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19,799 results for “STEM”
Post-fire succession in 1994 Hajdukovich Creek burn: Measurements of aspen and black spruce stem density
This dataset contains measurements of stem density of aspen and black spruce taken during summer 2009 in the 1994 Hajdukovich Creek burn.
Tree regeneration after fire: Delta 1994 burn surveys, pre-fire stem counts and basal areas, for species other than black spruce
Data for this study were collected in 2001 and 2002 by Jill Johnstone (University of Alaska Fairbanks) and Eric Kasischke (University of Maryland). Sites were located within the perimeter of the 1994 burn southeast of Delta Junction Alaska, USA, bordering the Alaska Highway to the North and the Gerstle River to the West. Sites were selected from satellite classifications prepared by Eric Kasischke to represent different levels of burn severity and post-fire vegetation canopy greenness (NDVI). Site selection was constrained by road access, and only areas where all trees had been killed by the fire were selected. At each site, a central point was located in an area of visually homogeneous vegetation. Five parallel transects, each 50 m long, were laid out as follows: 1) the first transect started at the central point and followed a randomly-selected compass direction, 2) two additional transects were established parallel to the first, but at a random distance from the central transect up to 25 m distant. Vegetation was sampled in a 2-m wide belt centered on each transect, and soil samples were made at intervals along the transect line. Vegetation measurements included: a) basal diameters of all pre-fire trees greater than 1.3 m in height, b) counts of all post-fire tree seedlings, and c) basal diameters of tree seedlings and willows, measured in a randomly chosen 5x2 m portion of each transect. General notes were made on visual percent cover of different vegetation growth forms at the site. Destructive measurements of tree seedlings and willows made in 2001 were used to develop allometric equations to predict dry biomass from basal diameter. Measurements of soil organic layer depth were made at 5 m intervals with the use of a spade to excavate small chunks of sod. At one randomly-selected sample point per transect, a 10x10 cm sample of the organic layer was collected for bulk density measurements. Bulk density samples were dried in a 60degC oven for 48 hours and then w
Tree regeneration after fire: Delta 1994 burn surveys, pre-fire stem counts and basal areas, for black spruce
Data for this study were collected in 2001 and 2002 by Jill Johnstone (University of Alaska Fairbanks) and Eric Kasischke (University of Maryland). Sites were located within the perimeter of the 1994 burn southeast of Delta Junction Alaska, USA, bordering the Alaska Highway to the North and the Gerstle River to the West. Sites were selected from satellite classifications prepared by Eric Kasischke to represent different levels of burn severity and post-fire vegetation canopy greenness (NDVI). Site selection was constrained by road access, and only areas where all trees had been killed by the fire were selected. At each site, a central point was located in an area of visually homogeneous vegetation. Five parallel transects, each 50 m long, were laid out as follows: 1) the first transect started at the central point and followed a randomly-selected compass direction, 2) two additional transects were established parallel to the first, but at a random distance from the central transect up to 25 m distant. Vegetation was sampled in a 2-m wide belt centered on each transect, and soil samples were made at intervals along the transect line. Vegetation measurements included: a) basal diameters of all pre-fire trees greater than 1.3 m in height, b) counts of all post-fire tree seedlings, and c) basal diameters of tree seedlings and willows, measured in a randomly chosen 5x2 m portion of each transect. General notes were made on visual percent cover of different vegetation growth forms at the site. Destructive measurements of tree seedlings and willows made in 2001 were used to develop allometric equations to predict dry biomass from basal diameter. Measurements of soil organic layer depth were made at 5 m intervals with the use of a spade to excavate small chunks of sod. At one randomly-selected sample point per transect, a 10x10 cm sample of the organic layer was collected for bulk density measurements. Bulk density samples were dried in a 60degC oven for 48 hours and then w
Stem temperature at watershed 18, cove 218 gradient plot at the Coweeta Hydrologic Laboratory from 1996 to 1999
Tree stem temperature is currently being collected to determine both diurnal and seasonal variation in tree stem temperatures for all species and sizes present at the Coweeta Watershed 18 Cove 218 Gradient Plot. The purpose of the data is to characterize the tree stem temperature regimes as they relate to tree stem respiration. Data will continue to be collected until approximately December 1997. Data will then be subsequently summarized and published.
