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219 results for “molecular investigation”
FIGURE 3 in Morphological and molecular systematic investigation of Laurencia karachiana sp. nov. (Ceramiales, Rhodophyta) from Karachi, Pakistan
FIGURE 3. Neighbor-joining (NJ) analysis for COI-5P sequences. Bootstrap values for NJ (2000 replicates) are shown at nodes, values under 70 were not considered. Sequence generated in this study in bold.
FIGURE 2 in Morphological and molecular systematic investigation of Laurencia karachiana sp. nov. (Ceramiales, Rhodophyta) from Karachi, Pakistan
FIGURE 2. Consensus tree derived from Maximum likelihood (ML) analyses of rbcL sequences. Bootstrap supports for NJ (2000 replicates)/ML (1000 replicates)/PP <0.95 are given at the nodes. Sequence generated in this study in bold; – indicates lack of bootstrap support or values under 70; *indicates full support.
FIGURE 1 in Morphological and molecular systematic investigation of Laurencia karachiana sp. nov. (Ceramiales, Rhodophyta) from Karachi, Pakistan
FIGURE 1. Map of Karachi coast showing sampling sites. Site 1. French Beach (Station/Ledge 1), Site 2. French Beach (Station/Ledge 2), Site 3. Hawke's Bay.
Supplementary Information - Chapter 1. Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda-cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)
<p><span>Laboratory-selected resistant strains of <em>Euschistus heros</em> to thiamethoxam (NEO) and lambda-cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in <em>E. heros</em> remain unresolved. We utilized comparative transcriptome profiling and single nucleotide polymorphism (SNP) calling of susceptible and resistant strains of <em>E. heros</em> to investigate the molecular mechanism(s) underlying resistance.</span><span> </span><span>The <em>E. heros</em> transcriptome was assembled, generating 91 673 transcripts with a mean length of 720 bp and N50 of 1795 bp. Comparative gene expression analysis between the susceptible (SUS) and NEO strains identified 215 significantly differentially expressed (DE) transcripts. DE transcripts associated with the xenobiotic metabolism were all up-regulated in the NEO strain. The comparative analysis of the SUS and PYR strains identified 204 DE transcripts, including an esterase (esterase FE4), a glutathione-<em>S</em>-transferase, an ABC transporter (ABCC1) and aquaporins that were up-regulated in the PYR strain. We identified 9588 and 15 043 nonsynonymous SNPs in the PYR and NEO strains. One of the SNPs (D70N) detected in the NEO strain occurs in a subunit (α5) of the nAChRs, the target site of neonicotinoid insecticides. Nevertheless, this residue position in α5 is not conserved among insects.</span><span> </span><span>Neonicotinoid and pyrethroid resistance in laboratory-selected <em>E. heros</em> is associated with a potential metabolic resistance mechanism by the overexpression of proteins commonly involved in the three phases of xenobiotic metabolism. Together these findings provide insight into the potential basis of resistance in <em>E. heros</em> and will inform the development and implementation of resistance management strategies against this important pest.</span></p> <p><strong><span>*Published in: </span></strong><em><span>Pest Management Science</span></em><span><span> 79.12 (2023): 5349-5361</span>. <a href="https://doi.org/10.1002/ps.7745">https://doi.org/10.1002/ps.7745</a></span></p>
Dataset for state-specific reaction of OCS+Ne* from "A novel crossed-molecular-beam experiment for investigating reactions of state- and conformationally selected strong-field-seeking molecules"
<p><strong>Data and analysis for all figures containing experimental data of the paper:</strong></p> <p>•Title: A novel crossed-molecular-beam experiment for investigating reactions of state- and conformationally selected strong-field-seeking molecules</p> <p>•Authors: L. Ploene, P. Straňák, H. Gao, J. Küpper and S.Willitsch</p> <p>•Journal: Molecular Physics</p> <p>DOI: https://doi.org/10.1080/00268976.2021.1965234</p> <p> </p> <p><strong>The PDF contains three flow-chart diagrams, describing how the data has been analysed:</strong></p> <p>•Diagram 1: Deflection profiles of OCS by femtosecond-ionisation compared to MC trajectory simulations for Paper-Figure 3</p> <p>•Diagram 2: Typical TOF-MS trace of Ne* + OCS for Paper-Figure 4 and Experimental deflection profile of OCS+ and S+ from chemi-ionisation reaction Ne* + OCS for Paper-Figure 5</p> <p>•Diagram 3: Least-square fitting of experimental deflection profiles of OCS+and S+ with state-specific simulated deflection profiles for Paper-Figure 6a and 6b</p>
FIGURE 59 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 59. Chronogram showing an estimated phylogeny with divergence times for the Pauropsalta annulata species group, along with outgroups from the tribe Cicadettini, based on CO1 and dynamin data (modelled independently). The topology is a maximum clade credibility from an MCMC search, enforcing a relaxed molecular clock with branch lengths modelled using a GTR + I + G model in *BEAST. Node support is indicated by black closed circles (BPP=1.00) and grey closed circles (BPP=0.95–0.99) from BEAST. Clock calibration is based on a rate of 0.0115s/s/myr for CO1 (see Phylogenetic Analysis Methodology section).
