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Supplementary material 2 from: Costa AF, Siciliano S, Emin-Lima R, Martins BML, Sousa MEM, Giarrizzo T, Silva Júnior JS (2017) Stranding survey as a framework to investigate rare cetacean records of the north and north-eastern Brazilian coasts. ZooKeys 688: 111-134. https://doi.org/10.3897/zookeys.688.12636

S2 Movie : Explanation note: Rescue Guiana dolphins trapped at Praia da Corvina, Salinópolis (MOV).

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Figure 5 from: Costa AF, Siciliano S, Emin-Lima R, Martins BML, Sousa MEM, Giarrizzo T, Silva Júnior JS (2017) Stranding survey as a framework to investigate rare cetacean records of the north and north-eastern Brazilian coasts. ZooKeys 688: 111-134. https://doi.org/10.3897/zookeys.688.12636

Figure 5 - Cluster analysis of cetacean stranding and sighting records. Diagram of cetaceans reported from the north-eastern (inverted triangle) and north coast of Brazil (triangle) and southern Caribbean (square), reported in seven articles and the present study. The two distinct groups formed (Group 1 and 2) are indicated. A similarity profile (SIMPROF) permutation test highlights dashed clusters that show significant internal structure. Shaded cells indicate occurrence of species.

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Figure 4 from: Costa AF, Siciliano S, Emin-Lima R, Martins BML, Sousa MEM, Giarrizzo T, Silva Júnior JS (2017) Stranding survey as a framework to investigate rare cetacean records of the north and north-eastern Brazilian coasts. ZooKeys 688: 111-134. https://doi.org/10.3897/zookeys.688.12636

Figure 4 - Stranding events: small and medium-sized cetaceans. a The carcass of a Fraser's dolphin, Lagenodelphis hosei was cut down on the village of Carnaubeira, Araioses municipality (2°42.6'S, 42°0.6'W) and consumed locally (photo PROCEMA files) b A group of 12 live Guiana dolphins Sotalia guianensis were found trapped (photo showed just one specimen) in a tidal channel alongside Corvina beach, Salinópolis municipality, eastern Pará (0°36'S, 47°22.2'W) c A False killer whale Pseudorca crassidens stranded on the north-eastern coast of Pará, Praia de Marudá, Marapanim municipality (photo GEMAM files) d A Short-finned pilot whale Globicephala macrorhynchus stranded in advanced stage of decomposition at Praia da Pedra do Sal, Parnaíba municipality (2°40.8'S, 42°31.2'W), the shape of the head and number of teeth were diagnostic to identify the species (photo PROCEMA files).

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Figure 2 from: Costa AF, Siciliano S, Emin-Lima R, Martins BML, Sousa MEM, Giarrizzo T, Silva Júnior JS (2017) Stranding survey as a framework to investigate rare cetacean records of the north and north-eastern Brazilian coasts. ZooKeys 688: 111-134. https://doi.org/10.3897/zookeys.688.12636

Figure 2 - Stranding events: large baleen whales. a Fin whale Balaenoptera physalus stranded at Baía do Japerica, São João de Pirabas municipality (0°45'S, 47°4.2'W), eastern coast of the Pará state. Note the good condition of the carcass and the strange swelling on the top of the head (see white arrow) (photo by GEMAM/MPEG) b A newborn Bryde's whale Balaenoptera brydei stranded on the east coast of Marajó island (0°51.6'S, 48°30'W), Pará on 15 September 2012 (photo by GEMAM/MPEG) c Humpback whale Megaptera novaeangliae stranded at Praia do Rio Novo, Tutóia municipality, Maranhão state (2°42.6'S, 42°26.4'W), note the absence of skull on the carcass (photo by A.F. Costa) d Sei whale Balaenoptera borealis stranded at Fernandes Belo, Viseu municipality (1°10.8'S, 46°5.4'W), the particular coloration of the baleen plates and slightly arched head are diagnostic characters to identify the species (photo by DEMA/PA).

