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Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/08/04
<p><strong>Project</strong> - Huntingtin structure-function open lab notebook. </p> <p><strong>Rationale </strong>- Purified huntingtin samples with and without stabilising binding partners (HAP40) are required for use in structural and functional studies, in particular SAXS experiments. </p>
FIG. 1. — Souzalopesmyia polleti n in Souzalopesmyia Albuquerque, 1951 (Diptera: Muscidae): new species from South America with an updated phylogeny based on morphological evidence, in Touroult J. (ed.), "Our Planet Reviewed" 2015 large-scale biotic survey in Mitaraka, French Guiana.
FIG. 1. — Souzalopesmyia polleti n. sp.: A-D, ♂ holotype: head, anterior view (A); head, lateral view (B); lateral view (C); dorsal view (D); E-H, ♀ paratype: head anterior view (E); head lateral view (F); lateral view (G); dorsal view (H). Scale bars: 1.0 mm.
FIG. 4 in Souzalopesmyia Albuquerque, 1951 (Diptera: Muscidae): new species from South America with an updated phylogeny based on morphological evidence, in Touroult J. (ed.), "Our Planet Reviewed" 2015 large-scale biotic survey in Mitaraka, French Guiana.
FIG. 4. — Geographical distribution of the species of Souzalopesmyia Albuquerque,1951 from South America, with phylogenetic tree superimposed. Symbols: ●, S. sulina de Carvalho, 1999; ●, S. carioca Albuquerque, 1951; ●, S. singularis (Stein, 1911); ●, Souzalopesmyia polleti n. sp.; ●, S. paraensis de Carvalho, 1999; ●, S. amazonica Albuquerque, 1951;, Souzalopesmyia sp.
FIG. 3 in Souzalopesmyia Albuquerque, 1951 (Diptera: Muscidae): new species from South America with an updated phylogeny based on morphological evidence, in Touroult J. (ed.), "Our Planet Reviewed" 2015 large-scale biotic survey in Mitaraka, French Guiana.
FIG. 3. — Most parsimonious phylogenetic hypothesis obtained from the analysis of a morphological data set of the six species of Souzalopesmyia and the three outgroups species (Helina praecipua (Walker, 1853), Phaonia praesuturalis (Stein, 1904) and Dolichophaonia trigona (Shannon & Del Ponte, 1926)). All characters were given the same weight and treated as unordered. Tree length: 34, consistency index: 0.82, retention index: 0.84. Filled circles represent unique transforma- tion, and empty circles represent homoplastic character state transformation under fast optimization. Characters and character states: see Appendix.
FIGURE 4. Forewing a. scales b. fringes, drawing T in Eumasia thomasii sp. nov. a new species of the subfamily Eumasiinae (Lepidoptera: Psychidae) from India
FIGURE 4. Forewing a. scales b. fringes, drawing T. Sobczyk
Supplementary material 1 from: Patterson DJ (2022) The scope and scale of the life sciences ('Nature's envelope'). Research Ideas and Outcomes 8: e96132. https://doi.org/10.3897/rio.8.e96132
Patterson Nature's envelope (template)
Climate and geology overwrite land use effects on soil organic nitrogen cycling on a continental scale
<p><strong>Abstract.