Hubbard Brook Experimental Forest: Watershed 5 stem origin tracking data
W5 was experimentally whole-tree harvested in the winter of 1983-84. The initial post-harvest surveys were conducted by Jeffrey Hughes in 1984. The species and stem origin (i.e. stem grew from seed, sprouted from a cut stem or was an established stem post-harvest) for all stems in the 1m2 plots were recorded. This data set summarizes the apparent “winners” in both 1984 and 30 years later in 2014 (some evaluated in 2015). There were a total of 13 surveys in these plots that helped clarify the outcomes over time. This data set summarizes from the larger data set. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.
PIE LTER measurements of the two tallest Phragmites australis stems per five meter interval along transects at the Argilla Rd. salt marsh restoration site (Ipswich, MA) and Rough Meadows reference marsh (Rowley, MA – Stackyard Road area).
The file contains measurements of the two tallest Phragmites australis stems per five meter interval along transects at the Argilla Rd. salt marsh restoration site (Ipswich, MA) and Rough Meadows reference marsh (Rowley, MA – Stackyard Road area). The culvert feeding Argilla Marsh was enlarged in late November 1998, thus data from 1999 onward is considered as representing a hydrologically restored marsh. A long term study not directly part of the PIE LTER, but a companion study related to tidal restrictions and hydrological alterations of salt marshes in the Plum Island ecosystem.
Marsh plant species stem counts for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA.
Marsh plant species stem counts for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA. The TIDE project aims to simulate eutrophication on a large scale by the addition of NO3- aiming to reach 70µM concentrations from May to September every year during the growing season. This fertilization of the marsh has been going on at Sweeney Creek since the 2004 growing season through 2012 and at Clubhead Creek in 2005 and from 2009 till 2012. The reference creeks, West and Nelson, have been sampled in pair with the nutrient enriched creeks.
Parramore Island of the Virginia Coast Reserve Permanent Plot Resurvey: Calibration Saltmarsh Stem data 1996
First 3-5 year resurvey of permanent monitoring plots using essentially the same protocol as the intial survey of 1992-1993 except that: 1.) standing biomass of the herbaceous groundcover was added (including for new lower salt marsh plots) using clip plots at the subplot locations; and 2.) an estimate of landcover/habitat class aggregation was conducted surrounding each plot center out to 60m in the four cardinal directions. Extends baseline data useful for estimating landscape-scale vegetative productivity, mortality, and turnover; for establishing pre-disturbance conditions in the case of later stand- or island-wide disturbance; and for assisting in the ground-truthing of landcover and habitat classification using aerial or satellite remote-sensing imagry.
FIG. 7 in Anatomy, affinities, and evolutionary implications of new silicified stems of Sphenophyllum Brongniart, 1828 from the early Carboniferous (Mississippian) of France and Germany
FIG. 7. — Reconstruction of MN911 at the level of branching based on a series of transverse sections. Because only the base of the leaves is well known, their tips are represented in dashed lines. Sections 1-6 correspond to polished surfaces MN911-B1i, MN911-B1s, MN911-B2i, MN911-B2s, MN911-B3i, and MN911- B3s; sections 7 and 8 correspond to slides MN911-A1 and MN911-A2. Artwork by Bernard Terreaux.
