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FIGURE 4 in Contribution to the morphological description of the marine leech, Ozobranchus margoi (Apáthy) (Rhynchobdellida: Ozobranchidae) by using combined histology, micro-CT and SEM
FIGURE 4. Histological transversal section of O. margoi. A, section of the two lateral post ceca (pca), showing the central posterior intestine (pi) and empty lateral diverticula (ld). Scale bar = 500 µm. B, section trough the male system. The atrium is folded in two parts. The circular posterior part (pp) tied to the celomic cavity trough a connection (cn) and the dorso-ventrally flattened anterior part (ap) ventrally located with a central elongated empty canal (c). On the top of the atrium there is the muscular bulb (mb). Scale bar = 100 µm. C, the two ovaries (ov) containing the ovary cords develops beneath the posterior crop (pc). One of the two ejaculatory ducts (ed) and the ventral located nerve cord (nc) are visible. Scale bar = 100 µm. D, section at the level of the posterior crop (pc) filled with prey's blood. Beneath the posterior crop is possible to observe the two ovaries (o). The ovary on the left shows the terminal part of the egg string with two maturing irregular shaped oocytes (asterisk). On both sides of the posterior crop is visible one pair of testes (t) connected with the body wall trough connections (cn). In the left testis is visible the vasa efferentia (ve) diverting dorsally. Scale bar = 500 µm.
FIGURE 3 in Contribution to the morphological description of the marine leech, Ozobranchus margoi (Apáthy) (Rhynchobdellida: Ozobranchidae) by using combined histology, micro-CT and SEM
FIGURE 3. Histological transversal section of O. margoi A, section of the anterior crop (ac) including the last part of the oesophagus (oe) and columns of gland cells (gc) ending in a gland duct (gd). Scale bar = 500 µm. B, section trough the female system exposing the vagina (v) with the set of duct of the seminal receptaculum (sr). Laterally two tiny ejaculatory ducts (ed) and the atrium (a) are shown. Scale bar = 500 µm. C, section of the dorsal part of the anterior sucker showing one of the two pigmented cup eyes with pigmented supportive cells (psc) and photoreceptor cells (phc). Scale bar = 50 µm. D, transversal section of the eye showing two photoreceptors. The apical part of those cells is composed by a vacuole-like structure (vls) located above a large cytoplasmic area containing the nucleus (n). Beside the nucleus nerve fibres (nf) are departing. Between the two eyes is visible the body cavity (cc). Scale bar = 50 µm. E, section of the ganglionic mass at the level of the supraesophageal ganglion (sg) surrounding the proboscis (pr). Around the ganglionic mass is visible the body cavity (cc). Scale bar = 200 µm. F, section along the oviducal bridge with the two tubular ovaries (ov) opening in two foramina (fac, fnc) for the passage of the alimentary canal (ac) and the nerve cord (nc). Down of the ovaries there are the two seminal vesicles (sv). Scale bar = 500 µm.
FIGURE 5 in Contribution to the morphological description of the marine leech, Ozobranchus margoi (Apáthy) (Rhynchobdellida: Ozobranchidae) by using combined histology, micro-CT and SEM
FIGURE 5. Histological transversal section of O. margoi. A, different stage of maturation of clusters of germ cells in the testes. Spermatogonia (sg) and spermatids (st) in each cluster are arranged around the cythophore (cy). In more advanced stages it is possible to observe the tails (t) and degenerating cytophores (asterisk). Scale bar = 100 µm. B, structure of the ovary. The ovisac (os) surround the ovary cord (oc) immersed in the ovary lumen (ol). Elongated follicular cells (fc) envelope the egg string that is composed by numerous immature germ cells (gc). The club-shaped end (cse) and a tiny convoluted central part of the cord are visible in this section. Scale bar = 100 µm. C, maturing oocytes (o) in proximity of nurse cells (nc). The germinal vesicle or nucleus (n) contains pale nucleoplasm (np) and nucleoli (nl). Scale bar = 50 µm.
