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opennotspecifiedMar 2020View details →

FIGURE 1 in Phaeosphaeriopsis omaniana (Phaeosphaeriaceae, Pleosporales), a novel fungus from Oman

FIGURE 1. MP tree resulting from analyzed LSU, ITS and TEF dataset. The scale bar indicates 30 changes. ML bootstrap support values> 50 % chains are shown at the nodes. Newly generated isolate is in orange bold text. The tree was rooted to Neostagonospora artemisiae MFLUC 17-0693 (Phaeosphaeriaceae).

FIGURE 2 in Morphology and phylogenetic analyses reveal Neopalmiascoma gen. nov. (Bambusicolaceae, Pleosporales) on Macadamia integrifolia in Yunnan Province, China

FIGURE 2. Neopalmiascoma macadamiae (ZHKU 23-0096, holotype). a. Appearance of ascomata on host substrate. b. Vertical section of an ascoma. c. Peridium. d. Hamathecium. e–g. Asci. h–k. Ascospores. l, m. Ascospore stained with Indian ink. n. A germinating ascospore. o, p. Colony on PDA from above and below after one month. Scale bars: c = 40 μm; b, d–g, n = 20 μm; j, k = 10 μm; h, i, l, m = 5 μm.

FIGURE 1 in Morphology and phylogenetic analyses reveal Neopalmiascoma gen. nov. (Bambusicolaceae, Pleosporales) on Macadamia integrifolia in Yunnan Province, China

FIGURE 1. RAxML tree based on a combined dataset of SSU, ITS, LSU, RPB2 and TEF1-α gene sequences. Bootstrap support values for maximum likelihood (ML) equal to or higher than 60% and Bayesian posterior probability (BYPP) equal to or higher than 0.90 are indicated above the branches. Newly generated sequences are shown in red, while the ex-type species are shown in bold black.

FIGURE 2 in Taxonomic classification of Praeclarispora herbicola comb. nov. in Leptosphaeriaceae (Pleosporales)

FIGURE 2 Praeclarispora herbicola (KUN-HKAS 124570). a, b. Ascomata on a dead stem of Bidens pilosa on the ground nearby a reservoir. c. Vertical section through ascoma with protruding ostiole. d, e. Section through peridia. f. Pseudoparaphyses. g, h. Asci (note: h = fissitunicate). i–n. Ascospores. Scale bars: a = 1 mm; b = 250 µm; c–e = 50 µm; f–n = 20 µm.

FIGURE 1 in Taxonomic classification of Praeclarispora herbicola comb. nov. in Leptosphaeriaceae (Pleosporales)

FIGURE 1 Phylogenetic tree generated from maximum likelihood analysis (RAxML) based on a combined LSU, SSU, and ITS sequence dataset. Maximum likelihood bootstrap values equal to or greater than 60% and Bayesian posterior probabilities equal to or greater than 0.90 PP are indicated at the nodes as MLBS/BYPP. Support values lower than 60% ML and 0.90 PP are indicated by "–". Ex-type strains are in bold, and the new strain is in blue.

opennotspecifiedJun 2020View details →

FIGURE 3 in Triseptata sexualis gen. et sp. nov. in Latoruaceae (Pleosporales)

FIGURE 3. Triseptata sexualis (MFLUCC 11–0002, ex-type). a Germinating ascospore. b, c Colonies on MEA from surface and reverse. d Aerial mycelium in culture. e, f Development of conidia in culture. g–i Close up of conidia–like structures. Scale bars: a = 5 µm, d = 10 µm, f = 10 µm, g–i = 20 µm.

opennotspecifiedJun 2020View details →

FIGURE 1 in Triseptata sexualis gen. et sp. nov. in Latoruaceae (Pleosporales)

FIGURE 1. Phylogram obtained from RAxML based on analysis of a combined dataset of LSU ITS and SSU sequence data. Bootstrap values for RAxML ≥ 55% BS and BYPP ≥ 0.95 are given on the branches. The tree is rooted to Dothidea insculpta CBS 189.58 (Dothideaceae, Dothideales). The type species of each genus are in bold and new taxa in blue.

opennotspecifiedJun 2020View details →

FIGURE 2 in Triseptata sexualis gen. et sp. nov. in Latoruaceae (Pleosporales)

FIGURE 2. Triseptata sexualis (MFLU 11–0002, holotype). a Appearance of ascomata and habit on dried branches substrate. b, c Section of ascomata. d Peridium. e Pseudoparaphyses. f–h Asci. i–l Ascospores. Scale bars: a = 500 µm, b, c = 100 µm, d, f-h = 20 µm, e, i–l = 5 µm.

