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260 results for “Nicotiana”
Dataset of results for a copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana.
<p>CRISPR-based programmable transcriptional activators (PTAs) are used in plants for rewiring gene networks. Better tuning of their activity in a time and dose-dependent manner should allow precise control of gene expression. Here, we report the optimization of a Copper Inducible system called CI-switch for conditional gene activation in Nicotiana benthamiana. In the presence of copper, the copper-responsive factor CUP2 undergoes a conformational change and binds a DNA motif named copper-binding site (CBS). In this study, we tested several activation domains fused to CUP2 and found that the non-viral Gal4 domain results in strong activation of a reporter gene equipped with a minimal promoter, offering advantages over previous designs. To connect copper regulation with downstream programmable elements, several copper-dependent configurations of the strong dCasEV2.1 PTA were assayed, aiming at maximizing activation range, while minimizing undesired background expression. The best configuration involved a dual copper regulation of the two protein components of the PTA, namely dCas9:EDLL and MS2:VPR, and a constitutive RNA pol III-driven expression of the third component, a guide RNA with anchoring sites for the MS2 RNA-binding domain. With these optimizations, the CI/dCasEV2.1 system resulted in copper-dependent activation rates of 2,600-fold and 245-fold for the endogenous N. benthamiana DFR and PAL2 genes, respectively, with negligible expression in the absence of the trigger. The tight regulation of copper over CI/dCasEV2.1 makes this system ideal for the conditional production of plant-derived metabolites and recombinant proteins in the field.</p>
Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor SlCYS8 in Nicotiana benthamiana
<p>Complete dataset (MS Label-free quantification) for the publication 'Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilizing inhibitor <em>Sl</em>CYS8 in <em>Nicotiana benthamiana</em>'</p> <p>DOI: 10.1111/pbi.13092</p> <p> </p>
Reproductive transcriptome of Nicotiana tabacum male gametophyte unveils decreasing number of new transcription factors during pollen ontogeny
<p>Plants with highly reduced male gametophytes represent a successful adaptation to sexual reproduction, which plays an important role in the colonization and radiation of terrestrial ecosystems. During pollen maturation, microsporocytes to mature pollen grain cells switch from mitosis to meiosis and ultimately form a haploid male gametophyte, a widely used model to study plant development. We performed RNASeq analysis of <em>Nicotiana tabacum</em> at six developmental stages from microspores to mature pollen grain to characterize in detail key transcription factor (TF) genes involved in pollen ontogeny. Our results provide the most complete transcriptomic data during pollen development in the important model plant <em>Nicotiana tabacum. </em>We have identified DEGs associated with six ontogenetic stages of the male gametophyte, providing insights into the molecular regulation of reproductive development by TFs that have a high potential for agronomic research investigating molecular networks associated with pollen sterility and related issues.</p>
Pan-metabolome of the genus Nicotiana", Mendeley Data, V1, doi: 10.17632/rhnxrfzm6n.1
<p>Raw data from metabolite analysis of 20 Nicotiana species. LC-ESI-QTof analysis of polar extracts of leaf, GC-MS analysis of polar and non-polar extracts of leaf, UPLC analysis of carotenoids and chlorophylls in leaf and SPME-GC-MS analysis of freeze dried leaf powder.</p>
Fig. 7. Nicotiana insecticida. A in 994. NICOTIANA INSECTICIDA
Fig. 7. Nicotiana insecticida. A, flower, × 3; B, corolla, face view, opened out, × 3; C, corolla, opened out, × 3; D, style and ovary, × 3; E, leaf, × 3/; F, habit, × 1/; G, upper stem, × 9; H, 4 6 fruit, with calyx, × 3; I, fruit, calyx removed, × 3; J, stem hairs showing dead insects, × 3; K, calyx hairs, × 9. Drawn by Deborah Lambkin from specimens cultivated at Kew.
Fig. 5 in 994. NICOTIANA INSECTICIDA
Fig. 5. Nicotiana insecticida in Palm Valley, Northern Territory, growing on the south side of an overhanging rock. Photograph Maarten Christenhusz.
Fig. 4 in 994. NICOTIANA INSECTICIDA
Fig. 4. The author (MJMC) in Palm Valley, Northern Territory, with the palm Livistona mariae, for which this area is famous. Photograph Mark Chase.
Fig. 2 in 994. NICOTIANA INSECTICIDA
Fig. 2. Habit of Nicotiana insecticida growing under Acacia along the Northwest Coastal Hwy, just south of Minilya Roadhouse, Western Australia. Photograph Maarten Christenhusz.
Fig. 3 in 994. NICOTIANA INSECTICIDA
Fig. 3. Nicotiana insecticida, Roy Hill Station, Western Australia, growing in a cattle pasture where the main inflorescence has been eaten by the cattle. Photograph Maarten Christenhusz.
