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FIG. 2 in Morphological description and molecular phylogeny of two diatom clones from the genus Ulnaria (Kützing) Compère isolated from an ultraoligotrophic lake at the Pole of Cold in the Northern Hemisphere, Republic of Sakha (Yakutia), Russia
FIG. 2. — Ulnaria pilum Kulikovskiy & Lange-Bertalot LAB59: A, LM; B, SEM, external view and central area; C, internal view and central area; D, SEM, external view and the valve apex; E, internal view and the valve apex, rimoportula is located at an oblique angle relative to the central sternum; F, SEM, external view, two small spines at the apex. Scale bars: A, 50 µm; B-E, 5 µm; F, 1 µm.
Picea mariana isolate 40-10-1 mitochondrial genome assembly
<p><em>Picea mariana</em> isolate 40-10-1 mitochondrial genome assembly generated with Illumina HiSeq and 10x Genomics Chromium reads using ABySS v2.1.0, Tigmint v1.1.2, and ARCS v1.0.6.</p>
Picea glauca isolate WS77111 mitochondrial genome assembly
<p><em>Picea glauca</em> isolate WS77111 mitochondrial genome assembly generated with Illumina HiSeq reads using ABySS v2.1.4.</p>
Picea engelmannii isolate Se404-851 mitochondrial genome assembly
<p><em>Picea engelmannii</em> isolate Se404-851 mitochondrial genome assembly generated with Illumina HiSeq reads using ABySS v2.1.4.</p>
Evolution of reproductive isolation in a long-term evolution experiment with Drosophila melanogaster: 30 years of divergent life history selection
<p>We ask if three decades and over 1,500 generations of divergent life history selection on age at reproduction has resulted in the evolution of reproductive isolation (RI) between laboratory populations of <em>Drosophila</em> <em>melanogaster</em>. We tested for premating, postmating-prezygotic and postzygotic reproductive isolation between 3 replicate population pairs. Large evolved differences in body size between selection treatments suggested the potential for prezygotic barriers driven by sexual selection or physical incompatibilities between the sexes. Although a simple prediction would be preference for larger size, creating directional isolation, our results from individual mate choice trials indicate that populations from both selection treatments show a marked bias towards homotypic mate choice; indicative of prezygotic RI driven by sexual selection or sexual conflict. Hybridization between the focal populations resulted in the production of viable adult flies with intermediate size and developmental traits. We observed a suggestive but statistically non-significant trend of fitness decline in the F2 generation of hybrids, but no significant evidence suggesting the evolution of postmating-prezygotic or postzygotic RI. Our findings are in accord with extant literature that posits that premating RI evolves before postmating forms of RI.</p>
Area, isolation, and climate explain the diversity of mammals on island worldwide
<p>Identifying the determinants of insular biodiversity at large scales remains a question in biogeography. We conducted a global test of island biogeography theory by evaluating the importance of island physical, environmental, and historical characteristics on mammal species richness and endemism. We quantified the effects of island characteristics while accommodating variation among biogeographic realms by fitting generalized linear and mixed models. Analyzes were also performed separately for bats and non-volant mammals. Diversity patterns were most consistently influenced by the physical characteristics of the islands. Area positively affected mammal diversity, in particular the number of non-volant endemics. Island isolation, both current and past, was associated with lower richness but greater endemism. Flight capacity modified the relative importance of past versus current isolation, with bats responding more strongly to current and non-volant mammals to past isolation. Environmental effects on biodiversity were more variable, with a tendency for greater effects on endemism than on richness. Unexpectedly, climate change velocity was positively associated with endemism. In line with island biogeography theory, we found that area and isolation were among the strongest drivers of overall mammalian biodiversity. Moreover, our results support the growing evidence on the importance of past conditions on current patterns, particularly on non-volant species.</p>
Fig. 1 in Isolation and molecular characterization of Bacillus thuringiensis found in soils of the Cerrado region of Brazil, and their toxicity to Aedes aegypti larvae
Fig. 1. SDS-PAGE protein profiles of the Bacillus thuringiensis isolates most toxic to Aedes aegypti larvae. MM, molecular weight marker (kDa); Bti, Bacillus thuringiensis var. israelensis; 25–560, Bacillus thuringiensis isolates.
