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opencc-by-4.0Apr 2020View details →

Fig. 2 in Comparison of in vitro methods to inhibit growth of a virulent strain of Batrachochytrium dendrobatidis (Longcore, Pessier, and Nichols 1999)

Fig. 2. (A) and (B) Duplicate Bd negative controls, (C) E. coli-JP1000 (left bacterial streak) and Bacillus sp. (right bacterial streak), (D) E. coli-JP1000 displaying a clear ZOI surrounding the bacterial streak, (E) E. coli control.

Figure 6 in Human induced trauma and directed take inhibits sea turtle recovery in the Commonwealth of the Northern Mariana Islands

Figure 6. Locations of opportunistic sea turtle stranding recoveries on Tinian, CNMI from July 2009 to July 2016.

Figure 4 in Human induced trauma and directed take inhibits sea turtle recovery in the Commonwealth of the Northern Mariana Islands

Figure 4. Examples of trauma, predation, impact, and entanglement in CNMI: A) butchered green turtle carapace and head; B) spear piercing neck of live juvenile green turtle; C) three-prong spear impact to cranium; D) large stainless steel hook embedded in ventral neck; E) rubber (inner tube) straps binding front flippers; F) nesting female immobilized by flipping onto carapace; G) hatchling retained as pet; H) shark predation; I) marine debris entanglement (note marked disfigurement due to debris); and J) boat strike (photo depicts carapace repair-arrow). Photos taken under USFWS recovery permit no. TE017352-17.

Figure 5 in Human induced trauma and directed take inhibits sea turtle recovery in the Commonwealth of the Northern Mariana Islands

Figure 5. Locations of sea turtle stranding recoveries on Saipan, CNMI from April 2005 to September 2016.

Figure 3 in Human induced trauma and directed take inhibits sea turtle recovery in the Commonwealth of the Northern Mariana Islands

Figure 3. Primary causes of injury and mortality of dead and live stranded sea turtles in Saipan and Tinian from 2005-2016 (n = 89). Trauma includes butchery, spear/bullet damage, rubber inner tube binding & improper husbandry. Nutritional refers to emaciation, predation is from sharks, entanglement sources include marine debris, lines and nets. Infectious damage is evidenced by inflammation.

Figure 1 in Human induced trauma and directed take inhibits sea turtle recovery in the Commonwealth of the Northern Mariana Islands

Figure 1. Map of the Mariana Islands Archipelago showing the study site locations (denoted by stars) of the islands of Saipan and Tinian, CNMI.

opencc-by-4.0Apr 2019View details →

Fig. 1 in Ability of sterile males to inhibit female remating in the melon fly Zeugodacus cucurbitae (Diptera: Tephritidae)

Fig. 1. Numbers of rematings observed per cage for females first mated to wild or sterile males at 3 intervals afer the initial mating. Each cage held 10 test females. Symbols represent mean values ± 1 SE; N = 8 in all cases.

opencc-by-4.0Sep 2024View details →

Data accompanying "HSP70 inhibits CHIP E3 ligase activity to maintain germline function in Caenorhabditis elegans" article.

This work was funded by the National Science Centre, Poland (grant PRELUDIUM number 2021/41/N/NZ1/03086) (to P.T.) and by the Deutsche Forschungsgemeinschaft (DFG; German Research Foundation) under Germany’s Excellence Strategy – EXC 2030 – 390661388 and – FOR 5504 – project number 496650118 (to T.H.). M.T.P. received support by the Cologne Graduate School of Aging Research. N.A.S., A.S., K.J., and M.N. were supported by the International Institute of Molecular and Cell Biology in Warsaw.

opencc-by-4.0Jan 2024View details →

β-Carotene alleviates substrate inhibition of a transferase caused by asymmetric cooperativity

Initial topology, parameter and coordinates files of the Molecular dynamics (MD) simulations of NbUGT72AY1. Five systems were simulated and analysed: NbUGT72AY1 (PDB 9J9K, complex V): <ul> <li>system 1: structure APO</li> <li>system 2: structure in complex with scopoletin </li> <li>system 3: structure in complex with UDPG</li> <li>system 4: structure in complex with UDPG and scopoletin</li> </ul> We used ACEMD3 (v3.7.1) as the molecular engine and AMBER as the force field. The three replicas of 1 µs (xtc files) were concatenated in a single trajectory for each system. Water molecules and ions atoms were remove from the original trajectories and topology before the upload (dry_trj.pdb).

opencc-by-4.0Jul 2024View details →

Hydroxyurea inhibits proliferation and stimulates apoptosis through inducible nitric oxide synthase in erythroid cells.

