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Fig. 1 in Acute mortality in California tiger salamander (Ambystoma californiense) and Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) caused by Ribeiroia ondatrae (Class: Trematoda)

Fig. 1. Gross photographs of ethanol-fixed salamanders in late-stage metamorphosis from a mortality event in the Ellicott Slough National Wildlife Refuge (ESNWR) in Santa Cruz County, California, U.S.A. showing integumentary lesions. (A) California tiger salamander (Ambystoma californiense) exhibiting an extensive roughening of the skin with multifocal ulceration around the eyes, gular fold, and dorsal tail (arrows). (B) Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) with ulceration and crust formation on the gular fold and base of the tail (arrow).

Fig. 10 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 10. Representative photographs of slides with Lyperosomum turdia. Host species, sampling dates and host identification numbers are indicated. All specimens originated from the Czech Republic.

Fig. 5 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 5. Representative photographs of slides with Brachydistomum olssoni, Brachydistomum salebrosum, and Brachydistomum ventricosum. Host species, sampling dates and host identification numbers are indicated. All specimens originated from the Czech Republic.

Fig. 4 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 4. Maximum likelihood analysis of sequences of the ITS2 DNA locus of Dicrocoeliidae. Bootstrap values (n = 1000) are indicated for nodal support. Black circles indicate new sequences. The scale-bar indicates the number of substitutions per nucleotide site.

Fig. 1 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 1. Maximum likelihood analysis of sequences of the CO1 DNA locus of Dicrocoeliidae. Bootstrap values (n = 1000) are indicated for nodal support. Black circles indicate new sequences. The scale-bar indicates the number of substitutions per nucleotide site.

Fig. 3 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 3. Maximum likelihood analysis of sequences of nuclear DNA loci (28S rDNA (A) and 18S rDNA (B)) of Dicrocoeliidae. Bootstrap values (n = 1000) are indicated for nodal support. Black circles indicate new sequences. The scale-bars indicate the number of substitutions per nucleotide site.

Fig. 2 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 2. Maximum likelihood analysis of sequences of the ND1 DNA locus of Dicrocoeliidae. Bootstrap values (n = 1000) are indicated for nodal support. Black circles indicate new sequences. The scale-bar indicates the number of substitutions per nucleotide site.

Fig. 9 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 9. Representative photographs of slides with Lyperosomum petiolatum. Host species, sampling dates and host identification numbers are indicated. All specimens originated from the Czech Republic.

Fig. 7 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 7. Representative photographs of slides with Lyperosomum tenori sp. n. (upper part of the figure) and Lyperosomum hirundinis sp. n. (lower part of the figure). Host species, sampling dates and host identification numbers are indicated. All specimens originated from the Czech Republic. Photographs of L. hirundinis sp. n. are composite photographs merged from multiple images. Note that the seeming differences in forebody shape of L. tenori sp. n. individuals is caused by differences in handling with host birds prior the fixation of the trematodes - L. tenori sp. n. from hosts that were frozen prior the examination are highly susceptible to forebody prolongation.

Fig. 11 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 11. Representative photographs of slides with Stromitrema acrocephali sp. n. and Lutztrema atricapillae. Host species, sampling dates and host identification numbers are indicated. All specimens originated from the Czech Republic.

Fig. 6 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 6. Drawings of holotype specimens of Lyperosomum tenori sp. n. (A), Lyperosomum atricapillae sp. n. (B), Stromitrema acrocephali sp. n. (C), Lutztrema atricapillae sp. n. (D), and Lyperosomum hirundinis sp. n. (E–F).

Fig. 8 in New molecular data help clarify the taxonomy of Central European avian Dicrocoeliidae Looss, 1899 (Trematoda: Plagiorchiida)

Fig. 8. Representative photographs of slides with Lyperosomum atricapillae sp. n. Host species, sampling dates and host identification numbers are indicated. All specimens originated from the Czech Republic.

Fig. 5 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)

Fig. 5. Bayesian inference phylogenetic tree of the Echinostomatoidea superfamily showing the position of trematodes extracted from Snail Kite from Ecuador (in bold). The tree was built using small subunit of the ribosomal RNA gene (18 S rDNA) in Beast v1.10.4. Bayesian posterior probability values ≥ 0.5 are shown in branches. Family, genus, and species from each sequence are listed along with their accession number. Family delimitations are indicated with grey boxes. Sequences of Schinostomatoidea and Opisthorchioidea were used as outgroups. Scale bar indicates number of expected substitutions per site.

Fig. 4 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)

Fig. 4. Trematodes in the right abdominal air sac, serosa of gastrointestinal tract and celomic cavity of a Snail Kite (Rostrhamus sociabilis).

Fig. 2 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)

Fig. 2. Trematodes in respiratory system and pneumatic bone of a Snail Kite (Rostrhamus sociabilis): A. Macroscopic image of the lungs, with presence of trematodes (arrows) found during necropsy. B. Trematodes (arrows) colonising the lumen of a tertiary bronchus (10x H&E) C. Trematodes (arrows) in an abdominal air sac (10x H&E) D. A parasite (arrow) in the coracoid bone (10x H&E).

Fig. 1 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)

Fig. 1. Trematodes in serosa of heart and proventriculus of Snail Kite (Rostrhamus sociabilis): A. Close up image of a trematode (arrow) in the pericardium during necropsy. B. Histologic capture of a trematode (arrow) present in the serosa of the proventriculus (H&E).

Fig. 3 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)

Fig. 3. Trematodes in the liver of a Snail Kite (Rostrhamus sociabilis): A. Trematodes (arrows) in the serosa of proventriculus, gizzard and liver found during postmortem procedure. B. Miracidium (arrow) in the liver parenchyma (40x H&E).

Fig. 6 in Death by massive air sac fluke (Trematoda: Bothriogaster variolaris) infection in a free-ranging snail kite (Rostrhamus sociabilis)

Fig. 6. Full body (A), anterior end (B) and posterior end (C) of trematode Bothrigaster variolaris from a Snail Kite (Rostrhamus sociabilis). T = midbody testes, V = ventral sucker, p = pharynx, O = postesticular ovary, E = eggs.

Fig. 1 in Size does not matter: molecular phylogeny reveals one of the largest trematodes from vertebrates, the enigmatic Ithyoclinostomum dimorphum, as a species of Clinostomum (Trematoda: Clinostomidae)

Fig. 1. Metacercariae of Clinostomum dimorphum found in the erythrinid fish, Hoplias intermedius from Brazil: (A) Whole view of a paragenophore specimen. B) Detail of reproductive structures of a hologenophore specimen.

Fig. 2 in Size does not matter: molecular phylogeny reveals one of the largest trematodes from vertebrates, the enigmatic Ithyoclinostomum dimorphum, as a species of Clinostomum (Trematoda: Clinostomidae)

Fig. 2. Maximum likelihood phylogram based on the concatenated ITS1-5.8S-ITS2 + 28S + cox1 datasets of Clinostomum dimorphum (in bold) and selected species of the family Clinostomidae. Clade formed by isolates of 'Ithyoclinostomum' yamagutii (incertae sedis) is highlighted in grey. Taxon names are followed by GenBank accession numbers of ITS, 28S, and cox1, respectively, and country of record. Branch length scale bar indicates number of substitutions per site. Abbreviations: HON, Honduras; ITA, Italy; KEN, Kenya; MEX, Mexico; THAI, Thailand; USA, United States of America.