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ShareScore release 0.9.0
Dataset results
45 results for “blink”
An Open-Label, Single-Center Study Evaluating the Effect of Systane Balance on Video Ocular Protection Index (OPI) and Blink Patterns in Patients Diagnosed With Dry Eye
ClinicalTrials.gov study NCT01223040. IPD Sharing: Not stated. Countries: 0. Publications: 0.
Eyelid Movement Sensor Device- Blinking Characterizing
ClinicalTrials.gov study NCT02309814. IPD Sharing: Not stated. Countries: 0. Publications: 0.
QST, Blink Reflex and Nociceptive Flexion Reflex in Headache
ClinicalTrials.gov study NCT04585698. IPD Sharing: Not stated. Countries: 0. Publications: 0.
Dataset to submitted manuscript "Imaging Fluorescence Blinking of a Mitochondrial Localization Probe – a Strategy Combining Site-Specificity with Multi-Parametric Sensing of Cellular Microenvironments"
<p><strong>This folder contains all raw data underlying the results presented in a manuscript, submitted to <em>Small</em></strong><strong>, and entitled:</strong></p> <p> </p> <p><strong><em>Imaging Fluorescence Blinking of a Mitochondrial Localization Probe – a Strategy Combining Site-Specificity with Multi-Parametric Sensing of Cellular Microenvironments</em></strong></p> <p> </p> <p><strong>Authored by:</strong></p> <p>Zhixue Du<sup>a</sup><em>, </em>Joachim Piguet<sup>a,+</sup>, Gleb Baryshnikov<sup>b,+</sup>, Johan Tornmalm<sup>a</sup>, Baris Demirbay<sup>a</sup>, Hans Ågren<sup>b</sup>, Jerker Widengren<sup>a,*</sup></p> <p> </p> <p><sup>a</sup> Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova Univ Center 106 91 Stockholm, Sweden</p> <p><sup>b</sup> Royal Institute of Technology (KTH), Dept Theroretical Chemistry and Biology, Albanova Univ Center 106 91 Stockholm, Sweden</p> <p><sup>+</sup> Contributed equally</p> <p>* Corresponding author: Email: <a href="mailto:jwideng@kth.se">jwideng@kth.se</a>, Phone: +46-8-7907813</p> <p> </p> <p><strong>The data files are grouped into the different techniques used to generate them, and refer to the figures/tables in the manuscript where the extracted results are presented. </strong></p> <p> </p> <p><strong>ABSTRACT</strong></p> <p>The local microenvironment of mitochondrial membranes directly influences cellular metabolic states but are difficult to follow locally. Here, we demonstrate a robust and straightforward strategy, transforming the widely used mitochondrial membrane localization fluorophore 10-Nonyl Acridine Orange (NAO) into a multi-functional probe of membrane microenvironments. By monitoring the blinking kinetics of NAO in small unilamellar vesicles, and by computational simulations, we found that NAO exhibits prominent reversible singlet-triplet state transitions and can act as a light-induced Lewis acid forming a red-emissive doublet radical. The resulting blinking kinetics are highly environment sensitive, specifically reflecting local membrane oxygen concentrations, redox conditions, membrane charge, fluidity and lipid compositions, and can also be imaged in live cells, in a spatially resolved manner. They also reflect hydroxyl ion dependent transitions to and from the fluorophore doublet radical, closely coupled to proton transfer events in the membranes, local pH, and two- and three-dimensional buffering properties on and above the membranes. Generally, by the demonstrated blinking imaging strategy existing fluorophore markers can be transformed into multi-parametric microenvironmental sensors. The strategy makes it possible to image local cellular conditions highly relevant to cancer, metabolic and infectious diseases, thereby providing a basis for cellular diagnostics and for fundamental membrane studies.</p>
Dataset to submitted manuscript "Imaging the blinking properties of a mitochondrial fluorophore marker turns it into a multi-functional sensing probe"
<p><strong>This folder contains all raw data underlying the results presented in a manuscript, submitted to Light: Science & Applications, and entitled:</strong></p> <p> </p> <p><strong><em>Imaging the blinking properties of a mitochondrial fluorophore marker turns it into a multi-functional sensing probe</em></strong></p> <p> </p> <p><strong>Authored by:</strong></p> <p>Zhixue Du<sup>a</sup><em>, </em>Joachim Piguet<sup>a,+</sup>, Gleb Baryshnikov<sup>b,+</sup>, Johan Tornmalm<sup>a</sup>, Baris Demirbay<sup>a</sup>, Hans Ågren<sup>b</sup>, Jerker Widengren<sup>a,*</sup></p> <p> </p> <p><sup>a</sup> Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova Univ Center 106 91 Stockholm, Sweden</p> <p><sup>b</sup> Royal Institute of Technology (KTH), Dept Theroretical Chemistry and Biology, Albanova Univ Center 106 91 Stockholm, Sweden</p> <p><sup>+</sup> Contributed equally</p> <p>* Corresponding author: Email: <a href="mailto:jwideng@kth.se">jwideng@kth.se</a>, Phone: +46-8-7907813</p> <p> </p> <p><strong>The data files are grouped into the different techniques used to generate them, and refer to the figures/tables in the manuscript where the extracted results are presented. </strong></p> <p> </p> <p><strong>ABSTRACT</strong></p> <p>By transient state (TRAST) measurements, monitoring the average fluorescence intensity upon excitation modulation, we show that the mitochondrial localization fluorophore 10-Nonyl Acridine Orange (NAO) exhibits prominent singlet-triplet state transitions and can act as a light-induced Lewis acid forming a red-emissive doublet radical. The blinking properties resulting from these transitions can be monitored in a broadly applicable manner, and under biologically relevant conditions. By TRAST studies of NAO in small unilamellar vesicles (SUVs) we show that these blinking properties are highly environment sensitive, specifically reflecting local oxygen concentrations, redox conditions, membrane charge, fluidity and lipid compositions. In SUVs containing the phospholipid cardiolipin (CL) the NAO blinking properties depend not only on the concentration of the CL added, but also on the CL acyl chain composition. The blinking also reflects hydroxyl ion dependent transitions to and from the NAO doublet radical. This makes it possible to monitor local pH and buffering properties, from a 3D bulk buffer above the membrane, or from a 2D buffer at the membrane surface itself. Finally, by live cell TRAST imaging, we show that the fluorescence blinking properties of NAO can also be imaged in a spatially resolved manner, and that this commercially available location-specific fluorophore thereby can be turned into a multi-parametric intracellular sensing probe. This study demonstrates new possibilities for fundamental membrane studies in artificial vesicles and live cells, using existing fluorophore markers, monitoring parameters and conditions of large biological relevance, which are difficult to retrieve by other means.</p>
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