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opennotspecifiedJul 2021View details →

Fig. 5 in A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina

Fig. 5. Effects of incubation at different temperatures and pH on the activity of AmSOD1. The purified AmSOD1 was incubated (A) at temperatures (25, 37, 50, 60 and 75 ◦ C) (B) in buffers with pH (3–12) for 1 h before SOD assay. Then the activity of AmSOD1 was determined as explained in the "Materials and Methods". Each data point represents the mean ± SD obtained from three independent reactions.

opencc-by-4.0Aug 2023View details →

Repeatome turnover meets stable chromosomes: repetitive DNA sequences mark speciation and gene pool boundaries in sugar beet and wild beets

The present repository provides zipped archives containing the results of the RepeatExplorer2 runs of individual as well as comparative repeat analyses in beet genomes.   Sugar beet (Beta vulgaris subsp. vulgaris) and its crop wild relatives share a base chromosome number of nine and similar chromosome morphologies. Yet, interspecific breeding is impeded by chromosome and sequence divergence that is still not fully understood. Since repetitive DNA sequences represent the fastest evolving parts of the genome, they likely impact genomic variability and contribute to the separation of beet gene pools. Hence, we investigated if innovations and losses in the repeatome can be linked to chromosomal differentiation and speciation. We traced genome- and chromosome-wide evolution across sugar beet and twelve wild beets comprising all sections of the beet genera Beta and Patellifolia. For this, we combined data from short and long read sequencing, flow cytometry, and cytogenetics to build a comprehensive data framework for our beet panel that spans the complete scale from DNA sequence to chromosome up to the genome. Genome sizes and repeat profiles reflect the separation of the beet species into three gene pools. These gene pools harbor repeats with contrasting evolutionary patterns: We identified section- and species-specific repeat emergences and losses, e.g. of the retrotransposons causal for genome expansions in the section Corollinae/Nanae. Since most genomic variability was found in the satellite DNAs, we focused on tracing the 19 beetSat families across the three beet sections/genera. These taxa harbor evidence for contrasting strategies in repeat evolution, leading to contrasting satellite DNA profiles and fundamentally different centromere architectures, ranging from chromosomal uniformity in Beta and Patellifolia species to the formation of patchwork chromosomes in Corollinae/Nanae species.  We show that repetitive DNA sequences are causal for genome size expansion and contraction across the beet genera, providing insights into the genomic underpinnings of beet speciation. Satellite DNAs in particular vary considerably among beet taxa, leading to the evolution of distinct chromosomal setups. These differences likely contribute to the barriers in beet breeding between the three gene pools. Thus, with their isokaryotypic chromosome sets, beet genomes present an ideal system for studying the link between repeats, genome variability, and chromosomal differentiation/evolution and provide a theoretical basis for understanding barriers in crop breeding.

Fig. 4. 1H in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 4. 1H NMR spectra (400 MHz, D O) of a crab apple extract fraction (I) and sucrose (II). The circled signal was consistent with the anomeric proton of a β-AAG.

Fig. 7 in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 7. Acetylation of 2 under basic conditions gave the unsaturated glycoside 8, but acidic conditions led to the successful acetylation of both 2 and 2a. I. Ac2O, pyridine, 0 ̊C, overnight, 44%. II. Ac2O, HClO4, 0 ̊C, 1 h, 71% (9) and 73% (9a) yield.

Fig. 1 in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 1. Structures of AA (1) and the only known naturally occurring derivatives in plants, 2-O-β-D-glucopyranosyl L-ascorbic acid (2) and 6-O-β-D-glucopyranosyl L-ascorbic acid (3). Synthetic 2-O-α-D-glucopyranosyl L-ascorbic acid (4) is commonly used in cosmetic products.

Fig. 2 in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 2. Left: PCA (Pareto scaling with 95% confidence interval) of the LC-MS analyses of extracts from four fruit of each of nine apple accessions: green triangles = M. sylvestris, red circles = M. domestica, purple Xs = M. sieversii. Right: PCA of molecular features showing that M. domestica accessions were characterised by polyphenols (red), M. sieversii by fatty acids (blue) and M. sylvestris by dihydrochalcones (green). A molecular feature tentatively identified as an ascorbyl glycoside (top left) was also associated with two M. sylvestris accessions. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 3 in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 3. LC-MS chromatograms (Instrument B, method I) of crab apple extract, commercial 2-O-α-D-glucopyranosyl L-ascorbic (4), and two AAGs produced by chemical synthesis, 2-O-β-D-glucopyranosyl L-ascorbic (2) and 2-O-β-D-galactopyranosyl L-ascorbic (2a). The traces are extracted ion chromatograms for m/z 337 [M – H]-. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 9 in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 9. LC-MS (Instrument B, method II) extracted ion chromatograms for m/z 655, [M + Na]+, of acetylated AAGs 9 (red, 7.0 min) and 9a (green, 8.1 min), and acetylated and purified crab apple AAG (blue, 7.0 min). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 8 in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 8. The downfield regions of the 1H NMR spectra for acetylated ascorbyl glycosides (structures in Fig. 7.). I. Peracetyl galactoside 9a, II. Acetylated and purified ascorbyl glycoside from crab apples, III. Peracetyl glucoside 9.