FIGURE 55 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 55. Results of two Non-metric Multidimensional Scaling ordination analyses using the durations of the four song segments (Fig. 54) for Pauropsalta annulata (red), Pauropsalta tremula (purple), Pauropsalta notialis notialis (orange), Pauropsalta notialis incitata (blue) and Pauropsalta notialis notialisxincitata (green) (n=532). Closed points denote individuals recorded in sympatry with other species in the P. annulata species complex, whereas open outlined points are individuals recorded in allopatry. A cluster analysis revealed five clusters among the data, as indicated, and the composition of each is detailed in the text.
FIGURE 52 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 52. Male calling song structure of Pauropsalta ayrensis Ewart illustrated in expanded waveform plots (explained in Fig. 8), showing both buzzing and lilting components. The spectrogram at the bottom of the figure displays song frequency, which exhibits no modulation between the song components in this species. This specimen was recorded in the field at Eidsvold (25°22'S 151°07'E).
FIGURE 51 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 51. Map of eastern Australia showing the geographical distribution of Pauropsalta ayrensis Ewart. Large triangles represent specimen records (see material examined) and small triangles represent aural records (some recorded).
FIGURE 50 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 50. Waveform plots illustrating the lilting component of the male calling song of Pauropsalta rubristrigata (Goding and Froggatt) from four different localities, including: (i) Penola (37°24'S 140°50'E), (ii) Nimmitabel (36°31'S 149°14'E), (iii) Napoleon Reef (33°26'S149°45'E), and (iv) Woods Reserve (35°28'S 148°57'E). Recording (iv) is shown in two fragments from the same individual recording. Mean phrase repetition rates (PRR) for each recording are provided to the right of each plot for reference. The upper fragment shows the typical lilting component and the lower fragment illustrates a transition into the extended lilting component (a grey dashed line indicates the point of transition). Recording (i) was made with RS6 by B. Haywood, while all other recordings were made by LWP using RS5 (see methods).
FIGURE 49 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 49. Male calling song structure of Pauropsalta rubristrigata (Goding and Froggatt) illustrated in expanded waveform plots (explained in Fig. 8), showing both buzzing and lilting components. The spectrogram at the bottom of the figure displays song frequency, which exhibits no modulation between the song components in this species. This specimen was recorded in the field at Nimmitabel (36°31'S 149°14'E) using RS5 (see methods).
FIGURE 56 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 56. Phylogram showing an estimated phylogeny for the Pauropsalta annulata species group, along with outgroups from the tribe Cicadettini, based on CO1 data. The topology is a majority rule consensus tree from an MCMC search, with branch lengths simulated using a GTR + I + G model in MrBayes. Node support values to the left of each node are from a RAxML reconstruction, with Bayesian Posterior Probabilities (BPP) from MrBayes represented by small closed circles (BPP=0.95–0.99) and large closed circles (BPP=1.00) on the relevant nodes.
