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opencc-zeroJan 2024View details →

Polystyrene-induced changes in adipose transcriptome

Microplastics (MP) derived from the weathering of polymers, or synthesized in this size range, have become widespread environmental contaminants and have found their way into water supplies and the food chain. Despite this awareness, little is known about the health consequences of MP ingestion. We have previously shown that the consumption of polystyrene (PS) beads was associated with intestinal dysbiosis, diabetes, and obesity in mice. To further evaluate the systemic metabolic effects of PS on the gut-liver-adipose tissue axis, we supplied C57BL/6J mice with normal water or that containing two sizes of PS beads (0.5mm and 5mm) at a concentration of 1mg/ml. After 13wk, we evaluated several indices of metabolism and liver function. As previously observed, mice drinking the PS-containing water had a potentiated weight gain and adipose expansion. Here we found that this was associated with an increased abundance of adipose F4/80+ macrophages. These exposures did not cause non-alcoholic fatty liver disease (NAFLD) but were associated with decreased liver:body weight ratios and an enrichment in hepatic FXR and LXR signaling. PS also increased hepatic cholesterol and altered both hepatic and cecal bile acids. Mice consuming PS beads and treated with the berry anthrocyanin, delphinidin, demonstrated an attenuated weight gain compared to those mice receiving a control intervention and also exhibited a down-regulation of cAMP and PPAR signaling pathways. This study highlights the obesogenic role of PS in perturbing the gut-liver-adipose axis and altering nuclear receptor signaling and intermediary metabolism. Dietary interventions may limit the adverse metabolic effects of PS consumption.

restrictedcc-by-4.0Nov 2024View details →

The dorsal aortic compartment is a developmental source of brown adipose tissue in mice

We FACS-sorted Osr1-expressing cells (Osr1 GCE/+) from 14 E9.5 and 6 E11.5 mouse embryos and analyzed their gene expression profiles using single-cell RNA sequencing. The data was processed and integrated using Seurat v3. The sample and annotation informations can be accessed via the "time" and "annotations" columns respectively.

opencc-zeroNov 2021View details →

Posterior cervical brown fat and CXCL14 levels in the first year of life: sex differences and association with adiposity

Context: Brown adipose tissue (BAT) is particularly abundant in neonates but its association with measures of adiposity and metabolic health in early infancy is poorly delineated. Besides sustaining non-shivering thermogenesis, BAT secretes brown adipokines that act on systemic metabolism. The chemokine CXCL14 has been identified as a brown adipokine in experimental studies. Objective: To determine the relationships among BAT activity, adiposity and circulating CXCL14 levels in the first year of life in girls and boys. Design, setting and participants: Indices of fat accretion, circulating endocrine-metabolic parameters and serum CXCL14 levels were assessed longitudinally in a cohort of infants at birth and at 4 and 12 months. BAT activity was estimated using infrared thermography only at age 12 months. Main outcome measures: Weight and length Z-scores, total and abdominal fat content (by DXA), BAT activity at the posterior-cervical and supraclavicular regions, serum levels of glucose, insulin, insulin-like growth factor-I, high-molecular-weight adiponectin and CXCL14; CXCL14 transcript levels in neonatal BAT and liver. Results: Posterior-cervical BAT was more active in girls than in boys (p=0.02). BAT activity was negatively associated with adiposity parameters only in girls. CXCL14 levels were higher in girls than in boys at age 12 months and correlated positively with the area of active posterior-cervical BAT in girls. Neonatal BAT showed high CXCL14 gene expression levels. Conclusions: BAT activity and the levels of CXCL14 -a potential surrogate of BAT activity- are sex-specific in the first year of life. BAT activity associates negatively with indices of adiposity only in girls.

opencc-by-4.0Mar 2022View details →

Differences between four skinfold calipers in the assessment of adipose tissue in a healthy young adult population

Database of the paper: Differences between four skinfold calipers in the assessment of adipose tissue in a healthy young adult population

opencc-by-4.0Apr 2022View details →

Interrelationship among thigh intermuscular adipose tissue, cross-sectional area, muscle strength and functional mobility in older subjects

