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1,542 results for “Calcium”
Growing Arabidopsis pollen tubes expressing genetically encoded reporters for calcium and pH
<p>Spatiotemporal dynamics of intracellular pH (H+) and calcium (Ca2+) in growing Arabidopsis pollen tubes. Fluorescence time-lapses of pollen tubes expressing ratiometric sensors under the pollen-specific LAT52 promoter, namely CaMeleon (Ca2+) [Damineli et al., 2017; DOI: 10.1093/jxb/erx032] and pHluorin (pH) [Hoffmann et al., 2020; DOI: 10.1038/s41467-020-16253-1], were acquired every 4 seconds with an inverted microscope equipped with a front-illuminated camera (2560 × 2160, pixel size 6.45 μm), a fluorescence illuminator, and a ×63 1.2NA water immersion objective lens. The CaMeleon filters were set as follows: excitation 426-450 nm (CFP) and 505-515 nm (YFP), emission 458-487 nm (CFP) and 520-550 nm (YFP). As for pHluorin, the filter settings were: excitation 318-390 nm (DAPI) and 428-475 nm (FITC), emission 435-448 nm (DAPI) and 523-536 nm (FITC) [from Damineli et al., 2017; Hoffmann et al., 2020].</p>
Decreased FAM13B expression increases atrial fibrillation susceptibility by regulating sodium current and calcium handling
<p><strong>Objectives</strong>: To determine the causal genetic variant and gene and the mechanism for the atrial fibrillation (AF) genome wide association study (GWAS) locus on chromosome 5q31.</p> <p><strong>Background</strong>: <em>FAM13B</em> expression is strongly associated with the lead AF GWAS variant at 5q31. However, the regulatory variant controlling <em>FAM13B</em> expression and the mechanism by which <em>FAM13B</em> impacts AF susceptibility are not known.</p> <p><strong>Methods</strong>: Bioinformatics, reporter gene transfections, gel shifts, and gene editing were used to identify the variant regulating <em>FAM13B</em> expression. RNAseq after <em>FAM13B</em> knockdown in stem cell-derived cardiomyocytes (iCMs) identified downstream processes. Patch clamp and calcium transient assays were used to assess downstream mechanisms. <em>Fam13b</em> knockout (KO) mice were studied for heart structural and functional changes, and pacing-induced arrhythmia.</p> <p><strong>Results</strong>: rs17171731 was identified as the regulatory variant controlling <em>FAM13B</em> expression, with decreased enhancer activity of the risk allele. Knockdown of <em>FAM13B</em> in iCMs altered expression of >1000 genes including <em>SCN2B</em> and led to pro-arrhythmogenic changes in the late sodium current and Ca<sup>2+</sup> cycling. FAM13B is a member of the Rho GTPase-activating protein (RhoGAP) gene family, but failed to demonstrate RhoGAP activity. GFP-tagged <em>FAM13B</em> expressed in iCMs localized at the Z-disc and plasma membrane. Fam13b knockout mice had increased basal p-wave duration and QT interval, and were more susceptible to pacing-induced arrhythmias vs. controls.</p> <p><strong>Conclusions</strong>: This study went from an AF GWAS locus to identify the causal variant and gene, mechanisms for this association, and confirmed arrhythmia susceptibility in <em>Fam13b</em> KO mice. FAM13B and downstream effectors are potential targets for patient-specific therapeutics.</p>
Data for A multi-phase biogeochemical model for mitigating earthquake-induced liquefaction via microbially induced desaturation and calcium carbonate precipitation
<p>This data accompanies the paper published in Biogeosciences, which can be found at https://doi.org/10.5194/egusphere-2022-1419.</p>
Faint calcium-rich transient from a double-detonation of a 0.6 M⊙ carbon-oxygen white dwarf star: Complimentary material
<p>Complimentary material to the manuscript.<br> The videos show the hydrodynamical evolution of the merger leading to the double-detonation.</p> <p>The movie "overdensity_formation.mp4" shows slices through the orbital plane color coded with the density (left) and the temperature (right), displaying frame by frame the formation of a cold, overdense fluid parcel after the disruption of the HeWD. <br> <br> The remaining movies show the overall evolution of different quantities with slices through the orbital plane on the left and perpendicular to the orbital plane on the right.</p>
Cortical astrocyte histamine-1-receptors regulate intracellular calcium and extracellular adenosine dynamics across sleep and wake
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Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages
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Data from: A comprehensive suite for extracting neuron signals across multiple sessions in one-photon calcium imaging
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Analysis dataset from: Simultaneous dual-color calcium imaging in freely-behaving mice
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Decreased FAM13B expression increases atrial fibrillation susceptibility by regulating sodium current and calcium handling
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CA3 axonal calcium imaging
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Accumbal calcium-permeable AMPA receptors orchestrate neuronal ensembles underlying social attachment
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Data-driven segmentation of cortical calcium dynamics
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Forest Inventory of a Calcium Amended Northern Hardwood Forest: Watershed 1, 2006, Hubbard Brook Experimental Forest
In order to evaluate the role of Ca supply in regulating the structure and function of base-poor forest and aquatic ecosystems, the Ca content of soil was increased through the application of wollastonite (CaSiO3) in October 1999. Forest inventory surveys were initiated in 1996 and repeated at 5 year intervals. This data set includes 2006 inventory measurements.The data consists of a total inventory of all trees >=10 cm diameter-at-breast-height (dbh) on the whole of the watershed (11.8 ha), as measured in each of the 200 25 m x 25 m plots. Trees >=2 to <=10 cm dbh were subsampled using a 3 meter wide strip along one edge of each 25 m x 25 m plot. With the addition of tree tags in 2006 on all trees >=10 cm dbh, tracking of individual trees is now possible. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.
