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Fig. 5 in A Hotspot of Amoebae Diversity: 8 New Naked Amoebae Associated with the Planktonic Bloom-forming Cyanobacterium Microcystis

Fig. 5. LM pictures of Schoutedamoeba minuta showing trophozoites in non-oriented movement (a–f) and the limax-shaped locomotive form (g–i). The presence of a pronounced hyaline cap, small adhesive uroidal filaments and tiny granules in the cytoplasm are indicated with white arrowheads, black arrows and a white arrow respectively. Scale bars: 20 µm.

Fig. 7. A – 18S in A Hotspot of Amoebae Diversity: 8 New Naked Amoebae Associated with the Planktonic Bloom-forming Cyanobacterium Microcystis

Fig. 7. A – 18S rDNA maximum likelihood phylogeny of Cochliopodium, including the Microcystis-associated strain (in bold). ML bootstrap values respectively posterior probabilities are shown at the nodes. GenBank accession numbers are given together with the species names. B – LM pictures of locomotive amoebae clearly showing the surrounding hyaline sheet (black arrows) punctuated with microscales (best visible in 8), a few small subpseudopodia (black arrowheads) and trailing adhesive uroidal filaments (white arrows) (1–5), a trophozoite during and just after grazing on Microcystis cells showing a prominent fringe of folded hyaloplasm (white arrowheads) (6–9), the bell-shaped form on colonies of Microcystis aeruginosa (10–11) and floating amoebae (12–13). Scale bars: 20 µm.

Fig. 7A–D in Testate Amoebae from South Vietnam Waterbodies with the Description of New Species Difflugia vietnamica sp. nov.

Fig. 7A–D. Scanning electron micrographs of Difflugia vietnamica sp. nov. (A, B) View of shape (A) and view with emphasis on the apertural edge (B) of the first specimen. (C, D) View of shape (C) and view with emphasis on the apertural edge (D) of second specimen.

Fig. 4 in Testate Amoebae from South Vietnam Waterbodies with the Description of New Species Difflugia vietnamica sp. nov.

Fig. 4. Dendrogram of cluster analysis (complete linkage). Rivers (RI), wetlands (WE), natural lakes (NL), reservoirs (RE), irrigation channels (ICH).

Fig. 2 in Testate Amoebae from South Vietnam Waterbodies with the Description of New Species Difflugia vietnamica sp. nov.

Fig. 2. Species richness of testate amoebae within different genera in waterbodies of South Vietnam.

Fig. 5 in Testate Amoebae from South Vietnam Waterbodies with the Description of New Species Difflugia vietnamica sp. nov.

Fig. 5. PCA ordination biplot of testate amoebae samples, species richness (SR) and environmental variables – temperature (Temp), pH, dissolved oxygen (DO), electrical conductivity (EC). Samples were collected from different-type waterbodies: irrigation channels (ICH), natural lakes NL), reservoirs (RE), rivers (RI), wetlands (WE).

Figs 22–27 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Figs 22–27. Ultrastructure of the amoeba of Luapeleamoeba hula n. g. n. sp. 22. General ultrastructure showing nucleus (N) with homogeneous central nucleolus, mitochondria (M), and centrosomal region with Golgi n. g. n. sp. (G) and MTOC. Note lack of an obvious cell coat. Scale bar – 2.0 µm. 23. Detail of nucleus (N) and portion of the nucleolus (Nu). Scale bar – 1.0 µm. 24. Detail of mitochondrion (M). Scale bar – 500 nm. 25. Detail of cell surface showing microfilament-rich cortex and lack of cell coat. Scale bar – 500 nm. 26, 27. Detail centrosomal region, Golgi (G), MTOC, and microtubules (MT on figures). Fig. 26 is an enlargement of the centrosomal region of Fig. 22 and views the MTOC from a perspective that shows its lamellate structure. Scale bars – 200 nm.

Figs 12–21 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Figs 12–21. Time series of events in Luapeleamoeba hula n. g. n. sp. life cycle. 12–15. Changes in spore shape. 16–18. Ingestion of basidiospore. 19–21. Cytokinesis. All images taken with 20 × dry lens bright field microscopy on agar surface in primary isolation plate (PIP). Approximately 30 sec elapsed between images. Scale bars: 10µm throughout.

Fig. 6 in Evidence Supporting the Concept of a Regionalized Distribution of Testate Amoebae in the Arctic

Fig. 6. Distribution map of Centropyxis pontigulasiformis and C. gasparella in the Arctic and subarctic Norway. Remark that C. pontigulasiformis has recently been observed in the Netherlands and in Austria.

Fig. 2 in Chlorophyll-a and Suspended Inorganic Material Affecting the Shell Traits of Testate Amoebae Community

Fig. 2. Ordination results of the non-metric multidimensional scaling to combination between environments and testate amoeba species. Symbols represent the environments with lower (EL), intermediate (EI), and higher (EH) concentration of inorganic suspended material. Species: Cyp – Cyphoderia cf. ampulla, Eu – Euglypha acanthophora, Lm – Lesquereusia mimetica, Lt – L. modesta, Lu – L. modesta var. caudata, Lo – L. ovalis, Ls – L. spiralis, Lh – L. spiralis var. hirsuta, Lc – L. spiralis var. caudata, No – Netzelia oviformis, Nt – N. tuberculata, Nw – N. wailesi, He – Heleopera petricola, Ne – Nebela penardiana, Phr – Phryganella hemisphaerica, Ho – Hoogenraadia criptostoma, Pl – Plagiopyxis callida, Cyc – Cyclopyxis kahli, Tr – Trinema lineare. Arcellidae species (As), Centropyxidae species (Cs), and Difflugiidae species (Ds) were grouped to more easily identify the relationships between species and environments.

