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FIGURE 6 in Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae)

FIGURE 6. Scales of (a), C. avicennae, GUIC CC1462M, paratype, 65.2 mm SL; (b), C. amphilekta, ZMMU P-3751, paratype, 68.3 mm TL (modified from Vasil'eva & Vasil'ev 2012).

FIGURE 3 in Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae)

FIGURE 3. Cobitis avicennae, GUIC CC1462MA, holotype, male, 68.4 mm SL, Iran, Gamasiab River (above: lateral view in life; below: dorsal view in preservative).

FIGURE 2 in Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae)

FIGURE 2. Maximum Likelihood (ML) estimation of the phylogenetic relationships based on the mitochondrial COI barcode region. Nucleotide positions with less than 95 % site coverage were eliminated before analysis. Numbers of major nodes indicate bootstrap values from the Maximum Likelihood-, Neighbour joining-, and Maximum Parsimony method from 1000 pseudo-replicates. All branch lengths (except to out-group) are drawn to scale and give number of substitutions per site.

FIGURE 4 in Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae)

FIGURE 4. Cobitis avicennae, GUIC CC1462M, paratypes, lateral and dorsal views, from top to bottom: 65.2, 65.8 and 80.9 mm SL, Iran, Gamasiab River.

FIGURE 8 in Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae)

FIGURE 8. Records of Cobitis species in the Persian Gulf, Kor and the southern Caspian Sea basins. C. amphilekta, C. avicennae, C. faridpaki, C. kellei, C. keyvani, C. linea.

FIGURE 7 in Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae)

FIGURE 7. Cobitis avicennae, first branched ray of pectoral fin showing lamina circularis, right side, dorsal view, GUIC CC1462M, paratype, 40.1 mm SL.

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FIGURE 2 in Cobitis keyvani, a junior synonym of Cobitis faridpaki (Teleostei: Cobitidae)

FIGURE 2. Cobitis faridpaki. (A) ZM-CBSU-CO1701C, male, 51.1 mm SL, (B) IMNRF-1016-A, male, 66.2 mm SL and (C) ZM-CBSU-CO1699C, female, 39.1 mm SL from Siahrud River, type locality of C. faridpaki, Iran

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FIGURE 4 in Cobitis keyvani, a junior synonym of Cobitis faridpaki (Teleostei: Cobitidae)

FIGURE 4. Cobitis faridpaki. (A) ZM-CBSU-CO1704C, female, 67.1 mm SL, (B) ZM-CBSU-CO1703C, female, 57.6 mm SL, (C) IMNRF-1015-A, male, 46.2 mm SL and (D) ZM-CBSU-CO1700C, female, 41.0 mm SL from Keselian River, type locality of C. keyvani, Iran

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FIGURE 6 in Cobitis keyvani, a junior synonym of Cobitis faridpaki (Teleostei: Cobitidae)

FIGURE 6. Bayesian consensus tree. Values at nodes correspond to BI posterior probability/ML bootstrap.

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FIGURE 1 in A new diminutive subterranean eel loach species of the genus Pangio (Teleostei: Cobitidae) from Southern India

FIGURE 1. Pangio pathala holotype (KUFOS.FT.2020.1, 32.1 mm SL) (A) in life, and (B–F) in preservative.

FIGURE 5 in Cobitis indus, a new spined loach from the Dalaman River in the Eastern Aegean Sea basin (Teleostei: Cobitidae)

FIGURE 5. Cobitis indus, paratypes; (a) NUIC 1708–2, female, 55.5 mm SL; (b) NUIC 1708–5, female, 64 mm SL; Turkey: Dalaman River at Alcı.

FIGURE 4 in Cobitis indus, a new spined loach from the Dalaman River in the Eastern Aegean Sea basin (Teleostei: Cobitidae)

FIGURE 4. Cobitis indus, paratypes; (a) NUIC 1708–2, female, 55.5 mm SL; (b) NUIC 1708–5, female, 64 mm SL; Turkey: Dalaman River at Alci.

FIGURE 1 in Cobitis indus, a new spined loach from the Dalaman River in the Eastern Aegean Sea basin (Teleostei: Cobitidae)

FIGURE 1. The Bayesian Inference (BI) phylogenetic relationships within the studied Cobitis based on the mitochondrial COI barcode region. Values at nodes correspond to BI posterior probability/ML bootstrap. Numbers after each species name corresponds to GenBank accession number on Table 1 (Solid bars right to the specimen labels indicate species delimitation results from PTP except outgroups).