opennotspecifiedMay 2024View details →

TABLE 2 in Multiple evidence reveals a new species of Neocucurbitaria (Cucurbitariaceae, Pleosporales) from China

TABLE 2. Synopsis of asexual morph of Neocucurbitaria species with the newly introduced species in bold.<table><tbody><tr><th>Taxa</th><th>Conidiomata</th><th>Conidiophores</th><th>Conidiogenous cells</th><th>Conidia</th><th>References</th></tr></tbody><tbody><tr><th>Neocucurbitaria acanthocladae</th><td>45–118 μm diam, mostly immersed in agar, solitary, irregularly disposed</td><td>-</td><td>4.5–7.5 × 2.2–4.9 μm, crowded, lageniform to subglobose, often with elongated neck</td><td>2.8–3.9 × 1.2–1.7 μm,,oblong to allantoid, with Jaklitsch et al. 0–2 subterminal guttules, (2018) smooth</td></tr><tr><th></th><td>38–147 μm diam, immersed to nearly superficial, densely</td><td></td><td></td><td>2.8–4.2 × 1.1–2.1 μm, oblong, straight or</td><td></td></tr><tr><th>N. aetnensis</th><td>but singly disposed or in small groups, more or less globose, olivaceous to nearly black, usually surrounded by brown, often submoniliform hyphae</td><td>-</td><td>3.2–7.7 × 1.9–5.7 μm, crowded, lageniform to globose</td><td>slightly curved, hyaline, partly becoming dilute brownish when aged, with 0–2, or more, subterminal guttules, smooth</td><td>Jaklitsch et al. (2018)</td></tr><tr><th>N. acerina.</th><td>43–141 μm diam, numerous, scattered to aggregated, globose to pulvinate, olivaceous, turning black within a week</td><td>-</td><td>4.0–9.5 × 1.6–5.3 μm, crowded, very variable, lageniform to subglobose</td><td>2.2–4.0 × 0.9–1.9μm, oblong to narrowly ellipsoid, straight or slightly curved, typically with 2 subterminal guttules, smooth</td><td>Jaklitsch et al. (2018)</td></tr><tr><th>N. cava</th><td>140–200 × 100–140 μm,, brown, solitary or confluent, semi-immersed or immersed, glabrous, subglobose, with one ostiolar neck</td><td>10–22 × 1.5–2.5 μm, smooth-walled, straight or sinuous to slightly curved, slightly tapering towards the apex, branched at the base</td><td>Integrated to the 2.5–3.5 × 1–1.5 μm, conidiophore, hyaline, aseptate, smooth- and smooth-walled, doliiform, thin-walled, mostly with a more or less cylindrical to slightly cylindrical collarette, up to allantoid, guttulate 3 per conidiophore</td><td>ValenzuelaLopez et al. (2018)</td></tr><tr><th></th><td>100–400 μm diam, pycnidial,</td><td>Integrated into the</td><td>3.1–4.2 ×1.7–3.0 um,</td><td></td></tr><tr><th>N. chlamydospora</th><td>olivaceous brown to almost black, globose or flask shaped, primarily solitary or confluent</td><td>smooth walled, straight to slightly curved, and branched</td><td>conidiophore, hyaline, smooth-walled, with a more or less cylindrical collarette</td><td>aseptate, oval to ellipsoid, Hong et al. straight or slightly (2023) curved, smooth, rounded at both ends</td></tr><tr><th>N. cinereae</th><td>54–141 μm diam, aggregated in groups of up to 10, turning dark olivaceous to nearly black, more or less globose, fusing laterally, papillate</td><td>-</td><td>3–7.8 × 2–4.6 μm, crowded, broadly conical, subglobose or lageniform</td><td>3–4.8 × 1.4–2.5 μm, oblong, sometimes attenuated toward one end, with 2 or more minute guttules, smooth</td><td>Jaklitsch et al. (2018)</td></tr><tr><th>N. cisticola</th><td>58–147 μm diam, scattered, more or less globose, hyaline, - turning olivaceous</td><td>4.5–10 × 1.8–6.3 μm, crowded, or solitary terminal, lageniform to subglobose</td><td>2.9–4.5 × 1.1–1.9 μm, oblong, straight or Jaklitsch et al. slightly curved, with (2018) 0–2 subterminal guttules, smooth</td></tr><tr><th>N. hakeae</th><td>50–90 µm diam, solitary, erumpent, globose, with central ostiole, with 15–25 µm diam central ostiole</td><td>7–25 × 2–3 µm, hyaline to pale brown, lining the inner cavity, branched, 1–3-septate</td><td>4–6 × 2.5–3.5 µm, terminal and intercalary, subcylindrical, hyaline, smooth to pale brown, with periclinal thickening</td><td>3–4 × 2 µm, solitary, smooth, guttulate, subcylindrical with obtuse ends</td><td>Crous et al. (2016)</td></tr><tr><th>N. irregularis</th><td>75–130 × 65–120 μm, brown, solitary or confluent, glabrous, subglobose to ovoid, with 3–4 papillate ostiole</td><td>-</td><td>2.5 × 3.5 μm, hyaline, smooth-walled, doliiform</td><td>2.5–4 × 1.5–2 μm, aseptate, smooth, thin-walled, ellipsoidal to cylindrical, guttulate</td><td>Valenzuela-Lopez et al. (2018)</td></tr><tr><th>N. juglandicola</th><td>53–142 μm diam, scattered below epidermis on subicular hyphae, also in association with ascomata, globose, sometimes with a blunt rounded apical papilla</td><td>-</td><td>4.3–7.2 × 1.4–2.5 μm, lageniform to cylindrical, 2.2–3.2 × 1.3–1.6 μm, formed on the hyaline oblong to ellipsoid, inner cells or terminally on smooth short, simple, 1–3 celled conidiophores</td><td>Jaklitsch et al. (2018)</td></tr><tr><th>N. keratinophila</th><td>180–200 × 130–150 μm, solitary, superficial, brown to dark brown, mostly glabrous or covered with somewhat short setae, globose to subglobose</td><td>With terminal and lateral openings</td><td>8–16 × 2–4 μm, ampulliform to doliform, integrated to acropleurogenous, branched at the base</td><td>3.5–4.5 × 1.5–3.5 μm, aseptate, smooth, thin-walled, subglobose to ellipsoid, with few guttules</td><td>ValenzuelaLopez et al. (2018)</td></tr><tr><th>N. populi</th><td>73–205 μm diam, partly immersed in agar, dark olivaceous to black, globose, longish, sometimes fusing, - with central ostiole, papillate or not, often covered by aerial hyphae</td><td>3.2–8.2 × 1.7–5 μm, crowded, lageniform to subglobose</td><td>2.8–4.8 × 1.1–1.9 μm, oblong to allantoid, with Jaklitsch et al. 0–2 subterminal guttules, (2018) smooth</td></tr><tr><th>N. quercina</th><td>70–90 μm diam, 100–230 × 90–130 μm when ovoid, brown, solitary or confluent, superficial, mostly glabrous or covered with somewhat shortest setae, globose, with 1–2 papillate ostiolar necks</td><td>6.5–14 × 2–3 μm, smooth-walled, straight or sinuous to slightly curved, slightly tapering towards apex, branched at base</td><td>Terminal and lateral, smooth-walled, ampulliform when terminal, with a more or less cylindrical collarette, up to 4 per conidiophore</td><td>1.5–3 × 1.2–1.5 μm, aseptate, smooth, thin-walled, ovoid to cylindrical, guttulate</td><td>Valenzuela-Lopez et al. (2018)</td></tr><tr><th>N. rhamni</th><td>50–157μm diam, scattered, accompanying ascomata, or erumpent in dense fascicles through bark, globose, sometimes collapsing becoming cupulate, with a small papilla, black, smooth and glabrous</td><td>-</td><td>Up to ca. 35 μm long, 2.5–3.5 × 1.3–2.1 μm, 1–3 celled, with solitary ellipsoid, oblong, often terminal phialides and attenuated toward one lateral pegs typically end, with 1–2 guttules, present at the upper end of smooth conidiophore cells</td><td>Jaklitsch et al. (2018)</td></tr><tr><th>N. rhamnicola</th><td>50–210 μm diam, more or less in radial rows, laterally fused, with hyaline to olivaceous conidial drops</td><td>-</td><td>2.5–4.3 × 1.1–2 μm, oblong or narrowly 4.0–8.7 × 2.0–6.0 μm, ellipsoid, straight or lageniform, often basally curved, also drop-like, inflated, arranged in dense often with 1–2 guttules, clusters sometimes scar distinctly truncate</td><td>Jaklitsch et al. (2018)</td></tr><tr><th>N. rhamnioides</th><td>43–141 μm diam, superficial on inner bark or wood on usually inconspicuous Bearing lateral pegs and subiculum, scattered among solitary phialides terminally ascomata, globose, often with a minute apical papilla</td><td>4.3–8.7 × 1.5–2.7, lageniform to cylindrical, straight or curved</td><td>2.5–3.8 × 1.0–1.5μm, oblong to allantoid, with 1–2 subterminal guttules</td><td>Jaklitsch et al. (2018)</td></tr><tr><th></th><td>ca. 70–100 μm diam, uncommon on host, scattered</td><td></td><td></td><td></td><td></td></tr><tr><th></th><td>among ascomata, globose, with a minute apical papilla, black, more or less smooth</td><td>-</td><td></td><td></td><td></td></tr><tr><th>N. ribicola</th><td>33–70 μm diam, numerous, globose, with large light rounded papilla 12–30 μm diam, pale olivaceous, darkening, scattered or densely</td><td>-</td><td>5.7–9.2 × 2.1–3.3 μm, short, 1–3-celled</td><td>2.6–5 × 1.2–2.4 μm, oblong to ellipsoid Jaklitsch et al. or drop-like, with (2018) 1–2 subterminal guttules, smooth</td></tr><tr><th></th><td>128–147 × 96–110 μm, semi-immersed from bark,</td><td></td><td></td><td></td><td></td></tr><tr><th>N. subribicola</th><td>hermetically aggregated on subiculum in valsoid groups, pycnidia, globular to subglobular, coriaceous, without distinguishable ostioles</td><td>-</td><td>8–15 × 1–3 μm, enteroblastic, solitary, cylindrical, smooth</td><td>3–5 × 1–2 μm, oval to spherical, hyaline This study aseptate, smooth, with a minute guttule</td></tr><tr><th>N. unguis-hominis</th><td>100–200 µm diam, numerous, solitary to aggregated, 20–40 × 2–2.5 µm, simple or dark brown, subglobose to branched, septate, cylindrical globose, unilocules.</td><td>phialidic, enteroblastic, simple, cylindrical</td><td>2–3 × 1 µm, cylindrical, straight or curved, with guttule</td><td>Punithalingam & English (1975)</td></tr><tr><th>N. vachelliae</th><td>53–195 μm diam, numerous, partly immersed in agar, scattered to aggregated, globose, with central papilla, sometimes laterally fused, pale olive, becoming dark olivaceous to black</td><td>simple or basally divided into several nearly parallel branches with lateral pegs and solitary terminal phialides</td><td>-</td><td>2.6–5.1 × 1.2–2.1 μm, oblong to allantoid, Jaklitsch et al. sometimes pinched, with (2018) 0–2 subterminal guttules, smooth</td></tr></tbody></table>......continued on the next page......continued on the next page......continued on the next page