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Figure 3 from: Costa AF, Siciliano S, Emin-Lima R, Martins BML, Sousa MEM, Giarrizzo T, Silva Júnior JS (2017) Stranding survey as a framework to investigate rare cetacean records of the north and north-eastern Brazilian coasts. ZooKeys 688: 111-134. https://doi.org/10.3897/zookeys.688.12636

Figure 3 - Stranding events: sperm whales. a Sperm whale Physeter macrocephalus (MPEG 42166) stranded at Praia do Crispim, Marapanim municipality (0°34.8'S, 47°38.4'W) eastern coast of Pará state few hours after death (photo by A.F. Costa) b The same specimen (MPEG 42166) still alive with local people trying to help (internet file) c A newborn Sperm whale (MPEG 42178) stranded at the east coast of Marajó island, Praia do Camburupy (0°32.4'S, 48°28.2'W) on 2014, note the absence of teeth characteristic of a very young specimen (photo by A.F. Costa) d A female Sperm whale (MPEG 42088) stranded at Praia da Pedra do Sal, Parnaíba municipality, Piauí state coastline (2°49.8'S, 41°41.4'W) on 2010 (photo by A.F. Costa).

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Figure 1 from: Costa AF, Siciliano S, Emin-Lima R, Martins BML, Sousa MEM, Giarrizzo T, Silva Júnior JS (2017) Stranding survey as a framework to investigate rare cetacean records of the north and north-eastern Brazilian coasts. ZooKeys 688: 111-134. https://doi.org/10.3897/zookeys.688.12636

Figure 1 - Study sites. Surveyed sectors on north and north-eastern Brazilian coast: 1 Marajó Bay 2 Eastern Pará state and 3 Maranhão and Piauí coastlines encompass part of Parnaíba Delta. Black dots representing stranding locations. Physeter macrocephalus (Pma), Balaenoptera brydei (Bbr), Balaenoptera physalus (Bph), Megaptera novaeangliae (Mno), Steno bredanensis (Sbr), Balaenoptera borealis (Bbo), Tursiops truncatus (Ttr), Globicephala macrorhynchus (Gma), Pseudorca crassidens (Pcr), Sotalia guianensis (Sgu), Peponocephala electra (Pel), Stenella attenuata (Sat), Lagenodelphis hosei (Lho), Delphinus sp. (Dsp) and Grampus griseus (Ggr).

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Fig. 5 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)

Fig. 5. Relative expressional levels of HSP90 mRNA in P. citri adult females afer treatment at 32 °C with abamectin concentrations of LC25, LC50 and LC75 for the indicated times. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with abamectin and control group (t-test, P <0.05).

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Fig. 3 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)

Fig. 3. Relative expressional levels of HSP90 mRNA in P. citri adult females afer treatment with abamectin at LC25, LC50 and LC75levels for the indicated times at 18 °C.Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with abamectin and control group (t-test, P <0.05).

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Fig. 1 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)

Fig. 1. The cDNA and deduced amino acid sequence of HSP90 of Panonychus citri adult females. The start and stop codons are boxed; the numbers on the lef are for the positions of nucleotides and amino acids in the sequences.

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Fig. 7 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)

Fig. 7. Relative expressional levels of HSP90 mRNA in adult female P. citri Mc- Gregor afer treatment with dsRNA at concentrations equivalent to 0.1 µg/µL, 0.2 µg/µL and 0.4 µg/µL. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with dsRNA and control group (t-test, P <0.05).

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Fig. 4 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)

Fig. 4. Relative expressional levels of HSP90 mRNA in P. citri adult females afer treatment at 25 °C with abamectin concentrations of LC25, LC50 and LC75 for the indicated times. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with abamectin and control group (t-test, P <0.05).

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Fig. 6 in Molecular cloning of heat shock protein gene HSP90 and effects of abamectin and double-stranded RNA on its expression in Panonychus citri (Trombidiformes: Tetranychidae)

Fig. 6. Relative expressional levels of HSP90 mRNA in P. citri adult females subjected to 18 °C or 32 °C for the length of time indicated. Data are shown as means of 3 replicates ± SD. The relative expression level of HSP90 mRNA in the control group is equal to 1.0 and is marked with a dashed line. The asterisk above the bar indicates a significance difference between treatment with temperature and control group (t-test, P <0.05).

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Figure 7 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 7 Cibarium of Dudaica species (part 2). Aqiongzhouensis sp. n. (paratype #03313) Borthophallata sp. n. (paratype #03905) Cdissimilis (holotype #00430). Scale bars: 0.1 mm.

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Figure 8 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 8 Prementum (ventral and lateral views). Amalayana (Takada) (#03903) Bgracilipalpis sp. n. (paratype #00492) Cpuberula sp. n. (paratype #03366) Dalbipalpis sp. n. (holotype #03908) Eqiongzhouensis sp. n. (paratype #03313) Forthophallata sp. n. (paratype #03905) Gdissimilis sp. n. (holotype #00430). Scale bars: 0.1 mm.

