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opencc-by-4.0Nov 2019View details →

High resolution ICON simulation over the Tropical Atlantic

<p>This dataset contains binary info on liquid water content of an ICON simulation performed over the subtropics. Detailed information available in the readme file. This data is used in the publication titled: On the size dependence of cumulus cloud spacing.</p> <p> </p>

Figs 24–27 in On the Nature of Tintinnid Loricae (Ciliophora: Spirotricha: Tintinnina): a Histochemical, Enzymatic, EDX, and High-resolution TEM Study

Figs 24–27. Transmission electron micrographs of a lorica surface in Eutintinnus angustatus at middle (24) and higher (25–27) resolution. 24 – crystal lattice; 25 – digitally enlarged detail of Fig. 24 (bottom part). The periodicity of the hexagonal structures amounts to ~ 23.7 nm with a resolution of ~ 3 nm for the smallest details; 26 – fast Fourier transform of Fig. 25; 27 – noise filtered bright field image after inverse Fourier transform of Fig. 26, using only the diffraction spots. Due to the enhanced contrast, the ultrastructure of the crystal lattice appears more distinct.

Figs 20–23 in On the Nature of Tintinnid Loricae (Ciliophora: Spirotricha: Tintinnina): a Histochemical, Enzymatic, EDX, and High-resolution TEM Study

Figs 20–23. Transmission electron micrographs of a lorica surface in Eutintinnus angustatus at middle resolution. 20 – a crystalline region ~ 1.5 µm in diameter is shown in the centre of the micrograph. An aggregate of bitter salt (MgSO 4) is attached to the wall (arrow); 21 – crystalline area at higher magnification showing dark spots of sodium and potassium chloride on the lorica wall; 22 – each black spot represents a NaCl or KCl nanocrystal (arrows), which has almost the same size as the unit cells of the crystal lattice (~ 20 nm); 23 – Fourier filtered high-resolution micrograph of a sodium chloride nanocrystal.

Fig. 15. Energy-dispersive X in On the Nature of Tintinnid Loricae (Ciliophora: Spirotricha: Tintinnina): a Histochemical, Enzymatic, EDX, and High-resolution TEM Study

Fig. 15. Energy-dispersive X-ray spectrometric (EDX) analysis in the scanning electron microscope, using uncoated material. The analysed area of the Eutintinnus angustatus lorica is marked by a white frame (~ 11 × 9 µm in size). Since this part of the lorica was freely suspended in the vacuum, elemental detection occurred without influence of the carbon substrate.

Figs 16–19 in On the Nature of Tintinnid Loricae (Ciliophora: Spirotricha: Tintinnina): a Histochemical, Enzymatic, EDX, and High-resolution TEM Study

Figs 16–19. Transmission electron micrographs of an uncoated lorica surface of Eutintinnus angustatus at different magnifications. 16 – overview of right lorica half. The lorica lies nearly horizontally on the electron transparent holey carbon substrate. The black rectangular structures are the copper bars of the TEM grid; 17 – anterior portion of right lorica half; 18 – apical lorica portion; 19 – lateral lorica portion. The dark crystalline dendritic structures consist of sodium chloride nanocrystals, which probably originate in the sea water.

opencc-by-4.0Aug 2019View details →

Fig. 1 in Species-specific qPCR assays allow for high-resolution population assessment of four species avian schistosome that cause swimmer's itch in recreational lakes

Fig. 1. Abundance of cercariae by sampling site. Water samples were obtained in mid-June and cercariae abundance was determined using the pan-avian schistosomes qPCR.

opencc-by-4.0Aug 2019View details →

Fig. 2 in Species-specific qPCR assays allow for high-resolution population assessment of four species avian schistosome that cause swimmer's itch in recreational lakes

Fig. 2. Percent contribution of T. stagnicolae, T. szidati, T. physellae and A. brantae species to each lake. Water samples from different locations and dates were tested using the species-specific qPCR assay and results were pooled by lake to understand the relative contribution overall of each species to each lake. The percent contribution (based on gene copy number) of each species was calculated.

opencc-by-4.0Aug 2019View details →

Fig. 3 in Species-specific qPCR assays allow for high-resolution population assessment of four species avian schistosome that cause swimmer's itch in recreational lakes

Fig. 3. Lifecycles of T. stagnicolae, A. brantae, T. szidati, and T. physellae. life cycle summary of the avian schistosome species targeted for species-specific qPCR tests designed in this study.

Fig. 3 in Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR)

Fig. 3. Phylogenetic tree of seven Trypanosome species and subsequent genotypes constructed with sequences of the amplicons generated by the HRMqPCR primers.

Fig. 4 in Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR)

Fig. 4. Amplification plots, melt curves and standard curves of T. copemani, T. vegrandis G7 and T. noyesi G8 prepared from a plasmid containing trypanosome species.

Fig. 5. A-D in Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR)

Fig. 5. A-D: Derivative melt curves showing mock mixed infections generated from plasmid clones containing the following DNA: (A) T. noyesi G8 and T. copemani; (B) T. vegrandis G7 and T. copemani; (C) T. vegrandis G7 and T. noyesi G8; (D) T. vegrandis G7, T. noyesi G8 and T. copemani.

