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FIGURE 16. A-C in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 16. A-C. Aphidina mounted in lactophenol gum about 1950 by Heinze, overview (A) and enlargements with cracked and darkened medium (B, C) with crystals (C). D-J. Kinorhyncha mounted in Permount™ between 1964 and 1968 by Higgins; early stage of deterioration with initial cracks (G-I), intermediate stage with incomplete crack coverage (D, J: see also same slide in Fig. 16A), and final stages with entirely cracked and whitish (E: see also same slide in Fig. 16B, C) or yellowish (F: see also Fig. 16D-F; same slide) mounting medium. Arrowheads mark specimens. A, D-F: macro lens; B, G, J: bright field illumination; C, H, I: DIC. Scalebars: A, D-F, 2 cm; B, G, J, 5 mm; C, H, 300 µm; I, 200 µm.

FIGURE 14 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 14. Chemical structure of various chemicals such as clearing agents, macerating agents, plasticizer, and organic solvents used for microscope slide preparation, part 2. Et, ethyl; n-Bu, n-butyl; OBu, butoxy.

FIGURE 13 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 13. Chemical structure of various chemicals such as clearing agents, macerating agents, plasticizer, and organic solvents used for microscope slide preparation, part 1. Me, methyl; Phe, phenyl.

FIGURE 11 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 11. Chemical structure of the more volatile ingredients of Venetian turpentine from Larix decidua. Ac, acetyl.

FIGURE 7 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 7. Chemical structure of the ingredients of sandarac resin from Tetraclinis articulata in Euparal. Me, methyl.

FIGURE 9 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 9. Chemical structure of the potential ingredients of styrax or storax resin from Liquidambar orientalis. Me, methyl.

FIGURE 3 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 3. Small-sized (A-E) or otherwise unusual slides (F-K). Notice bottom (A, arrowheads) or top glass bars (C, F, H), thick cardboard labels (D, G, I, K), label extending beyond glass slide (F, arrowheads mark end of slide), yellowed mounting medium in periphery of coverslips (G), and slide with top glass slide instead of coverslip (J). Coverslip mounted on pieces of glass as spacer (H, arrowheads). K. Trematode mounted between two glass bars (black arrowheads) under coverslip (white arrowheads mark coverslip margins); broken glass slide repaired with the help of a 2nd lower glass slide, cracks of upper slide indicated by black arrows. Specimens mounted in glycerol (now dry) 1868 by von Nathusius (A), in unknown media1888 by Stuhlmann (B), in the 19th century by Krabbe (C), before 1917 by Weltner (D), before 1918 by an unknown person (F), before 1914 by von Linstow (G), before 1918 by Gaffron (H), between 1904 and 1927 by Zelinka (I), between 1906 and 1937 by Wilhelmi (J), and before 1897 by Thoss (K). A-K: macro lens. Scalebars: A-K, 2 cm.

FIGURE 12 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 12. Chemical structure of various synthetic polymers. Bu, butyl; Cl, chlorine; CMC, carboxy methyl cellulose; CN, cellulose nitrate; Et, ethyl; EtOH, hydroxyethyl; HPMC, hydroxypropyl cellulose; MC, methyl cellulose; Me, methyl; OAc, hydroxyacetyl; PBMA, poly(butyl methacrylate); PDMS, poly(dimethyl siloxane); PEMA, poly(ethyl methacrylate); Phe, phenyl; PHEMA, poly(2-hydroxyethyl methacrylate); PMMA, poly(methyl methacrylate); PMMA-PBMA, copolymer of PMMA and PBMA; PS, polystyrene; PVAc, poly(vinyl acetate); PVC, poly(vinyl chloride); PVC-co-PVOAc, copolymer of PVC and PVAc; PVOH, poly(vinyl alcohol); PVP, poly(vinyl pyrrolidone).

