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80 results for “Steinernematidae”
FIGURE 1. Steinernema guangdongense n in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 1. Steinernema guangdongense n. sp, first generation male and infective juvenile. Scale bars: A, B,C = 50 μm, D, E. = 20 μm.
FIGURE 2 in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 2. SEM photographs of the firstgeneration Steinernema guangdongense n. sp. A, head of a young female showing labial (l) and cephalic (c) papillae. B, tail of a young female. C, posterior end of a male showing genital papillae (p). E, F, spicule and gubernaculum. Scale bars: A, D = 10 μm, B = 42.9 μm, C = 42.5 μm. E, F = 20 μm.
FIGURE 9. Phylogenetic relationships between 25 in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 9. Phylogenetic relationships between 25 species of Steinernema with bootstrap analysis of partial 28S sequences. The eight species S. guangdongense n. sp., S. longicaudum (strains CWL05, USA and CB2B) S. glaseri, S. cubanum, S. longicaudum CH, S. arenarium, S. puertoricense and S. karii form a monophyletic group. Numbers at the nodes represent bootstrap proportion.
FIGURE 4. Steinernema guangdongense n in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 4. Steinernema guangdongense n. sp. first and second generations females. A, anterior region. B, C, variation in tail shape and mucronlike structure. D, vulva and double flapped epiptygma. E, vulva and epiptygma of the second generation female. Scale bar: AE = 50 μm.
FIGURE 5. Steinernema guangdongense n in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 5. Steinernema guangdongense n. sp. Lightmicroscope photographs. AB, epiptygma of the first generation females. CD, tails of infective juveniles showing dorsal constriction (arrows), compared to no dorsal constriction in S. longicaudum in EF. G, mature female of the first generation with prominent postanal swelling. H, second generation female with longer tail. Scales: A, B = 18 μm, C = 24 μm, D = 22 μm, E, F = 22 μm.
FIGURE 6 in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 6. SEM photographs of Steinernema guangdongense n. sp. infective juvenile. AB, anterior region showing one lateral line, closed mouth (m), amphids (a) and cephalic (c) papillae. C, lateral field with 2 ridges (3 incisures). D, lateral field showing the change of lateral field pattern from 2 to 7 ridges. E, lateral field showing 7 ridges and the middle one (number 4) is divided into 2 making 8 ridges in lateral field. F, lateral field showing phasmid (p) and 7 ridges changing to 4 then 2. Scales: A = 6.67 μm, B = 5 μm, C = 8.60 μm, D F = 6.67 μm.
FIGURE 4 in Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy
FIGURE 4. RFLP profiles for (A) Steinernema apuliae sp. n., (B) S. arenarium and (C) S. glaseri. M molecular weight markers (band sizes shown in base pairs); Sf, the ITS region from S. feltiae (UK site 76) digested with Alu I; lanes 1 to 17 the ITS region for each isolate digested with the following restriction enzymes; 1, Alu I; 2, BstO I; 3, Dde I; 4, EcoR I; 5, Hae III; 6, Hha I; 7, Hind III; 8, Hinf I; 9, Hpa II; 10, Kpn I; 11, Pst I; 12, Pvu II; 13, Rsa I; 14, Sal I; 15, Sau3A I; 16, Sau96 I; 17, Xba I.
FIGURE 2. A in Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy
FIGURE 2. A, SEM second generation male of S. apuliae sp. n, dorsal view of posterior region of male and 2 pairs of adcloacal papillae (a and b), 3 pairs of papillae near tail tip; B, second generation male, Nomarski LM, esophagus and excretory pore; C, second generation male, Nomarski LM, mail tale with spicules and gubernaculum.
FIGURE 3. A in Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy
FIGURE 3. A, SEM of first generation female of S. apuliae sp. n., head with 6 labial and 4 cephalic papillae; B, first generation female, Nomarski LM, tail with apical papillae or protuberance; C, SEM of first generation female, tail with apical papillae or protuberances; D, SEM of first generation female, lateral view of tail with conicallike tip and apical papilla or protuberance; E, second generation female, Nomarski LM, asymmetrical vulva; F, SEM of second generation female, asymmetrical vulva; G, SEM of second generation female, ventral view of tail with an apical protuberance; H, SEM of infective juvenile, lateral field with 8 identical ridges; I, infective juvenile, Nomarski LM, esophageal portion with excretory pore and hemizonid; L, infective juvenile, Nomarski LM, tail with hyaline portion.
