloud forest, La Victoria, Sierra Nevada de Merida where R. cochlearis, R. cumanensis and R. victoriana are found growing as epiphytes. D. Populations of R. usnea, R. morrocoyensis and R. paradisensis growing as epiphytes on mangroves and Suriana maritima at sea level, National Park Morrocoy, state Falcón; the latter two species are known only from this locality. E. Ramalina usnea is the only species of this genus reported from the Alto Orinoco, Amazonas, near La Esmeralda, 150 m, growing as corticolous in submontane forests, at the top of the picture the Cerro Duida. F. Xerophytic forests from the National Park Cerro Santa Ana, state Falcón, where Ramalina santanensis and R. microphylla are known only growing on soil and rocks at 200–400 m. Photos V. Marcano. in The genus Ramalina Acharius (Ascomycota, Lecanoromycetes, Ramalinaceae) in northern South America

FIGURE. Typical habitats of Ramalina species on northern South America. A. High paramo, Laguna Anteojos, Sierra Nevada de Merida, where grows on rocks R. anteojina at 4100 m. B. Sub-paramo (timberline), La Aguada, Sierra Nevada de Merida, 3100 m, where are found R. dictyota and R. reducta on shrubs. C. Andean cloud forest, La Victoria, Sierra Nevada de Merida where R. cochlearis, R. cumanensis and R. victoriana are found growing as epiphytes. D. Populations of R. usnea, R. morrocoyensis and R. paradisensis growing as epiphytes on mangroves and Suriana maritima at sea level, National Park Morrocoy, state Falcón; the latter two species are known only from this locality. E. Ramalina usnea is the only species of this genus reported from the Alto Orinoco, Amazonas, near La Esmeralda, 150 m, growing as corticolous in submontane forests, at the top of the picture the Cerro Duida. F. Xerophytic forests from the National Park Cerro Santa Ana, state Falcón, where Ramalina santanensis and R. microphylla are known only growing on soil and rocks at 200–400 m. Photos V. Marcano.

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FIGURE 4 in The genus Ramalina Acharius (Ascomycota, Lecanoromycetes, Ramalinaceae) in northern South America

FIGURE 4. View of the upper surface of R. canaguensis var. mucumpisensis showing round laminal soralia. Scale = 120 µm.

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FIGURE. View of the upper side in R. complanata showing a slightly sinuose and more or less rugose surface. Scale = 10.35 µm. in The genus Ramalina Acharius (Ascomycota, Lecanoromycetes, Ramalinaceae) in northern South America

FIGURE. View of the upper side in R. complanata showing a slightly sinuose and more or less rugose surface. Scale = 10.35 µm.

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FIGURES and. Apothecial surfaces. 27. Scabrose surface in R. venezuelensis, scale = 6.5 µm. 28. Microfilamentose surface in R. canaguensis var. mucumpisensis, scale = 8.75 µm. in The genus Ramalina Acharius (Ascomycota, Lecanoromycetes, Ramalinaceae) in northern South America

FIGURES and. Apothecial surfaces. 27. Scabrose surface in R. venezuelensis, scale = 6.5 µm. 28. Microfilamentose surface in R. canaguensis var. mucumpisensis, scale = 8.75 µm.

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FIGURE. View of medullary hyphae in R. subcalcarata covered by a thin layer of secreted material, probably glycoproteins. The presence of small, elongated and electron-dense nodules is also observed. Scale = 12 µm. in The genus Ramalina Acharius (Ascomycota, Lecanoromycetes, Ramalinaceae) in northern South America

FIGURE. View of medullary hyphae in R. subcalcarata covered by a thin layer of secreted material, probably glycoproteins. The presence of small, elongated and electron-dense nodules is also observed. Scale = 12 µm.

