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305 results for “gene clusters”
Sub-functionalization and epigenetic regulation of a biosynthetic gene cluster in Solanaceae
<p>Datasets for the code analysis in <em>Priego-Cubero, Knoch et al., 2024 </em>(https://doi.org/10.1101/2024.10.02.615186)</p>
Data from: Phylogeography of var gene repertoires reveals fine-scale geospatial clustering of Plasmodium falciparum populations in a highly endemic area
Plasmodium falciparum malaria is a major global health problem that is being targeted for progressive elimination. Knowledge of local disease transmission patterns in endemic countries is critical to these elimination efforts. To investigate fine-scale patterns of malaria transmission, we have compared repertoires of rapidly evolving var genes in a highly endemic area. A total of 3680 high quality DBLα sequences were obtained from 68 P. falciparum isolates from ten villages spread over two distinct catchment areas on the north coast of Papua New Guinea (PNG). Modeling of the extent of var gene diversity in the two parasite populations predicts more than twice as many var gene alleles circulating within each catchment (Mugil=906; Wosera=1094) than previously recognized in PNG (Amele=369). In addition, there were limited levels of var gene sharing between populations, consistent with local parasite population structure. Phylogeographic analyses demonstrate that while neutrally evolving microsatellite markers identified population structure only at the catchment level, var gene repertoires reveal further fine-scale geospatial clustering of parasite isolates. The clustering of parasite isolates by village in Mugil, but not in Wosera was consistent with the physical and cultural isolation of the human populations in the two catchments. The study highlights the micro-heterogeneity of P. falciparum transmission in highly endemic areas and demonstrates the potential of var genes as markers of local patterns of parasite population structure.
Long-read genome sequencing accelerated the cloning of Pm69 by resolving the complexity of a rapidly evolving resistance gene cluster in wheat
<p>Oxford Nanopore assembly of <em>Triticum turgidum</em> ssp. <em>dicoccoides, </em>cv. G305-3M.</p>
Systematic Gene Expression Mapping Clusters Nuclear Receptors According to Their Function in the Brain - Website save
<p>This is a copy of the website that was related to mousepat.ics-mci.fr</p>
Microscopy data (1/2): interaction of the gene iscub with RNA polymerase II clusters in inhibitor-treated zebrafish embryos
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>iscub</em> and is obtained from fixed zebrafish embryos, collected at the sphere stage of development. Embryos were treated prior to collection with control media, flavopiridol (10 µM, 30 minutes), JQ-1 (10 µM, 30 minutes), or hexanediol (3% weight/volume, 5 minutes). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two samples were prepared per inhibitor condition, and images were recorded from 3-4 embryos per sample, as indicated in the file names.</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data (2/3): interaction of the gene foxd5 with RNA polymerase II clusters in inhibitor-treated zebrafish embryos
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>foxd5</em> and is obtained from fixed zebrafish embryos, collected at the sphere stage of development. Embryos were treated prior to collection with control media, flavopiridol (10 µM, 30 minutes), JQ-1 (10 µM, 30 minutes), or hexanediol (3% weight/volume, 5 minutes). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two samples were prepared per inhibitor condition, and images were recorded from 3-4 embryos per sample, as indicated in the file names.</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data (3/3): interaction of the gene foxd5 with RNA polymerase II clusters in inhibitor-treated zebrafish embryos
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>foxd5</em> and is obtained from fixed zebrafish embryos, collected at the sphere stage of development. Embryos were treated prior to collection with control media, flavopiridol (10 µM, 30 minutes), JQ-1 (10 µM, 30 minutes), or hexanediol (3% weight/volume, 5 minutes). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two samples were prepared per inhibitor condition, and images were recorded from 3-4 embryos per sample, as indicated in the file names.</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data (1/3): interaction of the gene foxd5 with RNA polymerase II clusters in inhibitor-treated zebrafish embryos
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>foxd5</em> and is obtained from fixed zebrafish embryos, collected at the sphere stage of development. Embryos were treated prior to collection with control media, flavopiridol (10 µM, 30 minutes), JQ-1 (10 µM, 30 minutes), or hexanediol (3% weight/volume, 5 minutes). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two samples were prepared per inhibitor condition, and images were recorded from 3-4 embryos per sample, as indicated in the file names.</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data (2/2): interaction of the gene iscub with RNA polymerase II clusters in inhibitor-treated zebrafish embryos
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>iscub</em> and is obtained from fixed zebrafish embryos, collected at the sphere stage of development. Embryos were treated for 30 minutes prior to collection with control media, flavopiridol (10 µM, 30 minutes), JQ-1 (10 µM, 30 minutes), or hexanediol (3% weight/volume, 5 minutes). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two samples were prepared per inhibitor condition, and images were recorded from 3-4 embryos per sample, as indicated in the file names.</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Intermediary and supplemental data for publication "Heterogenous circulating miRNA changes in ME/CFS converge on a unified cluster of target genes and may be a result of modulation by latent herpesviruses: A computational analysis"
<p>Intermediary and supplemental data for publication "Heterogenous circulating miRNA changes in ME/CFS converge on a unified cluster of target genes and may be a result of modulation by latent herpesviruses: A computational analysis"</p>
Data from: Patterns of genetic diversity and differentiation in resistance gene clusters of two hybridizing European Populus species
Open the record for dataset details and reuse information.
