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59 results for “Metagenomic analysis”
Metagenomic analysis of Mesolithic chewed pitch reveals poor oral health among stone age individuals
<p>Prehistoric chewed pitch has proven to be a useful source of ancient DNA, both from humans and their microbiomes. Here we present the metagenomic analysis of three pieces of chewed pitch from Huseby Klev, Sweden, that were dated to 9,890–9,540 before present. The metagenomic profle exposes a Mesolithic oral microbiome that includes opportunistic oral pathogens. We compared the data with healthy and dysbiotic microbiome datasets and we identifed increased abundance of periodontitisassociated microbes. In addition, trained machine learning models predicted dysbiosis with 70–80% probability. Moreover, we identifed DNA sequences from eukaryotic species such as red fox, hazelnut, red deer and apple. Our results indicate a case of poor oral health during the Scandinavian Mesolithic, and show that pitch pieces have the potential to provide information on material use, diet and oral health.</p>
Exploring the Atmospheric Microbiome in the Eastern Mediterranean through Metagenomic Analysis
<p><span>Dataset</span><span> S5 (separate file)</span><span>. </span><span>Genome coverage depth information for each specific position across the entire genome of potential pathogens.</span></p>
Metagenomic Analysis of the Link Between Periodontitis and Smoking: Case-control Study
ClinicalTrials.gov study NCT05995158. IPD Sharing: NO. Countries: 1. Publications: 0.
Metagenomic and Metatranscriptomic Analysis of Clinical Plaque Samples
ClinicalTrials.gov study NCT05138978. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Metagenomic Analysis of Human Gut Microbiota in Patients With Metabolic Diseases Including Diabetes
ClinicalTrials.gov study NCT03204799. IPD Sharing: NO. Countries: 1. Publications: 0.
Metagenomic Analysis of Gut Microbiota in Untreated and Uncontrolled Hypertensive Patients
ClinicalTrials.gov study NCT06284928. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Metagenome Analysis of Gut Microbiota in CC Patients
ClinicalTrials.gov study NCT02395484. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Metagenomic Analysis of Bacterial Microbiota in Breast Milk
ClinicalTrials.gov study NCT06037421. IPD Sharing: NO. Countries: 1. Publications: 0.
Metagenomic Analysis of the Oral Microbiome in Patients With Oral Lichen Planus: A Pilot Study in the Turkish Population
ClinicalTrials.gov study NCT07137156. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Metagenomic Analysis of Gut Microbiome in Korean Patients With Ulcerative Colitis
ClinicalTrials.gov study NCT01010815. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Clinical Effects and Metagenomic Analysis of Using an Air Polishing Device With Erythritol Powder Compared to Scaling and Root Planing in Periodontal Maintenance Therapy.
ClinicalTrials.gov study NCT07344805. IPD Sharing: NO. Countries: 1. Publications: 0.
Data from: Integrated metabolomics and metagenomics analysis of plasma and urine identified microbial metabolites associated with coronary heart disease
Open the record for dataset details and reuse information.
Metagenomic analysis of phytoplasma infected apple trees
GEO Series GSE306190. Malus domestica. 6 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Metagenomic and Immune-inflammatory Analysis of Individuals With Generalized Aggressive Periodontitis and Their Descendants.
ClinicalTrials.gov study NCT03933514. IPD Sharing: UNDECIDED. Countries: 0. Publications: 0.
