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Figure 12 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 12. Majority rule (50%) consensus tree of five most parsimonious trees showing character tracing. Numbers refer to characters listed in the text. Characters printed in bold and italic face presented at least one instance of reversal.

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Figure 10 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 10. Roboastra leonis sp. nov. (CASIZ 097577). A, scanning electron micrograph of the oral tube, scale bar = 2 mm. The drawing shows the oral tube opened and the presence of a pair of elongate pouches opening into the digestive system. B, detail of the right elongate pouche, scale bar = 1 mm. Abbreviations: ep, elongate pouches; lc, labial cuticle; m, mouth; ot, oral tube.

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Figure 9 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 9. Roboastra leonis sp. nov. General arrangement of the internal organs. Scale bar = 1 mm. Abbreviations: bb, buccal bulb; bbm, buccal bulb muscle; bgl, blood gland; ca, cephalic artery; cg, cerebral ganglion; hd, hermaphroditic duct; hg+dg, hermaphrodite gland + digestive gland; in, intestine; nrh, rhinophoral nerve; oe, oesophagus; ot, oral tube; otm, oral tube muscle; pe, pericardium; rh, rhinophore; rs, renal syrinx; vd, vas deferens; ve, ventricle; vgl, vaginal gland.

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Figure 7 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 7. Scanning electron micrographs of the radula. A, right half of the radula, Roboastra luteolineata (CASIZ 115739), scale bar = 300 Mm. B, innermost lateral teeth, R. luteolineata (CASIZ 086319), scale bar = 30 Mm. C, left half of the radula, R. tigris (CASIZ 057321), scale bar = 300 Mm. D, outermost lateral teeth, R. tigris (CASIZ 068357), scale bar = 90 Mm. E, innermost lateral teeth, R. europaea (CASIZ 166053); white lines indicate the bifid upper cusp of some inner teeth, scale bar = 200 Mm. F, right half of the radula, R. caboverdensis (MNCN 15.05/46614), scale bar = 300 Mm. G, innermost lateral teeth, R. caboverdensis (MNCN 15.05/46614), scale bar = 90 Mm. H, right half of the radula, R. leonis sp. nov. (CASIZ 097577), scale bar = 300 Mm.

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Figure 8 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 8. Half row of radula of two specimens of: A, Roboastra arika Burn, 1967 and B, R. luteolineata (Baba, 1936). Redrawn from their original descriptions.

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Figure 11 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 11. Majority rule (50%) consensus tree of the phylogenetic relationships of the genus Roboastra. Numbers above the branches refer to percentage of trees supporting the branch, numbers below to the bootstrap support values.

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Figure 5. Roboastra rubropapulosa. A in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 5. Roboastra rubropapulosa. A, original painting of Nembrotha rubropapulosa (Bergh, 1905). B, photograph of the living animal identified as R. rubropapulosa by T.M. Gosliner (Gosliner et al., 1996). C, right half of the radula of Nembrotha sp. (CASIZ 106531), previously identified as R. rubropapulosa. Scale bar = 90 Mm.

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Figure 3 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 3. Drawings of reproductive systems of Roboastra spp. A, R. gracilis. B, R. luteolineata. C, R. tigris. D, R. europaea (adapted from Pola et al., 2003). E, R. caboverdensis. F, R. leonis sp. nov. Scale bar = 1 mm. Abbreviations: am, ampulla; bc, bursa copulatrix; fglm, female gland mass; ga, genital atrium; p, penis; pr, prostate; rs, receptaculum seminis, ud, uterine duct; va, vagina; vd, vas deferens; vgl, vaginal gland.

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Figure 6 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 6. Photographs of living animals: A, Roboastra luteolineata, Papua New Guinea (photo T.M. Gosliner). B, R. tigris, Mar de Cortés (photo H. Debelius). C1, R. europaea, Carroñera, Spain (light form) (photo L. Tocino). C2, R. europaea, Tarifa, Spain (black form) (photo T.M. Gosliner. D, R. caboverdensis, Cape Verde (photo P. Wirtz). E, R. leonis sp. nov., the Galápagos Islands (photo T.M. Gosliner).

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Figure 1 in Review of the systematics of the genus Roboastra Bergh, 1877 (Nudibranchia, Polyceridae, Nembrothinae) with the description of a new species from the Galápagos Islands

Figure 1. Roboastra gracilis: living specimens from: A, Fiji (photo K. Tucker); B, Cebu, Philippines (photo M. Miller); C, Sulawesi, Indonesia (photo L. Warren); and D, Hawaii (photo P. Fiene).

