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65 results for “Lactococcus”
Target identification of the cold stress induced sRNA CisR in Lactococcus lactis
GEO Series GSE115974. Lactococcus cremoris. 8 samples. Type: Expression profiling by high throughput sequencing.
Mobile restriction-modification systems are key pillars of the antiphage architecture of Lactococcus
<p><span>The genomes of 43 distinct strains of dairy lactococci were reconstructed by a combination of long- and short-read sequencing technologies, resolving the plasmid complement and methylome of these strains in full. The genomes comprised 43 chromosomes of approximately 2.5 Mb each and 269 plasmids ranging in size from 2 – 211 kb (at an average occurrence of 6 per strain). A total of 936 antiphage genes present in all strains were catalogued and a cross-correlation of identified genes with co-occurrent mobile elements was performed, which determined that over 50 % of these genes can be considered mobile. Detailed analysis established that restriction-modification (R-M) systems form a significant portion of this mobile phage defensome. We therefore undertook to assign each detected methylated motif (as associated with Type I, II and III R-M systems) to the specific gene(s) on the chromosome or plasmidome encoding the methylating enzyme through both homology searching and molecular cloning. The cumulative antiphage activity of selected systems and the ability of truncated R-M genes to contribute to methylation were demonstrated. This study reveals the dairy lactococcal plasmidome to be a rich reservoir of Type I R-M specificity subunit encoding genes, and the implications for complete bacterial genome sequencing, methylation analysis, dairy strain selection, phage defence, plasmid prevalence, plasmid mobility, and the wider antiphage landscape are discussed.</span></p>
Expression analysis of strains Lactococcus lactis KCTC 3769T, L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungan
GEO Series GSE58002. Lactococcus lactis; Pseudolactococcus piscium; Pseudolactococcus raffinolactis; Pseudolactococcus chungangensis; Lactococcus garvieae; Pseudolactococcus plantarum; Lactococcus fujiensis. 7 samples. Type: Expression profiling by array.
Expression analysis of Lactococcus lactis NIAI712 and its plasmid cured variant
GEO Series GSE48143. Lactococcus lactis; Lactococcus cremoris. 2 samples. Type: Expression profiling by array.
ChIP-seq for transcriptional regulator CodY of Lactococcus lactis
GEO Series GSE139902. Lactococcus lactis. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
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