Metagenomic analysis of feces from mice flown on the RR-6 mission
The objective of the Rodent Research-6 (RR-6) study was to evaluate muscle atrophy in mice during spaceflight and to test the efficacy of a novel therapeutic to mitigate muscle wasting. The experiment involved an implantable subcutaneous nanochannel delivery system (nDS; between scapula), which delivered the drug formoterol (FMT; a selective β2 adrenoceptor agonist), over the course of time. To this end, a cohort of forty 32-weeks-old female C57BL/6NTac mice were either sham operated or implanted with vehicle or treatment-filled nDS, launched in two Transporters (20 mice per Transporter) on SpaceX-13 on December 15, 2017. They were transferred to Rodent Habitats onboard the International Space Station (ISS), and maintained in microgravity for 29 days (N=20, Live Animal Return Spaceflight [LAR FLT]), or >50 days (N=20, ISS Terminal Spaceflight [ISS-T FLT]). After 29 days, the 20 LAR FLT animals were returned live to back to Earth on January 13, 2018. After splashdown, the animals were ambulatory on-ground for ~4 days, until all subjects were processed during one day of dissections. There were two Basal (BSL) groups of animals sacrificed (LAR BSL & ISS-T BSL; N=20; 40 animals; ~36 weeks old) at Kennedy Space Center (KSC; 12/9/17). LAR BSL animals were dissected, and samples were collected upon euthanasia. A Ground Control (GC) group, LAR GC, mimicked the LAR FLT group, which was housed at KSC, then shipped alive, to Novartis’s Facilities, where both the LAR FLT and LAR GC groups were processed (~41 weeks old; 1/16/18). All were anesthetized with isoflurane, blood samples were obtained by closed-chest cardiac puncture, and the animals were euthanized by exsanguination and thoracotomy. The 20 ISS-T FLT mice were anesthetized via intraperitoneal injection of ketamine/xylazine/acepromazine over the course of a four days of dissections (2/6/18 until 2/9/18; 53-56 days after launch; 44 weeks old at time of on-orbit dissections). Blood samples and euthanasia were conducted the same as LAR groups. Following blood draw and hind limb dissection, the ISS-T FLT animal carcasses were wrapped in aluminum foil, placed in a ziploc bag and placed in storage at -80˚C or colder until return. The ISS-T Ground Control (ISS-T GC) (at KSC) followed the same euthanasia timeline, methods, and preservation. The final processing of frozen ISS-T FLT, frozen ISS-T GC and frozen 0-day ISS-T BSL animals were completed at Houston Methodist Research Institute, in Houston, TX (5/21/18 until 5/24/18). GeneLab received feces from only sham treated animals (no drug treated animals) from the following groups. FLT: LAR (n=9), ISS-T (n=7); GC: LAR (N=7), ISS-T (N=9); BSL: LAR (n=7), ISS-T (n=9). DNA was extracted and analyzed by sequencing using a variety of different targeted and un-targeted metagenome profiling assays.
Metagenomic analysis of feces from mice flown on the RR-9 mission
The objective of the Rodent Research-9 (RR-9) mission was to use mice to understand the molecular basis of phenomena that affect astronauts during long-duration spaceflight, particularly visual impairment and joint tissue degradation. To this end, a flight group (FLT) of 10-week-old male C57BL/6J mice was launched from Kennedy Space Center (KSC) on 8/14/2017 and housed in Rodent Habitats on the ISS for 33 days before being returned alive to Earth. After splashdown in the Pacific Ocean, the animals were transported to Loma Linda University (LLU) for testing, euthanasia and dissection on 9/18/2018. A Basal Control (BSL) was housed in standard cages at Kennedy Space Center (KSC) and euthanized one day after launch of the FLT animals (8/15/2017). Ground Control (GC) and Vivarium Control (VIV) studies were planned to commence at KSC approximately one-week after the conclusion of the flight experiments. However, all the GC and VIV mouse studies at KSC had to be cancelled due to Hurricane Irma and potential adverse effects on the animal housing facility. The GC and VIV studies were therefore rescheduled and begun in May, 2018. The GC was euthanized and dissected 6/18/2018 - 6/20/2018, while the VIV was euthanized and dissected 6/22/2018 - 6/23/2018. Because