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FIG. 12 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 12. Penial morphology in Cadlina spp. A. Cadlina sp. 3, MIMB47975. B. Cadlina umiushi, MIMB47996. C. Cadlina laevis, MIMB47963. Scale bars: A, C = 100 µm; A', B' = 50 µm; C = 200 µm; C' = 20 µm. РИС. 12. МорфологиЯ пениса Cadlina spp. A. Cadlina sp. 3, MIMB47975. B. Cadlina umiushi, MIMB47996. C. Cadlina laevis, MIMB47963. Масштабные линейки: A, C = 100 мкм; A', B' = 50 мкм; C = 200 мкм; C' = 20 мкм.

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FIG. 11 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 11. Configuration of male and female reproductive organs in Cadlina spp., female gland mass removed. A. Cadlina laevis, MIMB47963. B. Cadlina umiushi, MIMB47996. C. Cadlina sp. 3, MIMB47975. D. Cadlina sp. 4, MIMB47978. E. Cadlina sp. 1, MIMB47971. F. Cadlina sp. 7, MIMB47981. G. Cadlina sp. 6, MIMB47980. Abbreviations: amp = ampulla; bc = bursa copulatrix; ps = penial sheath; pvd = prostatic vas deferens; rs = receptaculum seminis; va = vagina. Scale bar: 1 mm. РИС. 11. МорфологиЯ муЖских и Женских репродуктивных органов Cadlina spp., комплекс Женских ЖелеЗ удален. A. Cadlina laevis, MIMB47963. B. Cadlina umiushi, MIMB47996. C. Cadlina sp. 3, MIMB47975. D. Cadlina sp. 4, MIMB47978. E. Cadlina sp. 1, MIMB47971. F. Cadlina sp. 7, MIMB47981. G. Cadlina sp. 6, MIMB47980. СокраЩениЯ: amp = ампулла; bc = копулЯтивнаЯ сумка; ps = мешок пениса; pvd = простатический семЯпровод; rs = семЯприемник; va = вагина. МасштабнаЯ линейка: 1 мм.

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FIG. 10 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 10. Buccal armature of Cadlina umiushi from the Sea of Japan (SEM). A. MIMB47995, radula. B. MIMB47995, anterior radular portion, rachidian and inner lateral teeth. C. MIMB47995, outer lateral teeth. D. MIMB47995, radula on odontophore. E. MIMB47995, labial cuticle. F. MIMB47995, labial cuticle elements. G. MIMB47996, radula. H. MIMB47996, rachidian and innermost lateral teeth. I. MIMB47996, outer lateral teeth. Scale bars: A = 500 µm; B, C, I = 50 µm; D, E, G = 200 µm; F = 10 µm; H = 20 µm. РИС. 10. Буккальное вооруЖение Cadlina umiushi иЗ Японского морЯ (СЭМ). А. MIMB47995, радула. B. MIMB47995, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. С. MIMB47995, внешние латеральные Зубы. D. MIMB47995, радула на одонтофоре. Е. MIMB47995, лабиальнаЯ кутикула. F. MIMB47995, Элементы лабиальной кутикулы. G. MIMB47996, радула. H. MIMB47996, центральные и внутренние латеральные Зубы. I. MIMB47996, внешние латеральные Зубы. Масштабные линейки: A = 500 мкм; B, C, I = 50 мкм; D, E, G = 200 мкм; F = 10 мкм; Н = 20 мкм.