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Supplementary material 1 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Three Major Ecogeographic Areas of Evolution in Fruticose Ramalinaceae

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Supplementary material 6 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Table S6. Data for the Niebla collections studied

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Figure 6 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 6 Time-calibrated phylogenetic tree generated by the BEAST2 analysis on four loci (Matrix 1), for the four genera of fruticose Ramalinaceae studied and their crustose sister genus Cliostomum: Namibialina, Niebla, Ramalina and Vermilacinia. Values above branches represent the posterior probabilities of support.

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Figure 13 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 13 New species of saxicolous VermilaciniaA, BV. breviloba (holotype) C, DV. lacunosa (holotype) E, FV. pustulata (holotype) G, HV. reticulata (holotype). Scale: 1 mm (A, C). Photographs by R. Spjut.

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Figure 11 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 11 Species in RamalinaA, BR. krogiae (holotype) C–ER. requienii (France, Corsica). Scale: 1 cm (A–D); scale in E identical to D. Photographs by E. Sérusiaux.

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Figure 10 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 10 Ramalina breviuscula aggr A, BR. breviuscula, type locality in France, eastern Pyrenees (photographs taken in the field) C, DRamalina sp. 2 (France, Corsica). Scale: 1 mm (C, D). Photographs by E. Sérusiaux.

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Figure 8 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 8 Evolutionary tree for the genera Niebla and Vermilacinia, produced with the 6-locus matrix and using *BEAST (Matrix 2). Values above branches represent posterior probabilities of support. Epithets in colour following insert: green = collected in USA/California; pink = collected in Mexico/Baja California; blue = collected in Mexico/Baja California Sur. Similarity matrix from the STACEY analysis on the right. Each rectangle represents posterior probability (white = 0, black = 1) of pairs of specimens to belong to the same species. Shades of grey represent intermediate values. Rectangles delimited by red lines represent the species delimitation with a 0.3 cut-off.

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Supplementary material 8 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Identification of the chemistry of Ramalina rosacea

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Figure 12 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 12 Species in RamalinaAR. crispans (holotype: left-hand specimen; other specimen from Morocco: right-hand) BR. lusitanica (Morocco, leg. J. Gattefosse, BC) C, DR. lusitanica (Italy/Sardinia, accession LG DNA 1525). Scale: 1 mm (C). Photographs by E. Sérusiaux.

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Figure 3 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 3 A Landscape in Baja California, San Quintín where Ramalina crinita thrives BR. crinita; C Landscape of the Skeleton Coast Park in Namibia where Namibialina "angulosa" and N. melanothrix thrive DN. melanothrixE–FN. "angulosa 1" (E) and N. "angulosa 2" (F). Photographs by E. Sérusiaux.

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Supplementary material 7 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Table S7. Data for the Niebla collections studied

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Figure 1 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 1 Landscapes of Baja California and Baja California Sur. A Arenicolous species of Niebla in sand dunes at San Quintín BCB saxicolous species of Niebla over dark volcanic rocks at San Quintín BCC twigs of Lycium covered with fruticose Roccella and Vermilacinia at San Quintín BCD Free-living species of Niebla on "red" rocky slopes at Punta Baja BCE landscapes of the peninsula de Vizcaíno F gypsicolous rocks near the sea at Bahia Ascunsion BCS with Niebla lobulataG chaparral near Bahia Tortugas BCS H outcrops subjected to intermittent fog near Bahia Tortugas BCS. Photographs by E. Sérusiaux and R. Spjut.

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Supplementary material 2 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Table S2. PCR conditions and primers for each locus

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Figure 5 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 5 Several species of Niebla and Vermilacinia. Identifications based on Spjut (1996)ANiebla limicola on sand dunes at Guerrero Negro BCS BN. marinii at Santo Domingo BCCN. podetiaforma at Punta Baja BCDN. siphonoloba at San Antonio BCEVermilacinia cerebra at San Antonio BCFNiebla lobulata at Bahía de Tortugas BCS GVermilacinia leopardina on branches at San Quintín BCHV. procera on rocks at San Quintín BC. Photographs by E. Sérusiaux and R. Spjut.

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Supplementary material 3 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Table S3. Table of accessions of the lichen collections studied

opencc-zeroSep 2020View details →

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