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76
datasets available to search
ShareScore release 0.9.0
Dataset results
76 results for “anticancer drug”
Drug-induced change in gene expression across NCI-60 cell lines after exposure to 15 anticancer agents for 2, 6 and 24h (topotecan)
GEO Series GSE116450. Homo sapiens. 525 samples. Type: Expression profiling by array.
Exosomal SNHG26 derived from drug-resistant tongue squamous cell carcinoma cells inhibits the anticancer effect of NK cells in the microenvironment
GEO Series GSE298791. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Drug-induced change in gene expression across NCI-60 cell lines after exposure to 15 anticancer agents for 2, 6 and 24h (vorinostat)
GEO Series GSE116451. Homo sapiens. 528 samples. Type: Expression profiling by array.
Anticancer properties of distinct antimalaria drug classes
GEO Series GSE51739. Homo sapiens. 91 samples. Type: Expression profiling by array.
Drug-induced change in gene expression across NCI-60 cell lines after exposure to 15 anticancer agents for 2, 6 and 24h (dasatinib)
GEO Series GSE116440. Homo sapiens. 496 samples. Type: Expression profiling by array.
Drug-induced change in gene expression across NCI-60 cell lines after exposure to 15 anticancer agents for 2, 6 and 24h (geldanamycin)
GEO Series GSE116443. Homo sapiens. 496 samples. Type: Expression profiling by array.
Drug-induced change in gene expression across NCI-60 cell lines after exposure to 15 anticancer agents for 2, 6 and 24h (doxorubicin)
GEO Series GSE116441. Homo sapiens. 516 samples. Type: Expression profiling by array.
Anticancer drug clustering in lung cancer based on gene expression profiles and sensitivity database
GEO Series GSE4127. Homo sapiens. 29 samples. Type: Expression profiling by array.
Patient-derived metastatic melanoma cells treated with vitamin D and anticancer drugs
<ol> <li><strong>Immunofluorescence staining</strong></li> </ol> <p>For immunofluorescence analyses cells isolated from malignant melanoma tumour were seeded in 8-well chamber slides, fixed with 4% paraformaldehyde (PFA) for 10 min and permeabilized for 5 min in 0.2% Triton X100 in PBS. Slides were washed three times with PBS and incubated with 1% BSA for 30 min at RT. Following blocking appropriate primary antibody in 0.1% BSA (Thermo Fisher Scientific: PMEL (HMB45) MA5-13232, 1:40; Melan-A MA5-15237, 1:100 or MA5-32217, 1:200; Cell Signaling Technology: FAP Cat. No. 66562, 1:100) was applied and the slides were incubated in humidified chamber at 4 °C overnight. Subsequently slides were washed three times in PBS, and incubated with secondary antibody (ThermoFisher Scientific either Alexa Fluor® 488 conjugate goat anti rabbit IgG A11008 or Alexa Fluor® 594 conjugate donkey anti mouse IgG A21203) solution in PBS for 1 h at RT in dark. Following three washing steps in PBS the slides were counterstained with 4′,6′-diamidinio-2-phenylindole (DAPI). Images were collected with a fluorescence microscope Olympus cell Vivo IX 83. For a-SMA (alpha-smooth muscle actin) immunofluorescence analyses the procedure of staining with Melan A was proceeded to the appropriate secondary antibodies as described above. Subsequently antibody against a-SMA PE‑conjugated (R&D Systems: IC1420P, 1:50) was applied to the specimen for an additional 30 min in humidified chamber at 4 °C. Following three washing steps in PBS the slides were counterstained with DAPI. Images were collected with a fluorescence microscope Olympus cell Vivo IX 83 as Z‑stacks.</p> <ol> <li><strong>SRB viability assay</strong></li> </ol> <p>The viability of cells isolated from metastatic malignant melanoma tissues treated with 1,25(OH)<sub>2</sub>D<sub>3</sub>, vemurafenib or both was assessed with sulforhodamine B (SRB). The cells were seeded in 96-well plates at 2,000 of cells per well density and left overnight. Subsequently the cells were treated simultaneously with serial dilutions of vemurafenib (3,15-200 nM) and 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM or 1 µM concentration for 72 h. Cells were fixed with 10% trichloroacetic acid for 1 h at 4°C, washed with distilled water and stained for 15 min with 0.4% SRB solution (Sigma–Aldrich; Merck KGaA) in 1 % acetic acid. SRB dye was solubilized using a solution of 10 mM buffered Tris Base (pH 10.5) and absorbance measured at 570 nm using an Epoch spectrophotometer (BioTek, Winooski, USA). The relative IC50 value was calculated as the mid‑point between no inhibition and the maximum observed decrease in proliferation (n=6).</p> <ol> <li><strong>Proliferation rate assay</strong></li> </ol> <p>Proliferation rate analysis of the cells isolated from malignant melanoma tissues treated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration and cediranib at 1000 nM concentration was carried out as a live imaging with Olympus cell Vivo IX 83, equipped with CO<sub>2</sub> incubator and enables for stable cell cultivation at 37°C, controlled humidity and 5% CO<sub>2</sub> during the whole 72 h experiment. Results were calculated with the Olympus cell Vivo IX 83 software with use of TruAI technology, normalized with 1.0 at the beginning of the experiment (n = 4).