Objectives: The aim of this cross-sectional study was to investigate the association between lower limb strength, muscle mass and composition and balance ability in elders. Methods: Thirthy-four older participants (Age: 65.6±4.73 years; male=10 and female=24) were assessed for muscle strength (maximal isometric strength of knee extensors and one repetition maximum by leg press, 1RM), balance and gait capacity (Mini-BESTest), body composition by DXA (obtaining Appendicular Skeletal Muscle Mass Index, ASMMI) and magnetic resonance imaging of thigh to evaluate Intermuscular Adipose Tissue (IMAT) and muscle Cross Sectional Area (CSA). Results: Positive correlations between 1RM leg press and ASMMI (rs=0.64, P<.0001) and thigh CSA (rs=0.52, P=.0017), but not with thigh IMAT, were found. In addition, significant correlations between knee extensors strength and ASMMI (rs=0.48, P=.004) and thigh CSA (rs=0.49, P=.0033) and IMAT (rs=-0.35, P=.043) were observed whereas no significant correlations between the Mini-BESTest with ASMMI, thigh CSA and IMAT were observed. Discussion: Lower limb strength positively correlated with appendicular muscle mass. Further, the maximal isometric strength of knee extensors negatively correlated with thigh IMAT in elderly patients whereas the dynamic balance ability did not correlate with any of the morphological variables of the muscle (i.e. ASMMI, CSA and IMAT). A reduced muscle size and strength could affect movement and reduce physical function in older patients. Improving the composition and size of muscle in elder subjects could reduce frailty and risk of falls.

opencc-zeroDec 2017View details →

Data from: Validity of bioelectrical impedance analysis in predicting total body water and adiposity among Senegalese school-aged children

Introduction: Childhood obesity is currently a serious public health challenge in developing countries. Therefore, an accurate assessment of adiposity is required. The objective of this study was to validate BIA prediction equations for the assessment of total body water and adiposity or percentage of body fat for the first time in Senegalese school-aged children. Methods: One-hundred-fifty-one (151) pupils who were 8-11 years old were randomly selected from four public schools in Dakar. The body composition measured by deuterium dilution method (DDM) was used as the reference method and compared to that predicted by BIA using a multi-frequency analyser. Stepwise backward multiple linear regression was performed to calculate TBW and %BF in a subsample, which were then validated it in the rest of the sample. The Bland and Altman approach was used to assess the agreement between the two methods (bias and limits of agreement). Results: FFM was higher in boys (24.6±6.9 kg) compared to girls (21.2±3.3 kg; P<0.001), and %BF was lower in boys (5.1±4.6 kg) compared to girls (5.3±3.8 kg; P<0.05). Overall, 11.3% of children were obese (%BF ≥25% in boys, and ≥30% in girls) and 4.6% were overweight/obese according to their BMI-z score (>+1). The equations developed were as follows: TBW=0.376(Height²/Z50)-0.470 (sex) +0.076(weight) +0.065(height)-2.28. %BF= -1.10(height²/Z50) +3.14(sex)+1.57(weight)-4.347. These specific equations showed good precision and a low and non-significant mean bias (0.11 kg, P=0.279; and 0.19 kg, P=0.764) for TBW and %BF, respectively. Conclusion: The newly developed equations can be used as an accurate and alternative tool for screening for obesity among African school-aged children in various settings

opencc-zeroOct 2022View details →

Correlation analysis of epicardial adipose tissue and ventricular myocardial strain in Chinese amateur marathoners using cardiac magnetic resonance

The volume of epicardial adipose tissue (EAT) is associated with an increased incidence of cardiovascular disease (CVD); however, only a few studies have examined its effect on the myocardial function of endurance in athletes. The association between the EAT and the variation of myocardial function is still unclear in amateur marathoners. Consequently, by using some sedentary individuals as the control, this study aims to evaluate the correlation between the EAT volume and the myocardial strain in the left and right ventricles of Chinese amateur marathoners by cardiac magnetic resonance (CMR).The volume of epicardial adipose tissue (EAT) is associated with an increased incidence of cardiovascular disease (CVD); however, only a few studies have examined its effect on the myocardial function of endurance in athletes. The association between the EAT and the variation of myocardial function is still unclear in amateur marathoners. Consequently, by using some sedentary individuals as the control, this study aims to evaluate the correlation between the EAT volume and the myocardial strain in the left and right ventricles of Chinese amateur marathoners by cardiac magnetic resonance (CMR).