Forest Inventory of a Calcium Amended Northern Hardwood Forest: Watershed 1, 2011, Hubbard Brook Experimental Forest
In order to evaluate the role of Ca supply in regulating the structure and function of base-poor forest and aquatic ecosystems, the Ca content of soil was increased through the application of wollastonite (CaSiO3) in October 1999. Forest inventory surveys were initiated in 1996 and repeated at 5 year intervals. This data set includes 2011 inventory measurements. The data consists of a total inventory of all trees >=10 cm diameter-at-breast-height (dbh) on the whole of the watershed (11.8 ha), as measured in each of the 200 25 m x 25 m plots. Trees >=2 to <=10 cm dbh were subsampled using a 3 meter wide strip along one edge of each 25 m x 25 m plot. With the addition of tree tags in 2006 on all trees >=10 cm dbh, tracking of individual trees is now possible. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.
Differential impacts of calcium and aluminum treatments on foliar and sapwood nutrition and metabolism of sugar maple trees growing at the west edge of WS6 of the Hubbard Brook Experimental Forest from 1997-2009.
Plot levels Ca and Al additions study at WS6 BACKGROUD: To better evaluate the interaction of Ca depletion and Al mobilization on a northern hardwood forest, the Nutrient Perturbation (NuPert) study was initiated in 1995, west of the biogeochemical reference watershed (W6) at the HBEF (43.95411°N, 71.74779°W). The study area is on a south-facing slope, with an elevational range of 700–760 m, and most soils are classified as either Aquic Haplorthods or Aquic Haplumbredts (Halman et al. 22015 and references therein). Twelve sugar maple (Acer saccharum Marshall) dominated plots (45 m × 45 m) were randomly assigned to one of three treatments (Ca addition, Al addition, or control (no addition)), yielding four replicates of each treatment in the study. In addition to sugar maple, American beech (Fagus grandifolia Ehrh.) and yellow birch (Betula alleghaniensis Britt.) are co-occurring tree species in these plots, whereas hobblebush (Viburnum lantanoides Michx.) and striped maple (Acer pensylvanicum L.) dominate the understory. Treatments began in 1995 with annual CaCl2 (2.5 g·m−2) and AlCl3 (0.9 g·m−2) applications occurring each fall or spring during leafless periods. The use of CaCl2 was halted in 1999 in favor of a one-time application (38 g·m−2) of wollastonite (CaSiO3, a slow-release form of Ca). Thereafter, AlCl3 additions occurred in alternate years in fall or spring. Foliage was collected from sunlit branches in the upper to mid canopy dominant sugar maple trees. Five trees per plot of each group were selected for sampling in August 2008. There was an ice storm in 1998 at this site which might have interfered with this study findings. In 2009, tiny sapwood plugs were also collected. All the analyses were carried out using the procedures described under section A (Ca-supplementation study). SUMMARY: Acid deposition induced losses of calcium (Ca) from northeastern forests have had negative effects on forest health for decades, including the mobilization of potentiall
Action potential evoked dendritic calcium signals in the medial superior olive (MSO) during development
<p>Dataset underlying the analysis of dendritic calcium signals evoked by somatic action potentials in MSO neurons during development (postnatal day 10 - 60). The data includes recordings from Mongolian gerbils (<em>Meriones unguiculatus</em>) raised in a normal acoustic environment and in omnidirectional white noise.</p> <p><strong>Linked paper:</strong> Franzen DL, Gleiss SA, Kellner CJ, Kladisios N, Felmy F. Activity-Dependent Calcium Signaling in Neurons of the Medial Superior Olive during Late Postnatal Development. J Neurosci. 2020 Feb 19;40(8):1689–700. <a href="https://doi.org/10.1523/JNEUROSCI.1545-19.2020">https://doi.org/10.1523/JNEUROSCI.1545-19.2020</a>.</p> <p><strong>Files included:</strong></p> <ul> <li> <p>A folder entitiled <em>data</em> containing:</p> <ul> <li>The imaging files (<code>.nix</code>) (1 file per imaged neuron). NIX files contain the kymograph data extracted from a ROI in the raw images along with the metadata. The imaging kymographs and associated <code>.nix</code> files were created with the <a href="https://zenodo.org/record/2575542#.XiYdAS2ZNo4">CaManager: ImageJ Plugin For Ca²⁺ Imaging</a>.