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FIG. 9 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 9. Buccal armature of Cadlina sp. 6 (MIMB47980, Shikotan Is., Sea of Okhotsk) and Cadlina sp. 5 (MIMB47979, Iturup Is., Sea of Okhotsk) (SEM). A. Cadlina sp. 6, anterior radular portion. B. Cadlina sp. 6, rachidian and inner lateral teeth. C. Cadlina sp. 6, rachidian and innermost lateral teeth. D. Cadlina sp. 6, middle radular portion. E. Cadlina sp. 6, outer lateral teeth. F. Cadlina sp. 6, rachidian and innermost lateral teeth. G. Cadlina sp. 6, radula on odontophore. H. Cadlina sp. 6, labial cuticle. I. Cadlina sp. 6, labial cuticle elements. J. Cadlina sp. 5, radula. K. Cadlina sp. 5, rachidian and inner lateral teeth. L. Cadlina sp. 5, rachidian teeth. M. Cadlina sp. 5, outer lateral teeth. N. Cadlina sp. 5, radula on odontophore. O. Cadlina sp. 5, labial cuticle. P. Cadlina sp. 5, labial cuticle rodlets. Scale bars: A, N, O = 100 µm; B, E, M = 50 µm; C, F, I, L = 10 µm; D, G, H, J = 200 µm; K, P = 20 µm. РИС. 9. Буккальное вооруЖение Cadlina sp. 6 (MIMB47980, о. Шикотан, Охотское море) и Cadlina sp. 5 (MIMB47979, о. Итуруп, Охотское море) (СЭМ). A. Cadlina sp. 6, переднЯЯ часть радулы. B. Cadlina sp. 6, центральные и внутренние латеральные Зубы. C. Cadlina sp. 6, центральные и внутренние латеральные Зубы. D. Cadlina sp. 6, среднЯЯ часть радулы. E. Cadlina sp. 6, внешние латеральные Зубы. F. Cadlina sp. 6, центральные и внутренние латеральные Зубы. G. Cadlina sp. 6, радула на одонтофоре. H. Cadlina sp. 6, лабиальнаЯ кутикула. I. Cadlina sp. 6, Элементы лабиальной кутикулы. J. Cadlina sp. 5, радула. К. Cadlina sp. 5, центральные и внутренние латеральные Зубы. L. Cadlina sp. 5, центральные Зубы. М. Cadlina sp. 5, внешние латеральные Зубы. N. Cadlina sp. 5, радула на одонтофоре. О. Cadlina sp. 5, лабиальнаЯ кутикула. P. Cadlina sp. 5, Элементы лабиальной кутикулы. Масштабные линейки: A, N, O = 100 мкм; B, E, M = 50 мкм; C, F, I, L = 10 мкм; D, G, H, J = 200 мкм; К, Р = 20 мкм.

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FIG. 8 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 8. Buccal armature of Cadlina sp. 7 (MIMB47981, Sea of Japan) and Cadlina sp. 4 (MIMB47978, Sea of Japan) (SEM). А. Cadlina sp. 7, radula. B. Cadlina sp. 7, anterior radular portion, rachidian and inner lateral teeth. C. Cadlina sp. 7, outer lateral teeth. D. Cadlina sp. 7, rachidian and innermost lateral teeth. E. Cadlina sp. 4, radula. F. Cadlina sp. 4, anterior radular portion, rachidian and inner lateral teeth. G. Cadlina sp. 4, rachidian and innoermost lateral teeth. H. Cadlina sp. 4, outer lateral teeth. I. Cadlina sp. 4, labial cuticle rodlets. Scale bars: A = 300 µm; B, F = 100 µm; C = 30 µm; D = 10 µm; E = 400 µm; G, I = 20 µm; H = 50 µm. РИС. 8. Буккальное вооруЖение Cadlina sp. 7 (MIMB47981, Японское море) и Cadlina sp. 4 (MIMB47978, Японское море) (СЭМ). А. Cadlina sp. 7, радула. B. Cadlina sp. 7, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. C. Cadlina sp. 7, наруЖные латеральные Зубы. D. Cadlina sp. 7, центральные и внутренние латеральные Зубы. Е. Cadlina sp. 4, радула. F. Cadlina sp. 4, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. G. Cadlina sp. 4, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. H. Cadlina sp. 4, внешние латеральные Зубы. I. Cadlina sp. 4, Элементы лабиальной кутикулы. Масштабные линейки: A = 300 мкм; B, F = 100 мкм; С = 30 мкм; D = 10 мкм; Е = 400 мкм; G, I = 20 мкм; Н = 50 мкм.