</strong> Soil fertility and plant productivity are globally constrained by N availability. Proteins are the largest N reservoir in soils and the cleavage of proteins into small peptides and amino acids has been shown to be the rate limiting step in the terrestrial N cycle. However, we are still lacking a profound understanding of the environmental controls of this process. Here we show that integrated effects of climate and soil geochemistry drive protein cleavage across large scales. We measured gross protein depolymerization rates in mineral and organic soils sampled across a 4000-km-long European transect covering a wide range of climates, geologies and land uses. Based on structural equation models we identified that soil organic N cycling was strongly controlled by substrate availability, e.g. by soil protein content. Soil geochemistry was a secondary predictor, by controlling protein stabilization mechanisms and protein availability. Precipitation was identified as the main climatic control on protein depolymerization, by affecting soil weathering and soil organic matter accumulation. In contrast, land use was a poor predictor of protein depolymerization. Our results highlight the need to consider geology and precipitation effects on soil geochemistry when estimating and predicting soil N cycling at large scales.</p>
Fig. ¹. 7KRWWHD EHXQJRQJWDQRHK MustaqimNJ ANJ Living plantNJ BNJ Leaf, adaxial sideNJ CNJ Leaf, abaxial sideNJ DNJ InflorescencesNJ ENJ Flower, lateral viewNJ FNJ Flower, top viewNJ GNJ Perianth, outer surfaceNJ HNJ Perianth, inner surfaceNJ INJ StamensNJ JNJ Style and stigmaNJ KNJ Immature capsuleNJ Scale bars: B-C, F = 1 cm, D-E = 1 cm, G = 5 mm, H-J = 1 mm, K = 5 mmNJ Photographed by Wendy ANJ Mustaqim in Thottea beungongtanoeh (Aristolochiaceae), a new species Irom Aceh, northern Sumatra
Fig. ¹. 7KRWWHD EHXQJRQJWDQRHK MustaqimNJ ANJ Living plantNJ BNJ Leaf, adaxial sideNJ CNJ Leaf, abaxial sideNJ DNJ InflorescencesNJ ENJ Flower, lateral viewNJ FNJ Flower, top viewNJ GNJ Perianth, outer surfaceNJ HNJ Perianth, inner surfaceNJ INJ StamensNJ JNJ Style and stigmaNJ KNJ Immature capsuleNJ Scale bars: B-C, F = 1 cm, D-E = 1 cm, G = 5 mm, H-J = 1 mm, K = 5 mmNJ Photographed by Wendy ANJ Mustaqim
Data and software for "Multi-scale variability of turbulent mixing during a Monsoon Intraseasonal Oscillation in the Bay of Bengal: an LES study"
<p>LES data outputs</p>
Landscape structure affects metapopulation-scale tipping points
<p>Even when environments deteriorate gradually, ecosystems may shift abruptly from one state to another. Such catastrophic shifts are difficult to predict and sometimes to reverse (so-called hysteresis). While well studied in simplified contexts, we lack a general understanding of how catastrophic shifts spread in realistically spatially structured landscapes. For different types of landscape structures, including typical terrestrial modular and riverine dendritic networks, we here investigate landscape-scale stability in metapopulations whose patches can locally exhibit catastrophic shifts. We find that such metapopulations usually exhibit large-scale catastrophic shifts and hysteresis and that the properties of these shifts depend strongly on the metapopulation spatial structure and on the population dispersal rate: an intermediate dispersal rate, a low average degree or a riverine spatial structure can largely reduce hysteresis size. Our study suggests that large-scale restoration is easier with spatially clustered restoration efforts and in populations characterized by an intermediate dispersal rate.</p>
Data attached to Radio Science paper "Model to Scale Rain Attenuation Time Series with Link Elevation Angle for LEO Satellite Based Systems"
<p>Data attached to Radio Science paper "Model to Scale Rain Attenuation Time Series with Link Elevation Angle for LEO Satellite Based Systems"</p>
Data files used in the paper "First Radar Evidence of Large-Scale Englacial Folding in the South Polar Layered Deposits (Ultimi Scopuli, Mars) and Possible Ice Sheet Flow Unveiled by MARSIS" By Guallini et al.
