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3,441 results for “Immune cells”
Single-Cell Mapping Reveals Several Immune Subsets Associated with Liver Metastasis of Pancreatic Ductal Adenocarcinoma
<p>Identifying a metastasis-correlated immune cell composition within the tumor microenvironment (TME) of pancreatic ductal adenocarcinoma (PDAC) will help to develop promising and innovative therapeutic strategies. Twenty-six samples from 11 patients (including 11 primary tumor tissues, 10 blood, and 5 lymph nodes) with different stages were used to develop a multiscale immune profile. High-dimensional single-cell analysis with mass cytometry was performed to search for metastasis-correlated immune changes in the microenvironment.</p> <p>The details about the files uploaded are as follows:</p> <p>1. panelA_Blood.zip includes 10 .fcs files from blood samples in Panel A;</p> <p>2. panelA_LN.zip includes 5 .fcs files from lymph node samples in Panel A;</p> <p>3. panelA_Tumor.zip includes 11 .fcs files from tumor tissue samples in Panel A;</p> <p>4. panelB_Tumor.zip includes 11 .fcs files from tumor tissue samples in Panel B;</p> <p>5. panel_metadata.xlsx describes marker used in Panel A and B;</p> <p>6. sample_metadata.xlsx describes detailed sample information.</p>
Data from: Desmoplastic stroma restricts T cell extravasation and mediates immune exclusion and immunosuppression in solid tumors
<p>In this study, we used our modified <span>precision-cut tumor slice assay of tumors treated with CAR T cells <em>in vivo </em></span><span>prior to harvest and harvested at different time-points post-treatment, which can better reflect the behavior of CAR T cells in the TME </span><em>in vivo</em><span>.</span></p>
Data from: Single-cell analysis identifies conserved features of immune dysfunction in simulated microgravity and spaceflight
<p>3-dimensional super-resolution microscopy volumes of human PBMCs recorded on a Zeiss LSM980 Airyscan2 laser scanning confocal microscope.</p> <p>Sample preparation and image capture:</p> <p>Live PBMCs were stained with 60 nM MitoTracker Red-CMX-Ros (ThermoFisher, Waltham, MA) either in 6-well plates or in the microgravity chambers for the last 2 hr of the microgravity simulation. At the end of the microgravity simulation cells were immediately fixed by 1:1 mixing the cell suspensions with 2× concentrated fixative (10% Sucrose (w/v) 120 mM KCl, 1% (w/v) glutaraldehyde, 8% (w/v) PFA pH 7.4) and incubated for 15 minutes at room temperature followed by 15 minutes on ice. Fixed cells were washed and stored in PBS until further staining for up to a week at 4 °C. 1 million fixed cells were resuspended in 1 mL of permeabilization solution (0.1% TritonX-100 in PBS) for 5 minutes. After twice washing in PBS, pellets were resuspended in 0.5 mL 1% BSA PBS containing Phalloidin-iFluor-488 (cat# ab176753, Abcam plc., Cambridge, UK) at the manufacturer’s recommended dilution, and were incubated for 90 minutes with gentle agitation. After washing in PBS, cells were stained with Hoechst 33342 (1 µg/mL in PBS) for 10 minutes. The fixed-stained cells were immobilized at 3 × 10<sup>5</sup> cells per well density in glass-bottom 96-well microplates (Greiner Bio-One, Monroe, NC), which were pre-coated with polyethyleneimine (1:15,000 (w/v)) for 16 hours in a 37 °C incubator, and washed twice with PBS. Microplates with the cell suspensions were centrifuged in a swing plate rotor centrifuge (Eppendorf 5810 R) at 400 × <em>g</em> and for 10 min and then fixed on the surface by adding an equal volume of 8% (w/v) PFA for 5 min. Finally, the fixative was replaced with 100 µL of antifade reagent (Vector Prolong Gold (ThermoFisher)). Samples were imaged immediately after this procedure on a Zeiss LSM980 Airyscan2 laser scanning confocal microscope (Carl Zeiss Microscopy, White Plains, NY). Single PBMCs were manually selected for recording based on low-resolution preview scans showing only nuclei. All singlet cells were selected in a small neighborhood to avoid biases. In each microscopy session, 24-40 cells were selected for recording in one well for each condition. This was performed in an interleaved manner, capturing 6-8 cells at a time, and then moving to the next well and then repeating this multiple times using the Experiment Designer module for automation. Super-resolution volumes of (358 × 358 × 70 pixels, 0.035 × 0.035 × 0.13 µm/voxel resolution) were recorded in the above-determined positions using Definite Focus autofocusing. A Plan-Apochromat 63 × 1.40 Oil lens, Airyscan2 SR (super-resolution) mode with optimal sampling and frame switching between 3 fluorescence channels to minimize spectral cross-bleed were used. MitoTracker Red, iFluor488, and Hoechs33342 were excited with 561, 488, and 405 nm solid-state lasers, respectively, using the optimal emission filter for each channel. 