FIGURE 4 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)

FIGURE 4. Sexual stage of Shiraia bambusicola (epitype). (Figs A–D, F–M, O–P light microscopy and Figs E, N SEM). A. Ascostroma with yellow ascospore mass. B. Cross section of ascostroma. Note the sexual locules lining the periphery (a) and the asexual locules near the centre (b). The outer part is pink, while the inner part is white to light pink. C. Cross section of locules (fluorescent microscopy stained in Hoechst 33258). D. Vertical section of ascostroma. E. Tissue of locule wall. Note the cells of textura angularis at the inside fusing with cells of textura intricata at the outside. F. Pseudoparaphyses (cotton blue reagent staining). G. Asci and pseudoparaphyses. H–K. Asci. L–M. Apices of asci showing ocular chamber. N–P. Ascospores (P stained in lactic acid phenol Medan). Scale bars: A = 1 cm; B = 1.5 cm; C–D = 100 µm; E = 10 µm; F = 20 µm; G–K = 50 µm; L–M = 20 µm; N–P = 10 µm.

FIGURE 3 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)

FIGURE 3. Asexual state of Shiraia bambusicola (epitype). (Figs A–B, D–E, J–N light microscopy and Figs C, F–I SEM) A. Ascostroma on bamboo branches. Note that the ascostromata form at the end of branches, but below the leaf petioles. B. Ascostromata with yellow conidium mass. C. Cross section of ascostroma. Note the leaf sheath of the host bamboo with green drawing and asexual locule (arrowed). D–E. Asexual locule (D shows release of the conidia, E fluorescent microscopy stained in Hoechst 33258). F–G. Conidia growing on the wall of the conidiomata. H. Cells of the asexual locule. I. Conidium on short conidiophore. J–L. Conidia. M– N. Colony (face and reverse views) on PDA after 14 days at 25°C. Scale bars: A = 2 cm; B = 1 cm; C = 1 mm; D = 200 µm; E = 300 µm; F–G =20 µm; H–L = 10 µm.