Fig. 4 in Evidence Supporting the Concept of a Regionalized Distribution of Testate Amoebae in the Arctic

Fig. 4. Map of the Arctic (polar view) with climatic zonations according to Prik (1981) and the location of the different clustergroup members. The delination of the southern limit of the Arctic is given by the arrow with the number 1. The arrow 2 gives the southern limit of the High Arctic, arrow 3 the southern limit of the Polar Desert zone (according to Aleksandrova 1980). Sv-Gr stands for the suggested SvalbardGreenlandic protozoological region, Si stands for the Siberian.

Fig. 10 in Description of a New Testate Amoebae Genus Meisterfeldia with Notes on the Systematics of the Suborder Phryganellina (Amebozoa; Tubulinea; Arcellinida)

Fig. 10. Schematic demonstration of relationship of Meisterfeldia chibisovi sp. nov., M. wegeneri sp. nov., M. polygonia sp. nov., M. vanhoornei comb. nov. and M. turfacea comb. nov. based on morphological characters (average morphometric characteristics of species based on literature data and personal observations).

Fig. 6 in Description of a New Testate Amoebae Genus Meisterfeldia with Notes on the Systematics of the Suborder Phryganellina (Amebozoa; Tubulinea; Arcellinida)

Fig. 6. Light microscopical image of Meisterfeldia polygonia in lateral view. A – typical specimen, B – morphological variability.

Fig. 7 in Description of a New Testate Amoebae Genus Meisterfeldia with Notes on the Systematics of the Suborder Phryganellina (Amebozoa; Tubulinea; Arcellinida)

Fig. 7. Outline of Meisterfeldia polygonia: lateral (left) and ventral (right) views. 1–4 – characters of the test measured. Scale bar: 5 µm.

Fig. 11 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Fig. 11. Fluorescence image of fixed Luapeleamoeba hula n. g. n. sp. amoebae. Red is actin stain and blue is DNA stain. The amoebae on the left and right are motile, the one in the center is stationary. Small areas of DNA fluorescence outside the nuclei are from undigested bacteria in the food vacuoles. Scale bar 10 µm.

Figs 1–10 in A New Amoeba with Protosteloid Fruiting: Luapeleamoeba hula n. g. n. sp. (Acanthamoebidae, Centramoebida, Amoebozoa)

Figs 1–10. Light micrographs of Luapeleamoeba hula n. g. n. sp. strain LHI05M-5a-1. 1. Side view of fruiting body on native substrate in primary isolation plate (PIP). 2. Living amoeba on agar surface in monoeukaryotic culture. The cell is moving in the direction of the bottom of the image. 3. Amoebae and fruiting bodies in various stages of development on agar surface viewed subaerially from top in a PIP during the late afternoon. i – immature sporocarp, M – mature sporocarp, P – prespore cell. Note the apparent thickness of the amoebae. 4. Fruiting bodies in various stages of development viewed subaerially on native substrate in PIP during late afternoon. Amoebae seen obliquely are obviously dome shaped. 5. Stalk with apophysis viewed from side after spore has discharged. 6. Floating form of a living amoeba in liquid media slightly flattened with cover slip under 63 × oil differential interference contrast microscopy (DIC). 7. Amoeba gently fixed on slide to maintain locomotive form, 40 × dry DIC. 8. Two living amoebae in PIP digesting fungal spores. 9. Amoeba gently fixed on slide to maintain locomotive form showing blunted triangular subpseudopodia extending from broad hyaline lamellipodium, 40 × dry DIC. 10. Three amoebae gently fixed on slide to maintain locomotive form 40 × dry phase contrast. Scale bars: 10 µm throughout.

Fig. 5 in Description of a New Testate Amoebae Genus Meisterfeldia with Notes on the Systematics of the Suborder Phryganellina (Amebozoa; Tubulinea; Arcellinida)

Fig. 5. Outline of Meisterfeldia wegeneri: lateral (left) and ventral (right) views. 1–4 – characters of the test measured. Scale bar: 5 µm.

Fig. 4 in Description of a New Testate Amoebae Genus Meisterfeldia with Notes on the Systematics of the Suborder Phryganellina (Amebozoa; Tubulinea; Arcellinida)

Fig. 4. Light microscopical image of Meisterfeldia wegeneri in lateral view. A – typical specimen, B – morphological variability.

Fig. 3 in Description of a New Testate Amoebae Genus Meisterfeldia with Notes on the Systematics of the Suborder Phryganellina (Amebozoa; Tubulinea; Arcellinida)

Fig. 3. Outline of Meisterfeldia chibisovi: lateral (left) and ventral (right) views. 1–4 – characters of the test measured. Scale bar: 5 µm.

Fig. 7 in Morphological and Morphometric Description of a Novel Shelled Amoeba Arcella gandalfi sp.nov. (Amoebozoa:Arcellinida) fromBrazilianContinental Waters

Fig. 7. Histograms depicting distribution of data for characters analyzed in this study. The black lines represent density curves that fit our data. Aperture height (ah), test diameter (td) and test top diameter (ttd) present a distribution similar to a bimodal distribution, while all the other characters show a nearly normal distribution. Characters tb2 and bw2 are not shown since they are equal to tb1 and bw1, respectively.