<a name="_Hlk164856369"></a>Hydroxyurea (HU) arrests cells in S-phase through the inhibition of ribonucleotide reductase and DNA synthesis, which is contributed significantly by the release of nitric oxide (NO). We investigated the mediation of inducible NO synthase (NOS2) in HU cytostatic effects, <a name="_Hlk165036602"></a>using in vitro shRNA-induced knock-down of NOS2 or specific NOS2 inhibitor (1400W), and supported by Nos2 knockout mice (Nos2-/-) to observe ex vivo murine erythroid progenitors (mERP).<a name="_Hlk165036694"></a>As a long-term effect, HU increased NOS2 expression in human erythroleukemic HEL92.1.7 cells (via NFκB signaling) and mERP. As a short-term result, HU increased the activity of purified and HEL92.1.7 cell-derived NOS2, as confirmed by NO / citrulline production and in silico molecular docking via hydrogen bonds. Molecular dynamic simulation showed a slight decrease in NOS2 receptor rigidity upon HU binding, confirming the stability of HU at the active site of the NOS2 receptor. Both 1400W and shRNA prevented the in vitro reduction of proliferation and induction of apoptosis of HEL92.1.7 cells by HU. Thus, shRNA blocked preferentially early apoptosis and only shRNA overcame HU-induced S-phase arrest of HEL92.1.7 cells. Both 1400W and Nos2 knockout prevented the in vivo decrease in proliferation and induction of apoptosis of mERP by HU from wild-type and Nos2-/- mice, respectively. Therefore, HU increased NOS2 activity and expression via NFκB signaling. This study demonstrated the NOS2 dependance in the HU inhibition of proliferation and stimulation of apoptosis of erythroid cells.

opencc-zeroJul 2021View details →

Cdt1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis

A fundamental concept in eukaryotic DNA replication is the temporal separation of G1 origin licensing from S phase origin firing. Re-replication and genome instability ensue if licensing occurs after DNA synthesis has started. In humans and other vertebrates, the E3 ubiquitin ligase CRL4Cdt2 starts to degrade the licensing factor Cdt1 after origins fire, raising the question of how cells prevent re-replication in early S phase. Here, using quantitative microscopy, we show that Cdt1 inhibits DNA synthesis during an overlap period when cells fire origins while Cdt1 is still present. Cdt1 inhibits DNA synthesis by suppressing CMG helicase progression at replication forks through the MCM-binding domain of Cdt1, and DNA synthesis commences once Cdt1 is degraded. Thus, instead of separating licensing from firing to prevent re-replication in early S phase, cells separate licensing from DNA synthesis through Cdt1-mediated inhibition of CMG helicase after firing.

opencc-by-4.0Aug 2021View details →

Modulation of the tumour promoting functions of cancer associated fibroblasts by phosphodiesterase type 5 inhibition increases the efficacy of chemotherapy in human preclinical models of esophageal adenocarcinoma

These are whole-slide digital pathology images of esophageal adenocarcinoma (EAC) patient-derived xenograft models. EAC biopsy specimens were cultured in vitro before implanting into immuno-deficient mice. Mice were then divided into the following treatment groups and treated with combinations of chemotherapy and PDE5 inhibitors as follows: <ol> <li>Untreated</li> <li>Epirubicin + Cisplatin + Capecitabine (ECX)</li> <li>ECX + Vardenafil</li> <li>ECX + Tadalafil</li> </ol> All whole slide images are in Olympus .vsi format and can be opened using the BioFormats library in QuPath. We have also included classifiers and scripts for performing the digital pathology analysis in QuPath, and information to link each mouse with treatment groups and corresponding whole slide images. File metadata: classifiers.zip - Folder containing pixel classifiers used for segmentation of IHC stains. These classifiers are to be important into QuPath for the respective analysis of Periostin (POSTN) and alpha Smooth Muscle Actin (SMA) staining using the included Groovy scripts in the scripts folder. SMA.zip - Folder containing whole slide images of mouse patient-derived xenograft tumours in .vsi format (Olympus VS110). Immunohistochemistry with anti-alpha Smooth Muscle Actin antibody. POSTN.zip - Folder containing whole slide images of mouse patient-derived xenograft tumours in .vsi format (Olympus VS110). Immunohistochemistry with anti-Periostin antibody. scripts.zip - Folder containing all groovy scripts used in QuPath for tissue detection (Whole Section Tissue Detection.groovy), and segmentation of POSTN (POSTN Quantification Mice.groovy) and SMA staining tissue areas (SMA Quantification Mice.groovy). POSTN.xlsx - Data on the mice for which anti-POSTN IHC is available. Column names as follows: Image: file name (corresponding to .vsi file). Mouse_ID: Mouse identifier. Treatment: Treatment administered. ECX - Epirubicin, Cisplatin and Capecitabine. POSTN_Area: Total measured area of POSTN+ tissue in the tissue section (in um^2). Total_Area: Total area of the tissue section (in um^2). percentage_POSTN: Percentage of total tissue area that is stained with POSTN (%). SMA.xlsx - Data on the mice for which anti-SMA IHC is available. Column names as follows: Image: file name (corresponding to .vsi file). Mouse_ID: Mouse identifier. Treatment: Treatment administered. ECX - Epirubicin, Cisplatin and Capecitabine. SMA_Area: Total measured area of SMA+ tissue in the tissue section (in um^2). Total_Area: Total area of the tissue section (in um^2). percentage_SMA: Percentage of total tissue area that is stained with SMA (%).