Data from: Comprehensive identification and clustering of CLV3/ESR-related (CLE) genes in plants finds groups with potentially shared function
Open the record for dataset details and reuse information.
Data from: Phylogeography of var gene repertoires reveals fine-scale geospatial clustering of Plasmodium falciparum populations in a highly endemic area
Open the record for dataset details and reuse information.
Data from: A universal probe set for targeted sequencing of 353 nuclear genes from any flowering plant designed using k-medoids clustering
Open the record for dataset details and reuse information.
Clusterization of the normalized gene expression data
<p>Clusterization of the normalized gene expression data</p>
Diversity of biosynthetic gene clusters in gut bacteria of turtle ants
<p class="Standard"><span>In insect-microbe nutritional symbioses the gut symbionts supplement the host diet with nutrients by producing amino acids and vitamins or by degrading lignin or polysaccharides macromolecules. In multipartite mutualisms composed of multiple symbionts from different taxonomical orders, it has been suggested that beside the genes involved in the nutritional symbiosis the symbionts maintain genes responsible for the production of metabolites putatively playing a role in the maintenance and interaction of the bacterial communities living in close proximity. To test this hypothesis, we investigated the diversity of biosynthetic gene clusters (BGCs) producing different non-primary metabolites in the genomes and metagenomes of the conserved gut symbionts associated with the herbivorous turtle ants (genus: <i>Cephalotes</i>). We studied 17 <i>Cephalotes</i> species collected across several geographical areas to reveal that (i) mining metagenomes and genomes show complementary results demonstrating the robustness of this approach to retrieve BGCs, (ii) the conserved gut symbionts involved in the nutritional symbiosis have a high diversity of BGCs of different chemical families, (iii) the phylogenetic analysis of BGCs encoding the production of arylpolyenes, non-ribosomal peptides (NRP), polyketides (PK), and siderophores shows high similarity between BGCs of a single symbiont across different ant host species, and between BGCs originated from different bacterial orders within a single host species. These findings together suggest multiple mechanisms of bacterial genome conservation and evolution of BGCs. We document the occurrence, diversity, and similarity of BGCs in the genome of obligate gut bacteria involved in multipartite mutualisms across the phylogeny of turtle ants.</span></p>
Data from: Islands and streams: clusters and gene flow in wild barley populations from the Levant
The domestication of plants frequently results in a high level of genetic differentiation between domesticated plants and their wild progenitors. This process is counteracted by gene flow between wild and domesticated plants because they are usually able to inter-mate and to exchange genes. We investigated the extent of gene flow between wild barley Hordeum spontaneum and cultivated barley Hordeum vulgare, and its effect on population structure in wild barley by analyzing a collection of 896 wild barley accessions (Barley1K) from Israel and all available Israeli H. vulgare accessions from the Israeli gene bank. We compared the performance of simple sequence repeats (SSR) and single nucleotide polymorphisms (SNP) marker data genotyped over a core collection in estimating population parameters. Estimates of gene flow rates with SSR markers indicated a high level of introgression from cultivated barley into wild barley. After removing accessions from the wild barley sample that were recently admixed with cultivated barley, the inference of population structure improved significantly. Both SSR and SNP markers showed that the genetic population structure of wild barley in Israel corresponds to the three major ecogeographic regions: the coast, the Mediterranean north, and the deserts in the Jordan valley and the South. Gene flow rates were estimated to be higher from north to south than in the opposite direction. As has been observed in other crop species, there is a significant exchange of alleles between the wild species and domesticated varieties that needs to be accounted for in the population genetic analysis of domestication.