<p>This archive contains radargrams, geometric information and visualizations of a subset of MARSIS radar observations over Planum Australe, Mars. This archive contains everything needed to reproduce the results presented in the paper "Large-scale folds detected by MARSIS in the Southern ice sheet of Ultimi Scopuli (Mars)" by Guallini et al.</p> <p>Three types of MARSIS data files are contained in this archive:</p> <p>* orbit_XXXXX_frequency_Y_MHz_radargram.csv, where XXXXX is the orbit number, and Y the frequency at which the radar was operating, in MHz. The file contains an ASCII table of real numbers separated by commas. The table has as many columns as the number of radar echoes acquired during the orbit (usually 3200), and 980 lines, one for each echo sample. Values are samples of the uncalibrated echo voltage, without phase information (i.e. positive real numbers instead of complex echo samples). Echo samples are acquired every 0.3571 microseconds (2.8 MHz sampling rate). The first sample of an echo is located at a round-trip time corresponding to an altitude of 25 km above the Martian IAU ellipsoid.</p> <p>* orbit_XXXXX_frequency_Y_MHz_geometry.csv, where XXXXX is the orbit number, and Y the frequency at which the radar was operating, in MHz. The file contains an ASCII table of real numbers separated by commas. The table has as many rows as the number of radar echoes acquired during the orbit (usually 3200), and contains the following auxiliary parameters:</p> <p> - EPHEMERIS TIME - Number of seconds elapsed since Jan 1, 2000, 12:00 UTC corresponding to the time at which data collection for the current echo started.</p> <p> - MARS SOLAR LONGITUDE - Angle between the Mars-Sun line at the time corresponding to EPHEMERIS TIME and the Mars-Sun line at the vernal equinox, in degrees.</p> <p> - MARS SUN DISTANCE - Distance from the centre of Mars to centre of the Sun at the time corresponding to EPHEMERIS TIME, in Km.</p> <p> - SPACECRAFT ALTITUDE - Distance from the Mars Express spacecraft to the reference surface of the target body measured normal to the surface at the time corresponding to EPHEMERIS TIME, expressed in Km.</p> <p> - SUB-SPACECRAFT LONGITUDE - East longitude of the point on the target body that lies closest to the Mars Express spacecraft at the time corresponding to EPHEMERIS TIME, expressed in degrees and in the [ 0 -360 ] range.</p> <p> - SUB-SPACECRAFT LATITUDE - Planetocentric latitude of the point on the target body that lies directly beneath the Mars Express spacecraft at the time corresponding to EPHEMERIS TIME, expressed in degrees.</p> <p> - RADIAL VELOCITY - Radial component of the Mars Express spacecraft velocity vector in the reference frame of the target body at the time corresponding to EPHEMERIS TIME, expressed in Km/s.</p> <p> - TANGENTIAL VELOCITY - Tangential component of the Mars Express spacecraft velocity vector in the reference frame of the target body at the time corresponding to EPHEMERIS TIME, expressed in Km/s.</p> <p> - LOCAL TRUE SOLAR TIME - Angle between the extension of the vector from the Sun to Mars and the projection on Mars' ecliptic plane of a vector from the center of the target body and the point on the target body surface that lies directly beneath the Mars Express spacecraft at the time corresponding to EPHEMERIS TIME, expressed on a 24-hour clock with decimal fractions of the hour.</p> <p>* orbit_XXXXX_frequency_Y_MHz.png, where XXXXX is the orbit number, and Y the frequency at which the radar was operating, in MHz. The file is a visualization of the corresponding radargram, of the spacecraft ground track during the observation, and of surface and subsurface echo power.</p>
Text-fig. 39. Scanning electron microscope (SEM) images of "Pollen clump with tricolpate pollen sp. 3"; Catefica locality, Portugal. a) Pollen clump, probably an anther fragment, containing one kind of pollen; b, d, e) Pollen grains from stamen fragment in polar (b, d) and equatorial (e) views showing the long colpi with coarsely verrucate aperture membranes; note the semitectate-reticulate tectum in the mesocolpium regions and foveolate-punctate tectum in the polar regions and along the aperture margins; c) Detail of pollen wall showing smooth muri and short, densely-spaced columellae. Specimen, Catefica 49-S107785 (a–e). Scale bars = 600 Μm (a), 6 Μm (b, d, e), 1.5 Μm (c). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 39. Scanning electron microscope (SEM) images of "Pollen clump with tricolpate pollen sp. 3"; Catefica locality, Portugal. a) Pollen clump, probably an anther fragment, containing one kind of pollen; b, d, e) Pollen grains from stamen fragment in polar (b, d) and equatorial (e) views showing the long colpi with coarsely verrucate aperture membranes; note the semitectate-reticulate tectum in the mesocolpium regions and foveolate-punctate tectum in the polar regions and along the aperture margins; c) Detail of pollen wall showing smooth muri and short, densely-spaced columellae. Specimen, Catefica 49-S107785 (a–e). Scale bars = 600 Μm (a), 6 Μm (b, d, e), 1.5 Μm (c).
