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621 results for “methodologies”
ISRH-seq: a sensitive and robust methodology for R-loop profiling
GEO Series GSE232347. Oryza sativa. 18 samples. Type: Other.
The development of a high-plex spatial proteomic methodology for the characterisation of the head and neck tumour microenvironment
GEO Series GSE288406. Homo sapiens. 136 samples. Type: Other.
Optimization of NaIO4 Oxidation and Deep-sequencing Methodology for Detection of 3' End Methylation of Small RNAs in low-input RNA Samples
GEO Series GSE130106. Mus musculus. 12 samples. Type: Non-coding RNA profiling by high throughput sequencing.
The ABRF Next-Generation Sequencing Study (ABRF-NGS): Multi-platform and cross-methodological reproducibility of transcriptome profiling by RNA-seq [454 GS FLX Titanium]
GEO Series GSE48032. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
The ABRF Next-Generation Sequencing Study (ABRF-NGS): Multi-platform and cross-methodological reproducibility of transcriptome profiling by RNA-seq [Ion Torrent Proton]
GEO Series GSE48034. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
Major impact of sampling methodology on gene expression in estrogen receptor positive breast cancer
GEO Series GSE105777. Homo sapiens. 426 samples. Type: Expression profiling by array.
The ABRF Next-Generation Sequencing Study (ABRF-NGS): Multi-platform and cross-methodological reproducibility of transcriptome profiling by RNA-seq [Ion Torrent PGM]
GEO Series GSE48033. Homo sapiens; synthetic construct. 21 samples. Type: Expression profiling by high throughput sequencing.
Methodology for objective assessment of Bradykinesia in Parkinson's disease
<p>Parkinson's disease (PD) is a potentially, devastating and multifactorial neurodegenerative disorder, linked to genetic and environmental factors, which compromises motor and non-motor functions. One difficulty in evaluating the PD progress is related to the prolonged time for clinical assessments and questionnaires application, the complexity and heterogeneity of motor impairments and the need for a specialized analysis. Bradykinesia is a Parkinson's disease symptoms, characterized as the slowness in the execution of movements, such as the reaction time to start the movements, the increase the range of movement during the execution of the tasks time for the duration of the motor pattern, the rapid fatigue in performing prolonged tasks and changing rhythm during activities. The assessment of bradykinesia severity can be influenced by the examiner's time of experience, by the patient's cooperation, as by the variability of inter-examiner results. The Movement Disorder Society (MDS) highlighted the need to develop and validate technologies for remote assessment of motor condition in PD. In this sense, this research presents two distinct strategies for the bradykinesia assessment, which are: (i) intra and inter-rater remote assessment of bradykinesia in Parkinson's disease and (ii) objective assessment, using inertial sensors, with the aim of discriminating the bradykinesia components: slowness, amplitude and rhythm. To the first objective (i), a mediation team performed and recorded the clinical evaluation MDS-UPDRS (Movement Disorder Society – Unified Parkinson's Disease Rating Scale) and edited all the videos, to later available the videos to 14 evaluators with varying experience, between 3 and 10 years, in Parkinson's disease evaluation. For intra and inter-rater analysis, statistical methods were applied between evaluations, Cohen's Kappa, intraclass coefficient correlation (ICC) and the Bland-Altman method. The correlation results obtained between experience raters are strong. Difficulty in clinical assessments can be influenced by lack of training and difficulty using clinical scales. Correlation matches also revealed low agreement due inexperience and poorly trained examiners. Despite