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739 results for “Printing”
Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC)
<p>Supplementary Videos, CAD 3D-printing templates and assembly instructions</p>
Entorhino-hippocampal slice culture on 3D-printed silk fibroin insert
<p>20230406_LM_slice_culture: A TIF-file showing an LM-image (Carl Zeiss-Stemi 305 - 3.0X) of cultured entorhino-hippocampal slice cultures (22 days) on the metal frame spider silk.</p> <p>20230426_CM_slice_culture: a TIF-file showing an LSM-image (Leica TCS SP8, 20X (NA 0.75;Leica)) of PI and DAPI staining of hippocampal-entorhinal cortical slices (22 days in vitro) on the metal frame spider silk.</p> <p>20230522_LM_slice_culture01: A TIF-file showing an LM-image (Carl Zeiss-Stemi 305 - 2.0X) of entorhino-hippocampal slice culture (7 days in vitro) on the 3D-printed insert.</p> <p>20230627_LM_slice_culture02: A TIF-file showing an LM-image (Carl Zeiss-Stemi 305 - 4.0X) of entorhino-hippocampal slice culture (5 days invitro) on the 3D-printed insert.</p>
Recapitulating and Deciphering Tumor-stroma Microenvironment by Using a “LEGO” like 3D printed microfluidic device
GEO Series GSE121477. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
3D-Printed PCL Scaffolds Loaded with bFGF and BMSCs(PCLMF) Enhance Tendon-Bone Healing in Rat Rotator Cuff Tears by Immunomodulation and Osteogenesis Promotion
GEO Series GSE277270. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
3D-printed Scaffolds to Regulate the Translational Balance between Vascularization and Bone Metabolism
GEO Series GSE246631. Homo sapiens; Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
Electro-assisted cell printing directs in vitro gastrulation and epiblast-like morphogenesis from human pluripotent stem cells II
GEO Series GSE294833. Homo sapiens. 4 samples. Type: Expression profiling by high throughput sequencing.
Data set from the article Giamberti A, Chessa M, Chiarello C, Cipriani A, Carotti A, Galletti L, Gargiulo G, Marianeschi SM, Pace Napoleone C, Padalino M, Perri G, Luciani GB. Italian survey on cardiac surgery for adults with congenital heart disease: which surgery, where and by whom? Interact Cardiovasc Thorac Surg. 2019 Mar 25:ivz045. doi: 10.1093/icvts/ivz045. Epub ahead of print. PMID: 30907407.
<p>Data set from the article Giamberti A, Chessa M, Chiarello C, Cipriani A, Carotti A, Galletti L, Gargiulo G, Marianeschi SM, Pace Napoleone C, Padalino M, Perri G, Luciani GB. Italian survey on cardiac surgery for adults with congenital heart disease: which surgery, where and by whom? Interact Cardiovasc Thorac Surg. 2019 Mar 25:ivz045. doi: 10.1093/icvts/ivz045. Epub ahead of print. PMID: 30907407.</p> <p>This is the abstract</p> <p><strong>Objectives: </strong>The population of ageing adults with congenital heart disease (ACHD) is increasing; surgery in these patients presents major difficulties in management. A great debate has developed about whether these patients should be cared for at an adult or paediatric hospital and by an acquired or congenital cardiac surgeon. We analysed data of the surgical treatment of ACHD from the Italian cardiac surgery centres in 2016, focusing on the type of surgery performed, where these patients were operated on and by whom.</p> <p><strong>Methods: </strong>Ninety-two Italian cardiac surgery centres were contacted and 70 centres participated in this study. We collected data on the types of cardiac operations performed in congenital heart defect patients older than 18 years. In 2016, a total of 913 patients with ACHD were operated on: 440 by congenital cardiac surgeons (group I) in centres with paediatric and adult cardiac surgery units, and 473 by adult cardiac surgeons (group II) in centres with exclusively adult cardiac surgery units.</p> <p><strong>Results: </strong>Pathologies of the right ventricular outflow tract were the most frequent diseases treated in group I and pathologies of the left ventricular outflow tract in group II. Group I included more complex and heterogeneous cases than group II. Surgery for ACHD represented 12% of the activity of congenital cardiac surgeons and only 1% of the activity of adult cardiac surgeons.</p> <p><strong>Conclusions: </strong>In Italy, ACHD patients are operated on both by congenital and adult cardiac surgeons. Congenital cardiac surgeons working in centres with both paediatric and adult cardiac surgery are more involved with ACHD patients and with more complex cases.</p>
Data set from Fantini F, Toso A, Menicanti L, Moroni F, Castelvecchio S. Restrictive filling pattern in ischemic cardiomyopathy: Insights after surgical ventricular restoration. J Thorac Cardiovasc Surg. 2019 Oct 30:S0022-5223(19)32349-9. doi: 10.1016/j.jtcvs.2019.09.173. Epub ahead of print. PMID: 31767363.
