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106 results for “Sorghum bicolor”
Fig. 1 in Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent
Fig. 1. Formation of NADES derived from natural occurring metabolites in plants. A) Chemical structures of D-glucose, tartaric acid, malic acid, choline, glycerol and dhurrin. Mixtures of these metabolites were tested for their ability to form NADES and their potential role in stabilizing the dhurrin biosynthetic enzymes. B) Stoichiometric mixture of glucose and tartrate constitute a NADES with significantly lowered melting point compared to the individual components. C) Biosynthetic pathway of the natural product dhurrin in S. bicolor.
Fig. 7 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo
Fig. 7. Flow cytometer analysis of LB accumulation in yeast cells. (A) Cytogram of BODIPY fluorescence vs. side scatter of cells expressing forage sorghum SbDGAT1- 1 variants. (B) Mean intensity of P3/fluorescent population (represents BODIPY uptake) events transformed with different variants of forage sorghum SbDGAT1-1 genes. The error bars represent the SD of three biological replicates. Asterisks indicates significant differences according to student t-test results where * = p <0.05.
Fig. 4 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo
Fig. 4. Transcript expression patterns of SbDGAT1-1 and SbDGAT1-2 in (A) bran (B) embryo (C) endosperm of forage sorghum grains. Bar diagram showed the relative expression pattern of SbDGAT1-1 and SbDGAT1-2 genes normalized against serine/threonine-protein phosphatase (PP2A-1, Accession no.: XM_002453490) as the reference gene by qRT-PCR. 10 DAP, 15 DAP, 20 DAP, 25 DAP and 30 DAP represents 10, 15, 20, 25 and 30 days after pollination of forage sorghum grain development. The bars were standard deviations (SD) of three technical replicates prepared from pooled tissues. (D) The SbDGAT1-1 and SbDGAT1-2 tissue-specific expression (embryo, endosperm, seed 5 DAP and seed 10 DAP) patterns were identified using the EMBL-EBI expression atlas database. FPKM; Fragments Per Kilobase of transcript per Million mapped reads.
Fig. 5 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo
Fig. 5. Overexpression of SbDGAT1 genes and its variants in yeast TAG mutant H1246. (A) Schematic representation of the N-terminal region of SbDGAT1 genes. Red arrow indicates the position of truncation in SbDGAT1-1. (B) TAG mutant H1246 strain transformed with SbDGAT1 full-length and truncated variants restores the TAG biosynthesis capability compared with control as visualized in TLC. The spots of triacylglycerol (TAG), diacylglycerol (DAG) and free fatty acids (FFA) are separated in TLC plate (C) Total TAG content of yeast H1246 cells expressed with full-length (SbDGAT1-1 and SbDGAT1-2) and truncated variants (SbDGAT1-1(39-515) and SbDGAT1-1(89-515)). Wild type yeast cells (BY4741) were used as positive control. (D) FA profile of TAG products isolated from yeast H1246 strain expressed with full-length and truncated variants of SbDGAT1 genes. Saturated FA abbreviations are as follows: 10:0, Capric acid; 12:0, Lauric acid; 14:0, Myristic acid; 15:0, Pentadecylic acid; 16:0, Palmitic acid; 18:0, Stearic acid. Mono-unsaturated FA abbreviations are as follows: 14:1, Myristoleic acid; 16:1, Palmitoleic acid; 18:1, Oleic acid. The error bars represent the SD of three biological replicates. Asterisks indicates significant differences according to student t-test results where * = p <0.05, ** = p <0.01. H1246 transformed with the empty pYES2 vector served as negative control and wild type yeast (BY4741) served as a positive control. FA, fatty acid; DW, dry weight. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 3 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo
Fig. 3. DISOPRED plot representing the intrinsically disordered regions in (A) SbDGAT1-1 (B) SbDGAT1-2 protein.
Fig. 6 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo
Fig. 6. LB observation of yeast H1246 transformants with SbDGAT1 variants using confocal microscopy. H1246 mutant strains (transformed with the empty pYES2 vector) and SbDGAT1 variants were observed under confocal microscope. Wild type yeast cells (BY4741) were used as positive control and H1246 as negative control. BODIPY493/503 dyes were used to visualize the yeast LBs.
Fig. 2 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo
Fig. 2. Sequence alignment of SbDGAT1-1 (XP_002439419) and SbDGAT1-2 (XP_021304830) deduced amino acid sequences with biochemically characterized AtDGAT1 (CAB45373). Putative N-terminal region is highlighted with blue colour. Conserved sequence domains are boxed, the acyl-CoA binding site (Jako et al., 2001; Ayme et al., 2015), active site (Jako et al., 2001), typical SnRK1 protein kinase binding motif (Zou et al., 1999; Xu et al., 2008), thiolase acyl-enzyme intermediate binding motif (Zou et al., 1999; Xu et al., 2008), FA binding protein signature (Guo et al., 2017), DAG-binding site (Guo et al., 2017) and ER retrieval motif (Aznar-Moreno et al., 2015). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 in Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo
Fig. 1. Phylogenetic relationship between SbDGAT1-1 and SbDGAT1-2 with other DGAT1 and DGAT2 protein sequences. Phylogenetic tree was constructed using maximum likelihood method (Tamura et al., 2011; Hall, 2013) in Molecular Evolutionary Genetics Analysis (MEGA) 7.0 tool (Kumar et al., 2016). Bootstrap values are mentioned in the branches. GenBank accession numbers are indicated with species names of the DGAT proteins.
