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opennotspecifiedSep 2014View details →

FIGURES 26–35 in Actinellopsis murphyi gen. et spec. nov.: A new small celled freshwater diatom (Bacillariophyta, Eunotiales) from Zambia

FIGURES 26–35. SEM images of external head and foot poles of Actinellopsis murphyi gen. et. spec. nov. Figs 26–30. SEM images of external view of head pole. Figs 31–35. SEM images of external view of foot pole. Rimoportula opening (R). Scale bar = 3 µm (26, 27), 2 µm (28, 29, 30, 31, 32, 33, 34, 35).

opennotspecifiedAug 2014View details →

FIGURE. 50 in The phylogenetic relationships of Torrendiella and Hymenotorrendiella gen. nov. within the Leotiomycetes

FIGURE. 50% majority-rule consensus tree based on a Bayesian analysis of SSU, 5.8S rRNA, and LSU gene sequences. Bayesian posterior probabilities greater than 90% are shown above the edges. Sequences for taxa marked # from Wang et al. (2006), the Chaetomella and Pilidium sequences are from Rossman et al. (2004), the remaining taxa sequenced as part of this study are listed in Table 1.

opennotspecifiedAug 2014View details →

FIGURE 2 in Clavatispora thailandica gen. et sp. nov., a novel taxon of Venturiales (Dothideomycetes) from Thailand

FIGURE 2. Sexual state of Clavatispora thailandica (MFLU100038, holotype). A. Substrate and ascomata appearance. B. Squash mount of ascoma. C. Section of ascoma. Note the fused hyphae forming hairs at the base. D. Setae. E. Peridium. F. Pseudoparaphyses. G–H. Immature bitunicate asci with young developing ascospores. I–K. Mature asci. Fissitunicate dehiscence arrowed in H and I. L. Characteristic dehiscence of asci after release of ascospores. M–P. Young and mature ascospores. Scale bars: A–C, G–L = 50 µm, D–E = 20 µm, F = 5 µm, M–P = 10 µm.

opennotspecifiedAug 2014View details →

FIGURE 1 in Clavatispora thailandica gen. et sp. nov., a novel taxon of Venturiales (Dothideomycetes) from Thailand

FIGURE 1. RAxML phylogenetic tree based on the LSU sequence data. Bootstrap support values for maximum likelihood (≥ 50% BS) and Bayesian posterior probabilities (≥ 0.95 PP) are shown in the tree (values below these thresholds not shown). The tree was rooted with Acrospermum compressum (Acrospermales) as outgroup. The new taxon is highlighted in blue and types are in bold.

FIGURE 5. CAPS assay for A in Aetokthonos hydrillicola gen. et sp. nov.: Epiphytic cyanobacteria on invasive aquatic plants implicated in Avian Vacuolar Myelinopathy

FIGURE 5. CAPS assay for A. hydrillicola. RsaI digestion of a PCR-amplified fragment of the16S rRNA gene produces a 247 bp sequence that is diagnostic for the species. At top, the sizes of RsaI digestion products from A. hydrillicola and related cyanobacteria are shown. Lane 1: RsaI digestion products of PCR-amplified DNA from an axenic culture of A. hydrillicola. Lane 2: PCR product from DNA of a field sample. Lane 3: RsaI digestion products of PCR-amplified DNA from a field sample. Arrowheads indicate the size (in base pairs) of the products of PCR and RsaI digestion. M = 100 bp DNA markers. Closed circles = PCR primer binding sites. Open circles = RsaI restriction sites.

FIGURE 1 in Aetokthonos hydrillicola gen. et sp. nov.: Epiphytic cyanobacteria on invasive aquatic plants implicated in Avian Vacuolar Myelinopathy

FIGURE 1. Locations (in order of confirmation) across the Southeastern United States where Aetokthonos hydrillicola, invasive plants (Hydrilla, Egeria, Myriophyllum), and bird deaths from AVM have been confirmed.

FIGURE 7 in Aetokthonos hydrillicola gen. et sp. nov.: Epiphytic cyanobacteria on invasive aquatic plants implicated in Avian Vacuolar Myelinopathy

FIGURE 7. Secondary structure of conserved domains in the 16S-23S ITS of true-branching Aetokthonos and Fischerella. A–C. D1–D1' helix, D–F. Box-B helix, G–I. V3 helix.