<p>Data set from Fantini F, Toso A, Menicanti L, Moroni F, Castelvecchio S. Restrictive filling pattern in ischemic cardiomyopathy: Insights after surgical ventricular restoration. J Thorac Cardiovasc Surg. 2019 Oct 30:S0022-5223(19)32349-9. doi: 10.1016/j.jtcvs.2019.09.173. Epub ahead of print. PMID: 31767363.</p> <p> </p> <p>This is the abstract:</p> <p><strong>Objective: </strong>To examine factors possibly involved in the resolution or persistence of restrictive filling pattern (RFP) after surgical ventricular restoration (SVR) in a series of patients with ischemic cardiomyopathy (ICM) and RFP.</p> <p><strong>Methods: </strong>Echocardiography was performed at baseline (pre-SVR), discharge, and follow-up in 43 patients with ICM and RFP (E/A ratio ≥2). Patients were divided into 2 groups based on E/A ratio at discharge: improved (E/A ratio <2; 22 patients) and unchanged (E/A ratio ≥2; 21 patients).</p> <p><strong>Results: </strong>The improved group had a significantly increased mean deceleration time (from 137 ± 22 ms to 194 ± 68 ms; P = .002) and mean A wave velocity (from 43 ± 10 cm/s to 92 ± 37 cm/s; P = .001), and decreased E/e' ratio (from 27.7 ± 9.5 to 19.2 ± 7.8; P = .01) after SVR. The unchanged group did not show any significant variations in diastolic parameters. The only significant differences at baseline between the two groups were thinner left ventricle posterior wall and lower relative wall thickness (RWT) in the unchanged group. RWT was the sole baseline parameter independently associated with persistent RFP.</p> <p><strong>Conclusions: </strong>RFP was reversed after SVR in 22 of our 43 patients with ICM with a response that remained stable over time, associated with improved New York Heart Association class. RWT was the sole baseline echocardiographic parameter significantly associated with the evolution of RFP after</p>
Data set and 3d model from Emendi M, Sturla F, Ghosh RP, Bianchi M, Piatti F, Pluchinotta FR, Giese D, Lombardi M, Redaelli A, Bluestein D. Patient-Specific Bicuspid Aortic Valve Biomechanics: A Magnetic Resonance Imaging Integrated Fluid-Structure Interaction Approach. Ann Biomed Eng. 2020 Aug 17. doi: 10.1007/s10439-020-02571-4. Epub ahead of print. PMID: 32804291.