FIGURE 8 in A new species of the genus Misgurnus (Cypriniformes, Cobitidae) from Ryukyu Islands, Japan

FIGURE 8. Type specimens of Misgurnus anguillicaudatus and five Misgurnus loaches described from Japan and Taiwan. a, BMNH 1843.7.21.15-18a (syntype of Cobitis anguillicaudata Cantor, 1842, Chusan Island [Zhoushan Island], China, 103.9 mm SL); b, BMNH 1843.7.21.15-18b (syntype of Cobitis anguillicaudata Cantor, 1842, 114.9 mm SL); c, BMNH 1843.7.21.15- 18c (syntype of Cobitis anguillicaudata Cantor, 1842, 87.0 mm SL); d, BMNH 1843.7.21.15-18d (syntype of Cobitis anguillicaudata Cantor, 1842, 91.5 mm SL); e, RMNH.PISC.2705 (lectotype of Cobitis rubripinnis Temminck & Schlegel, 1846, Nagasaki, Japan, 147.5 mm SL); f, BMNH1866.5.2.26 (lectotype of Cobitichthys enalios Bleeker, 1860, Kaminoseki, Japan, 64.6 mm SL); g, BMNH1866.5.2.96 (lectotype of Cobitichthys dichachrous Bleeker, 1860, Jedo [Tokyo], Japan, 102.8 mm SL); h, BMNH1866.5.2.24 (lectotype of Cobitichthys polynema Bleeker, 1860, Jedo [Tokyo], Japan, 123.6 mm SL); i, NRM 10354 (holotype of Misgurnus anguillicaudatus formosanus Rendahl, 1936, Lake Candidius, Taiwan, 105.1 mm SL). Photos by James Maclaine & Lucie Goodayle (BMNH) (a, b, c, d, f, g, h), Esther Dondorp (RMNH) (e), and Andrea Hennyey (NRM) (i).

FIGURE 7 in A new species of the genus Misgurnus (Cypriniformes, Cobitidae) from Ryukyu Islands, Japan

FIGURE 7. Lamina circularis of Northeast Asian species of Misgurnus. a, M. amamianus sp. nov. (KPM-NI 68404, holotype, Tokuno-shima Is., Japan, 83.8 mm SL); b, M. anguillicaudatus (Japan clade) (KYUM-PI 4520, Fukuoka, Japan, 93.4 mm SL); c, M. anguillicaudatus (China clade) (KMNH VR 100186, Zheijiang, China, 128.9 mm SL); d, M. dabryanus (JNC130, Fukuoka, Japan, 115.2 mm SL); e, M. mohoity (JNC563, Ibaraki, Japan, 71.7 mm SL); f, M. nikolskyi (JNC301, Tokyo, Japan, 121.3 mm SL); g, M. sp. (Clade A) (JNC345, Aomori, Japan, 110.5 mm SL); e, M. sp. OK (KMNH VR100189, Okinawa, Japan, 77.1 mm SL). Bar 1mm.

FIGURE 6 in A new species of the genus Misgurnus (Cypriniformes, Cobitidae) from Ryukyu Islands, Japan

FIGURE 6. Maximum likelihood tree of the partial sequences of mitochondrial DNA control region (CR) in Misgurnus amamianus sp. nov. and Japanese and Northeast Asian species of Misgurnus. CR sequences of Barbatula barbatula and Leptobotia elongata are used as outgroups. Numbers on each branch indicate bootstrap values with 1,000 replications.

FIGURE 5 in A new species of the genus Misgurnus (Cypriniformes, Cobitidae) from Ryukyu Islands, Japan

FIGURE 5. Misgurnus amamianus sp. nov., KPM-NI 68404, holotype. a, radiograph; b, radiograph of dorsal fin; c, radiograph of anal fin; d, radiograph of caudal fin; e, living body.

FIGURE 4 in A new species of the genus Misgurnus (Cypriniformes, Cobitidae) from Ryukyu Islands, Japan

FIGURE 4. Misgurnus amamianus sp. nov., KPM-NI 68404, holotype. a, mouth; b, lamina circularis (allow indicating the neckline at the base of lamina circularis), bar 1 mm; c, dorsal fin.

FIGURE 3 in A new species of the genus Misgurnus (Cypriniformes, Cobitidae) from Ryukyu Islands, Japan

FIGURE 3. Misgurnus amamianus sp. nov. a, holotype (male, KPM-NI 68404); b, paratype (female, TKPM-P 26178).

FIGURE 2 in A new species of the genus Misgurnus (Cypriniformes, Cobitidae) from Ryukyu Islands, Japan

FIGURE 2. Measurement methods of DPA (= the projected length from the insertion point of the pelvic fin to the anterior edge of the anal-fin base), LPC (= the projected length from the posterior edge of the anal-fin base to the base of the caudal fin), S (= the length of the second branched soft ray of dorsal fin), and L (= the length of last ray of dorsal fin).