FIGURE 54 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 54. Oscillogram showing two phrases from the lilting part of the male calling song of Pauropsalta notialis incitata. This is the component to which the female responds during acoustic duets, as shown on the figure. The durations of the various song segments that are demarcated with lines were measured for statistical comparisons, including the long echeme (segment 1), silence between echemes (segment 2), short echeme (segment 3) and silence for female response (segment 4).
FIGURE 47 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 47. Male calling song structure of Pauropsalta inversa sp. nov. illustrated in expanded waveform plots (explained in Fig. 8), showing both buzzing and lilting components. The spectrogram at the bottom of the figure displays song frequency, which exhibits no modulation between the song components in this species. This specimen was recorded in the field at Eidsvold (25°22'S 151°07'E).
FIGURE 45 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 45. Waveform plots illustrating the male calling song of Pauropsalta corymbiae sp. nov. from six different localities, including: (i) Bull's Gully via Adavale (25°58'S 144°59'E), (ii) Mica Creek via Mount Isa (20°49'S 139°27'E), (iii) Boulia (22°55'S 139°55'E), (iv) 48km E. of Middleton (22°16'S 141°56'E), (v) 42 km N. of Barrow Creek (21°54'S 133°34'E), and (vi) Minilya River (23°49'S 114°00'E). Mean phrase repetition rates (PRR) for each recording are provided to the right of each plot for reference. Recordings (iv) and (v) illustrate the transition between the introductory component and the lilting component. The approximate point of this transition is indicated with a grey dashed line. Recordings (i) and (ii) were made with RS3 by A. Ewart, while (iii), (iv) and (vi) were made by K. Hill with RS4, and (v) was made by D. Marshall also using RS4 (see methods).
FIGURE 48 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 48. Waveform plots illustrating the lilting component of the male calling song of Pauropsalta inversa sp. nov. from six different localities, including: (i) Eidsvold (25°22'S 151°07'E), (ii) Mundubbera (25°35'S 151°18'E), (iii) Mount Hope (32°50'S 145°53'E), (iv) Goolgowi (33°59'S 135°43'E), (v) Clermont (22°50'S 147°38'E), and (vi) Mount Moffatt (24°52'S 148°01'E). Mean phrase repetition rates (PRR) for each recording are provided to the right of each plot for reference. Recordings (iii) and (iv) were made with RS5, while the remainder were made with RS1 (see methods).
FIGURE 44 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 44. Male calling song structure of Pauropsalta corymbiae sp. nov. illustrated in expanded waveform plots (explained in Fig. 8), showing both introductory and lilting components. The spectrogram at the bottom of the figure displays song frequency, with arrows indicating side bands. This specimen was recorded with RS3 (see methods) at Bull's Gully, Adavale (25°58'S 144°59'E) by A. Ewart.
FIGURE 43 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 43. Map of mainland Australia showing the geographical distribution of Pauropsalta inversa sp. nov., P. rubristrigata (Goding and Froggatt) and P. corymbiae sp. nov. Large symbols represent specimen records (see type data or material examined), and small symbols represent aural records (some recorded).
FIGURE 41 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 41. Structure of the lilting component of the male calling song of Pauropsalta kobongoides sp. nov. illustrated in expanded waveform plots (explained in Fig. 8). The spectrogram at the bottom of the figure displays song frequency for the lilting component. This recording is a continuation of the one illustrated in Figure 40 from Weengallon (28°22'S 149°03'E).
FIGURE 40 in <p class="HeadingRunIn" align="left"><strong>A revision of the <em>Pauropsalta annulata </em>Goding & Froggatt species group (Hemiptera: Cicadidae) based on morphology, calling songs and ecology, with investigations into calling song structure, molecular phylogenetic relationships and a case of hybridisation between two subspecies</strong></p>
FIGURE 40. Structure of the buzzing component of the male calling song of Pauropsalta kobongoides sp. nov. illustrated in expanded waveform plots (explained in Fig. 8). The spectrograms at the bottom of the figure display song frequency, for modulates between different parts of the buzzing component. This specimen was recorded in the field at Weengallon (28°22'S 149°03'E).
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.