FIGURE 1 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)

FIGURE 1. Phylogram constructed using MP analysis of 28S nrDNA nucleotide sequences. Bootstrap support values>50% are shown above the branch. Dothidea sambuci is the outgroup taxon. The original isolate numbers are noted after the species names.

FIGURE 2 in Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota)

FIGURE 2. Shiraia bambusicola (holotype) A. Surface of stroma. B–C. Section of stroma in cotton blue reagent showing arrangement of locules. D. Section of locule wall. E–F. Six-spored asci and pseudoparaphyses. G. Ascus. H. Conidium. I. Muriform ascospore. Scale bars: C = 200 µm, D–I = 40 µm.

opennotspecifiedDec 2013View details →

FIGURE 1 in Halojulellaceae a new family of the order Pleosporales

FIGURE 1. RAxML tree based on a combined dataset of SSU, LSU, RPB2 and TEF1, bootstrap support values for maximum likelihood (Black), maximum parsimony (Blue) greater than 50% and bayesian posterior probabilities greater than 0.90 (Red) below and above the nodes. Dothidea sambuci is the out group taxon. The original isolate numbers are noted after the species names.

opennotspecifiedDec 2013View details →

FIGURE 3 in Halojulellaceae a new family of the order Pleosporales

FIGURE 3. Julella buxi (holotype). A–C. Surface view of ascomata on host substrate (bark). D–E. Longitudinal section ascoma with clypeus. F. Close up of the peridium. g. Broad, septate pseudoparaphyses. H–J. Two spored asci lacking an ocular chamber. K–L. Muriform ascospores. M. Ascospore mounted in Indian ink to show mucilaginous sheath. Scale bars: D–E = 80µm, F = 10 µm, H–J = 25µm, K–M = 15 µm.

opennotspecifiedDec 2013View details →

FIGURE 2 in Halojulellaceae a new family of the order Pleosporales

FIGURE 2. Halojulella avicenniae (Material examined: BBH 23394); A: Surface view of ascomata on twigs of Avicennia wood. B: Close up of the ascomata. C: Section through mature ascoma. D. Close up of the peridium E. Cellular, septate pseudoparaphyses. F–G: 8-spored asci with an ocular chamber surrounded by distinct apical apparatus H: Apex of ascus, showing distinct apical apparatus. I– K. Pale brown matured ascospore. J–K Ascospores mounted in Cotton Blue to show mucilaginous sheath. Scale bars: A–B = 200 µm; C = 150 µm E–F = 40 µm; F–G = 80 µm, H = 30µm; I–L = 10 µm.

FIGURE 1 in Macrodiplodiopsis in Lophiostomataceae, Pleosporales

FIGURE 1. RAxML tree based on a combined dataset of LSU, SSU and EF1-α sequences. Bootstrap support values for maximum likelihood (ML) and maximum parsimony (MP) greater than 50% are given above the nodes; Bayesian posterior probabilities are given below the nodes. The original culture numbers are given after the species names. The tree is rooted to Massaria anomia (CBS 591.78). All sequences from type strains are in bold.

FIGURE 3 in Macrodiplodiopsis in Lophiostomataceae, Pleosporales

FIGURE 3. Macrodiplodiopsis desmazieri (MFLU 13-0090) A−D. Different stages of conidiogenesis. E−H. Mature conidia with mucilaginous sheath. Scale bars: A, B, D = 15 µm, C, E−H = 30 µm.

FIGURE 2 in Macrodiplodiopsis in Lophiostomataceae, Pleosporales

FIGURE 2. Macrodiplodiopsis desmazieri (MFLU 13-0090) A. Conidiomata on the host. B−C. Cross section of conidiomata. D−E. Conidiomata wall. F. Short neck of the conidiomata. G−I. Different stages of conidium development. J. Conidia. Scale bars: B, C=200 µm, D, E, I, J = 40 µm, F = 100 µm, G, H = 30 µm.

FIGURE 2 in Freshwater ascomycetes: Lophiostoma vaginatispora comb. nov. (Dothideomycetes, Pleosporales, Lophiostomaceae) based on morphological and molecular data

FIGURE 2. Lophiostoma vaginatispora (from holotype). A–B. Appearance of ascomata on wood. Note the oozed ascospores in B. C–D. Sections of ascomata. E. Papillate ascoma. F. Peridium. G. Wide cellular pseudoparaphyses. H–I. Asci. J–K. Asci releasing ascospores through fissitunicate dehiscence. L–N. Ascospores with wide papilionaceous sheath. L is in Indian ink. Scale bars: C = 300 µm, D = 200 µm, E = 50 µm, F–G = 20 µm, H–I = 30 µm, J–L, N = 50 µm, M = 20 µm.