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FIG. 7 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 7. Buccal armature of Cadlina sp. 3 from the Sea of Japan (SEM). A. MIMB47975, radula. B. MIMB47975, anterior radular portion, rachidian and inner lateral teeth. C. MIMB47975, anterior radular portion, outer lateral teeth. D. MIMB47976, radula. E. MIMB47976, anterior radular portion, rachidian and inner lateral teeth. F. MIMB47976, anterior radular portion, outer lateral teeth. G. MIMB47976, odontophore with radula. H. MIMB47976, labial cuticle. I. MIMB47976, labial cuticle rodlets. J. MIMB47973, anterior radular portion, rachidian and inner lateral teeth. K. MIMB47973, anterior radular portion, outer lateral teeth. L. MIMB47973, labial cuticle rodlets. Scale bars: A, D, H = 500 µm; B, C, F, J = 50 µm; E = 100 µm; G = 200 µm; I, L = 10 µm; K = 20 µm. РИС. 7. Буккальное вооруЖение Cadlina sp. 3 иЗ Японского морЯ (SEM). А. MIMB47975, радула. B. MIMB47975, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. С. MIMB47975, переднЯЯ часть радулы, внешние латеральные Зубы. D. MIMB47976, радула. Е. MIMB47976, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. F. MIMB47976, переднЯЯ часть радулы, внешние латеральные Зубы. G. MIMB47976, одонтофор с радулой. Н. MIMB47976, лабиальнаЯ кутикула. I. MIMB47976, Элементы лабиальной кутикулы. J. MIMB47973, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. К. MIMB47973, переднЯЯ часть радулы, внешние латеральные Зубы. L. MIMB47973, Элементы лабиальной кутикулы. Масштабные линейки: A, D, H = 500 мкм; B, C, F, J = 50 мкм; Е = 100 мкм; G = 200 мкм; I, L = 10 мкм; К = 20 мкм.

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FIG. 6 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 6. Buccal armature of Cadlina sp. 1 (MIMB47971, Urup Is., Sea of Okhotsk) and Cadlina sp. 2 (MIMB42230, Iturup Is., Sea of Okhotsk) (SEM). A. Cadlina sp. 1, radula. B. Cadlina sp. 1, anterior radular portion, rachidian and lateral teeth. C. Cadlina sp. 1, rachidian and innermost lateral teeth. D. Cadlina sp. 1, outer lateral teeth. E. Cadlina sp. 1, outer lateral teeth. F. Cadlina sp. 1, labial cuticle rodlets. G. Cadlina sp. 2, anterior radular portion, rachidian and inner lateral teeth. H. Cadlina sp. 2, outer lateral teeth. I. Cadlina sp. 2, labial cuticle rodlets. Scale bars: A = 500 µm; B = 300 µm; C = 50 µm; D, E = 100 µm; F = 20 µm; G, H = 30 µm; I = 10 µm. РИС. 6. Буккальное вооруЖение Cadlina sp. 1 (MIMB47971, о. Уруп, Охотское море) и Cadlina sp. 2 (MIMB42230, о. Итуруп, Охотское море) (СЭМ). А. Cadlina sp. 1, радула. B. Cadlina sp. 1, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. C. Cadlina sp. 1, центральные и внутренние латеральные Зубы. D. Cadlina sp. 1, внешние латеральные Зубы. Е. Cadlina sp. 1, внешние латеральные Зубы. F. Cadlina sp. 1, Элементы лабиальной кутикулы. G. Cadlina sp. 2, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. H. Cadlina sp. 2, внешние латеральные Зубы. I. Cadlina sp. 2, Элементы лабиальной кутикулы. Масштабные линейки: A = 500 мкм; B = 300 мкм; С = 50 мкм; D, Е = 100 мкм; F = 20 мкм; G, Н = 30 мкм; I = 10 мкм.

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FIG. 5 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 5. Buccal armature of Cadlina laevis s.str., specimens from the White Sea (SEM). A. MIMB47939, radula. B. MIMB47939, anterior radular portion, rachidian and inner lateral teeth. C. MIMB47939, same as B, enlarged. D. MIMB47939, anterior radular portion, outer lateral teeth. E. MIMB47939, odontophore with radula. F. MIMB47939, labial cuticle. G. MIMB47939, labial cuticle rodlets. H. MIMB47963, radula. I. MIMB47963, middle radular portion, rachidian and inner lateral teeth. K. MIMB47963, middle radular portion, rachidian and innermost laterals. L. MIMB47963, outer lateral teeth. M. MIMB47963, odontophore with radula. N. MIMB47963, labial cuticle. O. MIMB47963, labial cuticle rodlets. Scale bars: A, E, H = 200 µm; B, D, I = 50 µm; C, K, L, N = 20 µm; F, M = 100 µm; G = 10 µm; O = 5 µm. РИС. 5. Буккальное вооруЖение Cadlina laevis s.str., особи иЗ Белого морЯ (СЭМ). A. MIMB47939, радула. B. MIMB47939, переднЯЯ часть радулы, центральные и внутренние латеральные Зубы. C. MIMB47939, то Же, что B, увеличенное. D. MIMB47939, переднЯЯ часть радулы, внешние латеральные Зубы. E. MIMB47939, одонтофор с радулой. F. MIMB47939, лабиальнаЯ кутикула. G. MIMB47939, родлеты лабиальной кутикулы. H. MIMB47963, радула. I. MIMB47963, среднЯЯ часть радулы, центральные и внутренние латеральные Зубы. K. MIMB47963, среднЯЯ часть радулы, центральные и внутренние латеральные Зубы. L. MIMB47963, наруЖные латеральные Зубы. М. MIMB47963, одонтофор с радулой. N. MIMB47963, лабиальнаЯ кутикула. O. MIMB47963, родлеты лабиальной кутикулы. Масштабные линейки: A, E, H = 200 мкм; B, D, I = 50 мкм; С, К, L, N = 20 мкм; F, М = 100 мкм; G = 10 мкм; О = 5 мкм.

