Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
293
datasets available to search
ShareScore release 0.7.1
Dataset results
293 results for “Phase Separation”
Phase separation of SARS-CoV-2 nucleocapsid protein with TDP-43 is dependant on C-terminus domains
Open the record for dataset details and reuse information.
Experimental and Simulation data for the molecular mechanism of temperature-dependent phase separation of HSF1
<p>1. lmp_datatfile</p> <p>The documents contain the LAMMPS input datafiles of initial configuration used to run all simulations, including single chain, trimer(LZ1-3-RD), and RD systems.</p> <p>2.Experimental data.</p> <p>The documents contain original ucsf data</p>
sunflower oil - water emulsion estabilized by BSFLP protein: phase separation simulation results 0.48 h
<p>Animation of the simulation results presented in Figure 7 of Journal of Food Engineering, Volume 387 p. 112331, available at <a title="Persistent link using digital object identifier" href="https://doi.org/10.1016/j.jfoodeng.2024.112331" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/j.jfoodeng.2024.112331</a></p> <p>The separation time in the video is dimensionless, and it corresponds to a 0 - 48h period</p>
Mechanistic dissection of increased enzymatic rate in a phase-separated compartment
<p><span>Biomolecular condensates concentrate macromolecules into discrete cellular foci without an encapsulating membrane. Condensates are often presumed to increase enzymatic reaction rates through increased concentrations of enzymes and substrates (mass action), although this idea has not been widely tested and other mechanisms of modulation are possible. Here we describe a synthetic system where the SUMOylation enzyme cascade is recruited into engineered condensates generated by liquid-liquid phase separation of multidomain scaffolding proteins. SUMOylation rates can be increased up to 36-fold in these droplets compared to the surrounding bulk, depending on substrate K<sub>M</sub>. This dependency produces substantial specificity among different substrates. Analyses of reactions above and below the phase separation threshold lead to a quantitative model in which reactions in condensates are accelerated by mass action and by changes in substrate K<sub>M</sub>, likely due to scaffold-induced molecular organization. Thus, condensates can modulate reaction rates both by concentrating molecules and by physically organizing them.</span></p>
Aberrant phase separation and nucleolar dysfunction can underlie rare genetic diseases
<p>This dataset provides companion data to the paper "Aberrant phase separation and nucleolar dysfunction can underlie rare genetic diseases". It includes raw data, images and computational code to generate the figures in the study.</p>
Repository for 'Surface-induced phase separation of reconstituted nascent integrin clusters on lipid membranes'
<p>Surface-induced phase separation of reconstituted nascent integrin clusters on lipid membranes (PNAS)</p> <p>The structure of this repository is as follows:</p> <p>1. FRAP_curves.zip -- contains FRAP curves </p> <ul> <li>FRAP_curves_for_fig2F_figS5</li> <li>FRAP_curves_for_fig2G</li> <li>FRAP_curves_for_fig2H</li> <li>FRAP_curves_for_fig2I</li> <li>FRAP_curves_for_fig2J</li> <li>FRAP_curves_for_fig2K</li> <li>FRAP_curves_for_figS8&E</li> </ul> <p>2. Images.zip -- contains confocal images epifluorescence images</p> <ul> <li>Images_for_fig1G_fig1H</li> <li>Images_for_fig3D</li> <li>Images_for_figS1</li> <li>Images_for_figS2C</li> <li>Images_for_figS3C</li> <li>Images_for_figS6</li> </ul> <p>3. Source_data.xlsx -- contains the data for plotting the Figures</p>
A phase-separated nuclear GBPL circuit controls immunity in plants
Open the record for dataset details and reuse information.
Mechanistic dissection of increased enzymatic rate in a phase-separated compartment
Open the record for dataset details and reuse information.
Binary phase separation 3D
<p>Time evolution of a binary phase separating polymer system using Cahn Hilliard equation in three dimensions.</p>
Dataset: On the Aggregation and Nucleation Mechanism of the Monoclonal Antibody Anti-CD20 Near Liquid- Liquid Phase Separation (LLPS)
<p>Dataset related to the paper: Pantuso et al., On the Aggregation and Nucleation Mechanism of the Monoclonal Antibody Anti-CD20 Near Liquid-Liquid Phase Separation (LLPS), Scientific Reports (2020) 10:8902.</p>
Coarse-grained simulations of a phase-separated membrane with different LINCS settings (part 2/3)
<p>systems with lincs_iter = 2. To be written.</p>
Coarse-grained simulations of a phase-separated membrane with different LINCS settings (part 1/3)
<p>systems with lincs_iter = 1. To be written.</p>
Coarse-grained simulations of a phase-separated membrane with different LINCS settings (part 3/3)
<p>Additional simulations with lincs_order 10 & 12</p>
Molecular determinants of phase separation for Drosophila DNA replication licensing factors
<p>Liquid-liquid phase separation (LLPS) of intrinsically disordered regions (IDRs) in proteins can drive the formation of membraneless compartments in cells. Phase-separated structures enrich for specific partner proteins and exclude others. Previously, we showed that the IDRs of metazoan DNA replication initiators drive DNA-dependent phase separation <i>in vitro</i> and chromosome binding <i>in vivo</i>, and that initiator condensates selectively recruit partner proteins (Parker et al., 2019). How initiator IDRs facilitate LLPS and maintain compositional specificity is unknown. Here, using <i>D. melanogaster (Dm)</i> Cdt1 as a model initiation factor, we show that phase separation results from a synergy between electrostatic DNA-bridging interactions and hydrophobic inter-IDR contacts. Both sets of interactions depend on sequence composition (but not sequence order), are resistant to 1,6-hexanediol, and do not depend on aromaticity. These findings demonstrate that distinct sets of interactions drive condensate formation and specificity across different phase-separating systems and advance efforts to predict IDR LLPS propensity and partner selection <i>a priori</i>.</p>
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation
<p><span><span><span><span><span><span><span><span><span><span><span>Integrin adhesion complexes (IACs) are integrin-based plasma membrane-associated compartments where cells sense environmental cues. The physical mechanisms and molecular interactions that mediate initial IAC formation are unclear. We found that both p130Cas ("Cas") and Focal adhesion kinase ("FAK") undergo liquid-liquid phase separation <i>in vitro</i>under physiologic conditions. Cas- and FAK- driven phase separation is sufficient to reconstitute kindlin-dependent integrin clustering <i>in vitro </i>with recombinant mammalian proteins. <i>In vitro</i> condensates and IACs in mouse embryonic fibroblasts (MEFs) exhibit similar sensitivities to environmental perturbations including changes in temperature and pH. Furthermore, mutations that inhibit or enhance phase separation <i>in vitro</i> reduce or increase the number of IACs in MEFs, respectively. Finally, we find that the Cas and FAK pathways act synergistically to promote phase separation, integrin clustering, IAC formation and partitioning of key components <i>in vitro</i> and in cells. We propose that Cas- and FAK- driven phase separation provides an intracellular trigger for integrin clustering and nascent IAC formation.</span></span></span></span></span></span></span></span></span></span></span></p>
Uncovering intermolecular interactions driving liquid-liquid phase separation of TDP-43 low complexity domain
Open the record for dataset details and reuse information.
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation
Open the record for dataset details and reuse information.
Molecular determinants of phase separation for Drosophila DNA replication licensing factors
Open the record for dataset details and reuse information.
Data from: Modeling human population separation history using physically phased genomes
Open the record for dataset details and reuse information.
Histone H2B.8 compacts flowering plant sperm through chromatin phase separation
GEO Series GSE161366. Arabidopsis thaliana. 48 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Other.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.