Fig. 6 in 994. NICOTIANA INSECTICIDA
Fig. 6. Nicotiana insecticida at Lasseter's Cave, Northern Territory, growing in the opening of a small cave. Photograph Maarten Christenhusz.
Fig. 1 in 994. NICOTIANA INSECTICIDA
Fig. 1. Close-up of the hairs on the stems and leaves of Nicotiana insecticida, Minilya Roadhouse, Western Australia, showing aphids caught by the ellipsoidoidal gland-bearing, short hairs. Photograph Maarten Christenhusz.
Nicotiana benthamiana and virus genomic references
<p>Nicotiana benthamiana curated and modified genomic references.</p> <p>TSWV viral references.</p>
Image Repository for Excessive leaf oil modulates the plant abiotic stress response via reduced stomatal aperture in tobacco (Nicotiana tabacum)
<p>This repository contains raw image data from <em>Nicotiana tabacum</em>, associated with a publication related to wild-type and high lipid producing (HLP) tobacco varieties. Sample preparation methods and associated analysis can be found in the associated publication. For information and details, find more on the project Github: https://github.com/danforthcenter/tobacco-heat-paper.</p> <p><strong>Contact information: </strong></p> <p>Katherine M. Murphy, Donald Danforth Plant Science Center, kmurphy@danforthcenter.org</p>
Fig. 30 in Nine new species of Australian Nicotiana (Solanaceae)
Fig. 30. Nicotiana erytheia based on living plants cultivated at the Royal Botanic Gardens, Kew, grown from seeds associated with Chase & Christenhusz 19119, North West Coastal Highway, north of the Billabong Roadhouse, Western Australia. Painted by Deborah Lambkin (reproduced from Curtis' Botanical Magazine with permission).
Fig. 27 in Nine new species of Australian Nicotiana (Solanaceae)
Fig. 27. Nicotiana karara, based on living plants cultivated at the Royal Botanic Gardens, Kew, grown from seeds associated with the holotype, Chase & Christenhusz 68280 (PERTH), Mungada Road to the Karara Mine, Western Australia. Drawn by Deborah Lambkin. (a) Corolla split to show positions of didynamous anthers. (b) Floral limb, face-on. (c) Carpel, style and stigma. (d) Flower, side view. (e) Pubescence, leaf lamina. (f) Pubescence, leaf margin. (g) Pubescence, upper stem. (h) Pubescence on middle stem. (i) Pubescence on lower stem. (j) Mature capsule. (k) Capsule with calyx removed. (l) Stem leaf. (m) Habit. Scale bars: 1.0 cm (a–d, e–k); 3.0 cm (l); plant in (m) 88 cm tall.
Fig. 29 in Nine new species of Australian Nicotiana (Solanaceae)
Fig. 29. Map of distributions of Nicotiana karara (red circles), N. rosulata (purple circles) and N. erytheia (green circles) in Western Australia. Created by Maarten Christenhusz from data downloaded from the Australasian Virtual Herbarium website.
Fig. 23 in Nine new species of Australian Nicotiana (Solanaceae)
Fig. 23. (a) Habit of Nicotiana olens (Chase & Christenhusz 17017), Whitton Stock Route Road, north of Yenda, New South Wales. (b) Close-up of inflorescence of N. olens (Chase & Christenhusz 17017).
Fig. 22 in Nine new species of Australian Nicotiana (Solanaceae)
Fig. 22. Habitat of Nicotiana olens (Chase & Christenhusz 17018) in Cocoparra National Park, Woolshed Flat Trail, New South Wales. Photo by Maarten Christenhusz.
Fig. 26 in Nine new species of Australian Nicotiana (Solanaceae)
Fig. 26. Nicotiana bungonia based on living plants cultivated at the Royal Botanic Gardens, Kew, grown from seeds associated with the holotype Taseski 1398 (NSW), vouchered as Chase & Christenhusz 21011 (K). Drawn by Deborah Lambkin from a plant grown from seeds of the type collection. (a) Floral limb, face-on. (b) Flower, side view. (c) Floral tube split to show positions of anthers. (d) Carpel, style and stigma. (e) Mature capsule. (f) Fruit with calyx removed. (g) Pubescence on leaf lamina near midvein. (h) Pubescence on leaf margin. (i) Pubescence on calyx. (j) Pubescence on upper stem. (k) Stem leaf. (l) Habit. Scale bars: 1.0 cm (a–f); 3.3 mm (g–i); 1.0 cm (j); 3.0 cm (k); the plant in (l) is 112 cm tall.
Fig. 17 in Nine new species of Australian Nicotiana (Solanaceae)
Fig. 17. Habitats of Nicotiana latifolia. (a) Wills Developmental Road, Leichhardt River Bridge, Queensland. (b) Barkly Highway, Spear River Bridge, near Mount Isa Airport, Queensland. Photographs by Maarten Christenhusz.
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