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Figure 11 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 11 Drosophila (Dudaica) gracilipalpis sp. n. (A, M–O paratype #00492 B–K paratype #03902 L paratype #00491). A tibia of right foreleg (apical seta indicated with red arrowhead; ventral view) B, C periphallic organs (caudal and caudolateral view, respectively) D surstyli (caudoventral view) E surstylus and epandrial ventral lobe (caudolateral view) F tenth sternite and ventral protrusions of cerci (ventral view) G–I phallic organs (ventral, ventrolateral, and lateral view, respectively) J pubescence on caudolateral lobe of hypandrium (indicated with red arrowhead; caudoventral view) K, L sensilla (indicated with red arrowheads) and apical portion of parameres (ventral view; Indonesian and Chinese specimen, respectively) M, N oviscapt (lateral and ventral view, respectively) O spermatheca. Scale bars: 0.1 mm.

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Figure 4 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 4 Head (anterior view). Amalayana (Takada) (#03903) Bgracilipalpis sp. n. (paratype #00492) Cpuberula sp. n. (paratype #03366) Dalbipalpis sp. n. (holotype #03908) Eqiongzhouensis sp. n. (paratype #03313) Forthophallata sp. n. (paratype #03905) Gdissimilis sp. n. (holotype #00430). Abbreviation: plp = palpus. Scale bars: 0.5 mm.

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Figure 14 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 14 Drosophila (Dudaica) qiongzhouensis sp. n. (A–K paratype #03418 L–N paratype #03313). A, B periphallic organs (caudal and caudolateral view, respectively) C surstyli (caudoventral view) D surstylus and epandrial ventral lobe (caudolateral view) E tenth sternite and ventral protrusions of cerci (ventral view) F–H phallic organs (ventral, ventrolateral, and lateral view, respectively) I apical portion of aedeagus with fine serrations (indicated with red arrowhead; ventrolateral view) J, K sensilla (indicated with red arrowheads) and/or apical portion of parameres (ventral and lateral view, respectively) L, M oviscapt (lateral and ventral view, respectively) N spermatheca. Scale bars: 0.1 mm.

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Figure 10 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 10 Drosophila (Dudaica) malayana (Takada) (A, K #03903 B–J #03904 L–N female specimen). A tibia of right foreleg (apical setae indicated with red arrowheads; ventral view) B, C periphallic organs (caudal and caudolateral view, respectively) D surstyli (caudoventral view) E surstylus and epandrial ventral lobe (caudolateral view) F tenth sternite and ventral protrusions of cerci (ventral view) G–I phallic organs (ventral, ventrolateral, and lateral view, respectively) J pubescence on caudolateral lobe of hypandrium (indicated with red arrowhead; caudoventral view) K sensilla (indicated with red arrowheads) and apical portion of parameres (ventral view) L, M oviscapt (lateral and ventral view, respectively) N spermatheca. Abbreviations: aed = aedeagus, aed a = aedeagal apodeme, cerc = cercus, epand = epandrium, hypd = hypandrium, pm = paramere, sur = surstylus. Scale bars: 0.1 mm.

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Figure 15 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 15 Drosophila (Dudaica) orthophallata sp. n. (A–H holotype #00177 I, J paratype #03906). A, B periphallic organs (caudal and caudolateral view, respectively) C surstyli, tenth sternite, and ventral protrusions of cerci (ventral view) D epandrial ventral lobe (caudolateral view) E–G phallic organs (ventral, ventrolateral, and lateral view, respectively) H sensilla (indicated with red arrowheads) and apical portion of parameres (ventral view) I, J oviscapt (lateral and ventral view, respectively). Scale bars: 0.1 mm.

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Figure 6 from: Katoh TK, Zhang G, Toda MJ, Suwito A, Gao J-J (2018) A revision of the subgenus Dudaica Strand of the genus Drosophila Fallén, with descriptions of six new species (Diptera, Drosophilidae). ZooKeys 781: 19-50. https://doi.org/10.3897/zookeys.779.27354

Figure 6 Cibarium of Dudaica species (part 1; dorsal and lateral views). Amalayana (Takada) (#03903) Bgracilipalpis sp. n. (paratype #00492) Cpuberula sp. n. (paratype #03366) Dalbipalpis sp. n. (holotype #03908). Abbreviations: cly = clypeus, m s = medial sensilla, p s = posterior sensilla. Scale bars: 0.1 mm.

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