</p> <ol> <li><strong>Wound closure rate</strong></li> </ol> <p>The experiment was carried out as a live imaging with Olympus cell Vivo IX 83 and cell migration process was observed during 72 h incubation. The cells isolated from malignant melanoma tissues were seeded on a 8-well chamber slide (1.0 × 10<sup>5</sup> cells per well). A mechanical wound was created by scraping the confluent monolayer of cells with a 200 µL pipette tip. Cediranib at 1000 nM concentration was added to the cells pretreated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration for 24 h. The cell free area was calculated as a percentage closure relative to original size [(wound area in mm<sup>2</sup>)*100/(original wound area in mm<sup>2</sup>)] with use of TruAI technology of the Olympus cellSens software (n = 4).</p> <ol> <li><strong>Mitochondrial membrane potential</strong></li> </ol> <p>Patients-derived cells were seeded on an 8-well chamber slide (2.0 × 10<sup>4</sup> cells per well). After 24 h, cells were stained with MitoTracker™ Red CM-H<sub>2</sub>Xros (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 70 nM concentration for 20 min at 37°C, and washed two times with PBS. Cells were pretreated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration for 24h. Subsequently cediranib at 1000 nM concentration was added to the cells in a fresh medium and mitochondria were observed for additional 24 h. The experiment was carried out as a live imaging with Olympus cell Vivo IX 83. Results were calculated as an area fraction ROI (“the mitochondria”) normalized with 1.0 at the beginning of the experiment with the Olympus cell Vivo IX 83 software, (n = 3 - 4).</p> <ol> <li><strong>Measurement of melanoma bioenergetics</strong></li> </ol> <p>Patients-derived cells were seeded at a density of 20,000 cells per well in an Agilent Seahorse microplate and cultured at 37°C in 5% CO<sub>2</sub> until reaching 80% confluency in 80 μL culture medium per well. Subsequently cells were incubated for 24 h with vemurafenib at 200 nM concentration or cediranib at 1000 nM concentration with or without 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration. Additionally, selected cells were also pretreated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration for 24 h and then incubated with the chosen anticancer drug at appropriate concentration for 24h. The Cell Mito Stress Test was performed as described by Franczak M. et al. (Franczak, M.; Kutryb-Zajac, B.; El Hassouni, B.; Giovannetti, E.; Granchi, C.; Minutolo, F.; Smolenski, R.T.; Peters, G.J. The effect of lactate dehydrogenase-A inhibition on intracellular nucleotides and mitochondrial respiration in pancreatic cancer cells. <em>Nucleosides Nucleotides Nucleic Acids </em><strong>2022</strong>, 1-11, doi:10.1080/15257770.2022.2031215.). The medium was changed before analysis for the XFp assay medium with 10 mM glucose, 2 mM glutamine and 1 mM pyruvate (Agilent, US). During the assay 1.5 μM oligomycin, 1 μM carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) and 0.5 μM rotenone with antimycin were added sequentially for the Cell Mito Stress Test. The XFp sensor cartridge, with compounds used in the test, was incubated at 37 °C in the non-CO<sub>2</sub> incubator for 15 minutes before the assay and plate with cells for 45 minutes. Each analysis was performed by the Agilent Seahorse XFp Analyzer (Agilent, Santa Clara, CA, USA). The oxygen consumption rate (OCR) and the mitochondrial respiration parameters were calculated with the software provided with the Agilent Seahorse XFp Analyzer (n = 2-5).</p>
Emergence of Bacterial Resistance to Antibiotics in the Digestive Microbiota of Patients Treated With Anticancer Drugs
ClinicalTrials.gov study NCT07093593. IPD Sharing: NO. Countries: 0. Publications: 0.
Cancer Therapy-related Cardiac Dysfunction Occuring in Children Treated by Anticancer Drugs for Malignancies
ClinicalTrials.gov study NCT05602103. IPD Sharing: NO. Countries: 0. Publications: 0.
Drug-induced change in gene expression across NCI-60 cell lines after exposure to 15 anticancer agents for 2, 6 and 24h (cisplatin)
GEO Series GSE116439. Homo sapiens. 499 samples. Type: Expression profiling by array.
Drug-induced change in gene expression across NCI-60 cell lines after exposure to 15 anticancer agents for 2, 6 and 24h (sunitinib)
GEO Series GSE116449. Homo sapiens. 513 samples. Type: Expression profiling by array.
In vitro throughput screening of anticancer drugs by patient-derived cell lines cultured on vascularized three-dimensional stromal tissues
GEO Series GSE248026. Homo sapiens. 2 samples. Type: Expression profiling by array.
Toxicogenomic responses of low level anticancer drug exposures in Daphnia magna
GEO Series GSE115386. Daphnia magna. 15 samples. Type: Expression profiling by high throughput sequencing.
miR-27b synergizes with anticancer drugs
GEO Series GSE53872. Homo sapiens. 2 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.