FIG. 2 in Anatomy, affinities, and evolutionary implications of new silicified stems of Sphenophyllum Brongniart, 1828 from the early Carboniferous (Mississippian) of France and Germany
FIG. 2. — General aspect of the specimens in transverse section at the same scale. The primary xylem is in dark grey, the secondary xylem – when present – is in black. A, MN201(slide MN201-E2); B, same specimen as A at a level of branching (slide MN201-F1); C, largest axis resulting from the branching of MN911 with two leaf bases (slide MN911-A1); D, same axis as in C at another level with one leaf base and the production of two traces from one arm of the stele (slide MN911-A2); E, smallest axis resulting from the branching of MN911 (slide MN911-A2); F, MN864 showing secondary xylem all around the stele (slide MN864-C1); G, KLC3 showing a small amount of secondary xylem (slide KLC3-C); H, KLC4 showing a small amount of secondary xylem like KLC3 but a much smaller cortex (slide KLC4-B2). Scale bar: 2 mm.
FIG. 1 in Anatomy, affinities, and evolutionary implications of new silicified stems of Sphenophyllum Brongniart, 1828 from the early Carboniferous (Mississippian) of France and Germany
FIG. 1. — Maps showing the location of the localities in Southern France (A) and Thuringia, Germany (B). Detailed maps modified from Galtier et al. 1988 and Meyer-Berthaud & Rowe 1997.
Dataset related to article "TNF-Stimulated Gene-6 Is a Key Regulator in Switching Stemness and Biological Properties of Mesenchymal Stem Cells."
<p>Mesenchymal stem cells (MSCs) are well established to have promising therapeutic properties. TNF-stimulated gene-6 (TSG-6), a potent tissue-protective and anti-inflammatory factor, has been demonstrated to be responsible for a significant part of the tissue-protecting properties mediated by MSCs. Nevertheless, current knowledge about the biological function of TSG-6 in MSCs is limited. Here, we demonstrated that TSG-6 is a crucial factor that influences many functional properties of MSCs. The transcriptomic sequencing analysis of wild-type (WT) and TSG-6<sup>-/-</sup> -MSCs shows that the loss of TSG-6 expression leads to the perturbation of several transcription factors, cytokines, and other key biological pathways. TSG-6<sup>-/-</sup> -MSCs appeared morphologically different with dissimilar cytoskeleton organization, significantly reduced size of extracellular vesicles, decreased cell proliferative rate, and loss of differentiation abilities compared with the WT cells. These cellular effects may be due to TSG-6-mediated changes in the extracellular matrix (ECM) environment. The supplementation of ECM with exogenous TSG-6, in fact, rescued cell proliferation and changes in morphology. Importantly, TSG-6-deficient MSCs displayed an increased capacity to release interleukin-6 conferring pro-inflammatory and pro-tumorigenic properties to the MSCs. Overall, our data provide strong evidence that TSG-6 is crucial for the maintenance of stemness and other biological properties of murine MSCs.</p> <p> </p> <p>Some dataset of this research are in prism format, to ensure open access we attach a pdf instruction about this format and a link where downoladed it</p>
Dataset related to article "The soluble glycoprotein NMB (GPNMB) produced by macrophages induces cancer stemness and metastasis via CD44 and IL-33"
<p>This record contains data related to article “The soluble glycoprotein NMB (GPNMB) produced by macrophages induces cancer stemness and metastasis via CD44 and IL-33"</p> <p> </p> <p>Abstract</p> <p>In cancer, myeloid cells have tumor-supporting roles. We reported that the protein GPNMB (glycoprotein nonmetastatic B) was profoundly upregulated in macrophages interacting with tumor cells. Here, using mouse tumor models, we show that macrophage-derived soluble GPNMB increases tumor growth and metastasis in Gpnmb-mutant mice (DBA/2J). GPNMB triggers in the cancer cells the formation of self-renewing spheroids, which are characterized by the expression of cancer stem cell markers, prolonged cell survival and increased tumor-forming ability. Through the CD44 receptor, GPNMB mechanistically activates tumor cells to express the cytokine IL-33 and its receptor IL-1R1L. We also determined that recombinant IL-33 binding to IL-1R1L is sufficient to induce tumor spheroid formation with features of cancer stem cells. Overall, our results reveal a new paracrine axis, GPNMB and IL-33, which is activated during the cross talk of macrophages with tumor cells and eventually promotes cancer cell survival, the expansion of cancer stem cells and the acquisition of a metastatic phenotype.</p> <p> </p>