FIGURE 2 in Contribution to the morphological description of the marine leech, Ozobranchus margoi (Apáthy) (Rhynchobdellida: Ozobranchidae) by using combined histology, micro-CT and SEM
FIGURE 2. Scanning electron micrographs of the sensilla. A, B, ventral view of the anterior sucker showing two rings of sensilla (arrows), the external one with smaller sensilla. C, structure of a mouth sensillum showing a central overhead region delimited by a surrounding rime (sr) with cilia (c). D, particular of the anterior sucker showing numerous pores (arrows) beneath the rings of sensilla. E, ventral surface of the body showing regular pattern of sensilla (arrows) disposed in rows and alternate with pores. F, structure of a body sensillum showing short grouped cilia (g) and long single cilia (s) in the proximity of a pore.
FIGURE 1 in Contribution to the morphological description of the marine leech, Ozobranchus margoi (Apáthy) (Rhynchobdellida: Ozobranchidae) by using combined histology, micro-CT and SEM
FIGURE 1. Ozobranchus margoi (Apáthy, 1890) scanning electron micrographs. A, ventral view showing the anterior (as) and posterior sucker (ps). B, dorsal view showing the gills (g) and the typical external annulation (an). C, morphology of the gills (g). Each pair of gills arising from a single biannulate somite of the urosome composed of (a1 + a2)> a3, with a basal stalk (bs) dividing in secondary branches (sb) whence single filaments (f) are departing. D, ventral lateral view of the anterior sucker showing the atrium (a) extruded through the genital aperture (ga). E, ventral view of the branchiate segment showing the approximate position of the genital aperture (ga). F, occasionally a longitudinal canal was observed running the external surface of the filament (lc).
Few juveniles or males were collected. Only four males from groups 7, 8, 9, and 11, all in clade D, were included in the dataset. The male in Fig. 13E–H conforms to the general morphological description of males in Lobocriconema with an undifferentiated labial region, the absence of a stylet, a degenerate pharyngeal region, a FIGURE 7. SEM images of specimens representing clades D (A–H) and B (I). NID numbers are associated with unique specimens, all are females except image C. A) Lobocriconema sp., face view with conspicuous labial disc surrounded by irregular labial structure, Nine-Mile Prairie, Nebraska, NID 4533. B) Lobocriconema sp., face view lacking submedian lobes and displaying subcuticular labial structure, Big Thicket National Preserve, Texas, NID 4560. C) Lobocriconema sp., juvenile, head with visible submedian lobes, body scales with fine terminal projections, Spring Creek Prairie, Nebraska, NID 4514. D) Lobocriconema sp., face view lacking submedian lobes and displaying subcuticular labial structure, Nine-Mile Prairie, Nebraska, NID 4527 E) Lobocriconema sp., cephalic profile with protruding stylet, Nine-Mile Prairie, Nebraska, NID 4529. F) Lobocriconema sp., head profile lacking submedian lobes, Tunica Hills, Louisiana, NID 4574. G) Lobocriconema sp., tail with closed vulva, Nine-Mile Prairie, Nebraska, NID 4533. H) Lobocriconema sp., tail with closed vulva, Nine-Mile Prairie, Nebraska, NID 4526. I) Lobocriconema sp., face view lacking submedian lobes, Great Smoky Mountains National Park, Purchase Knob, NID 4570. in Species discovery and diversity in Lobocriconema (Criconematidae: Nematoda) and related plant-parasitic nematodes from North American ecoregions
