Find research datasets worth reusing

opencc-by-4.0Apr 2024View details →

Bacterial Community Weighted rrn Operon Copy Numbers and Enzyme Activity in Costa Rica and Alaska Soils.

Datasets of bacterial community weighted rrn operon copy number and enzyme activity in Alaska and Costa Rica soils. The Alaska soils were collected from across four elevational terraces in a tidal wetland on the western coast. The Costa Rica soils were collected from plots subjected to precipitation manipulation treatments in La Selva Biological Research Station.  The variable descriptions for the Alaska dataset are as follows: simple_id: an identifier variable. id: an identifier variable that includes terrace identity. transect_id: a variable that identifies collection transect location. BG_umol_g_h: the activity rate of beta-glucosiadase in micromoles per gram soil per hour. NAG_umol_g_h: the activity rate of N-acetyl-glucosaminidase in micromoles per gram soil per hour. LAP_umol_g_h: activity rate of leucine-aminopeptidase in micromoles per grams soil per hour. AP_umol_g_h: the activity rate of acid phosphatase in micromoles per gram soil per hour. BG_g_C: the activity rate of beta-glucosiadase in micromoles per gram soil carbon per hour. NAG-g_N: activity rate of N-acetyl-glucosaminidase in micromoles per gram soil nitrogen per hour. LAP_g_N: activity rate of leucine-aminopeptidase in micromoles per gram soil nitrogen per hour. AP_mg_P: activity rate of acid-phosphatase in micromoles per gram soil phosphorus per hour. P_mg_kg: soil phosphorus concentration in milligrams phosphorus per kilogram soil. K_mg_kg: soil potassium concentration in milligrams potassium per kilogram soil. C_pct: soil carbon concentration in percent. N_pct: soil nitrogen concentration in percent. pH: soil pH. cn_ratio: the carbon-to-nitrogen ratio of soil. cp_ratio: the carbon-to-phosphorus ratio of soil. np_ratio: the nitrogen to phosphorus ratio of soil. weighted_copy_number: the bacterial community weighted rrn operon copy number. The variable descriptions for the Costa Rica dataset are as follows: ID: a variable that identifies the soil core collected. Plot: a variable that identifies the experimental plot from which soils were collected. Precip: a variable that describes the precipitation manipulation treatment applied. Core: a variable that identifies whether soil cores were enclosed in mesh or not. Moisture: the soil moisture of the collected core in grams water per grams dry soil. weighted copy_number: the bacterial community weighted rrn operon copy number. AP: activity rate of acid-phosphatase in micromoles per gram soil  per hour. BG: the activity rate of beta-glucosiadase in micromoles per gram soil per hour. Also included are files of the code used to analyze the data in the R Statistical Computing Environment.

opencc-by-4.0Apr 2024View details →

Georgetown Outbreak Activity Library (GOAL) dataset

The Georgetown Outbreak Activity Library (GOAL) dataset is a research effort led by Dr. Rebecca Katz at the Georgetown University Center for Global Health Science and Security. The data is designed to support responders in understanding what needs to get done, by whom, and when in the context of an event, providing information on existing guidance and authorities and to define response requirements for new and emerging events. GOAL can also be used for preparedness and planning efforts to ensure that this work is comprehensive and based in the practical realities of outbreak response. The GOAL dataset is available as an Excel file (.xlsx) which includes data coded about response activities needed throughout all phases of an outbreak, with case studies available to exemplify these activities during preparedness, response, and recovery efforts. The GOAL dataset contains a comprehensive set of fields describing what needs to be done for each response activity, when, by whom, and under what circumstances. A Data Dictionary and Glossary are included in the Excel file download. In addition to the coded data, the GOAL Case Study PDF Files (.zip) folder contains the original case studies written by Georgetown researchers which exemplify many of the activities described in the GOAL dataset.  To learn more about the project and interact with the data, visit <a href="https://outbreaklibrary.org/">https://outbreaklibrary.org/.</a> The complex data contained within the GOAL dataset have also been translated for a public audience into The Outbreak Atlas, a book by Rebecca Katz and Mackenzie S. Moore.

opencc-by-4.0Apr 2024View details →

Yoga Asana Increases Pre-Frontal Cortex Activity and Reduces Resting State Functional Connectivity

This dataset characterizes changes in the prefrontal cortex (PFC) before, during and after Yoga Asana (physical postures) with the mobile neuroimaging technique of functional near-infrared spectroscopy (fNIRS). Measurements were conducted with twenty-seven healthy adults executing four basic Asanas for 23 minutes with each Asana maintained for 25 -30 seconds. All postures significantly increased PFC activity versus baseline and resting state functional connectivity showed a significant decrease post Yoga Asana. Files 8, 15 and 24 were removed due to poor signal quality. During the measurement process of Asana the following stim marks were used to distinguish between postures: Posture A (Tadasana): A Posture B (Uttanasana): B Posture C (Adho Mukah Svasana): C Posture D (Urdhva Muka Svasana): D Results of the repeated measures ANOVA are presented for each combination of Asana. Those showing a significant difference are highlighted in green in the second to last tab of the file (Final Table). Demographics of volunteers are outlined in the last tab of the excel file (Demographics Volunteers).