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FIG. 3 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 3. COI haplotype network of Cadlina laevis species complex produced with TCS method in PopART. Colours of circles refer to the geographic origin of each haplotype. The relative size of circles is proportional to the number of sequences of that same haplotype. РИС. 3. Сеть гаплотипов COI комплекса видов Cadlina laevis, полученнаЯ методом TCS в PopART. Цвета круЖков обоЗначают географическое происхоЖдение каЖдого гаплотипа. Относительный раЗмер круЖков пропорционален количеству последовательностей одного и того Же гаплотипа.

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FIG. 4 in Fifty shades of white: morphological and molecular diversity of the Cadlina laevis species complex (Gastropoda: Nudibranchia) in the North-West Pacific

FIG. 4. Photos of studied specimens from different localities, all measurements are indicated in preserved state. A. Cadlina laevis, MIMB47953, White Sea, 16 mm in length. B. Cadlina laevis, MIMB47970, Barents Sea, 13 mm in length. C. Cadlina laevis, MIMB47948, White Sea, 10 mm in length. D. Cadlina laevis, MIMB47965, White Sea, 9 mm in length. E. Cadlina laevis, MIMB47958, White Sea, 10 mm in length. F. Cadlina sp. 1, MIMB47971, Urup Is., Sea of Okhotsk, 22 mm in length. G. Cadlina sp. 2, MIMB42230, Iturup Is., Sea of Okhotsk, 18 mm in length. H. Cadlina sp. 3, MIMB47974, Sea of Japan, 10 mm in length. I. Cadlina sp. 3, MIMB47972, Sea of Japan, 15 mm in length. K. Cadlina sp. 5, MIMB47979, Iturup Is., Sea of Okhotsk, 11 mm in length. L. Cadlina sp. 7, MIMB47981, Sea of Japan, 25 mm in length. M. Cadlina sp. 6, MIMB47980, Shikotan Is., Sea of Okhotsk, 29 mm in length. N. Cadlina umiushi, MIMB48000, Sea of Japan, 17 mm in length. РИС. 4. Фотографии иЗученных ЭкЗемплЯров иЗ раЗных регионов, раЗмер тела укаЗан длЯ фиксированного состоЯниЯ. А. Cadlina laevis, MIMB47953, Белое море, длина 16 мм. B. Cadlina laevis, MIMB47970, Баренцево море, длина 13 мм. C. Cadlina laevis, MIMB47948, Белое море, длина 10 мм. D. Cadlina laevis, MIMB47965, Белое море, длина 9 мм. E. Cadlina laevis, MIMB47958, Белое море, длина 10 мм. F. Cadlina sp. 1, MIMB47971, о. Уруп, Охотское море, длина 22 мм. G. Cadlina sp. 2, MIMB42230, о. Итуруп, Охотское море, длина 18 мм. H. Cadlina sp. 3, MIMB47974, Японское море, длина 10 мм. I. Cadlina sp. 3, MIMB47972, Японское море, длина 15 мм. К. Cadlina sp. 5, MIMB47979, о. Итуруп, Охотское море, длина 11 мм. L. Cadlina sp. 7, MIMB47981, Японское море, длина 25 мм. М. Cadlina sp. 6, MIMB47980, о. Шикотан, Охотское море, длина 29 мм. N. Cadlina umiushi, MIMB48000, Японское море, длина 17 мм.

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