flaws in clinical assessments, a large number of clinical raters, using blinded methods and gold standard rating scales, provide a more robust "fundamental truth" of bradykinesia assessment. It is important to highlight that the entire study execution was only possible due the mediator group compliance with the protocol. The effectiveness and feasibility of telemedicine during the COVID-19 pandemic is considered a breakthrough in medical care, enabling remote access, even outside the pandemic context. In the second objective of thesis (ii), the aim was to evaluate the bradykinesia components: slowness, amplitude and rhythm, extracted from inertial signals. The experimental protocol consisted of four tasks perform: finger tapping, open and close the hand, pronation and supination and flexion and extension. For that, inertial sensors were positioned at the upper limb for movements analysis. Fifteen individuals with Parkinson's disease and fifteen healthy individuals with matched sex and age performed this protocol. Signal processing was performed in R software and three feature extraction methods were used. The algorithm SFA (Slow Feature Analysis) was applied to extract the slowness characteristic. An index was created to extract the amplitude, based on the total amplitude and interquartile amplitude, and the rhythm was quantified by the variation coefficient. The component indices were correlated to the clinical assessment and statistical metrics for inter-group comparison. The obtained results indicate strong correlations between the bradykinesia components and clinical assessments, between -0.9 and -0.8 for amplitude and slowness, respectively. And for intergroup comparisons, the three indices showed a statistically significant difference. With this study, it can be concluded that the processing tools chosen to extract the characteristics of the inertial sensors were adequate, innovated and efficient in the discrimination of the components of bradykinesia.</p>
Innovative Methodologies Based on eXtended Reality and Immersive Digital Environments in Natural Risk education: A Scoping Review
<p><span>Faced with the rise in natural disasters, studies on Disaster Risk Reduction Education (DRRE) have emerged since the ‘90s, predominantly employing a transmissive teaching approach, while literature advocates for interactive models, including eXtended Reality (XR) simulations, which offer cost-effective solutions. This scoping review explores XR in DRRE for teachers, students, and citizens aiming to discern its pedagogical affordances. The databases search identified 34 papers published between 2013 and 2023. The majority centered on seismic events and floods, with Asia, notably Japan, as a primary source. Methodologically, 26 were empirical, using various research designs, and 8 were non-empirical. While XR-based tools demonstrated pedagogical affordances in teaching risk management, the lack of specific educational frameworks and a predominant focus on the acquisition of procedural knowledge and skills indicate that a broader approach is needed, by the incorporation of uncertainty education and complex competences, including attitudes like risk perception.</span></p>
'Dzīve līdzās ostai' aptauja: metodoloģija un neapstrādāti dati ('Living Next to the Port' survey: methodology and raw data)
