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Extracts from Pennebaker et al., 2007, LIWC Manual
Open the record for dataset details and reuse information.
Extracts from Pennebaker et al., 2001, LIWC Manual
Open the record for dataset details and reuse information.
Manual quantification of peroxisome counts in yeast from 2-channel fluorescence Z-stacks
<p>This dataset contains fluorescence microscopy imaging data from various strains of <em>Saccharomyces cerevisiae</em>. The images were used to test software called <em>perox-per-cell,</em> which automatically quantifies peroxisome features in yeast cells based on microscopy data. There are 44 imaging instances in the dataset, each consisting of two Z-stacks, one capturing signal from calcofluor white to identify cell boundaries (blue channel), and one capturing signal from GFP tagged with peroxisome targeting sequence 1 (PTS1) to locate peroxisomes (green channel). These raw microscopy imaging sets are provided as ZVI files in <strong>Zstacks.zip</strong>.</p> <p>We compared <em>perox-per-cell</em>'s automatically-generated peroxisome counts to those derived manually by two individuals. For manual counting, images were deconvolved with theoretically generated point spread functions using Axiovision software V4.9.1 SP2 followed by the generation of maximum intensity Z-projections (MIP) of both blue and green channels. All the deconvolved MIP images from WT and mutant strains were blinded and labelled as ‘1-44’, and their grey levels were set to ‘best fit’ in the Axiovision software prior to providing them to two individuals who manually counted peroxisomes in cells using the ‘measure events’ tool in Axiovision. The maximum intensity projection images used for manual counting are provided as ZVI files in <strong>MaxIntensityProjections.zip</strong>.</p> <p>Each individual's manual counts are included in this dataset within the CSV file <strong>ManualPeroxisomeCounts.csv</strong>. Please note that cell IDs in this file are only indicative of the order in which each individual counted peroxisomes, they do not indicate a specific cell within an image. For example, "Cell5" in Image 3 that was processed by manual counter 1 may not be the same cell as "Cell5" in Image3 processed by manual counter 2. These two entries have the same cell ID only because for both manual counters, they were the 5th cell counted.</p> <p>For our software test, we used wild-type (WT) yeast strains as well as several mutant strains with known peroxisomal defects. The strains used for each image are indicated in the<em> </em><strong>ImageAndStrainTable.csv</strong> file.</p> <p>Experimental details: <em>Saccharomyces cerevisiae</em> cells were grown in synthetic defined medium (SD: 6.7 g/L Yeast nitrogen base without amino acids + 0.79 g/L CSM) with 2% Dextrose in flask cultures shaken at 250 rpm at 30 °C until log phase after which they were pelleted and resuspended in 50 µg/ml calcofluor white stain (Sigma, Cat No. 18909) for 5-10 min followed by imaging at room temperature. 3D images consisting of 26 XY images with a Z-slice spacing of 0.204 µm (total Z-stack thickness 5.1 µm) were acquired at 100× magnification using a fluorescence microscope (Axioskop 2 MOT plus, Carl Zeiss, Inc.) equipped with a Plan Apochromat 100×/1.4 Oil DIC objective, an Axio Cam HRm camera and an HBO 100 Mercury lamp. Identical exposure times (50 ms) were used to acquire the green channel images whereas the exposure time for blue channel was adjusted for individual images based on the intensity of calcofluor staining. </p> <p> </p> <p> </p>
Extracts from Pennebaker et al., 2015, LIWC Manual
Open the record for dataset details and reuse information.
Figure 12 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 12. Right manual ungual phalanx of digit I in A,C, ventral, and B,D, lateral views. A-B, Megaraptor; C-D, Australovenator and schematic representation in E,G, ventral, and F,H, lateral views. E-F, Megaraptor; G-H, Australovenator. Scale bar: 2 cm. Abbreviations: ff, flexor facets.
Figure 10. Right manual phalanx 1 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 10. Right manual phalanx 1 of digit I in ventral view and schematic representations of Megaraptor (A, C), Australovenator (B,D). Scale bar: 2 cm. Note the well-developed longitudinal ventral furrow.
Figure 9 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 9. Proximal end of right phalanx I.1 of A, Megaraptor; B, Australovenator; C, Allosaurus; D, Tyrannosaurus (modified from Brochu, 2003); and E, Deinonychus (modified from Ostrom, 1969). Not to scale.
Figure 6 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 6. Left manus in dorsal view of A, Dilophosaurus (modified from Welles, 1980); B, Allosaurus; C, Megaraptor; D, Sinocalliopteryx; E, Tanycolagreus (modified from Carpenter et al., 2005); F, Deinonychus (modified from Ostrom, 1969); G, Scipionyx (modified from Dal Sasso and Maganuco, 2011); H, Guanlong (modified from Xu et al., 2009); and I, Sinosauropteryx (modified from Currie and Chen, 2001). Not to scale.
Figure 11 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 11. Right manual ungual phalanx of digit I in ventral view and schematic representation of Megaraptor (A,C); and Australovenator (B,D). Not to scale.