Figure 3 in Isolation of Bacillus thuricgiecsis from tce state of Amazonas, in Brazil, and screening against Aedes aegypti %Diptera, Culicidae)
Figure 3. Amplification products of gen cry11Ba of dipteran-specific genes isolated from Bacillus thuringiensis from the state of Amazonas, Brazil. Legend: MM: marker 1kb-sized DNA ladder; 03: IBt-03; 06: IBt-06; 07: IBt-07; 28: IBt-28; 30: IBt-30; 27: BtAM-27; 82: Bti IPS-82 (positive control); NC: negative control.
Figure 2 in Isolation of Bacillus thuricgiecsis from tce state of Amazonas, in Brazil, and screening against Aedes aegypti %Diptera, Culicidae)
Figure 2. Amplification products of gen (A) cry10Aa and (B) cry11A of dipteran-specific genes isolated from Bacillus thuringiensis from the state of Amazonas, Brazil.
Fig. 5. Folded 16S-23S in Newly recorded genera and species, Pantanalinema rosaneae and Alkalinema pantanalense (Leptolyngbyaceae, Cyanobacteria) isolated in Korea
Fig. 5. Folded 16S-23S rRNA ITS secondary structures of strains of (A) Pantanalinema rosaneae and (B) Alkalinema pantanalense.
Fig. 2 in Newly recorded genera and species, Pantanalinema rosaneae and Alkalinema pantanalense (Leptolyngbyaceae, Cyanobacteria) isolated in Korea
Fig. 2. Microphotographs of Pantanalinema rosaneae from the strain FBCC-A1471. (A-C) Colonies (mats) formed in culture, (D-F) Hormogonia, (G-I) Morphologies of apical cells, (J-L) Firm sheathes of trichomes in culture. Scale bars = (A) 100 μm, (B-L) 10 μm.
Fig. 1 in Newly recorded genera and species, Pantanalinema rosaneae and Alkalinema pantanalense (Leptolyngbyaceae, Cyanobacteria) isolated in Korea
Fig. 1. Photographs showing the collection sites of Pantanalinema rosaneae (A, B) and Alkalinema pantanalense (C, D). (A) The red circle is Chungjeong-ro 3-ga, Seodaemun-gu, Seoul (37°33ʹ51.1ʺN / 126°57ʹ38.6ʺE), (C) The red circle is Anseongcheon, Anseong-si, Gyeonggi-do (37°00ʹ04.2ʺN / 127°16ʹ13.5ʺE), and the habitat views of collection sites (red arrow) of (B) P. rosaneae and (D) A. pantanalense.
Fig. 3 in Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea
Fig. 3. The phylogenetic tree based on 16S rRNA gene sequence showing the phylogenetic relatedness between the four isolated strains belonging to the phylum Actinobacteria and the type species from each genus. The tree was mainly reconstructed using the NJ, ML, and MP algorithms. The filled diamonds indicate the branches present in the phylogenetic trees reconstructed with three different tree reconstruction methods. Numbers on the nodes represent bootstrap values with 1000 replicates (NJ/ML/MP). B. fragilis NCTC 9434T was used as an outgroup. The bar indicates 0.05 accumulated substitutions per nucleotide.