<p>[English - below]</p> <p>Projekta atsauce:<br> «Dzīve līdzās ostai: ekonaratīvi, vietējā vēsture un vides aktīvisms Daugavas lejtecē» (Nr. lzp-2018/1-0446). Projekta vadītāja Dr. philol. Dace Bula.<br> Daļa datu analizēti grāmatā:<br> Dace Bula (zin. red.). Dzīve līdzās ostai. Rīga: LU LFMI, 2022. 184. lpp.</p> <p>Te deponēts:<br> -- aptaujas metodoloģija (latviski, angliski)<br> -- aptaujas anketa (latviski, krieviski, ar tulkojumu angliski)<br> -- aptaujas rīka LimeSurvey faili (kods)<br> -- aptaujas datu kopsavilkuma, krustojuma tabulas no neanonimizētiem datiem, kā nesvarotiem, tā svarotiem (*.xlsx; *.doc; *.pdf)<br> -- vides satraukuma indeksa tabula (*.xlsx; *.csv; *.doc; *.pdf [atsevišķi publicēta arī https://doi.org/10.5281/zenodo.7305008 un <br> https://docs.google.com/spreadsheets/d/1k4o1cuNXLolHwqVKM1m2tR-5Xgb0Uk0T9OyusYUtUyo/edit?usp=sharing])<br> -- neanonimizēti aptaujas dati (*.sav; *.xlsx [ar kodiem]; *.xlsx [ar vērtībām]; *.csv)<br> -- aptaujas datu faila kodu grāmata (codebook) (*.xlsx; *.txt)<br> -- SPSS sintakses (*.sps)<br> -- projekta vizuālā identitāte (autore -- Marika Latsone)<br> -- projekta un aptaujas reklāmattēli</p> <p>Piezīmes.</p> <p>-- Tehniski veicām četras aptaujas (četru apkaimju), šis kods un anketas ir no Mangaļsalas iedzīvotāju aptaujas.<br> -- Datu failā ir visu četru aptauju dati kopā.<br> -- Aptaujas tika veiktas divās valodās - LV, RU (LimeSurvey kodā ir abu valodu anketas). Anketa tulkota arī angliski.<br> -- Aptauja veikta ar LimeSurvey V. 3.25.10+210128.<br> -- Anonimizēti dati atvērtā pieejā publicēti atsevišķi -- https://zenodo.org/deposit/6483181.</p> <p>----------------</p> <p>Project reference:<br> “Living Next to the Port: Eco-Narratives, Local Histories and Environmental Activism in the Daugava Delta” (lzp-2018/1-0446). Project leader Dr. philol. Dace Bula.<br> Part of the data analysed in the book:<br> Dace Bula (ed.). Dzīve līdzās ostai [Living Next to the Port]. Riga: LFMI, 2022. p. 184.</p> <p>Deposited here:<br> -- Survey methodology (in Latvian, English)<br> -- Questionnaire (in Latvian, Russian, with English translation)<br> -- LimeSurvey survey tool files (code)<br> -- Summary, intersection tables from non-anonymised data, both unweighted and weighted (*.xlsx; *.doc; *.pdf)<br> -- Environmental Concern Index table (*.xlsx; *.csv; *.doc; *.pdf [also published separately at https://doi.org/10.5281/zenodo.7305008 and <br> https://docs.google.com/spreadsheets/d/1k4o1cuNXLolHwqVKM1m2tR-5Xgb0Uk0T9OyusYUtUyo/edit?usp=sharing])<br> -- Raw data (*.sav; *.xlsx [with codes]; *.xlsx [with values]; *.csv)<br> -- Codebook of the survey data file (*.xlsx; *.txt)<br> -- SPSS syntax (*. sps)<br> -- Visual identity of the project (author -- Marika Latsone)<br> -- Project and survey promotional images</p> <p>Notes.</p> <p>-- Technically we conducted four surveys (four neighbourhoods), this code and questionnaires are from the survey of residents of Mangalsala.<br> -- The data file contains data from all four surveys together.<br> -- The surveys were conducted in two languages -- Latvian and Russian (the LimeSurvey code contains questionnaires in both languages). The questionnaire is also translated into English.<br> -- The survey was conducted with LimeSurvey V. 3.25.10+210128.<br> -- Anonymised survey data in open access published separately -- https://zenodo.org/deposit/6483181.</p> <p> </p> <p>LU Literatūras, folkloras un mākslas institūts<br> Mūkusalas iela 3<br> Rīga, LV-1423<br> Tālr. (371) 67229017<br> info@lulfmi.lv<br> http://lulfmi.lv</p> <p>https://www.facebook.com/DziveLidzasOstai<br> http://lulfmi.lv/Dzive-lidzas-ostai<br> https://zenodo.org/communities/ekohum/<br> http://garamantas.lv/lv/repository/1537251/LZP-projekts-Dzive-lidzas-ostai<br> http://lulfmi.lv/en/Living-Next-to-the-Port [EN]</p> <p>Atsauce/ Reference<br> https://zenodo.org/record/7348880<br> https://doi.org/10.5281/zenodo.7348880</p>
Fig. 3 in A comprehensive review: Biological activity, modification and synthetic methodologies of prenylated flavonoids
Fig. 3. (continued).
Fig. 3 in A comprehensive review: Biological activity, modification and synthetic methodologies of prenylated flavonoids
Fig. 3. Chemical structures of prenylated flavonoids with cytotoxicity (1–196).
Genetic stabilization in Saccharomyces intra- and interspecific hybrids obtained by different hybridization methodologies
GEO Series GSE46192. Saccharomyces cerevisiae. 1 samples. Type: Genome variation profiling by array.