FIGURE 5. A in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 5. A. Glycerol-paraffin mount with spacers (arrowheads), probably some kind of modelling clay; made between 2000 and 2007 by Rückert. B, C. Glycerol mounts with metal spacers (B, arrowheads) and cavities because of evaporation of glycerol (C, arrowheads), sealed with a white varnish; early 1920s by Micoletzky. D, E. Pieces of epidermis and cuticle of nematomorphs; early 1930s by Heinze. Notice lack of coverslip (D) and coverslip fastened to slide with adhesive tape (E, arrowheads) and a small amount of a mounting medium. F. Unfinished histological sections still in paraffin, unstained, and without coverslip; early 1920s by Arndt. G. Specimen information inked on glass slide and covered with mounting medium and coverslips; before 1932 by Eckmann. H. Specimen information written with a "permanent" marker on glass side, partly wiped off during cleaning of slide with lab tissue; after 1974 by Kanev. I, J. Slides with self-adhesive labels; glued on back side of slide, and coverslips adhered with mounting medium to aluminum frame (I); mounted in Malinol between 1995 and 2004 by Schmelz (I) and in an unknown medium in 1996 by Erséus (J). K, L. Surface of dry borosilicate coverslip (K) and of wet sodalime glass slide (L) of same slide at same spot (note white crystal in mounting medium marked by asterisk). A-J: macro lens; K, L: DIC. Scalebars: A-J, 2 cm; K, L, 500 µm.

FIGURE 4. A-I in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 4. A-I. Double-coverslip slides with frames of different materials: aluminum (A, Cobb slides), plastic (B, C: Higgins-Shirayama slide with rectangular opening in B and circular opening in C; D, E: aged plastic, coverslip held in place by label paper and detached in D, see arrowheads), thin (F) or thick (G, H) cardboard, and wood (I, coverslip held in place by paper strips). Notice that certain types of plastic and cardboard slides bend upwards centrally (C, E, H) or get easily distorted (D). J. Stains faded and unknown mounting medium yellowed in the periphery of the coverslip. K. Slide with cardboard labels and spacer consisting possibly of coloured paper tissue. Mounted as glycerol-paraffin mounts between 1999 and 2010 by Neuhaus (A), in Hoyer's medium between 2000 and 2001 by Song and Chang (B) and between 1998 and 2004 by Higgins (C), in Canada balsam after 1925 by Schultze or Heider (D, probably E), in gum dammar dissolved in xylene after 1925 by Heider (F-H), and between 1874 and 1890 by Marenzeller (K). A-K: macro lens. Scalebars: A-K, 2 cm.

FIGURE 1 in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 1. Storage of slide collections at the Museum für Naturkunde Berlin (A–E) and National Museum of Natural History, Washington, D.C. (F–I). A–D. Wooden cabinets with drawers and cardboard trays for horizontal storage. Note replacement label for slide on loan (D) and slide mounts of Aphidina between two coverslips previously stored with insect on needle (E, arrows mark holes from needle in cardboard envelopes). F–I. Metal cabinets composed of several units by Technicon (F–H) and Fisherbrand™ (I). G. Metal tray with holes (arrows) for easy access to standard slides from below. Arrowheads mark bentup slide holders. H. Frontal view of opened single unit. I. Dense vertical storage of slides of similar size. Photographs F–I courtesy of Cheryl Bright. D–F: macro lens.

FIGURE 2. A, D in Collection management and study of microscope slides: Storage, profiling, deterioration, restoration procedures, and general recommendations

FIGURE 2. A, D. Cestode (A) and nematodes (D) on oversized blue and white-opaque glass plates, respectively; before 1901 by Borchmann (A) and 19th century by an unknown person (D). Specimen in A without coverslip. B, C, E, G. Histological sections of flatworms (B, E, G) and oligochaete (C) on oversized glass plates; before 1927 by Schulze (B), before 1897 by Michaelsen (C), before 1914 by Böhmig (E), and in the 1980s in DPX in the lab of Sluys (G). Label in C glued and covered with mounting medium. Self-adhesive label in G with glue having migrated through the label indicated by dark spots on the surface of the label. F. Hirudinea on large glass plate with top glass bars; before 1917 by Weltner. Notice yellowed mounting medium in periphery of coverslips in B–F. H. Cestodes on painted pieces of glass; middle of the 19th century by Küchenmeister. A–H: macro lens. Scalebars: A–H, 5 cm.

opencc-zeroJan 2023View details →

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Data for: Chronic corticosterone deteriorates latrine and nesting behaviours in mice