Fig. 2 in Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea
Fig. 2. The phylogenetic tree based on 16S rRNA gene sequence showing the phylogenetic relatedness between the nine isolated strains belonging to the phylum Proteobacteria and the type species from each genus. The tree was mainly reconstructed using the neighbor joining (NJ), maximum likelihood (ML), and maximum parsimony (MP) algorithms. The Filled diamonds indicate the branches present in the phylogenetic trees reconstructed using three different tree reconstruction methods. Numbers on the nodes represent bootstrap values with 1000 replicates (NJ/ ML/MP). Bacteroides fragilis NCTC 9434T was used as an outgroup. The bar indicates 0.05 accumulated substitutions per nucleotide.
Fig. 5 in Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea
Fig. 5. The phylogenetic tree based on 16S rRNA gene sequence showing the phylogenetic relatedness between the three isolated strains belonging to the phylum Firmicutes and the type species from each genus. The tree was mainly reconstructed using the NJ, ML, and MP algorithms. The filled diamonds indicate the branches present in the phylogenetic trees reconstructed using three different tree reconstruction methods. Numbers on the nodes represent bootstrap values with 1000 replicates(NJ/ML/MP). E. coli ATCC 11775T was used as an outgroup. The bar indicates 0.05 accumulated substitutions per nucleotide.
Fig. 1 in A report of unrecorded bacterial species of Korea isolated in 2016, belonging to the family Deinococcaceae and Planctomycetaceae
Fig. 1. Transmission electron micrographs of cells of the strains isolated in this study. The cells were cultured at their optimal growth conditions. Strains: 1, 6004; 2, CAU 1293.
Fig. 3 in A report of unrecorded bacterial species of Korea isolated in 2016, belonging to the family Deinococcaceae and Planctomycetaceae
Fig. 3. Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences shows the relationship between the strains isolated in this study and their relatives of the genus Blastopirellula. Bootstrap values (>70%) are shown above nodes for the neighbor-joining methods. Bar: 0.02 substitutions per nucleotide position.
Fig. 1 in Isolation and identification of 18 unrecorded prokaryotic species from the intestinal tracts of aquatic animals in Korea
Fig. 1. The energy-filtering transmission electron micrographs of the isolated strains. Strains: 1, K33R8; 2, K33T6; 3, G12R3; 4, R13S1; 5, R23R4; 6, R33M6-1; 7, R33M4; 8, K11M4; 9, O13M9; 10, 719; 11, 176; 12, 775; 13, 765; 14, 684; 15, A52; 16, 771; 17, A79; 18, 772.
Multiple mechanisms contribute to isolation-by-environment in the redheaded pine sawfly, Neodiprion lecontei
<p class="NormalThesis"><span>Isolation by environment (IBE) is a population genomic pattern that arises when ecological barriers reduce gene flow between populations. Although current evidence suggests IBE is common in nature, few studies have evaluated the underlying mechanisms that generate IBE patterns. In this study, we evaluate five proposed mechanisms of IBE (natural selection against immigrants, sexual selection against immigrants, selection against hybrids, biased dispersal, environment-based phenological differences) that may give rise to host-associated differentiation within a sympatric population of the redheaded pine sawfly, <em>Neodiprion lecontei</em>, a species for which IBE has previously been detected. We first characterize the three pine species used by <em>N. lecontei</em> at the site, finding morphological and chemical differences among the hosts that could generate divergent selection on sawfly host-use traits. Next, using morphometrics and ddRAD sequencing, we detect modest phenotypic and genetic differentiation among sawflies originating from different pines that is consistent with recent, <em>in situ</em> divergence. Finally, via a series of laboratory assays – including assessments of larval performance on different hosts, adult mate and host preferences, hybrid fitness, and adult eclosion timing – we find evidence that multiple mechanisms contribute to IBE in <em>N. lecontei</em>. Overall, our results suggest IBE can emerge quickly, possibly due to multiple mechanisms acting in concert to reduce migration between different environments.</span></p>
The daft genome sequences for the isolated viruses.
<p>The daft genome sequences for the viruses isolated from Lake Biwa, Japan. The genomes were sequenced by MinION and assembled by Flye, Miniasm, and Raven, followed by short-read polish by using Pilon. </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.