Text-fig. 36. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–e) images of "Paisia-like follicle"; Catefica locality, Portugal. a, b) Volume rendering of follicle in lateral (a) and ventral (b) views showing the decurrent stigmatic region that extends from the follicle base to the apex but lacks a distinct papillate zone; c) Longitudinal section (volume rendering cut at orthoslice yz0341) of follicle showing under-developed ovules towards the base and numerous well-developed ovules/seeds in the upper part suggesting that the follicle is probably mature; d) Dorsal view of follicle apex showing the cleft in the presumed stigmatic apical region; e) Transverse section (orthoslice xy1294) of follicle with one dorsal and two ventral bundles and two placentae bulging into the locule, one on either side of the ventral suture; note the strongly compressed outer epidermis and the homogenized cells of the mesocarp. Specimen, Catefica 49-S174915 (a–e). Scale bars = 300 Μm (a–d), 100 Μm (e). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 36. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–e) images of "Paisia-like follicle"; Catefica locality, Portugal. a, b) Volume rendering of follicle in lateral (a) and ventral (b) views showing the decurrent stigmatic region that extends from the follicle base to the apex but lacks a distinct papillate zone; c) Longitudinal section (volume rendering cut at orthoslice yz0341) of follicle showing under-developed ovules towards the base and numerous well-developed ovules/seeds in the upper part suggesting that the follicle is probably mature; d) Dorsal view of follicle apex showing the cleft in the presumed stigmatic apical region; e) Transverse section (orthoslice xy1294) of follicle with one dorsal and two ventral bundles and two placentae bulging into the locule, one on either side of the ventral suture; note the strongly compressed outer epidermis and the homogenized cells of the mesocarp. Specimen, Catefica 49-S174915 (a–e). Scale bars = 300 Μm (a–d), 100 Μm (e).
Text-fig. 35. Scanning electron microscope (SEM, a, b, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM, c, e) images of "Paisia-like follicle"; Catefica locality, Portugal. a) Lateral view of slender follicle with an almost straight ventral margin and a slightly curved dorsal margin; b) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; c) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; d) Apical part of follicle in (a) showing the slight apical cleft in the probable stigmatic region; e) Transverse section (orthoslice xy0407) of follicle showing ovules and distinct follicle wall with small, thin-walled cells of the outer epidermis (arrow), larger, isodiametric cells of the mesocarp and an inner layer of smaller, thin-walled cells. Specimens, Catefica 50-S171523 (a, d), Catefica 343-S171515 (b), Catefica 49-S174929 (c, e). Scale bars = 300 Μm (a–c), 100 Μm (d, e). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 35. Scanning electron microscope (SEM, a, b, d) and synchrotron radiation X-ray tomographic microscopy (SRXTM, c, e) images of "Paisia-like follicle"; Catefica locality, Portugal. a) Lateral view of slender follicle with an almost straight ventral margin and a slightly curved dorsal margin; b) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; c) Lateral view of small, broad follicle with slightly sinuous ventral margin and rounded dorsal margin; d) Apical part of follicle in (a) showing the slight apical cleft in the probable stigmatic region; e) Transverse section (orthoslice xy0407) of follicle showing ovules and distinct follicle wall with small, thin-walled cells of the outer epidermis (arrow), larger, isodiametric cells of the mesocarp and an inner layer of smaller, thin-walled cells. Specimens, Catefica 50-S171523 (a, d), Catefica 343-S171515 (b), Catefica 49-S174929 (c, e). Scale bars = 300 Μm (a–c), 100 Μm (d, e).