FIGURE 6 in Aetokthonos hydrillicola gen. et sp. nov.: Epiphytic cyanobacteria on invasive aquatic plants implicated in Avian Vacuolar Myelinopathy

FIGURE 6. Bayesian Analysis based on alignment of 318 OTU's. Posterior probabilities and bootstrap values from a parsimony analysis of the same alignment are shown above or in close proximity to the nodes that are supported at least at the 50% level. Asterisks (*) indicate 1.00 or 100% support, hyphens (-) indicate unsupported node. Nodes with no support from either analysis have no annotation. The six arrows indicate the clades in the tree consisting of true-branching taxa, and show that the former concept of Stigonematales or Section V

FIGURE 3 in Aetokthonos hydrillicola gen. et sp. nov.: Epiphytic cyanobacteria on invasive aquatic plants implicated in Avian Vacuolar Myelinopathy

FIGURE 3. Electron Microscope views of Aetokthonos hydrillicola. A–C. Scanning electron microscope views showing dehiscent apical caps (white arrows). D. Cells embedded in firm, thick, mucilage. Dark inclusions are likely cyanophycin bodies. E. Dehiscent apical cell showing initiation of dehiscence. F. Four thylakoids with characteristic electron dense and electron light areas, with dark areas associated with thylakoids representing phycobilisomes. G. Cell showing characteristic thylakoids, arrowed thylakoids shown at greater magnitude in Fig. 3F. H. High magnification views of cyanophycin and polyphosphate bodies.

FIGURE 2 in Aetokthonos hydrillicola gen. et sp. nov.: Epiphytic cyanobacteria on invasive aquatic plants implicated in Avian Vacuolar Myelinopathy

FIGURE 2. Light microscope views of Aetokthonos hydrillicola. A. Fluorescence microscopy of A. hydrillicola on leaf of Hydrilla, scale = 100 μm. B. Fluorescence microscopy of thallus morphology in culture, scale = 100 μm. C–D. Fluorescence microscopy of branching filaments, arrows show hormogonia production in sheath, Scale = 10 μm. E. Thallus showing apical caps (black arrow) and thick-walled enlarged cells possibly functioning as akinetes (white arrow), scale = 10 μm. F–G. Thallus showing typical branching pattern and dehiscent apical cells (hormocytes?) (arrows), scale=10 μm.

FIGURE 3 in Cyanocohniella calida gen. et sp. nov. (Cyanobacteria: Aphanizomenonaceae) a new cyanobacterium from the thermal springs from Karlovy Vary, Czech Republic

FIGURE 3. Phylogenetic position of the genus Cyanocohniella in the order Nostocales based on Bayesian analysis with 16S rRNA gene sequence data. Bootstrap support from Bayesian, maximum parsimony and maximum likelihood analysis reported above nodes respectively. Sequences generated in this study are in bold font.

FIGURE 2 in Cyanocohniella calida gen. et sp. nov. (Cyanobacteria: Aphanizomenonaceae) a new cyanobacterium from the thermal springs from Karlovy Vary, Czech Republic

FIGURE 2. Morphological variability of the type strain of Cyanocohniella calida sp. nov. A. Hormogonia (Pseudanabaena-like stadium). B. Intermediate stage between Pseudanabaena and Nostoc-like stages. C–G. Nostoc-like stage. H. Different sizes of cells in one trichome. I–J. Akinetes. K–N. Chlorogloeopsis-like stage, multiple brachning. O, P. Germination of akinetes. Scale = 5 μm

FIGURE 5. Box B and V3 in Cyanocohniella calida gen. et sp. nov. (Cyanobacteria: Aphanizomenonaceae) a new cyanobacterium from the thermal springs from Karlovy Vary, Czech Republic

FIGURE 5. Box B and V3 helices of the 16S-23S ITS for Cyanocohniella and comparison taxa (full citations to taxa and accession numbers given in legend for Fig. 4). A–G. Box B helix. H–N. V3 helix. Sequences of all comparison taxa are compared to sequence of C. calida and minimum number of mutations to achieve the C. calida sequence are given in circles above terminus of each structure. Possible substitutions (hollow circles), deletions (minus signs), and insertions (plus signs) are shown at positions in helix where they likely occurred if differences observed are explained parsimoniously through just comparison to C. calida.