<p>Data set and 3d model from Emendi M, Sturla F, Ghosh RP, Bianchi M, Piatti F, Pluchinotta FR, Giese D, Lombardi M, Redaelli A, Bluestein D. Patient-Specific Bicuspid Aortic Valve Biomechanics: A Magnetic Resonance Imaging Integrated Fluid-Structure Interaction Approach. Ann Biomed Eng. 2020 Aug 17. doi: 10.1007/s10439-020-02571-4. Epub ahead of print. PMID: 32804291.</p> <p> </p> <p>This is the abstract:</p> <p>Congenital bicuspid aortic valve (BAV) consists of two fused cusps and represents a major risk factor for calcific valvular stenosis. Herein, a fully coupled fluid-structure interaction (FSI) BAV model was developed from patient-specific magnetic resonance imaging (MRI) and compared against in vivo 4-dimensional flow MRI (4D Flow). FSI simulation compared well with 4D Flow, confirming direction and magnitude of the flow jet impinging onto the aortic wall as well as location and extension of secondary flows and vortices developing at systole: the systolic flow jet originating from an elliptical 1.6 cm<sup>2</sup> orifice reached a peak velocity of 252.2 cm/s, 0.6% lower than 4D Flow, progressively impinging on the ascending aorta convexity. The FSI model predicted a peak flow rate of 22.4 L/min, 6.7% higher than 4D Flow, and provided BAV leaflets mechanical and flow-induced shear stresses, not directly attainable from MRI. At systole, the ventricular side of the non-fused leaflet revealed the highest wall shear stress (WSS) average magnitude, up to 14.6 Pa along the free margin, with WSS progressively decreasing towards the belly. During diastole, the aortic side of the fused leaflet exhibited the highest diastolic maximum principal stress, up to 322 kPa within the attachment region. Systematic comparison with ground-truth non-invasive MRI can improve the computational model ability to reproduce native BAV hemodynamics and biomechanical response more realistically, and shed light on their role in BAV patients' risk for developing complications; this approach may further contribute to the validation of advanced FSI simulations designed to assess BAV biomechanics.</p> <p> </p>
Data_Indirect 3D Printed Electrode Mixers
<p>The folder contains the processed data of figures 4, 5, 6, S2 and S3 that appear in:</p> <p>J. Hereijgers, J. Schalck, J. Lölsberg, M. Wessling, T. Breugelmans, Indirect 3D Printed Electrode Mixers, ChemElectroChem. 6 (2019) 378–382. <a href="https://doi.org/10.1002/celc.201801436">https://doi.org/10.1002/celc.201801436</a>.</p>
Dataset related to article "Frozen Section Analysis and Real-Time Magnetic Resonance Imaging of Surgical Specimen Oriented on 3D Printed Tongue Model to Assess Surgical Margins in Oral Tongue Carcinoma: Preliminary Results"
<p>This record contains raw data related to article “Frozen Section Analysis and Real-Time Magnetic Resonance Imaging of Surgical Specimen Oriented on 3D Printed Tongue Model to Assess Surgical Margins in Oral Tongue Carcinoma: Preliminary Results"</p> <p>Abstract</p> <p><strong>Background: </strong> A surgical margin is the apparently healthy tissue around a tumor which has been removed. In oral cavity carcinoma, a negative margin is considered ≥ 5 mm, a close margin between 1 and 5 mm, and a positive margin ≤ 1 mm. Currently, the intraoperative surgical margin status is based on the visual inspection and tissue palpation by the surgeon and intraoperative histopathological assessment of the resection margins by frozen section analysis (FSA). FSA technique is limited and susceptible to sampling errors. Definitive information on the deep resection margins requires postoperative histopathological analysis.</p> <p><strong>Methods: </strong> We described a novel approach for the assessment of intraoperative surgical margins by examining a surgical specimen oriented through a 3D-printed specific patient tongue with real-time Magnetic Resonance Imaging (MRI). We reported the preliminary results of a case series of 10 patients, prospectively enrolled, with oral tongue carcinoma who underwent surgery between February 2020 and April 2021. Two radiologists with 5 and 10 years of experience, respectively, in Head and Neck radiology in consensus evaluated specimen MRI and measured the distance between the tumor and the specimen surface. We performed intraoperative bedside FSA. To compare the performance of bedside FSA and MRI in predicting definitive margin status we computed the weighted sensitivity (SE), specificity (SP), accuracy (ACC), area under the ROC curve (AUC), F1-score, Positive Predictive Value (PPV), and Negative Predictive Value (NPV). To express the concordance between FSA and <em>ex-vivo</em> MRI we reported the jaccard index.</p> <p><strong>Results: </strong> Intraoperative bedside FSA showed SE of 90%, SP of 100%, F1 of 95%, ACC of 0.9%, PPV of 100%, NPV (not a number), and jaccard of 90%, and <em>ex-vivo</em> MRI showed SE of 100%, SP of 100%, F1 of 100%, ACC of 100%, PPV of 100%, NPV of 100%, and jaccard of 100%. These results needed to be validated in a larger sample size of 21- 44 patients.</p> <p><strong>Conclusion: </strong> The presented method allows a more accurate evaluation of surgical margin status, and the first clinical experiences underline the high potential of integrating FSA with <em>ex-vivo</em> MRI of the fresh surgical specimen.</p>
Expression data from 3D cell-printed human bone marrow derived mesenchymal stem cells (hBMMSCs) with liver, heart, skin and cornea decellularized extracellular matrix (dECM) bioinks
GEO Series GSE126877. Homo sapiens. 8 samples. Type: Expression profiling by array.