Fig. 7. a in Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice

Fig. 7. a) A section of the small intestine in GV revealed normal villous architecture with a normal brush border (H&E stain, X200); b) Sections examined from the liver in this group showed preserved hepatic lobular architecture (H&E stain, X200).

Fig. 6. a in Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice

Fig. 6. a) A section of the small intestine in GIV revealed marked villous broadening (red line) with decreased villous height to crypt length ratio. There was dense infiltration by mononuclear inflammatory cells within the villous core (green arrows), degeneration of the villous tip-regions (black arrows), and increased mucin production (H&E stain, X200). b) Sections from the small intestine revealed many adherents (red arrows) and separate (black arrows) Cryptosporium stages, probably oocyst (H&E stain, X1000). c)Sections examined from the liver in this group showed hepatocellular degeneration (black arrow) and focal mononuclear cellular infiltration (red arrows) (H&E stain, X200)

Fig. 5. a in Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice

Fig. 5. a) A section of the small intestine in GIII revealed moderate villous broadening, infiltration by mononuclear inflammatory cells within the villous core (red arrow), focal degeneration of the villous tip regions (black arrow), and increased mucin production (H&E stain, X200). b) Sections examined from the liver in this group showed focal mononuclear cellular infiltration (red arrow) (H&E stain, X200).

Fig. 3. a in Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice

Fig. 3. a) A section of the small intestine in GI revealed villous broadening (red line) with an expansion of the villous core by mononuclear inflammatory cells (black arrow) (H&E stain, X200); b) Sections examined from the liver in this group showed preserved hepatic lobular architecture and cloudy swelling of hepatocytes (H&E stain, X200).

Fig. 1. a in Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice

Fig. 1. a) Cryptosporidium oocyst (stained red to deep purple with the modified Ziehl–Neelsen method); b) immunofluorescence staining of Cryptosporidium oocyst (ovoid or spherical brilliant apple/ green structure)

Fig. 2 in Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice

Fig. 2. Agarose gel electrophoresis showing: Lane 1: 50 bp DNA molecular weight marker, Lane 2: Positive control, Lane 3: Negative control, Lane 4: Positive sample of nested PCR products targeting COWP gene of Cryptosporidium at 553 bp, Lane 5: 50 bp DNA molecular weight marker, and Lane 6: RFLP products of the sample after digestion with RsaI endonuclease (C. parvum genotype 2 digestion products at 410, 106, and 34 (too small to be detected) bp.

Fig. 4. a in Anti-cryptosporidial activity of Camellia sinensis (green tea extract) in experimentally infected immunocompromised mice

Fig. 4. a) A section of the small intestine in GII revealed returning of the normal villous pattern, normal mucosa, and goblet cells (H&E stain, X200). b) Sections examined from the liver in this group showed preserved hepatic lobular architecture (H&E stain, X200).

opencc-by-4.0May 2024View details →

Effect of psychoactive substances on cardiac and locomotory activity-Procambarus virginalis-DATA

This Effect of psychoactive substances on cardiac and locomotory activity-Procambarus virginalis The associated dataset investigates the impact of environmentally relevant levels of psychoactive compounds on the early developmental stages of marbled crayfish (Procambarus virginalis). The compounds analyzed include sertraline, methamphetamine, and a mixture of citalopram, oxazepam, sertraline, tramadol, venlafaxine, and methamphetamine, each at a concentration of 1 μg L-1. The dataset assesses cardiac and locomotory activity on day four and day eight of exposure, respectively. it is a condition of the CENAKVA RI financing provider to record accesses to open data. Data can be requested at the following email: dubova@frov.jcu.cz or visit the CENAKVA RI website: https://www.frov.jcu.cz/en/faculty/faculty-parts/south-bohemian-research-centre-for-aquaculture-and-biodiversity-of-hydrocenoses-cenakva/large-research-infrastructure-cenakva

FIGURE 3 in Daily rhythm of locomotor and reproductive activity in the annual fish Garcialebias reicherti (Cyprinodontiformes: Rivulidae)

FIGURE 3 | Contextual modulation of locomotor activity of Garcialebias reicherti. Total daily locomotor activity for isolated (n = 10) and paired fish (n = 10). Each dot represents the mean number of events for each fish. *shows statistical significance (see p value in the main text). Box height from upper to lower quartile, whiskers represent standard deviation, median shown by horizontal line.