FIGURE 4. D1–D1 in Cyanocohniella calida gen. et sp. nov. (Cyanobacteria: Aphanizomenonaceae) a new cyanobacterium from the thermal springs from Karlovy Vary, Czech Republic

FIGURE 4. D1–D1' helix of the 16S-23S ITS for Cyanocohniella and comparison taxa. A. Cyanocohniella calida CCALA 1049 (KJ737427). B. Dolichospermum circinale 33-10 (EF634474). C. Nodularia harveyana Hubel 1983/300 (AF367159). D. Cylindrospermum ovalisporum ILC-164 (JF768743). E. Anabaenopsis Oleksovice (KF010323), F. Camptylonemopsis sp. HA4241-MV5 (JN385292). G. Nostoc lichenoides CNP-AK1 (AY579894). Sequences of all comparison taxa are compared to sequence of Cyanocohniella calida and minimum number of mutations to achieve the C. calida sequence are given in circles above terminus of each structure. Possible substitutions (hollow circles) and insertions (plus signs) are shown at positions in helix where they likely occurred if differences observed are explained parsimoniously through just comparison to C. calida.

FIGURE 3 in Glebych minutissimus gen. et sp. nov., the smallest cobweb spider (Araneae Theridiidae)

FIGURE 3. SEM images of the male palp of Glebych minutissimus sp. n. A–C intact, meso-ventral, ecto-ventral and ecto-dorsal; D–I expanded palp, different views. Scale = 0.1 mm. Abbreviations: Co conductor, Cs cymbial spur, Eb embolic base, Em embolus, Ma median apophysis, Mp anterior process of median apophysis, Sm serrate retrolateral margin, Ss strong seta of Cs, Tp retrolateral pit of tegulum.

FIGURE 2 in Glebych minutissimus gen. et sp. nov., the smallest cobweb spider (Araneae Theridiidae)

FIGURE 2. SEM images of Glebych minutissimus sp. n., male (A–E, G–I) and female (F, J–K). A–B prosoma, lateral and frontal; C eye group; D spinnerets and colulus; E posterior part of abdomen; F–H chelicera; I terminal part of cymbium with removed bulb showing cymbial spoor; J–K epigyne, ventro-lateral and antero-ventral. Scale = 0.1 mm, unless otherwise indicated. Abbreviations: Cl colulus, Cs cymbial spur, Ep epigynal plate, MAP major ampulate gland spigot, Me median plate of epigyne, Oc copulatory opening, Ss strong seta of cymbial spur, Ts tracheal spiracle.

FIGURE 1 in Glebych minutissimus gen. et sp. nov., the smallest cobweb spider (Araneae Theridiidae)

FIGURE 1. General appearance of Glebych minutissumus sp. n. of the male (A–D) and female (E–F). A prosoma, dorsal; B, E body, lateral; C, F body, ventral; D prosoma, dorso-lateral. Scale = 0.2 mm. Abbreviation: Ts tracheal spiracle.

FIGURE 4 in Glebych minutissimus gen. et sp. nov., the smallest cobweb spider (Araneae Theridiidae)

FIGURE 4. Glebych minutissimus sp. n., male (A, B, D–G) and female (C, H–L). A prosoma, frontal; B tarsus IV; C palp; D leg I; E abdomen; F–G epiandrus (two individuals); H epigyne, ventral; I–J epigyne, antero-ventral; K epigyne and uterus internus, antero-ventral; L abdomen, antero-ventral. Scale = 0.1 mm. Abbreviations: Cd copulatory duct, Ea epiandrus, Gs gland spigot, Me median plate of epigyne, Oc copulatory opening Re receptacle, To tarsal organ.

opennotspecifiedOct 2017View details →

Figure 14 in Thalamocyclops pachypes gen. nov., sp. nov. (Copepoda: Cyclopoida: Cyclopidae), a crevicular cyclopine from Socotra Island (Yemen): tale of a remarkable survival drive

Figure 14. Thalamocyclops soqotraensis (Mirabdullayev et al., 2002) comb. nov. (a) Female urosome, dorsal; (b) female abdominal somites, ventral; (c) male urosome, ventral (illustrations from type series, RBINSc COP 4492–4495).