Few juveniles or males were collected. Only four males from groups 7, 8, 9, and 11, all in clade D, were included in the dataset. The male in Fig. 13E–H conforms to the general morphological description of males in Lobocriconema with an undifferentiated labial region, the absence of a stylet, a degenerate pharyngeal region, a FIGURE 7. SEM images of specimens representing clades D (A–H) and B (I). NID numbers are associated with unique specimens, all are females except image C. A) Lobocriconema sp., face view with conspicuous labial disc surrounded by irregular labial structure, Nine-Mile Prairie, Nebraska, NID 4533. B) Lobocriconema sp., face view lacking submedian lobes and displaying subcuticular labial structure, Big Thicket National Preserve, Texas, NID 4560. C) Lobocriconema sp., juvenile, head with visible submedian lobes, body scales with fine terminal projections, Spring Creek Prairie, Nebraska, NID 4514. D) Lobocriconema sp., face view lacking submedian lobes and displaying subcuticular labial structure, Nine-Mile Prairie, Nebraska, NID 4527 E) Lobocriconema sp., cephalic profile with protruding stylet, Nine-Mile Prairie, Nebraska, NID 4529. F) Lobocriconema sp., head profile lacking submedian lobes, Tunica Hills, Louisiana, NID 4574. G) Lobocriconema sp., tail with closed vulva, Nine-Mile Prairie, Nebraska, NID 4533. H) Lobocriconema sp., tail with closed vulva, Nine-Mile Prairie, Nebraska, NID 4526. I) Lobocriconema sp., face view lacking submedian lobes, Great Smoky Mountains National Park, Purchase Knob, NID 4570.
FIGURES 16–22. Staurosirella rhombus, SEM. 16, 17 in Staurosirella rhombus (Ehrenberg), Ognjanova-Rumenova, Buczkó, Wojtal & R. Jahn, comb. nov.-Typification, morphology and biostratigraphic significance
FIGURES 16–22. Staurosirella rhombus, SEM. 16, 17. External view of valves showing features of valve outline, sternum, striae and spines. 18, 19. Apical pore fields. 20. Striae structures. 21. Ribbon like colony. Note the number and the width of the girdle bands. 22. The diffusely branched spines. Scale bars = 1μm (Figs. 18, 19, 20, 22), 5 μm (Figs. 16, 17), 10 μm (Fig. 21).
FIGURES 45–51. Achnanthes trachyderma. Individuals from Society Archipelago, SEM. 45 in Achnanthes citronella, A. trachyderma comb. nov. (Bacillariophyta) and allied taxa pertaining to the same morphological group
FIGURES 45–51. Achnanthes trachyderma. Individuals from Society Archipelago, SEM. 45. SV external side, with narrow sternum. 46. Apices with areolae (arrowhead). 47. Valvocopulae system lacking fimbriae (arrowhead). 48. SV internal side, with open SVVC (arrow) and no fimbriae (arrowheads). 50. Internal SV areolae pattern and no areolae on apices. 49. Detail of the cingulum composed of the two valvocopulae (SVVC and RVVC) and a supplementary copula (C). 51. Detail of the internal structure of the SV areolae. Scale bars = 5 μm (Figs 45–48, 50); 1 μm (Figs 49, 51).
FIGURES 52–57. Achnanthes trachyderma. Individuals from Society Archipelago, SEM. 52 in Achnanthes citronella, A. trachyderma comb. nov. (Bacillariophyta) and allied taxa pertaining to the same morphological group
FIGURES 52–57. Achnanthes trachyderma. Individuals from Society Archipelago, SEM. 52. RV external side with laciniate stauros reaching almost the margin. 53. RV internal side with the stauros marginally delineated by very short striae (arrow), low helictoglossa (arrowhead). 54. RV external side with large stauros (arrowheads). 55. Detail of the central area in internal side, with areola domed hymenes (arrowhead). 56. RV apex with strongly bent striae with a marginal row of larger areolae. 57. Terminal raphe fissure continuing on the mantle. Scale bars = 5 μm (Figs 52–54); 1 μm (Figs 55–57).