Text-fig. 34. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–h) images of "Paisia-like follicle"; Catefica locality, Portugal. a–d) Volume rendering of follicles in lateral (a, c) and ventral (b, d) views showing the decurrent stigmatic region that extends from base of the follicle to the apex; note papillate zone forming a probable stigma along the full length of the ventral suture; e) Longitudinal section (volume rendering cut at orthoslice yz0326) near the base of the follicle showing two ovules with a striate-reticulate surface (asterisks); note transverse fibers lining the inner follicle wall and large cells of the mesocarp; f) Transverse section (volume rendering cut at orthoslice xy2475) of follicle showing two rows of ovules borne on placentae on either side of the ventral suture (asterisks); note also the pronounced and densely-spaced papillae around the ventral suture; g) Transverse section (orthoslice xy1988) of follicle showing two ventral vascular bundles and one dorsal bundle (arrows) and ovules/seeds borne on two placentae, one on either side of the ventral suture; note the remains of the small thin-walled cells of the outer epidermis that cover the thicker-walled cells of the mesocarp; h) Transverse section (orthoslice xy2860) of follicle showing two ventral bundles and one dorsal bundle (arrows) and ovules/seeds in two rows on the placentae, one on either side of the ventral suture; note the remains of small epidermal cells and the large rounded cells of the mesocarp with thicker walls. Specimens, Catefica 49-S174916 (a, b), Catefica 49-S174917 (c–f, h), Catefica 50-S171525 (g). Scale bars = 300 Μm (a–d), 100 Μm (e–h). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 34. Synchrotron radiation X-ray tomographic microscopy (SRXTM, a–h) images of "Paisia-like follicle"; Catefica locality, Portugal. a–d) Volume rendering of follicles in lateral (a, c) and ventral (b, d) views showing the decurrent stigmatic region that extends from base of the follicle to the apex; note papillate zone forming a probable stigma along the full length of the ventral suture; e) Longitudinal section (volume rendering cut at orthoslice yz0326) near the base of the follicle showing two ovules with a striate-reticulate surface (asterisks); note transverse fibers lining the inner follicle wall and large cells of the mesocarp; f) Transverse section (volume rendering cut at orthoslice xy2475) of follicle showing two rows of ovules borne on placentae on either side of the ventral suture (asterisks); note also the pronounced and densely-spaced papillae around the ventral suture; g) Transverse section (orthoslice xy1988) of follicle showing two ventral vascular bundles and one dorsal bundle (arrows) and ovules/seeds borne on two placentae, one on either side of the ventral suture; note the remains of the small thin-walled cells of the outer epidermis that cover the thicker-walled cells of the mesocarp; h) Transverse section (orthoslice xy2860) of follicle showing two ventral bundles and one dorsal bundle (arrows) and ovules/seeds in two rows on the placentae, one on either side of the ventral suture; note the remains of small epidermal cells and the large rounded cells of the mesocarp with thicker walls. Specimens, Catefica 49-S174916 (a, b), Catefica 49-S174917 (c–f, h), Catefica 50-S171525 (g). Scale bars = 300 Μm (a–d), 100 Μm (e–h).