FIGURE 2 in Daily rhythm of locomotor and reproductive activity in the annual fish Garcialebias reicherti (Cyprinodontiformes: Rivulidae)

FIGURE 2 | Daily rhythm of locomotor activity in paired fish of Garcialebias reicherti recorded during four days in LD. A. Representative time series (actograms) and cosinor fit diagrams for two female (orange) and a male (green) dyads. Amount of locomotor activity (number of events) is normalized for visualization purposes. Gray areas in the actogram represent the dark phase of each 24 h period. The cosinor representation for each individual of the dyad is shown next to the actogram. Black outlines represent the duration of the night. The internal circumference depicts the p = 0.05 confidence limit. Radial lines show the extreme values of the acrophases calculated for each of six days. B. Rayleigh test for all paired individuals (5 males and 5 females) analyzed collectively. Triangles signal individual male acrophases, diamonds signal individual female acrophases. Members of each dyad are presented in the same color. The internal circumference depicts the p = 0.05 confidence limit and length of the black radial line marks the p value (external circumference is p = 0, see main text for p value). The black outline represents the duration of the night.

FIGURE 4 in Daily rhythm of locomotor and reproductive activity in the annual fish Garcialebias reicherti (Cyprinodontiformes: Rivulidae)

FIGURE 4 | Number and allocation of reproductive events at different hours in Garcialebias reicherti. Bars show the occurrence of events at different timepoints for each dyad (see references). Concentric circumferences show the number of events at that hour throughout the 4-day period. Black outline signals the duration of the night.

FIGURE 1 in Daily rhythm of locomotor and reproductive activity in the annual fish Garcialebias reicherti (Cyprinodontiformes: Rivulidae)

FIGURE 1 | Daily rhythm of locomotor activity in isolated fish of Garcialebias reicherti recorded during eight days in LD. A. Representative actograms and cosinor fit diagrams for a female (orange) and a male (green) fish. Amount of locomotor activity (number of events) is normalized for visualization purposes. Gray areas in the actogram represent the dark phase of each 24 h period. The cosinor representation for each individual is shown below the actogram. Black outlines represent the duration of the night. The internal circumference depicts the p = 0.05 confidence limit. Radial lines show the extreme values of the acrophases calculated for each of six days. B. Rayleigh test for all isolated individuals (5 males and 5 females) analyzed collectively. Triangles signal individual acrophases. The internal circumference depicts the p = 0.05 confidence limit and length of the black radial line marks the p value (external circumference is p = 0, see main text for p value). The black outline represents the duration of the night.

opencc-zeroMay 2024View details →

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Data from: Grazing herbivores reduce herbaceous biomass and fire activity across African savannas

Fire and herbivory interact to alter ecosystems and carbon cycling. In savannas, herbivores can reduce fire activity by removing grass biomass, but the size of these effects and what regulates them remain uncertain. To examine grazing effects on fuels and fire regimes across African savannas, we combined data from herbivore exclosure experiments with remotely sensed data on fire activity and herbivore density. We show that, broadly across African savannas, grazing herbivores substantially reduce both herbaceous biomass and fire activity. The size of these effects was strongly associated with grazing herbivore densities and, surprisingly, was mostly consistent across different environments. A one-zebra increase in herbivore biomass density (~100 kg/km2 of metabolic biomass) resulted in a ~53 kg/ha reduction in standing herbaceous biomass and a ~0.43 percentage point reduction in burned area. Our results indicate that fire models can be improved by incorporating grazing effects on grass biomass.

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Data from: Dissecting gene activation and chromatin remodeling dynamics in single human cells undergoing reprogramming

During cell fate transitions, cells remodel their transcriptome, chromatin, and epigenome; however, it has been difficult to determine the temporal dynamics and cause-effect relationship between these changes at the single-cell level. Here, we employ the heterokaryon-mediated reprogramming system as a single-cell model to dissect key temporal events during early stages of pluripotency conversion using super-resolution imaging. We reveal that, following heterokaryon formation, the somatic nucleus undergoes global chromatin decompaction and removal of repressive histone modifications H3K9me3 and H3K27me3 without acquisition of active modifications H3K4me3 and H3K9ac. The pluripotency gene OCT4 (POU5F1) shows nascent and mature RNA transcription within the first 24 h after cell fusion without requiring an initial open chromatin configuration at its locus. NANOG, conversely, has significant nascent RNA transcription only at 48 h after cell fusion but, strikingly, exhibits genomic reopening early on. These findings suggest that the temporal relationship between chromatin compaction and gene activation during cellular reprogramming is gene context dependent.

opencc-zeroMay 2024View details →

opencc-by-nc-4.0May 2024View details →

Metadata of the study HMGCR activity is essential for mitochondrial β-oxidation of fatty acids to prevent lethal accumulation of long-chain acylcarnitines in the mouse liver

Metadata of the study "HMGCR activity is essential for mitochondrial β-oxidation of fatty acids to prevent lethal accumulation of long-chain acylcarnitines in the mouse liver" published in Br J Pharmacol 2024. Apr 19. doi: 10.1111/bph.16363.

opencc-by-4.0May 2024View details →

Data for "The Role of Mood and Stress in Physical Activity Engagement: Insights from Ecological Momentary Assessment

Data collected in the SmartPA study in a joint effort from the Department of Psychology at the University of Salzburg (Jens Blechert) and the Ludwig Boltzmann Institute for Digital Health and Prevention Salzburg.