FIGURES 27–32. Cocconeis sawensis from Brazil. 27, 28, 30 TEM. 29, 31, 32 SEM. 27. Raphe valve whit valvocopula attached. 28 in Additional morphological features of the epiphytic diatom Cocconeis sawensis Al-Handal & Riaux-Gobin (Cocconeidaceae, Bacillariophyta) from a coastal lagoon, Southern Brazil
FIGURES 27–32. Cocconeis sawensis from Brazil. 27, 28, 30 TEM. 29, 31, 32 SEM. 27. Raphe valve whit valvocopula attached. 28. Detail of fimbriae coinciding with each interstria. 29. Raphe valve with valvocopula attached. Note the valvocopula open. 30. Detail of long fimbriae of unequal length and shape. 31, 32. Internal view of the sternum valve with valvocopula of raphe valve attached. Scale bars = 0.5 μm (Fig. 28), 1 μm (Figs 29–32), 2 μm (Fig. 27).
FIGURES 13–20. Cocconeis sawensis from Brazil. Raphe valves. 13–18. SEM. 19, 20. TEM. 13, 15 in Additional morphological features of the epiphytic diatom Cocconeis sawensis Al-Handal & Riaux-Gobin (Cocconeidaceae, Bacillariophyta) from a coastal lagoon, Southern Brazil
FIGURES 13–20. Cocconeis sawensis from Brazil. Raphe valves. 13–18. SEM. 19, 20. TEM. 13, 15. External view of the valve. 14. Internal view of the valve showing an irregular and raised marginal rim. 16, 17. Detail of the internal valves ends. Note raphe lying in a very narrow raised axial area and distal raphe ending a small helictoglossae. 18. Detail of the central area round and slightly raised with raphe endings deflected in opposite directions. 19. Valve. 20. Detail of hymenes occluding areolae with radial perforations. Scale bars = 200 nm (Fig. 20), 1 μm (Figs 16–18), 2 μm (Figs 13, 14, 15, 19).
FIGURES 7–12. Cocconeis sawensis from Brazil. Sternum valves. 7–11. SEM. 12. TEM. 7, 8 in Additional morphological features of the epiphytic diatom Cocconeis sawensis Al-Handal & Riaux-Gobin (Cocconeidaceae, Bacillariophyta) from a coastal lagoon, Southern Brazil
FIGURES 7–12. Cocconeis sawensis from Brazil. Sternum valves. 7–11. SEM. 12. TEM. 7, 8. Epiphytic on Cladophora sp. 8. Note valve convexity, slightly concave along the axial area. 9, 10. External view showing the narrow sternum. 11. Internal view. Detail of the sternum thickened and raised. 12. Valve. Scale bars = 2 μm (Figs 9, 12), 5 μm (Figs 8, 10, 11), 10 μm (Fig. 7).
FIGURES 21–26. Cocconeis sawensis from Brazil. 21–23, 25 SEM. 24, 26. TEM. 21 in Additional morphological features of the epiphytic diatom Cocconeis sawensis Al-Handal & Riaux-Gobin (Cocconeidaceae, Bacillariophyta) from a coastal lagoon, Southern Brazil
FIGURES 21–26. Cocconeis sawensis from Brazil. 21–23, 25 SEM. 24, 26. TEM. 21. Internal view of the raphe valve showing valvocopula of sternum valve attached. 22, 23, 26. Detail of the short fimbriae, triangle-like. 24. Open valvocopula of sternum valve. 25. Open frustule apices showing sternum valve valvocopula. Scale bars = 1 μm (Figs 22, 23, 25, 26), 2 μm (Fig. 24), 5 μm (Fig. 21).