opencc-by-4.0Jun 2024View details →

Cartilage Responses to Inflammatory Stimuli and Adipose Stem/Stromal Cell-Derived Conditioned Medium: Results from an Ex Vivo Model

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FIGURE 6 in New species of Neoplecostomus (Neoplecostomini: Loricariidae) lacking adipose fin from upper Rio Paraná basin, central Brazil

FIGURE 6 Map showing the distribution of Neoplecostomus species. 1) N. watersi, type locality at Córrego Cachoeira, upper Rio Paraná basin, Goiás state, Brazil; 2) N. corumba; 3) N. franciscoensis; 4) N. doceensis; 5) N. espiritosantensis; 6) N. canastra; 7) N. langeanii; 8) N. paranensis; 9) N. paraty; 10) N. bandeirante; 11) N. ribeirensis; 12) N. jaguari; 13) N. botucatu; 14) N. selenae; 15) N. yapo; and 16) N. granosus, N. microps and N. variipictus

FIGURE 5 in New species of Neoplecostomus (Neoplecostomini: Loricariidae) lacking adipose fin from upper Rio Paraná basin, central Brazil

FIGURE 5. Picture showing the lower lip reaching or almost reaching branquial apertures ansversal line in Neoplecostomus watersi (left image), NUP 20525, 69.5 mm SL, and far from reaching branquial apertures transversal line in N. paranensis (right image), MZUSP 38572, holotype, 71.4 mm SL.

FIGURE 7 in New species of Neoplecostomus (Neoplecostomini: Loricariidae) lacking adipose fin from upper Rio Paraná basin, central Brazil

FIGURE 7. Habitat of the type locality where Neoplecostomus watersi was collected in the Córrego Cachoeira, 18°34'50.8" S 52°05'52.8" W, Goiás state, Brazil.

FIGURE 4 in New species of Neoplecostomus (Neoplecostomini: Loricariidae) lacking adipose fin from upper Rio Paraná basin, central Brazil

FIGURE 4. Neoplecostomus botucatu (a–b), paratypes, LBP 8065, 75.5–91.4 mm SL; Neoplecostomus paranensis (c–d), LBP 14631, 68.0–76.4 mm SL; right column: spine adpressed and fused to caudal peduncle; left column adipose fin absent with two or three azygous plates in the place of adipose fin.

FIGURE 2. Neoplecostomus watersi, MZUSP 123956, 69.7 in New species of Neoplecostomus (Neoplecostomini: Loricariidae) lacking adipose fin from upper Rio Paraná basin, central Brazil

FIGURE 2. Neoplecostomus watersi, MZUSP 123956, 69.7 mm SL, holotype, live specimen, from Córrego Cachoeira, Goiás state, Brazil.

FIGURE 1. Neoplecostomus watersi, MZUSP 123956 in New species of Neoplecostomus (Neoplecostomini: Loricariidae) lacking adipose fin from upper Rio Paraná basin, central Brazil

FIGURE 1. Neoplecostomus watersi, MZUSP 123956, male, 69.7 mm SL, holotype, from Goiás state, municipality of Aporé, Córrego Cachoeira, upper Rio Paraná basin.