opencc-by-4.0Apr 2022View details →

Arthrogenic Muscle Inhibition - decomposed EMG

ACL-injured and healthy controls performed isometric contractions (10-50% maximal voluntary contractions) while EMG was being measured of the quadriceps and hamstrings. The EMG was decomposed to individual motor unit characteristics.

opencc-by-4.0Jul 2023View details →

Dataset for "Reactivation strategies for nucleation-inhibited catalyst beds in continuously operated gas-release reactions from liquids."

This dataset contains the measurements that were used to create the diagrams in the manuscript "Reactivation strategies for nucleation-inhibited catalyst beds in continuously operated gas-release reactions from liquids."

opencc-by-4.0Aug 2023View details →

Data from: Single-nucleus RNA-seq and ATAC-seq in outbred rats with divergent cocaine addiction behaviors reveal long-term changes in gene regulation and GABAergic inhibition in the amygdala

This dataset accompanies our publication titled: "Single-nucleus RNA-seq and ATAC-seq in outbred rats with divergent cocaine addiction behaviors reveal long-term changes in gene regulation and GABAergic inhibition in the amygdala." Files Included: 1. geno.N26.vcf.gz     - Description: Contains genotypes for 26 Heterogeneous Stock rats whose gene expression was predicted. 2. pred_expr.Brain.N26.tsv     - Description: This tab-delimited table contains predicted relative gene expression in the brain for 26 Heterogeneous Stock rats.      - Details: Predictions were made for 8,997 genes from linear models based on cis-eQTLs from whole brain hemisphere tissue downloaded from the <a href="https://ratgtex.org/download/">RatGTEx Portal</a>. A gene is included in the table if it had at least one significant cis-eQTL, and if its predicted expression in these 26 animals had nonzero variance. The values in the table give the predicted log2(relative expression), where log2(2) = 1 is the baseline expression from the two haplotypes of the gene if it had only reference alleles at all its regulatory loci.     - Additional Info: Predictions were generated using gene_expr_pred.py available at https://github.com/PejLab/gene_expr_pred An explanation of the prediction model is given in https://doi.org/10.1101/2022.01.28.478116 3. Behavioral data.xlsx     - Description: Contains behavioral data for the Heterogeneous Stock (HS) rats.     - Organization: Each sheet in the file corresponds to data for a specific figure. Additional Dataset Locations: The primary datasets generated during this study can be found on the Gene Expression Omnibus under accession number <a href="https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE212417">GSE212417</a> Publicly Available Datasets Utilized: - Rattus norvegicus Ensembl v98 reference genome and genome assembly: <a href="http://useast.ensembl.org/Rattus_norvegicus/Info/Index">Rnor_6.0 </a> - JASPAR2022 transcription factor binding profiles for vertebrates: <a href="https://jaspar.genereg.net/">JASPAR</a> - ENCODE Honeybadger 2 ChIP-seq: <a href="https://personal.broadinstitute.org/meuleman/reg2map/">Broad Institute</a> - Liu et al. 2019106 GWAS for tobacco and nicotine addiction summary statistics: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6358542/">PubMed</a> - RatGTEx Portal tissue-specific cis-eQTLs: <a href="https://ratgtex.org/download/">RatGTEx Portal </a> - 1000 Genomes European reference panel: <a href="https://alkesgroup.broadinstitute.org/LDSCORE/">Alkes Group</a> - KEGG pathways: <a href="https://www.kegg.jp/kegg/rest/keggapi.html">KEGG API</a>

ClinicalTrials.gov40/100

Study to Demonstrate the Efficacy (Including Inhibition of Structural Damage), Safety and Tolerability up to 2 Years of Secukinumab in Active Psoriatic Arthritis

ClinicalTrials.gov study NCT02404350. IPD Sharing: UNDECIDED. Countries: 28. Publications: 6.

restrictedIPD-UNDECIDEDFeb 2026View details →

publicFeb 2025View details →

Alteration of reproductive behaviors by aromatase inhibition is population-dependent in an African cichlid fish

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publicOct 2024View details →

Data from: Functional PET/MRI reveals active inhibition of neuronal activity during optogenetic activation of the nigrostriatal pathway

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publicNov 2022View details →

Data for: Cdt1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis

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