FIGURES 45–53. Cocconeis sawensis, type material from Iraq. 45–50 SEM. 51–53 TEM. 45, 46 in Additional morphological features of the epiphytic diatom Cocconeis sawensis Al-Handal & Riaux-Gobin (Cocconeidaceae, Bacillariophyta) from a coastal lagoon, Southern Brazil
FIGURES 45–53. Cocconeis sawensis, type material from Iraq. 45–50 SEM. 51–53 TEM. 45, 46. External view of the valve raphe. 47–49. Internal view of the sternum valve. 50. External view of the sternum valve. 51. General view of the sternum valve. 52. Areolae with broken hymens. 53. Detail of the fimbriae of sternum valve valvocopula. Scale bars = 2 μm (Figs 45–47, 50, 51), 5 μm (Figs 48, 49), 0.5 μm (Fig. 52), 1 μm (Fig. 53).
FIGURE 1. Verbascum pollen, SEM micrographs. A−C in Pollen morphology of some Verbascum (Scrophulariaceae) taxa in Turkey
FIGURE 1. Verbascum pollen, SEM micrographs. A−C: V. bithynicum; D−F: V. cariense; G−I: V. nudatum; J−M: V. lasianthum; N−R: V. georgicum; S−V: V. pycnostachyum.
FIGURES 20–24. Placoneis itamoemae, SEM. Fig. 20 in Morphology, ultrastructure and distribution of Placoneis itamoemae sp. nov. (Cymbellaceae) from Brazil
FIGURES 20–24. Placoneis itamoemae, SEM. Fig. 20. Inside view of the valve, scale = 2 µm. Figs 21, 22. External detail of valve apices showing the distal ends of the raphe, scales = 2 µm. Fig. 23. Striae extending to the mantle, scale = 2 µm. Fig. 24. Internal detail of valve apice showing the helictoglossa, scale = 1 µm.
FIGURES 15–19. Placoneis itamoemae, SEM. Fig. 15 in Morphology, ultrastructure and distribution of Placoneis itamoemae sp. nov. (Cymbellaceae) from Brazil
FIGURES 15–19. Placoneis itamoemae, SEM. Fig. 15. Overview of the valve, scale = 5 µm. Figs 16, 17. External view of central area showing the proximal raphe ends and central pores, scale = 1 µm. Figs 18, 19. Internal view of central area showing the proximal raphe ends and central pores, scale = 2 µm and 1 µm, respectively.
FIGURE 40. Radulae. SEM. A–C in A morphological phylogenetic analysis and generic revision of Australian Helicarionidae (Gastropoda: Pulmonata: Stylommatophora), and an assessment of the relationships of the family 2462
FIGURE 40. Radulae. SEM. A–C. Coneuplecta pampini, AM C438751. D–F. Coneuplecta calculosa, AM C377891. G–I. Liardetia doliolum, BPBM 253684. J–L. Lamprocystis guttula, FMNH 174940. Scale bars 10 mm (A–I), 20 mm (J–L).
FIGURE 25. Radulae. SEM. A–C in A morphological phylogenetic analysis and generic revision of Australian Helicarionidae (Gastropoda: Pulmonata: Stylommatophora), and an assessment of the relationships of the family 2462
FIGURE 25. Radulae. SEM. A–C. Malandena suturalis, AM C425085. D–F Westracystis lissus, AM C205297. G–I. Echonitor albumenoidea, SAM D16567. J–L. Tarocystis responsivus, AM C425327. M–O. New Caledonian species NC3, MNHN no registration number. P–R. New Caledonian species NC5, MNHN no registration number. Scale bars 20 mm (A–R).
FIGURE 37. Spermatophores. SEM. A, B. Elisolimax flavescens, NMSA L5563. C, D in A morphological phylogenetic analysis and generic revision of Australian Helicarionidae (Gastropoda: Pulmonata: Stylommatophora), and an assessment of the relationships of the family 2462
FIGURE 37. Spermatophores. SEM. A, B. Elisolimax flavescens, NMSA L5563. C, D. Macrochlamys petrosa, AM C205305. E, F. Orpiella concavum, BMNH no registration number. Scale bars 0.2 mm (D, F), 0.5 mm (B), 1 mm (A, C, E).
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OpenNeuro
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