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52 results for “Lettuce”
Microbiological and nutritional analysis of lettuce crops grown on the International Space Station-VEG03A
The ability to grow safe, fresh food to supplement packaged foods of astronauts in space has been an important goal for NASA. Food crops grown in space experience different environmental conditions than plants grown on Earth (e.g., reduced gravity, elevated radiation levels). To study the effects of space conditions, red romaine lettuce, Lactuca sativa cv ‘Outredgeous,’ plants were grown in Veggie plant growth chambers on the International Space Station (ISS) and compared with ground-grown plants. Multiple plantings were grown on ISS and harvested using either a single, final harvest, or sequential harvests in which several mature leaves were removed from the plants at weekly intervals. Ground controls were grown simultaneously with a 24–72 h delay using ISS environmental data. Food safety of the plants was determined by heterotrophic plate counts for bacteria and fungi, as well as isolate identification using samples taken from the leaves and roots. Molecular characterization was conducted using Next Generation Sequencing (NGS) to provide taxonomic composition and phylogenetic structure of the community. Leaves were also analyzed for elemental composition, as well as levels of phenolics, anthocyanins, and Oxygen Radical Absorbance Capacity (ORAC). Comparison of flight and ground tissues showed some differences in total counts for bacteria and yeast/molds (2.14 – 4.86 log10 CFU/g), while screening for select human pathogens yielded negative results. Bacterial and fungal isolate identification and community characterization indicated variation in the diversity of genera between leaf and root tissue with diversity being higher in root tissue, and included differences in the dominant genera. The only difference between ground and flight experiments was seen in the third experiment, VEG-03A, with significant differences in the genera from leaf tissue. Flight and ground tissue showed differences in Fe, K, Na, P, S, and Zn content and total phenolic levels, but no differences in anthocyanin and ORAC levels. This study indicated that leafy vegetable crops can produce safe, edible, fresh food to supplement to the astronauts’ diet, and provide baseline data for continual operation of the Veggie plant growth units on ISS.
Microbiological and nutritional analysis of lettuce crops grown on the International Space Station-VEG01B
Lettuce crops grown in the Veggie payload aboard ISS, harvested and returned for chemical, microbiological and molecular analysis
Effect of varying amounts of simulated lunar soil batch #005 upon growth of lettuce explants
The following general conclusions can be made concerning the use of tissue culture for assaying the effects of mineral elements from simulated lunar soil or other mineral samples: The method used here is a very sensitive one for showing that soluble materials are reaching the tissue explant. The effects found (inhibiting) are very probably caused by excess toxic materials or competition of non-essential elements with essential elements rather than pH changes because previously pH has been found- to have relatively little effect over a wide range. Any stimulatory effect of the simulated lunar soil will probably appear at concentrations below those used in these experiments. If this is the case, the method is much less wasteful of mineral materials than methods in which pre-grown callus is treated with mineral materials. Measurements of uptake of minerals will be difficult in this system because of the relatively small growth obtainable from the tissue and also because of the apparently large background of minerals contributed by the purified agar.
Apollo 17 Challenge System - Seed Germination (Cabbage, Brussel Sprouts, Radish and Lettuce)
Analyses of effects of lunar materials on plants: This study was designed to determine if lunar material can cause disease in plants grown under controlled conditions and to assess the effects of lunar materials on the germination and subsequent development of seeds of species belonging to the Tracheophyta (vascular plants). Due to the historic nature of these experiments some metadata is absent or unclear in the available documentation. At OSDR we welcome contributions from our community. If you review the material associated with this dataset and have a suggestion for an improvement, correction or addition please let us know at arc-dl-osdr-data@mail.nasa.gov.