Text-fig. 16. Scanning electron microscope (SEM) images of "Stamen fragments with in situ Clavatipollenites- or Asteropollis-type pollen" (sp. 1: a–c; sp. 2: d–f; sp. 3: g–i); Catefica locality, Portugal. a) Stamen fragment showing pollen sacs; b) Distal view of pollen grain from (a) showing semitectate-reticulate tectum; c) Detail of pollen wall showing the semitectate-reticulate tectum and long, scattered, columellae supporting muri with fine pits and rounded supratectal ornamentation; note orbiculae with a finely spiny surface (arrows); d) Stamen showing very short filament, lateral pollen sacs and short apical extension of the narrow connective; e) Folded pollen grain from (d) showing semitectate-reticulate tectum; f) Detail of pollen wall from (d) showing the semitectatereticulate tectum and muri with fine rounded ornamentation; g) Stamen fragment; h, i) Detail of pollen grains from (g) showing the semitectate-reticulate tectum with smooth muri, long scattered columellae and tiny scattered orbicules (arrow). Specimens, Catefica 50-S170395 (a–c), Catefica 49-S172561 (d–f), Catefica 50-S170390 (g–i). Scale bars = 600 Μm (a, d, g), 6 Μm (b, e, h), 1.5 Μm (c, f, i). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 16. Scanning electron microscope (SEM) images of "Stamen fragments with in situ Clavatipollenites- or Asteropollis-type pollen" (sp. 1: a–c; sp. 2: d–f; sp. 3: g–i); Catefica locality, Portugal. a) Stamen fragment showing pollen sacs; b) Distal view of pollen grain from (a) showing semitectate-reticulate tectum; c) Detail of pollen wall showing the semitectate-reticulate tectum and long, scattered, columellae supporting muri with fine pits and rounded supratectal ornamentation; note orbiculae with a finely spiny surface (arrows); d) Stamen showing very short filament, lateral pollen sacs and short apical extension of the narrow connective; e) Folded pollen grain from (d) showing semitectate-reticulate tectum; f) Detail of pollen wall from (d) showing the semitectatereticulate tectum and muri with fine rounded ornamentation; g) Stamen fragment; h, i) Detail of pollen grains from (g) showing the semitectate-reticulate tectum with smooth muri, long scattered columellae and tiny scattered orbicules (arrow). Specimens, Catefica 50-S170395 (a–c), Catefica 49-S172561 (d–f), Catefica 50-S170390 (g–i). Scale bars = 600 Μm (a, d, g), 6 Μm (b, e, h), 1.5 Μm (c, f, i).
Text-fig. 4. Synchrotron radiation X-ray tomographic microscopy (SRXTM) images of seeds of Canrightia foveolata sp. nov.; Catefica locality, Portugal. a–d) Volume renderings of abraded seeds in ventral (a), lateral (b, d) and apical (c) views showing the slightly protruding chalaza (arrows) and dense longitudinal grooves with shallow pits in the surface of the endotesta; e) Transverse section of seed (orthoslice xy0665) showing the irregular grooved surface of the endotesta (oi-en) and the tegmen comprised of two layers of thick-walled cells that surround the cells of the prominent endothelium (asterisk); f) Longitudinal section (orthoslice xz1195) through seed showing the thin-walled endothelium cells (asterisk) surrounded by the thicker cells of the outer tegmen and endotesta; g) Longitudinal section (orthoslice yz0727) through seed showing outlines of angular crystals evenly distributed in cells of the endotesta (oi-en); note the outer epidermis of the tegmen (ii-o) composed of thick-walled cells; h) Longitudinal section (orthoslice xz0940) of seed showing details of the chalazal region with course of the vascular bundle (vb), cells of the prominent endothelium (asterisk), crystalliferous endotesta of the outer integument (oi-en) and the distinct thick walled cells of the outer cells of the tegmen (ii-o); i) Longitudinal and tangential section (orthoslice yz0542) through the endotesta (oi-en) showing the outlines of densely spaced crystals. Specimens, Catefica 242-S175178 (a–c, e–h), Catefica 49-S175179 (d, i). Scale bars = 300 Μm (a–d), 100 Μm (e, f, h, i), 50 Μm (g). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 4. Synchrotron radiation X-ray tomographic microscopy (SRXTM) images of seeds of Canrightia foveolata sp. nov.; Catefica locality, Portugal. a–d) Volume renderings of abraded seeds in ventral (a), lateral (b, d) and apical (c) views showing the slightly protruding chalaza (arrows) and dense longitudinal grooves with shallow pits in the surface of the endotesta; e) Transverse section of seed (orthoslice xy0665) showing the irregular grooved surface of the endotesta (oi-en) and the tegmen comprised of two layers of thick-walled cells that surround the cells of the prominent endothelium (asterisk); f) Longitudinal section (orthoslice xz1195) through seed showing the thin-walled endothelium cells (asterisk) surrounded by the thicker cells of the outer tegmen and endotesta; g) Longitudinal section (orthoslice yz0727) through seed showing outlines of angular crystals evenly distributed in cells of the endotesta (oi-en); note the outer epidermis of the tegmen (ii-o) composed of thick-walled cells; h) Longitudinal section (orthoslice xz0940) of seed showing details of the chalazal region with course of the vascular bundle (vb), cells of the prominent endothelium (asterisk), crystalliferous endotesta of the outer integument (oi-en) and the distinct thick walled cells of the outer cells of the tegmen (ii-o); i) Longitudinal and tangential section (orthoslice yz0542) through the endotesta (oi-en) showing the outlines of densely spaced crystals. Specimens, Catefica 242-S175178 (a–c, e–h), Catefica 49-S175179 (d, i). Scale bars = 300 Μm (a–d), 100 Μm (e, f, h, i), 50 Μm (g).
