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738 results for “Microsatellite”
Data from: Microsatellite evidence for high frequency of multiple paternity in the marine gastropod Rapana venosa
Background: Inferring of parentage in natural populations is important in understanding the mating systems of a species, which have great effects on its genetic structure and evolution. Muricidae, a large group (approximately 1,600 species) of marine gastropods, are poorly investigated in patterns of multiple paternity and sperm competition based on molecular techniques. The veined Rapa whelk, Rapana venosa, a commercially important muricid species with internal fertilization, is an ideal species to study the occurrence and frequency of multiple paternity and to facilitate understanding of their reproductive strategies. Methodology/Principal Findings: We developed five highly polymorphic microsatellites in R. venosa and applied them to identify multiple paternity in 19 broods (1381 embryos) collected from Dandong, China. Multiple paternity was detected in 17 (89.5%) of 19 broods. The number of sires per brood ranged from 1 to 7 (4.3 on average). Of the 17 multiply sired broods, 16 (94.1%) were significantly skewed from equal paternal contributions, and had a dominant sire which was also dominant in each assayed capsule. Conclusions: Our results indicate that a high level of multiple paternity occurs in the wild population of R. venosa. Similar patterns of multiple paternity in the 2–6 assayed capsules from each brood imply that fertilization events within the body of a female occur mostly (but not entirely) as random draws from a "well-but-not-perfectly blended sperm pool" of her several mates. Strongly skewed distributions of fertilization success among sires also suggest that sperm competition and/or cryptic female choice might be important for post-copulatory paternity biasing in this species.
Data from: Evaluation of genetic diversity and population structure of five Chinese indigenous donkey breeds using microsatellite markers
China had the largest population of raising donkeys in the world, however the number of Chinese indigenous donkey decreased dramatically due to the increase of agriculture mechanization in the last century. The species has still been important in China because of its edible and medical value, therefore the survey on its genetic diversity in China is necessary for its conservation and utilization. In this study, 15 microsatellite markers were used to evaluate genetic diversity and population structure of five Chinese indigenous donkey breeds. The mean values of expected heterozygosity, allelic richness, and total number of alleles for all the tested Chinese donkeys were 0.70, 6.04, and 6.28 respectively, suggesting that the genetic diversity of Chinese indigenous donkeys is rich. The Bayesian analysis and principal component analysis plot yielded the same clustering result, which revealed that Guanzhong donkey was the most differentiated breed in all detected samples, and Jinnan (JN) and Guangling (GL) were genetically closed together. Additionally, our results indicated that the heterozygote deficit was severe in two Chinese indigenous donkey breeds (GL and JN), and it warned us that animal conservation activities on this species should be considered carefully in near future.
Fig. 1 in Microsatellite Variability Of Two Populations Of Clarias Gariepinus (Siluriformes, Clariidae) In Nigeria
Fig. 1. The study area showing the map of sites of collection of C. gariepinus.
T a b l e 7 in Molecular Characterization Of Lates Niloticus (Perciformes, Latidae) Populations From Three Nigerian Waterbodies Using Random Amplified Polymorphic Dna And Microsatellite Markers
T a b l e 7. Conformity to Hardy-Weinberg equilibrium
Microsatellites raw genotypes of Aedes koreicus mosquitoes
<p>Genotypes of single mosquito of the species <em>Aedes koreicus</em>; genotyping was performed using capillary electrophoresis (Fragment Length Analysis) by Eurofins Genomics at 10 loci specific for <em>Ae. koreicus</em>.</p>
Figure 1 from: Li Y, Lin L-S, Gao T-X (2018) Genetic signatures of polymorphic microsatellite loci in the Ambiguous silver pomfret, Pampus argenteus (Teleostei, Stromateidae). ZooKeys 810: 139-151. https://doi.org/10.3897/zookeys.805.25602
Figure 1 Locations (black circle) for sample collection of P.argenteus.
microsatellite data
<p>This is the microsatellite raw data of the article 'Revelation of genetic diversity and structure of wild <em>Elymus excelsus</em> (Poaceae: Triticeae) collection from western China by SSR markers' published at Peer J.</p>
Figure 1 from: Liu L, Zhang Y, Hu X, Lü Z, Liu B, Jiang LH, Gong L (2019) Multiple paternity assessed in the cuttlefish Sepiella japonica (Mollusca, Cephalopoda) using microsatellite markers. ZooKeys 880: 33-42. https://doi.org/10.3897/zookeys.880.33569
Figure 1 S. japonica mating in the head-to-head position.