The Efficacy of Jobelyn (Sorghum Bicolor Extract)in the Treatment of Sickle Cell Anemia
ClinicalTrials.gov study NCT01703104. IPD Sharing: Not stated. Countries: 1. Publications: 12.
Quality of Life Study for Sickle Cell Patients Treated With Jobelyn (Sorghum Bicolor Extract)
ClinicalTrials.gov study NCT01704794. IPD Sharing: Not stated. Countries: 1. Publications: 12.
Antipsychotic Effects of Sorghum Bicolor (JOBELYN) in the Treatment of Schizophrenia
ClinicalTrials.gov study NCT02240173. IPD Sharing: Not stated. Countries: 1. Publications: 8.
Data from: Surveying the spatial distribution of feral sorghum (Sorghum bicolor L.) and its sympatry with johnsongrass (S. halepense) in South Texas
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Data from: Accelerating seed germination and seedling development of Sorghum (Sorghum bicolor L. Moench) through hydro-priming
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Data from: Seed priming and Zaï pit practice improve field performance of sorghum (Sorghum bicolor L. Moench) in the Sahelian zone of Mali
Poor seed germination caused by a variety of seed, soil and environmental factors lead to suboptimal seedling stand and ultimately yield suffers a serious setback. A number of priming techniques have been found to be effective in increasing germination and seedling growth and development. This includes techniques such as on-farm hydro-priming (seed soaking in well water) and osmo-priming (seed soaking in low osmotic solutions of Potassium silicate). Also, Zaï practice, a cropping system concentrating runoff water and manure in pits, may be a simple solution in addition to seed priming techniques for crop establishment under early drought and heat prone areas and for increased productivity of poor soils. This study aims to evaluate the combined effect of priming and cultural practices (ridge and zaï pits) on three sorghum varieties in the Sahelian zone of Mali. Data were collected during two growing seasons in 2014 and 2015. Monitored parameters were assessed by comparing the impact of priming with untreated sorghum seeds (the control) cultivated on ridges and zaï pits with or without compost. We also compared the effect of hydro-priming in water from well to osmo-priming using K2SiO3 performance. Hence, each of the 3 sorghum variety seeds were subjected to 3 different priming, sowed on ridges and zaï treatments. Variety banidoka and CSM63E recorded the maximum GP (54.0%) which was significantly higher than saba-tienda GP (48%). The priming treatments were not statistically different for germination but showed slight improvement in GP for primed (54%) compared to unprimed (48%). Germination percentages were significantly higher in zaï pits practice without (55%) and with compost (55%) compared to ridges (48%). Grain yield varied significantly among sorghum varieties (p<0. 04) and cultural practices (p<0. 01). There was no significant difference among priming treatments for grain yield. However, variety banidoka produced the highest grain yield (961 kg ha-1) followed by CSM63E (874 kg ha-1) and lastly saba-tienda (671 kg ha-1) with banidoka showing statistically different yield compared to saba-tienda. Zaï pits practice with compost obtained the highest GY (1131 kg ha-1), which was statistically different from the GY of zaï pits practice without compost (695 kg ha-1) and ridge practice (742 kg ha-1). Zaï pits practice without compost did not statistically differ from ridge practice. The findings showed that seed priming in addition to zaï pits practice improves seed germination and seedling development of sorghum probably by accelerating imbibition and providing continuous moisture during the germination phase. Combining seed priming and zai pit practice with compost appears a simple practice that will improve crop establishment and grain yield in the Sahelian zone of West Africa.
Data from: Population genomics of sorghum (Sorghum bicolor) across diverse agroclimatic zones of Niger
Improving adaptation of staple crops in developing countries is important to ensure food security. In the West African country of Niger, the staple crop sorghum (Sorghum bicolor) is cultivated across diverse agroclimatic zones, but the genetic basis of local adaptation has not been described. The objectives of this study were to characterize the genomic diversity of sorghum from Niger and to identify genomic regions conferring local adaptation to agroclimatic zones and farmer preferences. We analyzed 516 Nigerien accessions for which local variety name, botanical race, and geographic origin were known. We discovered 144,299 single nucleotide polymorphisms (SNPs) using genotyping-by-sequencing (GBS). We performed discriminant analysis of principal components (DAPC), which identified six genetic groups, and performed a genome scan for loci with high discriminant loadings. The highest discriminant coefficients were on chromosome 9, near the putative ortholog of maize flowering time adaptation gene Vgt1. Next, we characterized differentiation among local varieties and used a genome scan of pairwise FST values to identify SNPs associated with specific local varieties. Comparison of varieties named for light- versus dark-grain identified differentiation near Tannin1, the major gene responsible for grain tannins. These findings could facilitate genomics-assisted breeding of locally-adapted and farmer-preferred sorghum varieties for Niger.
Phenotypic data for the Sorghum bicolor core collection selected from the whole collection in the Uganda National GeneBank
<p>Passport data for the Sorghum bicolor core collection selected from the whole collection in the Uganda National GeneBank (Information is based on 286 core collection accessions, excluding 24 accessions which did not germinate in this trial</p>
Construction of an Ultra-High-Density Consensus Linkage Map in Sorghum bicolor
<p>A consensus genetic map were created by merging linkage maps from four published studies (Ji et al. 2017; Lopez et al. 2017; Kong et al. 2018; Phuong et al. 2019) using package LPmerge of R software.</p>
Sorghum bicolor (L.) Moench (BR0000011615231)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Sorghum bicolor (L.) Moench (BR0000011615743)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Sorghum bicolor (L.) Moench (BR0000012354283)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
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