Text-fig. 15. Scanning electron microscope (SEM) images of stamen with "Asteropollis-type pollen sp. 2"; Catefica locality, Portugal. a) Stamen with pollen in situ showing the dome-shaped extension of the connective with hairs; b–d) Pollen in situ in stamen in (a) showing poorly defined pentachotomocolpate (b, d) and possibly tetrachotomocolpate (c) apertures and semitectate-reticulate tectum; e) Detail of broken pollen grains showing the loosely attached reticulum, long scattered columellae and thick foot layer. Specimen, Catefica 49-S101209 (a–e). Scale bars = 600 Μm (a), 6 Μm (b–e). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 15. Scanning electron microscope (SEM) images of stamen with "Asteropollis-type pollen sp. 2"; Catefica locality, Portugal. a) Stamen with pollen in situ showing the dome-shaped extension of the connective with hairs; b–d) Pollen in situ in stamen in (a) showing poorly defined pentachotomocolpate (b, d) and possibly tetrachotomocolpate (c) apertures and semitectate-reticulate tectum; e) Detail of broken pollen grains showing the loosely attached reticulum, long scattered columellae and thick foot layer. Specimen, Catefica 49-S101209 (a–e). Scale bars = 600 Μm (a), 6 Μm (b–e).
Text-fig. 23. Synchrotron radiation X-ray tomographic microscopy (SRXTM) images of Ibericarpus cuneiformis gen. et sp. nov.; Catefica locality, Portugal. a) Longitudinal section (orthoslice yz0827) showing fruitlet with apical stigmatic region and a single seed enclosed within the fruitlet wall that has a thick epidermal cuticle; note the tiny embryo (emb) internal to the micropyle (mi) and oriented toward the base of the fruitlet; b) Transverse section through apical part of two fruitlets (orthoslice xy0810) showing the fruitlet wall (fr) composed of small thin-walled cells covered by an epidermis of isodiametric cells (ep) with a thick outer cuticle (cu); c) Longitudinal section (orthoslice xz0370) through basal part of fruitlet perpendicular to section in (a) showing the micropyle (mi), embryo (emb) composed of tiny cells, and the thick cuticle (cu) covering the bulging cells of the fruitlet epidermis. Specimen, Catefica 50-S174907 (a–c). Scale bars = 300 Μm (a–c). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 23. Synchrotron radiation X-ray tomographic microscopy (SRXTM) images of Ibericarpus cuneiformis gen. et sp. nov.; Catefica locality, Portugal. a) Longitudinal section (orthoslice yz0827) showing fruitlet with apical stigmatic region and a single seed enclosed within the fruitlet wall that has a thick epidermal cuticle; note the tiny embryo (emb) internal to the micropyle (mi) and oriented toward the base of the fruitlet; b) Transverse section through apical part of two fruitlets (orthoslice xy0810) showing the fruitlet wall (fr) composed of small thin-walled cells covered by an epidermis of isodiametric cells (ep) with a thick outer cuticle (cu); c) Longitudinal section (orthoslice xz0370) through basal part of fruitlet perpendicular to section in (a) showing the micropyle (mi), embryo (emb) composed of tiny cells, and the thick cuticle (cu) covering the bulging cells of the fruitlet epidermis. Specimen, Catefica 50-S174907 (a–c). Scale bars = 300 Μm (a–c).
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.