Figure 8. A-C in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 8. A-C, left first metacarpal in dorsal view of A, Megaraptor, B, Australovenator, and C, Rapator; D-F, proximal view of left metacarpus of D, Guanlong (modified from Xu et al.,2009), E, Tanycolagreus (modified from Carpenter et al., 2005), and F, Deinonychus (modified from Ostrom, 1969); G-H, proximal view of right first metacarpal of G, Rapator, and H, Australovenator. Not to scale. Abbreviations: pdp, proximomedial process; vpI, ventral process of metacarpal I; vpII, ventral process of metacarpal II.
Figure 7. Right metacarpals II and I in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 7. Right metacarpals II and I in dorsal view of A, Acrocanthosaurus (modified from Currie and Carpenter, 2000); B, Torvosaurus (modified from Galton and Jensen, 1979); C, Megaraptor; D, Deinonychus (modified from Ostrom, 1969); E, Guanlong (modified from Xu et al., 2009). Not to scale. Abbreviations: ep, extensor pit; pdp, proximomedial process; ps, proximolateral surface.
Figure 5 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 5. Left "semilunate" carpal in proximal (upper row) and dorsal (lower row) of A, Allosaurus, B, Acrocanthosaurus (modified from Currie and Carpenter, 2000); C, Megaraptor; D, Guanlong (modified from Xu et al., 2014); E, Ornitholestes (mofiied from Carpenter et al., 2005); F, Tanycolagreus (modified from Carpenter et al., 2005); G, Alxasaurus (modified from Xu et al., 2014); H, Deinonychus (modified from Ostrom, 1969); and I, Australovenator (modified from White et al., 2012). Not to scale. Abbreviations: ag, anterior groove; dp, distal projections.
Figure 3 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 3. Left manus of Megaraptor namunhuaiquii (MUCPv 341) in dorsal view (A) and schematicrepresentation (B). Scale bar: 1 cm.
Figure 4 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 4. Left manus of (A,C), Allosaurus fragilis, and (B,D), Australovenator wintonensis in (A,B) dorsal, and (C,D) ventral views. Not to scale. B,D, mofied from White et al. (2012).
Figure 2 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 2. Distal end of humerus in anterior (A,C,E,G,I,K,) and distal (B,D,F,H,J,L) views of Australovenator (A,B), Allosaurus (C,D), Xuanhanosaurus (E,F), Chilantaisaurus (G,H), Guanlong (I,J), and Coelurus (K,L). Not to scale. A,B, modified from White et al. (2012). G,H, modified from Benson and Xu (2008).
Figure 1 in Phylogenetic relationships of the Cretaceous Gondwanan theropods Megaraptor and Australovenator: the evidence afforded by their manual anatomy
Figure 1. Humerus in lateral (C-I) and medial (A-B,J) views of: A, Megaraptor (MUCPv 341), B, Australovenator, C, Allosaurus, D, Acrocanthosaurus, E, Coelurus, F, Ornitholestes, G, Xuanhanosaurus, H, Torvosaurus, and I, Baryonyx. J, Fukuiraptor. B, modified from White et al. (2012). D, modified from Currie and Carpenter (2000). H, modified from Galton and Jensen (1979). I, modified from Charing and Milner (1997). Scale bar: 5cm. Abbreviations: it, internal tuberosity; lf, longitudinal furrow.
Manual de boas-vindas aos estagiários de enfermagem em um novo setor
<p><span>A partir da perspectiva das acadêmicas e dos profissionais que recebem o estudante no setor, foi possível elaborar o presente manual com o objetivo de instruir a equipe para o recebimento e adequação dos novos estagiários.</span></p>
Fig. 23. Left manual digit II in The Osteology Of Balaur Bondoc, An Island-Dwelling Dromaeosaurid (Dinosauria: Theropoda) From The Late Cretaceous Of Romania
Fig. 23. Left manual digit II of Balaur bondoc (EME PV.313). Left phalanx II-1 (A–F), left phalanx II- 2 (G–L), and left phalanx II-3 (M–N) in extensor (A, G), flexor (B, H), lateral (C, I, M), medial (D, J, N), proximal (E, K), and distal (F, L) views. Abbreviations: ygr, proximal bifurcations of the Y-shaped groove. Scale bar equals 1 cm.
Fig. 22. Right manual digit I in The Osteology Of Balaur Bondoc, An Island-Dwelling Dromaeosaurid (Dinosauria: Theropoda) From The Late Cretaceous Of Romania
Fig. 22. Right manual digit I of Balaur bondoc (EME PV.313). Right phalanx I-1 (A–F) and right phalanx I-2 (G–H) in extensor (A), flexor (B), lateral (C, H), medial (D, G), proximal (E), and distal (F) views. Abbreviations: for, foramen; ft, flexor tubercle. Scale bar equals 1 cm.
Fig. 21. Left manual digit I in The Osteology Of Balaur Bondoc, An Island-Dwelling Dromaeosaurid (Dinosauria: Theropoda) From The Late Cretaceous Of Romania
Fig. 21. Left manual digit I of Balaur bondoc (EME PV.313). Left phalanx I-1 (A–F) and left phalanx I- 2 (G–H) in extensor (A), flexor (B), lateral (C, G), medial (D, H), proximal (E), and distal (F) views. Abbreviations: for, foramen; ft, flexor tubercle; gr, groove separating flexor tubercle from ventral surface of ungual. Scale bar equals 1 cm.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.