Fig. 6. 1 H in Discovery of a stable vitamin C glycoside in crab apples (Malus sylvestris)

Fig. 6. 1 H NMR spectra of β-glucoside 2 in D O (400 MHz) demonstrating the effect of pH on proton resonances 1′-H and 4-H. Top: one drop of 2 M NaOH added to 2 NMR tube; middle: nothing added; bottom: one drop of conc. HCl added to NMR tube.

opencc-by-4.0Sep 2023View details →

Dataset and analyses for: Mediolateral foot placement control can be trained: Older adults learn to walk more stable, when ankle moments are constrained

Here you'll find the dataset and analyses belonging to the publication "Mediolateral foot placement control can be trained: Older adults learn to walk more stable, when ankle moments are constrained". Older adults performed 8 sessions of treadmill walking, of which 6 sessions comprised of a training on a shoe constraining ankle moment control (LesSchuh). We evaluated whether participants improved their foot placement control and gait stability as a result of the training. Kinematic data of the feet and thorax were collected. For more detail on the folders' contents please see "Read me.docx".

opencc-by-4.0Oct 2023View details →

Stable sound decoding despite modulated sound representation in the auditory cortex

Two-photon calcium imaging data (df/f) from the mouse auditory cortex during a two-alternative choice auditory decision-making task. This is the data set of the paper titled: "Stable sound decoding despite modulated sound representation in the auditory cortex" Current Biology 33, 1–14, October 23, 2023 DOI:<a href="https://doi.org/10.1016/j.cub.2023.09.031">https://doi.org/10.1016/j.cub.2023.09.031</a>

opencc-by-4.0Jan 2019View details →

Data: Two-sided profile-based optimality in the stable marriage problem

This data corresponds to the data and experiments described in Section 5 of the following paper: Two-sided profile-based optimality in the stable marriage problem Authors: Frances Cooper and David Manlove <ul> <li>The paper is located at: <a href="https://arxiv.org/abs/1905.06626">https://arxiv.org/abs/1905.06626</a></li> <li>The data is located at: <a href="https://doi.org/10.5281/zenodo.2542703">https://doi.org/10.5281/zenodo.2542703</a></li> <li>The software is located at: <a href="https://doi.org/10.5281/zenodo.1183221">https://doi.org/</a><a href="https://doi.org/10.5281/zenodo.2545798">10.5281/zenodo.2545798</a></li> </ul> See the README for more information. Version 1.0.2 updates: * Updated README

Efficacy of Ciclesonide on Small Airways in Patients With Stable Asthma (18 to 60 y) (BY9010/M1-131)

ClinicalTrials.gov study NCT00163345. IPD Sharing: Not stated. Countries: 1. Publications: 1.

closedIPD-NOFeb 2026View details →

Operations Research of the 'Real World' Effectiveness of Multi-Month Dispensing of ART for Stable Patients in CARGs in Zimbabwe

ClinicalTrials.gov study NCT03238846. IPD Sharing: NO. Countries: 1. Publications: 4.

Efficacy and Safety of Treatment With Balaglitazone in Type 2 Diabetes Patients on Stable Insulin Therapy

ClinicalTrials.gov study NCT00515632. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Safety, Pharmacokinetic, and Pharmacodynamic Study of NKTT120 in Adult Patients With Stable Sickle Cell Disease (SCD)

ClinicalTrials.gov study NCT01783691. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Phase III Trial of Dantonic® (T89) Capsule to Prevent and Treat Stable Angina

ClinicalTrials.gov study NCT01659580. IPD Sharing: Not stated. Countries: 7. Publications: 1.

Regorafenib, Ipilimumab and Nivolumab for the Treatment of Chemotherapy Resistant Microsatellite Stable Metastatic Colorectal Cancer

ClinicalTrials.gov study NCT04362839. IPD Sharing: Not stated. Countries: 1. Publications: 1.