Substrate Matters: Ionic Silver Alters Lettuce Growth, Nutrient Uptake, and Root Microbiome in a Hydroponics System
Ionic silver (Ag+) is being investigated as a residual biocide for use in NASA spacecraft potable water systems on future crewed missions. This water will be used to irrigate future spaceflight crop production systems. We have evaluated the impact of three concentrations (31 ppb, 125 ppb, and 500 ppb) of ionic silver biocide solutions on lettuce in an arcillite (calcinated clay particle substrate) and hydroponic (substrate-less) growth setup after 28 days. Lettuce plant growth was reduced in the hydroponic samples treated with 31 ppb silver and severely stunted for samples treated at 125 ppb and 500 ppb silver. No growth defects were observed in arcillite-grown lettuce. Silver was detectable in the hydroponic-grown lettuce leaves at each concentration but was not detected in the arcillite-grown lettuce leaves. Specifically, when 125 ppb silver water was applied to a hydroponics tray, Ag+ was detected at an average amount of 7 ug/g (dry weight) in lettuce leaves. The increase in Ag+ corresponded with a decrease in several essential elements in the lettuce tissue (Ca, K, P, S). In the arcillite growth setup, silver did not impact the plant root zone microbiome in terms of alpha diversity and relative abundance between treatments and control. However, with increasing silver concentration, the alpha diversity increased in lettuce root samples and in the water from the hydroponics tray samples. The genera in the hydroponic root and water samples were similar across the silver concentrations but displayed different relative abundances. This suggests that ionic silver was acting as a selective pressure for the microbes that colonize the hydroponic water. The surviving microbes likely utilized exudates from the stunted plant roots as a carbon source. Analysis of the root-associated microbiomes in response to silver showed enrichment of metagenomic pathways associated with alternate carbon source utilization, fatty-acid synthesis, and the ppGpp (guanosine 3'-diphosphate 5'-diphosphate) stringent response global regulatory system that operates under conditions of environmental stress. Nutrient solutions containing Ag+ in concentrations greater than 31 ppb in hydroponic systems lacking cation-exchange capacity can severely impact crop production due to stunting of plant growth.
Microbiological and nutritional analysis of lettuce crops grown on the International Space Station-VEG01A
The ability to grow safe, fresh food to supplement packaged foods of astronauts in space has been an important goal for NASA. Food crops grown in space experience different environmental conditions than plants grown on Earth (e.g., reduced gravity, elevated radiation levels). To study the effects of space conditions, red romaine lettuce, Lactuca sativa cv ‘Outredgeous,’ plants were grown in Veggie plant growth chambers on the International Space Station (ISS) and compared with ground-grown plants. Multiple plantings were grown on ISS and harvested using either a single, final harvest, or sequential harvests in which several mature leaves were removed from the plants at weekly intervals. Ground controls were grown simultaneously with a 24–72 h delay using ISS environmental data. Food safety of the plants was determined by heterotrophic plate counts for bacteria and fungi, as well as isolate identification using samples taken from the leaves and roots. Molecular characterization was conducted using Next Generation Sequencing (NGS) to provide taxonomic composition and phylogenetic structure of the community. Leaves were also analyzed for elemental composition, as well as levels of phenolics, anthocyanins, and Oxygen Radical Absorbance Capacity (ORAC). Comparison of flight and ground tissues showed some differences in total counts for bacteria and yeast/molds (2.14 – 4.86 log10 CFU/g), while screening for select human pathogens yielded negative results. Bacterial and fungal isolate identification and community characterization indicated variation in the diversity of genera between leaf and root tissue with diversity being higher in root tissue, and included differences in the dominant genera. The only difference between ground and flight experiments was seen in the third experiment, VEG-03A, with significant differences in the genera from leaf tissue. Flight and ground tissue showed differences in Fe, K, Na, P, S, and Zn content and total phenolic levels, but no differences in anthocyanin and ORAC levels. This study indicated that leafy vegetable crops can produce safe, edible, fresh food to supplement to the astronauts’ diet, and provide baseline data for continual operation of the Veggie plant growth units on ISS.
Escherichia coli Gene Expression Profiling in Response to the Interaction with Lettuce Rhizosphere
GEO Series GSE33735. Escherichia coli; Escherichia coli str. K-12 substr. MG1655. 6 samples. Type: Expression profiling by array.
Global changes in gene expression in Caco-2 cell line by treatment with extract from iodine-biofortified carrot and lettuce
GEO Series GSE71605. Homo sapiens. 15 samples. Type: Expression profiling by array.
New insight on enzymatic browning mechanism by comparative transcriptome analysis of romaine lettuce cultivars with different browning potentials
GEO Series GSE185049. Lactuca sativa. 18 samples. Type: Expression profiling by high throughput sequencing.
Transcription profiling of lettuce in response to UV-B radiation
GEO Series GSE105821. Lactuca sativa. 8 samples. Type: Expression profiling by high throughput sequencing.
Comparison Gene Expression and Biochemical Profile of Escherichia coli K12 and O157:H7 Interaction with Lettuce Rhizosphere
GEO Series GSE36885. Escherichia coli. 6 samples. Type: Expression profiling by array.
LsCLE12 peptide delays senescence in lettuce and prolongs postharvest life of roses
GEO Series GSE299676. Lactuca sativa. 20 samples. Type: Expression profiling by high throughput sequencing.
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