FIGURE 5 in Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China
FIGURE 5. Alignment of the D2D3 domain of 28S rDNA sequence of Steinernema pui sp. n. with those of closely related Steinernema species.
FIGURE 4 in Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China
FIGURE 4. Phylogenetic relationship of Steinernema pui sp. n. with other Steinernema species based on the D2D3 domain of 28S rDNA sequence.
FIGURE 1 in Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China
FIGURE 1. Steinernema pui sp. n. in lateral view. A, D and E: the whole body (A), Spicule (D) and tail (E) of first generation male; B, F and G: the anterior region (B), epiptygma (F) and tail (G) of the first generation female; C and F: the anterior region (C) and tail (F) of the infective juvenile. Scale bar (in µm): A = 320; B = 100; C = 32; D = 32; E = 65; F = 78; G = 28 and H = 90.
FIGURE 3 in Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China
FIGURE 3. SEM micrographs of spicules and gubernacula of Steinernema pui sp. n. A and B: spicules of mature males; C: spicule of a young male; D: spicule tip showing an aperture and an irregular shaped concave; E and F: gubernacula in ventral (E) and lateral view (F). Scale bar (in µm): A = 15; B = 13, C = 12; D = 2.6; E and F = 12.
FIGURE 6 in Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China
FIGURE 6. Phylogenetic relationship of Steinernema pui sp. n. with other Steinernema species based on rDNA sequence of the internal transcribed spacer regions.
FIGURE 2 in Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China
FIGURE 2. SEM micrographs of Steinernema pui sp. n. A: face view of a first generation male showing 6 labial papillae (l), 4 cephalic papillae (c) and 2 amphids (a); B: the tail of a first generation female showing a mucron (arrow) and anus; C: the tail of a first generation male showing the shape (conoid, short without mucron) and the distribution of genital papillae (arrows); D: the head of an ensheathed infective juvenile (IJ) showing 4 cephalic papillae (c) and an amphid (a); E: the middle portion of an IJ showing lateral field pattern; F: the tail of an IJ showing lateral field pattern and phasmids (p). Scale bar (in µm): A = 11; B = 30; C = 25; D = 7; E and F = 8.
FIGURE 7 in Steinernema goweni n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Zulia State, Venezuela
FIGURE 7. Phylogenetic relationships in the "bicornutum" group of Steinernema based on analysis of D2D3 regions of the 28S rDNA. Steinernema scapterisci and S. nepalense were used as outgroup taxa. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (10000 replicates) are shown next to the branches. Branch lengths indicate evolutionary distances and are expressed in the units of number of base differences per site.
FIGURE 6 in Steinernema goweni n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Zulia State, Venezuela
FIGURE 6. Phylogenetic relationships in the "bicornutum" group of Steinernema based on analysis of ITS rDNA regions. Steinernema scapterisci and S. nepalense were used as outgroup taxa. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (10000 replicates) are shown next to the branches. Branch lengths indicate evolutionary distances and are expressed in the units of number of base differences per site.
FIGURE 5 in Steinernema goweni n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Zulia State, Venezuela
FIGURE 5. Scanning electron microscope photography of male of Steinernema goweni n. sp. A) head showing amphids (arrow); B) posterior region showing the genital papillae distribution (vp = ventral papilla); C) posterior region showing the genital papillae distribution showing an extra lateral papilla (#13); D) male terminus showing phasmids (ph) and pore (arrow). Scale bars 10µm.
FIGURE 4 in Steinernema goweni n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Zulia State, Venezuela
FIGURE 4. Scanning electron microscope photography of infective juvenile and female of Steinernema goweni n. sp. A) head of infective juvenile showing the "horn-like" structures on the labial region; B) lateral field arrangement at mid body of infective juvenile; C) tail of infective juvenile showing phasmids (arrow); D) female terminus E) female terminus showing phasmids (arrow). Scale bars 10µm
FIGURE 1 in Steinernema goweni n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Zulia State, Venezuela
FIGURE 1. Male and infective juveniles of Steinernema goweni n. sp. A) male of first generation in toto; B) spicule of first generation male; C) gubernaculum of first generation male in ventral view; D,E,F,G) different gubernacula shapes in second generation males; H) head and pharyngeal region of infective juvenile; I) tail of infective juvenile. Scale bars 20µm
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