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opencc-by-4.0Aug 2020View details →

Fig. 3 in Diversity of trypanosomes in wildlife of the Kafue ecosystem, Zambia

Fig. 3. The sequences of SRA-PCR–positive samples from buffalo, sable antelope, and vervet monkey are determined and the deduced amino acid were aligned with deposited representative sequences from Zambia, Kenya, and Uganda.

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Fig. 2. ITS1 in Diversity of trypanosomes in wildlife of the Kafue ecosystem, Zambia

Fig. 2. ITS1-PCR Gel analysis Gel image of the ITS-PCR-positive samples. The species were estimated by the band size. The expected sizes for each species are T. godfreyi: 220 bp, T. simiae: 331–343 bp, T. theileri: 269–350 bp, T. brucei: 415–431 bp, T. congolense: 560–705 bp (Gaithuma et al., 2019).

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Fig. 1 in Diversity of trypanosomes in wildlife of the Kafue ecosystem, Zambia

Fig. 1. The Kafue ecosystem comprising of the KNP and surrounding GMAs. The black spots indicate sampling points and the red spots indicate areas where rHAT was detected. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4 in The neglected diversity: Description and molecular characterisation of Trypanosoma haploblephari Yeld and Smit, 2006 from endemic catsharks (Scyliorhinidae) in South Africa, the first trypanosome sequence data from sharks globally

Fig. 4. Bayesian Inference (BI)/Maximum Likelihood (ML) analysis showing the phylogenetic position of Trypanosoma haploblephari (Yeld and Smit, 2006) genotypes representing morphotypes A and B inferred from partial 18S rRNA gene sequences. Comparative sequences representing known Trypanosoma species, with Trypanosoma avium (KT728402) as outgroup, were obtained from GenBank. Tree topologies for both the BI and ML trees were identical; the nodal support values (BI/ML) are represented on the BI tree. Some branches have been shortened with each //= 0.04 substitutions per site.

Fig. 3 in The neglected diversity: Description and molecular characterisation of Trypanosoma haploblephari Yeld and Smit, 2006 from endemic catsharks (Scyliorhinidae) in South Africa, the first trypanosome sequence data from sharks globally

Fig. 3. Micrographs of Trypanosoma haploblephari (Yeld and Smit, 2006) morphotype A (A–C) and T. haploblephari morphotype B (D–F) in Giemsa-stained blood films of Haploblepharus pictus and Poroderma pantherinum, respectively. Blood stage with kinetoplast (k) and undulating membrane (μm) visible (A–C); slender forms (B, E); presence of a flagellum (f) in deeply stained individuals (C, F). Scale bar: 10 μm.

Fig. 2 in The neglected diversity: Description and molecular characterisation of Trypanosoma haploblephari Yeld and Smit, 2006 from endemic catsharks (Scyliorhinidae) in South Africa, the first trypanosome sequence data from sharks globally

Fig. 2. Line drawing of Trypanosoma haploblephari (Yeld and Smit, 2006) from the host Poroderma pantherinum (Slide HE18-18) next to a drawing of a red blood cell.

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Fig. 1. The trypanosomes from European moose. A, B in Molecular identification of Trypanosoma theileri complex in Eurasian moose Alces alces (L.)

Fig. 1. The trypanosomes from European moose. A, B. light microscope images; C. drawing scheme. Scale bar 10 μm.

opencc-by-4.0Feb 2020View details →

Distinct roles for H4 and H2A.Z acetylation in RNA transcription in African trypanosomes

This repository contains the run scripts for this publication. Complete data download http://resources-molpara.vetmed.lmu.de/Kraus_et_al_2019_sequencing_analysis.tar.gz Contents of the tar.gz archive: upload_sequencing/ ├── bin/ │   ├── scripts necessary to run the pipeline ├── input/ │   ├── input files ├── output/ │   ├── output files for ChIP and RNA-seq └── output2/     └── output files for ATAC-seq Abstract Despite histone H2A variants and acetylation of histones occurring in almost every eukaryotic organism, it has been difficult to establish direct functional links between canonical histone or H2A variant acetylation, deposition of H2A variants and transcription. To disentangle these complex interdependent processes, we devised a highly sensitive strategy for quantifying histone acetylation levels at specific genomic loci. Taking advantage of the unusual genome organization in Trypanosoma brucei, we identify 58 histone modifications enriched at transcription start sites (TSSs). Furthermore, we find TSS-associated H4 and H2A.Z acetylation to be mediated by two different histone acetyltransferases, HAT2 and HAT1, respectively. Whereas depletion of HAT2 decreases H2A.Z deposition and shifts the site of transcription initiation, depletion of HAT1 does not affect H2A.Z deposition but reduces total mRNA levels by 50%. Thus, specifically reducing H4 or H2A.Z acetylation levels enabled us to reveal distinct roles for these modifications in H2A.Z deposition and RNA transcription.

opencc-by-4.0Dec 2013View details →

Fig. 1 in Trypanosomes genetic diversity, polyparasitism and the population decline of the critically endangered Australian marsupial, the brush tailed bettong or woylie (Bettongia penicillata)

Fig. 1. Geographical origin of the different species of marsupials trapped in this study.

Fig. 1 in The neglected diversity: Description and molecular characterisation of Trypanosoma haploblephari Yeld and Smit, 2006 from endemic catsharks (Scyliorhinidae) in South Africa, the first trypanosome sequence data from sharks globally

Fig. 1. Map of sampling sites on the south coast of South Africa.