Self-care behaviours are actions that help maintain good health and surroundings. For example, appropriate toileting, sleeping in the bed, and bathing and washing are among self-care behaviours in humans. Animals also perform similar self-care behaviours such as latrine, nesting, and self-grooming. Studies have shown that chronic stress disrupts nesting and self-grooming behaviours. However, the effect of chronic stress on latrine behaviour, preferential, repeated defecation at specific locations, has not yet been clarified. This study aimed to investigate the influence of chronic corticosterone administration on latrine and nesting behaviours in mice. The variation in defecation location was quantified as the degree of the latrine behaviour by using Shannon entropy. The nest quality was scored based on shape. The study showed that mice exposed to chronic corticosterone had scattered defecation sites and lower nest quality compared to the control group. Furthermore, results showed that more scattered defecation behaviour was associated with lower nest quality at an individual level. Additionally, the deterioration of these self-care behaviours was associated with depression-like behaviours such as less open field activity and increased immobility time during the tail suspension test. These results suggest that chronic corticosterone deteriorates self-care behaviours such as latrine and nesting in mice. This dataset includes physiological and behavioural data of mice used in the present study.

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Urban soil quality is being deteriorated even with low heavy metal levels: An arthropod-based multi-indices approach

Urban-induced habitat conversion drastically changes soil life in a variety of ways. Soil sealing, human disturbance, habitat fragmentation, industrial and vehicular pollution are the main causes of urban soil degradation. Soil arthropods, as the most abundant and diverse group of soil fauna, are involved in many soil processes that are of great importance in maintaining soil health and multifunctionality. Nevertheless, soil quality is still mainly characterized by physical, chemical, and microbiological parameters. Here, we assessed and compared the biological soil quality in woody (REF: reference forest, REM: remnant forest) and non-woody (TURF: public turfgrass, and RUD: ruderal habitat) types of urban green spaces along a disturbance and management intensity gradient in the Budapest metropolitan area (Hungary), using community metrics and soil arthropod-based indicators. Vegetation cover and landscape characteristics of study sites were quantified through vegetation and urbanization indices, respectively. Basic soil properties, total and bioavailable concentrations of the main heavy metals (Cd, Co, Hg, Ni, Zn) were also measured. Acari, Collembola, and Hymenoptera (mainly Formicidae) were the most abundant groups. Litter-dweller taxa, particularly Protura, proved to be the most sensitive to urban disturbance. Representatives of Hemiptera, Diptera, Symphyla, and Pauropoda were common in low densities. Soil arthropod assemblages in RUD and TURF were more diverse taxonomically than in REM and REF sites. Although the integrated faunal indices showed no differences among soil habitat types, they provided different responses and, consequently, different information. Our findings demonstrated that the biological quality and arthropod community structure of soils were strongly impacted by soil C/N and heavy metal contamination. We found that low and moderate levels of pollution have adverse effects on edaphic fauna, suggesting biological degradation of soils, even below pollution limits. Nevertheless, more disturbed urban green spaces have been shown to play a significant role in maintaining belowground biodiversity, thereby soil functions.

opencc-zeroMar 2023View details →

Efficacy Argatroban in Ischemic Stroke With Early Deterioration (EASE)

ClinicalTrials.gov study NCT04275180. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Tirofiban for the Prevention of Early Neurological Deterioration After Intravenous Thrombolysis in Acute Ischemic Stroke

ClinicalTrials.gov study NCT06587347. IPD Sharing: UNDECIDED. Countries: 1. Publications: 3.

Qingre Huatan Formula for the Prevention of Early Neurological Deterioration in Acute Ischemic Stroke

ClinicalTrials.gov study NCT06857487. IPD Sharing: Not stated. Countries: 1. Publications: 0.

Clinical Deterioration in Cerebral Venous Thrombosis: A Predictive Study

ClinicalTrials.gov study NCT06266585. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.

closedIPD-NOFeb 2026View details →

Monitoring Neurological Deterioration in Anaesthetised Patients With Electroencephalogram (EEG)

ClinicalTrials.gov study NCT02691338. IPD Sharing: NO. Countries: 1. Publications: 7.