Data from: Cost effective microsatellite isolation and genotyping by high throughput sequencing
High throughput sequencing (HTS) has emerged as a valuable tool for the rapid isolation of genetic markers for population genetics and pedigree analysis. HTS-based SNP (single nucleotide polymorphism) genotyping protocols like RAD (Restriction-site associated DNA) sequencing or hybrid capture, allow for the isolation of thousands of markers from any non-model organism. However, these protocols are relatively laborious and expensive and the resulting high marker density is not always necessary. Since HTS technology has also greatly simplified the isolation and genotyping process of microsatellite markers, we develop microsatellite markers as a cost efficient and simple alternative to SNP genotyping. We present low coverage genome sequencing data from seven distantly related spider species (Argiope bruennichi, Larinia jeskovi, Oedothorax restusus, Pisaura mirabilis, Australomisidia ergandros, Cheiracanthium punctorium, Theridion grallator) and show the utility of HTS for microsatellite isolation. We also present a simple Illumina amplicon sequencing protocol to genotype microsatellites from multiplex PCR amplicons in the Hawaiian happy face spider T. grallator. We discuss advantages and drawbacks of the use of microsatellites for a range of research questions, and highlight an unexpectedly fast decay and gain of repeat loci for T. grallator.
Three oaks Microsatellite data
<p>Knowledge of interspecific divergence and population expansions/contractions of dominant forest trees in response to geological events and climatic oscillations is of major importance to understand their evolution and demography. However, the interspecific patterns of genetic differentiation and spatiotemporal population dynamics of three deciduous <i><span>Cerris</span></i> oak species (<i>Q. acutissima</i>, <i>Q. variabilis</i> and <i>Q. chenii</i>) that are widely distributed in China remain poorly understood. In this study, we genotyped 16 nuclear loci in 759 individuals sampled from 44 natural populations of these three sibling species to evaluate the plausible demographical scenarios of the closely related species. We also tested the hypothesis that macro- and microevolutionary processes of the three species had been triggered and molded by Miocene–Pliocene geological events and Quaternary climatic change. The Bayesian cluster analysis showed that <i>Q. acutissima</i> and<i> Q. chenii </i>were clustered in the same group, whereas <i>Q. variabilis </i>formed a different genetic cluster. An approximate Bayesian computation (ABC) analyses suggested that <i>Q. variabilis </i>and<i> </i><i>Q. acutissima</i> diverged from their most common ancestor around 19.84 Ma, and subsequently <i>Q. chenii </i>diverged from <i>Q. acutissima </i>at about<i> </i>9.6 Ma, which was significantly associated with the episodes of the Qinghai–Tibetan Plateau (QTP). In addition, ecological niche modeling and population history analysis showed that these three <i><span>Cerris</span></i><i> </i>oak<i> </i>species repeatedly underwent considerable 'expansion–contraction' during the interglacial and glacial periods of the Pleistocene, although they have varying degrees of tolerance for the climatic change. Overall, these findings indicated geological and climatic changes during the Miocene–Pliocene and Pleistocene as causes of species divergence and range shifts of dominant tree species in the subtropical and warm temperature areas in China.</p>
Microsatellite data of 21 EST-SSR loci from all 469 Emmenopterys henryi samples
<p>Several hypotheses are available to predict change in genetic diversity at expanding range margins. However, empirical evidence to test predictions of the central-marginal hypothesis (CMH) at contracting range limits is scarce. To address this issue, we assessed spatial patterns of genetic variation, effective population size, and contemporary and historical gene flow in a widespread, Tertiary relict tree species from subtropical China, <i>Emmenopterys henryi</i>.</p>
Kimberley Fish microsatellite genotypes