3D Airyscan2 processing was performed with standard filtering settings.</p> <p>File naming:</p> <p>Four zip files were deposited named as <Donor#id>.zip, where id goes from 1 to 4.</p> <p>Each zip file contains the following Zeiss Microscopy format image files: <Condition>_<Donor#id>_<Stain#batch>_<Cell>.czi</p> <p><Condition>:</p> <ul> <li>1G – Control culturing in 6-well plates for 25h</li> <li>uG – simulated microgravity culturing for 25h in NASA Rotating Wall Vessels</li> <li>1G+TLR – as above, with TLR 7/8 agonist (1 μM R848)</li> <li>uG+TLR– as above, with TLR 7/8 agonist (1 μM R848)</li> <li>1G+CyD– as above, with cytochalasin D</li> <li>uG+CyD– as above, with cytochalasin D</li> <li>1G+Q – as above, with quercetin 50µM</li> <li>uG+Q – as above, with quercetin 50µM</li> </ul> <p><Donor#id>: 1-4 indicates biological replicates</p> <p><Stain#batch>: 1-2 indicates experimental replicates of phalloidin staining and imaging session</p> <p><Cell>: arbitrary number to distinguish images within the same condition/donor/stain set.</p> <p>See image analysis pipelines used with these data at: https://github.com/gerencserlab/Superresolution-actin-and-mitochondria-analysis</p>
A Study of De-immunized DI-Leu16-IL2 Administered Subcutaneously in Participants With B-cell NHL
ClinicalTrials.gov study NCT01874288. IPD Sharing: NO. Countries: 1. Publications: 3.
Chemotherapy and Unrelated Donor Stem Cell Transplantation for Patients With Cancers of the Blood and Immune System
ClinicalTrials.gov study NCT00520130. IPD Sharing: NO. Countries: 1. Publications: 6.
Study of Infusion of Blood Cells (Lymphocytes) to Stimulate the Immune System to Fight Leukemia/Lymphoma
ClinicalTrials.gov study NCT01685606. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Cellular Immunity and Renal Cell Cancer
ClinicalTrials.gov study NCT04377113. IPD Sharing: NO. Countries: 1. Publications: 9.
Genomic Responses of Human Immune and Non-Immune Cells to Glucocorticoids
ClinicalTrials.gov study NCT02798523. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Accelerated Immunization to Induce Cytomegalovirus Immunity in Stem Cell Donors
ClinicalTrials.gov study NCT00353977. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Effect of Natural Food on Gut Microbiome and Phospholipid Spectrum of Immune Cells in COVID-19 Patients
ClinicalTrials.gov study NCT05970861. IPD Sharing: Not stated. Countries: 1. Publications: 2.
A Study of Atezolizumab in Combination With Cabozantinib Compared to Cabozantinib Alone in Participants With Advanced Renal Cell Carcinoma After Immune Checkpoint Inhibitor Treatment
ClinicalTrials.gov study NCT04338269. IPD Sharing: YES. Countries: 15. Publications: 2.
Open-Label Extension Study of De-immunized DI-Leu16-IL2 Immunocytokine Administered in Participants With B-cell NHL
ClinicalTrials.gov study NCT02151903. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Donor Stem Cell Transplant With No or Low-Intensity Chemotherapy Using Sirolimus and Treated Immune Cells to Treat Blood and Lymph Cancers
ClinicalTrials.gov study NCT00074490. IPD Sharing: NO. Countries: 1. Publications: 3.
Study to Optimize the Quality of Samples for Cell-mediated Immunity (CMI) in ART-naïve HIV-1-infected Subjects
ClinicalTrials.gov study NCT01610427. IPD Sharing: NO. Countries: 1. Publications: 1.
B-cell Immunity to Influenza (SLVP017)- Year 1, 2009
ClinicalTrials.gov study NCT02133781. IPD Sharing: NO. Countries: 1. Publications: 6.
Humoral and Cell Mediated Immunity and Safety of MF59C.1-adjuvanted Subunit Influenza Vaccine or a Conventional Subunit Influenza Vaccine in Previously Unvaccinated Healthy Subjects
ClinicalTrials.gov study NCT01342796. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Safety and Effectiveness of a Vaccine for Prostate Cancer That Uses Each Patients' Own Immune Cells.
ClinicalTrials.gov study NCT00289341. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Avelumab In Combination Regimens That Include An Immune Agonist, Epigenetic Modulator, CD20 Antagonist and/or Conventional Chemotherapy in Patients With Relapsed or Refractory Diffuse Large B-cell Lym
ClinicalTrials.gov study NCT02951156. IPD Sharing: YES. Countries: 8. Publications: 1.
Study of Gene Modified Immune Cells in Patients With Advanced Melanoma
ClinicalTrials.gov study NCT00910650. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Immune Intervention With Rituximab to Preserve Beta Cell Function in Early Onset Type 1 Diabetes
ClinicalTrials.gov study NCT01280682. IPD Sharing: Not stated. Countries: 1. Publications: 2.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.