this resulted in a different cohort of mice being used for the GC and VIV controls as compared to the flight (FLT) and basal (BSL) groups, two cohort controls were included in the study. The first, Cohort Control 1 (CC_C1), was from the same cohort as the FLT and BSL animals, and was sacrificed and dissected 4 days after the FLT group (9/22/2017). The second, Cohort Control 2 (CC_C2), was from the same cohort as the GC and VIV animals, and was sacrificed and dissected 2-8 days after the GC and VIV groups, (6/24/2018 - 6/26/2018). The CC_C1 and CC_C2 groups were housed in standard cages and fed standard chow in contrast to all other groups which received Rodent Foodbars. To clarify the connections between treatment groups and animal cohorts, the following group abbreviations are used in the sample metadata: Flight (FLT_C1); Basal (BSL_C1); Ground Control (GC_C2); Vivarium Control (VIV_C2), Cohort Control 1 (CC_C1); Cohort Control 2 (CC_C2). Fecal pellets were isolated directly from mice during dissection and preserved by flash freezing in liquid nitrogen before stored at -80 C. DNA was then extracted, shotgun metagenomic libraries generated, and libraries sequenced (target 10 M clusters at PE 250 bp). Metagenomic data was generated from the following groups: Basal Control (n=5), Ground Control (n=5), Vivarium Control (n=5), Cohort Control 1 (n=5), Cohort Control 2 (n=5), Flight (n=5).
Metagenomic analysis revealed higher microbial and functional gene diversities in deep landfill
GEO Series GSE68712. Bacteria. 15 samples. Type: Other.
Metagenomic analysis revealed the microbial-mediated soil organic carbon loss under the degeneration succession in alpine meadow
GEO Series GSE93158. uncultured soil microorganism. 20 samples. Type: Other.
Taxonomic and functional diversity of the microbiome in a jet fuel contaminated site as revealed by combined application of in situ microcosms with metagenomic analysis
<p>Natural attenuation represents all processes that govern contaminant mass removal, which mainly occurs via microbial degradation in the environment. Although this process is intrinsic its rate and efficiency depend on multiple factors. This study aimed to characterize the microbial taxonomic and functional diversity in different aquifer sediments collected in the saturated zone and <em>in situ</em> microcosms (BACTRAP®s) amended with hydrocarbons (<sup>13</sup>C-labeled and non-labeled benzene, toluene and naphthalene) using 16S rRNA gene and “<em>shotgun</em>” Illumina high throughput sequencing at a jet-fuel contaminated site. The BACTRAP®s were installed to assess hydrocarbon metabolism by native bacteria. Results indicated that Proteobacteria, Actinobacteria and Firmicutes were the most dominant phyla (~98%) in the aquifer sediment samples. Meanwhile, in the benzene- and toluene-amended BACTRAP®s the phyla Firmicutes and Proteobacteria accounted for about 90% of total community. In the naphthalene-amended BACTRAP®, members of the SR-FBR-L83 family (Order Ignavibacteriales) accounted for almost 80% of bacterial community. Functional annotation of metagenomes showed that only the sediment sample located at the source zone border and with the lowest BTEX concentration, has metabolic potential to degrade hydrocarbons aerobically. On the other hand, <em>in situ</em> BACTRAP®s allowed enrichment of hydrocarbon-degrading bacteria. Metagenomic data suggest that fumarate addition is the main mechanism for hydrocarbon activation of toluene. Also, indications for methylation, hydroxylation and carboxylation as activation mechanisms for benzene anaerobic conversion were found. After 120 days of exposure in the contaminated groundwater, the isotopic analysis of fatty acids extracted from BACTRAP®s demonstrated the assimilation of isotopic labeled compounds in the cells of microbes expressed by strong isotopic enrichment. We propose that the microbiota in this jet-fuel contaminated site has metabolic potential to degrade benzene and toluene by a syntrophic process, between members of the families Geobacteraceae and Peptococcaceae (genus <em>Pelotomaculum</em>), coupled to nitrate, iron and/or sulfate reduction.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.