Perfusion of the 3D printed construct after removal of the sacrificial ink (8x8x6mm)..
<p>We used silated-hydroxypropylmethylcellulose (Si-HPMC) hydrogel, a cellulose ether derivative, that has been used in cartilage, bone tissue engineering and was demonstrated to be biocompatible. Si-HPMC hydrogel crosslinking is promoted by pH neutralization, which makes it a good candidate for bioprinting as it is a cytocompatible process and has a printing time window of about 30 minutes during which the gel viscosity increases. Herein, a thick structure with perfusable channels and cell-laden Si-HPMC was bioprinted.The construct was perfused with a dye solution. It was observed that the dye solution occupied all the volume of the hollow microchannels (MOVIE 1 in supplementary), suggesting that there was no obstruction of microchannels and all channels were in fact interconnected. </p>
Data set from Spinelli D, Marconi S, Caruso R, Conti M, Benedetto F, De Beaufort HW, Auricchio F, Trimarchi S. 3D printing of aortic models as a teaching tool for improving understanding of aortic disease. J Cardiovasc Surg (Torino). 2019 Oct;60(5):582-588. doi: 10.23736/S0021-9509.19.10841-5. Epub 2019 Jun 26. PMID: 31256581.
<p>Data set from Spinelli D, Marconi S, Caruso R, Conti M, Benedetto F, De Beaufort HW, Auricchio F, Trimarchi S. 3D printing of aortic models as a teaching tool for improving understanding of aortic disease. J Cardiovasc Surg (Torino). 2019 Oct;60(5):582-588. doi: 10.23736/S0021-9509.19.10841-5. Epub 2019 Jun 26. PMID: 31256581.</p> <p> </p> <p>This is the abstract:</p> <p><strong>Background: </strong>A geometrical understanding of the individual patient's disease morphology is crucial in aortic surgery. The aim of our study was to validate a questionnaire addressing understanding of aortic disease and use this questionnaire to investigate the value of 3D printing as a teaching tool for surgical trainees.</p> <p><strong>Methods: </strong>Anonymized CT-angiography images of six different patients were selected as didactic cases of aortic disease and made into 3D models of transparent rigid resin with the Vat-photopolymerization technique. The 3D aortic models, which could be disassembled and reassembled, were displayed to 37 surgical trainees, immediately after a seminar on aortic disease. A questionnaire was developed to compare the trainees' understanding before (T0) and after (T1) demonstration of the 3D printed models.</p> <p><strong>Results: </strong>A panel of 15 experts participated in evaluating face and content validity of the questionnaire. The questionnaire validity was established and therefore the information investigated by the questionnaire could be synthetized using the mean of the items to indicate the understanding. The participants (mean age 28 years, range 26-34, male 59%) showed a significant improvement in understanding from T0 (median=7.25; IQR=1.50) to T1 (median=8.00; IQR=1.50; P=0.002).</p> <p><strong>Conclusions: </strong>Preliminary data suggest that the use of 3D-printed aortic models as a teaching tool was feasible and improved the understanding of aortic disease among surgical trainees.</p>
Modification of polyurethane materials with fluorine and siloxane groups for use as biomedical materials in 3D printing - experimental data
<p>Experimental data collected as part of the research project "Modification of polyurethane materials with fluorine and siloxane groups for use as biomedical materials in 3D printing" (grant no. 2022/06/X/ST4/00452) financed by the National Science Center (NCN) as part of the MINIATURA 6 program.</p>
Cytotoxicity and estrogenicity of 3D printed orthodontic aligners
<p>Dataset for all analyses</p>
The effect of printing orientation on mechanical properties of 3D-printed orthodontic aligners
<p>Dataset for all analyses.</p>
Effect of heat treatment and Nitrogen atmosphere during post curing on mechanical properties of 3D printed orthodontic aligners
<p>Dataset for all analyses</p>
Dataset for manuscript "Cactus-inspired design principles for soft robotics based on 3D printed hydrogel-elastomer systems"
<p>The dataset includes data for the article "Cactus-inspired design principles for soft robotics based on 3D printed hydrogel-elastomer systems", DOI: 10.1016/j.matdes.2021.109515</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.