opencc-by-4.0Apr 2019View details →

Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation

Supplemental Fig. S1 (related to Fig. 2).  Mapping and detection of sensory neurons in human pancreatic duct lesion formation. Supplemental Table S1.  Summary of color codes presented in illustrations. Supplemental Table S2.  Summary of primary and secondary antibodies used in illustrations.   Supplemental Video Legends Supplemental Video S1 (related to Fig. 2) Human pancreatic substance P+ sensory (afferent) nerves. Left (still image): projection of pancreatic innervation at the acinar-islet boundary. Blue: glucagon (islet). Green: neuroendocrine marker PGP9.5 (nerve and islet). Red: substance P (SP, sensory nerve). Sensory nerves appear as yellow fibers (overlap of red SP and green PGP9.5 signals). Broken arrow: enlarged area (right panel). Right: in-depth recording of peri-ductal sensory nerves. Sensory nerves (yellow) are around the islet but not penetrating into the core. White: nuclei. Note that a portion of islet cells are SP dim. The still image and recording were derived from a normal human pancreas (female/age, 51 years/BMI, 20).   Supplemental Video S2 (related to Fig. 2 and Supplemental Fig. S1) Mapping and detection of immunoreactive substance P+ neuron in duct lesion formation.   Left (still image): tissue map of human pancreas distal to ductal adenocarcinoma. Acinar atrophy is apparent in this condition. Markers: nuclei, white; PGP9.5, green; substance P (SP), red. Broken arrow: intra-pancreatic ganglion (right panel). Right: in-depth recording of immunoreactive SP+ neuron in ganglion. The nucleus (00:04) and the SP+ varicosities are prominently seen. The latter contacts the surrounding SP- neurons. The still image and recording were derived from an area 5-cm distal to the pancreatic ductal adenocarcinoma (male/age, 77 years/staging, T1N0).   Supplemental Video S3 (related to Fig. 3) Mouse pancreatic substance P+ sensory (afferent) nerves. Left (still image): projection of pancreatic sensory innervation. In mice, the SP+ sensory nerves follow the arteriole to the islet mantle and penetrating into the core (enlarged in right panel). Markers: blood vessels, blue; PGP9.5, green; substance P (SP), red. Right: in-depth recording of sensory innervation of islet. Unlike the human pancreatic sensory innervation, the mouse SP+ sensory nerves (varicosities) are inside the islet and associate with the peri-islet ganglion. White: nuclear staining and tissue autofluorescence. The still image and recording were derived from a B6 mouse pancreas (male/age, 12 weeks).

opencc-by-4.0Nov 2024View details →

Supplementary tables- FGF21 and its underlying adipose tissue-liver axis inform cardiometabolic burden and improvement in obesity after metabolic surgery

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opencc-by-4.0Nov 2022View details →

Dataset for puplication: Machine Learning in Automated Monitoring of Metabolic Changes Accompanying the Differentiation of Adipose Tissue-Derived Human Mesenchymal Stem cells employing 1H-1H TOCSY NMR

Data set used in the publication: Machine Learning in Automated Monitoring of Metabolic Changes Accompanying the Differentiation of Adipose Tissue-Derived Human Mesenchymal Stem cells employing 1H-1H TOCSY NMR. Abstract: In this work, the dynamic evolution of adipose tissue-derived human MSCs (AT-derived hMSCs) after fourteen days of cultivation, adiobocytes and osteocytes differentiation has been inspected based on 2D NMR TOCSY using machine learning techniques. Multi-class classification in addition to novelty detection of metabolites was established based on the profile of a control hMSCs sample at four days cultivation and successively detect the absence and the abundance of metabolites in differentiated MSCs following a set of 1H-1H TOCSY profiles. The uploaded files are: File:  metabolites_names.xlsx contain the names of the used metabolites. File: metabolites.xlsx  column 1: metabolite abbreviation column 2: 2D NMR TOCSY horizontal and vertical frequencies of metabolites in the control group at 4 days cultivation (Ct d4) column 3: 2D NMR TOCSY horizontal and vertical frequencies of metabolites found after 14 days of cultivation (Ct d14) column 4: 2D NMR TOCSY horizontal and vertical frequencies of metabolites found after 14 days of differentiation into adipocytes (AT d14) column 5: 2D NMR TOCSY horizontal and vertical frequencies of metabolites found after14 days of differentiation into osteocytes (OS d14) column 6: 2D NMR TOCSY horizontal and vertical standard frequencies of all metabolites measured at broadband high resolution 600.13 MHz NMR

opencc-by-4.0Dec 2022View details →

Supplementary information files: Gene co-expression network and differential expression analyses of subcutaneous white adipose tissue reveal novel insights into the pathological mechanisms underlying ketosis in dairy cows

Supplementary information files: Gene co-expression network and differential expression analyses of subcutaneous white adipose tissue reveal novel insights into the pathological mechanisms underlying ketosis in dairy cows

opencc-by-4.0Mar 2023View details →

Serum starvation affects mitochondrial metabolism of Adipose-derived Stem/stromal Cells

Dataset of the scientific publication 'Serum starvation affects mitochondrial metabolism of Adipose-derived Stem/stromal Cells '