Statistical methodology for the evaluation of leukocyte data in wild reptile populations: a case study with the Common wall lizard (Podarcis muralis)
<p>The leukocyte profile has the potential to be a reliable method to measure health conditions and stress in wild animals, but limitations occur because current knowledge on reference intervals is largely incomplete, especially because data come from studies on captive animals involving few individuals from single populations. Here we propose a general framework for achieving reliable leukocyte reference intervals, encompassing a set of internal and external factors, potentially affecting the leukogram. To do so, we present a systematic survey of the hematology of the common wall lizard, <em>Podarcis muralis</em>, involving 794 lizards from 54 populations over the whole geographic range of the species in Italy. Reference intervals for white blood cell (WBC) and leukocyte differential count were obtained by using linear mixed models in a Bayesian framework. The application of the procedure clearly showed that both internal (sex and size) and external (latitude and season) factors are a source of variation of leukocyte profile. Furthermore, the leukogram of common wall lizard has a strong variability among populations, which accounts for more than 50% of the whole variation. Consequently, some common assumptions used in studies on captive individuals are no longer supported in wild populations, namely, i) any group of individuals is a representative sample, ii) any population is representative of all others, iii) geographic clines do not occur over the species range, and iv) seasonal variation has limited effects. We encourage researchers aimed at the definition of leukocyte reference intervals for wild populations of reptiles to involve a large number of populations over a wide geographic range in <em>ad hoc</em> statistical models to disentangle local and geographic effects on leukocyte profile variation.</p>
A methodological approach to identify priority zones for monitoring and assessment of wild bee species under climate change.
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Co-design multi-objective 3D optimization methodology for battery modules: An active air-cooling study case
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Tracing Data for Unveiling the Energy Vampires: A Methodology for Debugging Software Energy Consumption
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A methodological approach to identify priority zones for monitoring and assessment of wild species under climate change
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A new methodology using borehole data to measure angular distances between geological interfaces - Input and processed data
<p>This companion dataset relates to the manuscript "<strong>A new methodology using borehole data to measure angular distances between geological interfaces</strong>", by</p> <p>Michał P. Michalak<sup>a,b,</sup><a href="#sdfootnote1sym"><sup>1</sup></a>, Paweł Marzec<sup>b,</sup><a href="#sdfootnote2sym"><sup>2</sup></a>, Filip Turoboś<sup>c,</sup><a href="#sdfootnote3sym"><sup>3</sup></a>, Paulina Leonowicz<sup>d,</sup><a href="#sdfootnote4sym"><sup>4</sup></a>, Lesław Teper<sup>a,</sup><a href="#sdfootnote5sym"><sup>5</sup></a>, Paweł Gładki<sup>e,</sup><a href="#sdfootnote6sym"><sup>6</sup></a>, Michael J. Pyrcz<sup>f</sup><sup>,</sup><a href="#sdfootnote7sym"><sup>7</sup></a>,</p> <p>Mariusz Szubert<sup>g,</sup><a href="#sdfootnote8sym"><sup>8</sup></a></p> <p><a href="#sdfootnote1anc">1</a> Michał Michalak devised the project, wrote the manuscript, performed the computations and discussed the results.</p> <p><a href="#sdfootnote2anc">2</a> Paweł Marzec conducted the geological interpretation and discussed the results.</p> <p><a href="#sdfootnote3anc">3</a> Filip Turoboś conducted the statistical analysis.</p> <p><a href="#sdfootnote4anc">4</a> Paulina Leonowicz prepared the chapter about stratigraphy.</p> <p><a href="#sdfootnote5anc">5</a> Lesław Teper prepared the chapter about regional geology.</p> <p><a href="#sdfootnote6anc">6</a> Paweł Gładki participated in the study conceptualisation (discussion about distance functions)</p> <p><a href="#sdfootnote7anc">7</a> Michael Pyrcz discussed the applications of the method and revised the statistical section.</p> <p><a href="#sdfootnote8anc">8</a> Mariusz Szubert was responsible for the data acquisition.</p> <p>The archive contains the input and processed data. The input data contains XYZ coordinates of points documenting the investigated interfaces. The output files contains calculated orientations and coordinates of vectors. The output files can be processed in RStudio.</p>
Knowledge of dental postgraduate students on evidence-based dentistry and research methodology. An international survey
<p>Dataset for the analyses of the paper</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.