</li> <li>An <code>excludes.csv</code> file that contains cells/trials to be excluded (e.g. recording error, movement, difficulty in tracing)</li> <li>A <code>Megatable_C.csv</code> file containing the electrophysiological data corresponding to imaged cells</li> </ul> </li> <li> <p>A <code>.nix</code> intermediate analysis file for <em>default parameters</em></p> </li> <li> <p>A <code>.csv</code> file with the combined electrophysiological and imaging data for <em>default parameters</em></p> </li> <li> <p>Several <code>.xlsx</code> files with the single cell data in Figures 2C, 2D, 2F.</p> </li> </ul> <p><strong>Jupyter notebook:</strong></p> <p>The <a href="https://github.com/delwen/CaJupyter">CaJupyter</a> GitHub repository contains a Jupyter notebook with a step-by-step guide of the analysis. It also allows for further exploration of the data.</p> <p><strong>See also:</strong></p> <ul> <li> <p>The original analysis code (without adaptations for the Jupyter notebook and Python 3) can be found at <a href="https://zenodo.org/record/2575675#.Xi%E2%80%A6">CaAnalysis: Analysis Toolbox for Ca²⁺ Imaging</a>.</p> </li> <li> <p>More information on NIX can be found at this <a href="https://g-node.github.io/nix/">link</a>.</p> </li> </ul>
Vitamin D and Calcium in Children 0-36 months: A Scoping Review of Health Outcomes
<p>We conducted a scoping review to describe the extent (size), range (variety), and nature (characteristics) of the evidence on published primary research and systematic reviews since 2008 relating to vitamin D or calcium intake (or biomarkers of intake) in relation to any health outcome in generally healthy infants and children less than 3 years old. The list of these set-aside studies with extracted data is available in Supplemental Data File 1. Data extracted from interventional studies are included in Supplemental Data File 2. Data extracted from observational studies are included in Supplemental Data File 3. </p>
Data from: Nonlinearities between inhibition and T-type calcium channel activity bidirectionally regulate thalamic oscillations
<p>Absence seizures result from 3-5 Hz generalized thalamocortical oscillations that depend on highly regulated inhibitory neurotransmission in the thalamus. Efficient reuptake of the inhibitory neurotransmitter GABA is essential, and reuptake failure worsens seizures. Here, we show that blocking GABA transporters (GATs) in acute brain slices containing key parts of the thalamocortical seizure network modulates epileptiform activity. As expected, we found that blocking either GAT1 or GAT3 prolonged oscillations. However, blocking both GATs unexpectedly suppressed oscillations. Integrating experimental observations into single-neuron and network-level computational models shows how a non-linear dependence of T-type calcium channel opening on GABA<sub>B</sub> receptor activity regulates network oscillations. Receptor activity that is either too brief or too protracted fails to sufficiently open T-type channels necessary for sustaining oscillations. Only within a narrow range does prolonging GABA<sub>B</sub> receptor activity promote channel opening and intensify oscillations. These results have implications for therapeutics that modulate GABA transporters.</p>
Large variations of Calcium isotopes in Lutao arc volcanic rocks: Influence of recycled marine carbonate and partial melting
<p>The dataset for the manuscript in preparation: Large variations of Calcium isotopes in Lutao arc volcanic rocks: Influence of recycled marine carbonate and partial melting</p>
Raw Data of Biodiesel production from waste cooking oil using calcium oxide (CaO) derived from Waste Scallop Shell
<p>The data are the results of laboratory tests on CaO catalysts made from scallop shells, testing the physicochemical, thermal and performance properties of biodiesel samples synthesized from waste cooking oil.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.