<ol> <li>Dispersal is a critically important process that dictates population persistence, gene flow and evolutionary potential and is an essential element for identifying species conservation risks. This project aims to investigate the contributions of dispersal syndromes and hydrographic barriers on patterns of population connectivity and genetic structure in fishes occupying the particularly rugged and fragmented landscape of the Kimberley Plateau, Western Australia.</li> <li>We assessed population genetic structure between three neighbouring catchments (the Mitchell, King Edward and Drysdale rivers) in three congeneric groups of freshwater fishes that exhibit varied dispersal syndromes within and among groups: (1) <i>Melanotaenia australis</i> and <i>M. gracilis</i>; (2) <i>Syncomistes trigonicus </i>and <i>S. rastellus</i>; (3) <i>Hephaestus jenkinsi</i> and <i>H. epirrhinos</i>. Within each species we sampled the upper, middle and lower reaches of each catchment and assessed patterns of gene flow between and within catchments using microsatellite markers.</li> <li>Our results suggest that contemporary connectivity between catchments is greatly limited or absent in all study species, regardless of their dispersal syndromes. However, gene flow within catchments varied in line with predicted dispersal potential with poor dispersers exhibiting limited gene flow and significant genetic structuring.</li> <li>We conclude that the rugged landscape and historical habitat isolation has contributed to patterns of population fragmentation among fish populations from different river catchments. However, it appears dispersal syndromes influence connectivity and gene flow within catchments, where landscape constraints are not as pervasive.</li> <li>This study presents a comparative population genetic analysis of freshwater fishes with differing dispersal syndromes and colonisation ability. Our findings provide new insights into factors shaping patterns of biodiversity on the Kimberley Plateau, and the evolutionary uniqueness of fish communities from different river catchments draining the plateau. The results from this study provide a framework for informing the management of the region's unique freshwater biodiversity.</li> </ol>
A Trial of CXD101 in Combination With Nivolumab in Patients With Metastatic Microsatellite-Stable Colorectal Cancer
ClinicalTrials.gov study NCT03993626. IPD Sharing: NO. Countries: 1. Publications: 0.
Immunotherapies in Combination With Stereotactic Body Radiation Radiotherapy in Microsatellite Stable (MSS) Metastatic Colorectal Cancer (mCRC)
ClinicalTrials.gov study NCT06603818. IPD Sharing: YES. Countries: 1. Publications: 0.
Vitamin E Combined With Fruquintinib and Tislelizumab in Microsatellite Stabilized Metastatic Colorectal Cancer Patients
ClinicalTrials.gov study NCT05771181. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Efficacy and Safety of Combined With Immunotherapy After Induction Therapy With Chemotherapy and Targeted Therapy in the First-line Treatment of Microsatellite Stable (MSS) Initially Unresectable Meta
ClinicalTrials.gov study NCT06176885. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Phase Ib/II Study to Evaluate the Safety and Efficacy of Nivolumab in Combination With Paclitaxel in Epstein-Barr Virus(EBV)-Related, or Microsatellite Instability-High (MSI-H), or Programmed Cell Dea
ClinicalTrials.gov study NCT05535569. IPD Sharing: NO. Countries: 1. Publications: 0.
GAIA-102 Intraperitoneal Administration in Patients With Advanced Gastrointestinal Cancer of Microsatellite Stable With Malignant Ascites
ClinicalTrials.gov study NCT05438459. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Nous-209 Genetic Vaccine for the Treatment of Microsatellite Unstable Solid Tumors
ClinicalTrials.gov study NCT04041310. IPD Sharing: NO. Countries: 6. Publications: 0.
Botensilimab, Balstilimab and Regorafenib for the Treatment of Patients With Microsatellite Stable Metastatic Colorectal Cancer Who Have Progressed on Prior Chemotherapy
ClinicalTrials.gov study NCT05672316. IPD Sharing: Not stated. Countries: 1. Publications: 0.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.