Fig. 1 in Species-level identification of trypanosomes infecting Australian wildlife by High-Resolution Melting - Real Time Quantitative Polymerase Chain Reaction (HRM-qPCR)

Fig. 1. Multiple sequence alignment of a portion of the 18S rDNA of seven Trypanosome species and subsequent genotypes used to design the HRM-qPCR assays.

Fig. 4 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 4. Metacercariae of Diplostomum spp. from eye lenses of different fish hosts; (a) Diplostomum sp. from Tilipia sparrmanii, live, ventral view (b) Diplostomum sp. from Tilipia sparrmanii, fixed, ventral view (c) Diplostomum sp. from Tilipia sparrmanii, live, sunken pseudosuckers (arrowhead) (hologenophore, GenBank MN813526, MN813534 and MN808616) (d) Diplostomum sp. 14 sensu Locke et al. (2015) from Synodontis zambezensis, live, ventral view (hologenophore, GenBank MN813541) (e) Diplostomum sp. 14 sensu Locke et al. (2015) from Oreochromis mossambicus, fixed, ventral view, small excretory granules (arrowhead) (hologenophore, GenBank MN813531, MN813539 and MN808621) (f) Diplostomum sp. 14 sensu Locke et al. (2015) from Synodontis zambezensis, fixed, ventral view, large excretory granules (arrowhead) (hologenophore, GenBank MN813541) (g) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, fixed, ventral view, everted pseudosuckers (arrowhead) (hologenophore, GenBank MN813532, MN813547 and MN808627) (h) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, fixed, ventral view, inverted pseudosuckers (arrowhead) (hologenophore, GenBank MN813533, MN813548 and MN808628) (i) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, live metacercariae inside of fish lens. Scale bars: a–h = 100 μm; i = 700 μm.

Fig. 3 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 3. Bayesian inference (BI) and maximum likelihood (ML) phylogram reconstructed using cox1 sequences for species of Diplostomum. Nodal support from BI and ML analyses indicated as BI/ML; only values> 0.90 (BI) and> 70 (ML) are displayed. Scale-bar indicates the expected number of substitution per site. Sequences generated in this study are in bold and indicated by blue rectangles. Codes with isolate information for newly generated sequences are provided in Table 3. Sequences derived from Africa are highlighted in blue, from Asia in purple, from Europe in orange, from North America in green (according to the map) and sequences reported from more than one continent are highlighted in black. Black arrows on the map demonstrate distribution of Diplostomum spathaceum and 'D. mergi Lineage 2' in both, Asia and Europe, and Diplostomum sp. 14 and Diplostomum sp. 16 in both, Africa and Asia. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 2. Bayesian inference (BI) and maximum likelihood (ML) phylograms reconstructed using (a) partial 28S rDNA sequences (b) ITS1-5.8S-ITS2 sequences for species of Diplostomum. Nodal support from BI and ML analyses indicated as BI/ML; only values> 0.90 (BI) and> 70 (ML) are displayed. Scale-bar indicates the expected number of substitution per site. Sequences generated in this study are in bold and indicated by blue rectangles. Codes with isolate information for newly generated sequences are provided in Table 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 1. Map illustrating the sampling localities on (a) River Riet in Mokala National Park (b) River Phongolo (Site 1, Site 2 and Nyamithi Lake) and the River Usuthu (Shokwe Pan) in Ndumo Game Reserve and (c) River Mooi (Boskop Dam) in Boskop Dam Nature Reserve, South Africa. The illustration was compiled in ArcGIS 10.6 (Available from https://support.esri.com/en/downloads).

opencc-by-4.0Dec 2015View details →

Figure 2 in High-Resolution Functional Imaging of Native Proteins using Force Distance Curve Based Atomic Force Microscopy

Figure 2. - Juvenile Trachipterus arcticus, 129 mm SL, collected at Faial Island, Azores, 14 May 2014, on the surface. A: Portrait with anterior black facet visible; B: Oblique lateral view with first spines erected; note orange bulbous outgrowths on the prolonged spine; C: Lateral view showing proportions, markings and orientation of fins. Scale bars: A = 1 cm; B, C = 5 cm.

opencc-by-4.0Dec 2015View details →

Figure 1 in High-Resolution Functional Imaging of Native Proteins using Force Distance Curve Based Atomic Force Microscopy

Figure 1. - Adult Trachipterus arcticus, about 1.8 m long, observed south of Pico Island, Azores, 18 Aug. 2013, 950 m deep.

opencc-by-4.0Dec 2008View details →

Fig. 1 in A novel supermatrix approach improves resolution of phylogenetic relationships in a comprehensive sample of danthonioid grasses

Fig. 1. Geologic map of Florissant Fossil Beds National Monument in central Colorado, USA, modified from Evanoff et al. (2001: fig. 1). Areal extent of the Monument is outlined by thick gray line.