Lactate Increases Stemness of CD8+ T Cells to Augment Anti-Tumor Immunity
<p>The immunological role of lactate in antitumor immunity is not well understood. In this study, we report lactate treatment significantly augments antitumor efficacy of immune checkpoint blockade or T cell vaccine therapy in multiple tumor models. Single cell transcriptomics and flow cytometry analysis revealed an increased subpopulation of stem-like TCF-1-expressing CD8<sup>+</sup> T cells upon lactate treatment.</p>
GFP-GOWT1 mouse stem cells TIFF file
<p>This is accompanying file to the CellMAPtracer software (<a href="https://doi.org/10.5281/zenodo.3878087">https://doi.org/10.5281/zenodo.3878087</a>).</p>
Fig. 3. Cucumis thulinianus. A. Flowering stem with leaves, x 3 in Biosystematic monograph of the genus Cucumus (Cucurbitaceae). Botanical identification of cucumbers and melons
Fig. 3. Cucumis thulinianus. A. Flowering stem with leaves, x 3/4. B. Male flower, x 7. C. Male flower, opened to show stamens and disc, x 11. D. Fruit, x 'A. E. Fruit aculeus with retrorse strigose pubescence, x 2. F. Seed, face and side views, x 5. (Bally & Melville 16028 (K), D, E, F; Hemming 2011 (K), A, B, C.)
Fig. 1. Cucumis carolinus. A. Flowering stem with leaves, x V2. B. Male flower, x 1. C in Biosystematic monograph of the genus Cucumus (Cucurbitaceae). Botanical identification of cucumbers and melons
Fig. 1. Cucumis carolinus. A. Flowering stem with leaves, x V2. B. Male flower, x 1. C. Male flower, opened to show stamens and disc, x 5. D. Female flower, x 1. E. Female flower, opened to show disc, style, stigma, and staminodes, x 2*A. F. Fruit, x V2. G. Seed, face and side views, x 5. (Carter & Stannard 697 (K), B, C; Gilbert & Thulin 1116 (K), A, D, E, F, G.)
Fig. 4. Cucumis rostratus. A. Flowering stem with leaves, x Vi. B. Male flower, x 5. C in Biosystematic monograph of the genus Cucumus (Cucurbitaceae). Botanical identification of cucumbers and melons
Fig. 4. Cucumis rostratus. A. Flowering stem with leaves, x Vi. B. Male flower, x 5. C. Male flower, opened to show stamens and disc, x 10. D. Female flower, x 2. E. Fruit, x Vi. F. Seed, face and side views, x 4Vi. (Aké-Assi 5495 (K), E; Brenan 8875 (K), A, B, C, D, F.)
Fig. 2. Cucumis prolatior. A. Flowering stem with leaves, x 16. B. Male flower, x 5. C in Biosystematic monograph of the genus Cucumus (Cucurbitaceae). Botanical identification of cucumbers and melons
Fig. 2. Cucumis prolatior. A. Flowering stem with leaves, x 16. B. Male flower, x 5. C. Male flower, opened to show stamens and disc, x 716. D. Fruit, x 16. E. Seed, face and side views, x 5. {Bally B3645 (K), D, E; Gillett & Gachathi 20478 (K), A, B, C.)
Cryo-4D-STEM datasets on cells and cellular organelles for demonstrating a dose-Efficient cryo-EM technique: tilt-Corrected Scanning Transmission Electron Microscopy
<p>This upload contains three 4D-STEM datasets in .raw format for demonstrating a dose-efficient cryo-EM technique for thick samples: tilt-corrected Scanning Transmission Electron Microscopy (tcBF-STEM). The dataset dimension is 128130256*256. Data were acquired on vitrified intact E.coli cells and isolated human cell organelles. This upload also contains the EFTEM images in .mrc acqired in the same ROI as the 4D-STEM dataset. </p> <p>It also contains analysis of the manuscript's Fig 3 and Ext. data fig 8. </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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