Data from: An evolutionary history of the selectin gene cluster in humans
Molecules involved in leukocyte trafficking have a central role in the development of inflammatory and immune responses. We performed FST analysis of the selectin cluster, as well as of SELPLG, ICAM1 and VCAM1. Peaks of significantly high population genetic differentiation were restricted to two regions in SELP and one in SELPLG. Resequencing data indicated that the region covering SELP exons 11–13 displays high nucleotide diversity in Africans and Europeans (CEU), and a high level of within-species diversity compared with inter-specific divergence. Analysis of inferred haplotypes revealed a complex phylogeny with two deeply separated clades that coalesce at ~3.5 million years (MY) plus a minor clade with a TMRCA (time to the most recent common ancestor) of ~2.2 MY. A splicing assay indicated no haplotype-specific effect on SELP exon 14 inclusion. These data are consistent with a model of multiallelic balancing selection; single-nucleotide polymorphism analysis indicated that the Val640Leu variant represents a likely selection target. In populations of Asian ancestry a distinct haplotype, possibly carrying regulatory variants, has been driven to high frequency by positive selection. No deviation from neutrality was observed for the SELPLG region. Resequencing of SELP in chimpanzees revealed a haplotype phylogeny with extremely deep basal branches, suggesting either long-standing balancing selection or ancestral population structure. Thus, SELP has experienced a complex selective history, possibly as a result of local adaptation. Variants in the gene have been associated with autoimmune and cardiovascular diseases. Association studies would benefit from both taking the complex SELP haplotype structure into account and from analysis of possible regulatory variants in the gene.
Data from: Evolutionary ecology of beta-lactam gene clusters in animals
Beta-lactam biosynthesis was thought to occur only in fungi and bacteria, but we recently reported the presence of isopenicillin N synthase in a soil-dwelling animal, Folsomia candida. However, it has remained unclear whether this gene is part of a larger beta-lactam biosynthesis pathway and how widespread the occurrence of penicillin biosynthesis is among animals. Here, we analyzed the distribution of beta-lactam biosynthesis genes throughout the animal kingdom and identified a beta-lactam gene cluster in the genome of F. candida (Collembola), consisting of isopenicillin N synthase (IPNS), δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine synthetase (ACVS), and two cephamycin C genes (cmcI and cmcJ) on a genomic scaffold of 0.76 Mb. All genes are transcriptionally active and are inducible by stress (heat shock). A beta-lactam compound was detected in vivo using an ELISA beta-lactam assay. The gene cluster also contains an ABC transporter which is co-regulated with IPNS and ACVS after heat shock. Furthermore, we show that different combinations of beta-lactam biosynthesis genes are present in over 60% of springtail families but they are absent from genome- and transcript libraries of other animals including close relatives of springtails (Protura, Diplura, and insects). The presence of beta-lactam genes is strongly correlated with an eudaphic (soil-living) lifestyle. Beta-lactam genes IPNS and ACVS each form a phylogenetic clade in between bacteria and fungi, while cmcI and cmcJ genes cluster within bacteria. This suggests a single horizontal gene transfer event most probably from a bacterial host, followed by differential loss in more recently evolving species.
Supplementary Material for the paper entitled "Scalable nonparametric clustering with unified marker gene selection for single-cell RNA-seq data"
<p>This repo contain supplementary tables from the manuscript entitled: "Scalable nonparametric clustering with unified marker gene selection for single-cell RNA-seq data". Clustering is a common way to identify cell types in single-cell RNA-sequencing (scRNA-seq) data. Unfortunately, current methods (i) require users to make human-in-the-loop decisions, which adds significant runtime to bioinformatic analyses, and (ii) reuse the same data twice when testing for differentially expressed genes, which can lead to an increased number of false discoveries. In this work, we overcome these limitations with NCLUSION: a Bayesian nonparametric method that simultaneously clusters cells and selects marker genes. NCLUSION operates without user-defined heuristics to set model parameters and leverages variational expectation-maximization (EM) for posterior inference which allows it to scale well up to 1 million cells. By analyzing publicly available datasets, we illustrate that NCLUSION matches the state-of-the-art clustering performance of competing approaches, achieves improved computational efficiency, and directly enables identification of biologically relevant gene sets driving cluster definitions.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.