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105 results for “molecular sexing”
Data from: Enhanced understanding of predator–prey relationships using molecular methods to identify predator species, individual and sex
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Genome-wide SNP analysis of Siamese cobra (Naja kaouthia) reveals the molecular basis of transitions between Z and W sex chromosomes and supports the presence of an ancestral super-sex chromosome in amniotes
<p>Elucidation of the process of sex chromosome differentiation is necessary to understand the dynamics of evolutionary mechanisms in organisms. The Siamese cobra (<i>Naja kaouthia</i>) exhibits ZZ/ZW heteromorphic sex chromosomes. The W sex chromosome contains a large number of repeats and shares several amniote sex chromosomal linkages. In conjunction with recent advances in high-throughput sequencing, Diversity Arrays Technology (DArTseq™) provides an effective approach to identify sex-specific loci that are epoch-making, to understand the dynamics of molecular transitions between the Z and W sex chromosomes in a snake lineage. From a total of 543 perfectly sex-linked loci, 90 loci showed partial homology with several amniote sex chromosomal linkages, and 89 loci were homologous to transposable elements, which suggests that recombination suppression may be the crucial step in snake sex chromosome differentiation. Two loci were confirmed as W-specific nucleotides in females but not in males in the population examined by PCR amplification; one of the two loci (locus id: 100002617) was further amplified in females of the Indochinese spitting cobra (<i>N. siamensis</i>) but not in the other 22 snake species examined. Female-specific DArT markers were identified in Siamese cobra. These loci might result from a sex chromosome differentiation process between Z and W and involve putative sex-determination regions in Siamese cobra. Short sequences derived from DArTseq™ technology also shared linkage homologies among amniote sex chromosomes, which supports the hypothesis of an ancestral super-sex chromosome with overlaps of partial sex chromosomal linkages. The locus (id: 100002617) shared in <i>N. kaouthia</i> and <i>N. siamensis</i>, but among 22 other snake species, indicates inheritance from a common ancestor as synapomorphic loci in the <i>Naja</i> lineage. The ease of use of the DArT markers and DArTseq™ platform provides a useful strategy for future research on sex chromosome evolution in snakes.</p>
Data from: A universal and reliable assay for molecular sex identification of three-spined sticklebacks (Gasterosteus aculeatus)
In heterogametic species, biological differences between the two sexes are ubiquitous, and hence, errors in sex identification can be a significant source of noise and bias in studies where sex-related sources of variation are of interest or need to be controlled for. We developed and validated a universal multi-marker assay for reliable sex identification of three-spined sticklebacks (Gasterosteus aculeatus). The assay makes use of genotype scores from three sex-linked loci, and utilizes Bayesian probabilistic inference to identify sex of the genotyped individuals. The results, validated with 286 phenotypically sexed individuals from six populations of sticklebacks representing all major genetic lineages (cf. Pacific, Atlantic and Japan Sea) indicate that in contrast to commonly used single-marker based sex identification assays, the developed multi-marker assay should be 100% accurate. Since the markers in the assay can be scored from agarose gels, it provides a quick and cost-efficient tool for universal sex identification of three-spined sticklebacks. The general principle of combining information from multiple markers to improve the reliability of sex identification is transferable and can be utilized to develop and validate similar assays for other species.
FIGURE 10. Miobantia nordestina n in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 10. Miobantia nordestina n. sp., dorsal habitus. A, male holotype; B, female allotype.
FIGURE 8. Miobantia arctissima n in A revision of Miobantia Giglio-Tos, 1917 (Mantodea: Thespidae, Miobantiinae), with molecular association of dimorphic sexes and immature stages
FIGURE 8. Miobantia arctissima n. sp., dorsal habitus, male holotype.
Fig. 1. A in Intersexual Size And Plumage Differences In Tree Sparrows (Passer Montanus) - A Morphological Study Based On Molecular Sex Determination
Fig. 1. A photography of the badge used for measurements; hatched area shows the badge area as measured with the image analyzing software (see text)
Figure 1 from: Medeiros RT, Chaves FG, Vecchi MB, Nogueira DM, Alves MAS (2019) Molecular sexing and intersexual differences in the morphometry of the Hangnest Tody-Tyrant Hemitriccus nidipendulus (Passeriformes: Rhynchocyclidae). Zoologia 36: 1-6. https://doi.org/10.3897/zoologia.36.e32771
Figure 1 The DNA fragments of the intronic region of the CHDZ/CHDW alleles of the Hangnest Tody-Tyrant individuals captured in the Costa do Sol State Park, in southeastern Brazil. The fragments were amplified by Polymerase Chain Reaction (PCR) and electrophoresed in 2.5% agarose gel. The first column on the left (L) is a 50 bp DNA ladder, followed by the CHDZ/CHDW amplicons of the females, which present two bands (CHDZ = ~360 bp and CHDW = 400 bp), while males had only one band (CHDZ), with the negative control (N), consisting of only water.
Figure 1 from: Kurina O, Õunap E, Ramel G (2011) Baeopterogyna mihalyii Matile (Diptera, Mycetophilidae): association of sexes using morphological and molecular approaches with the first description of females. ZooKeys 114: 15-27. https://doi.org/10.3897/zookeys.114.1364
Figure 1 - Bayesian phylogenetic tree (GTR+I model) of selected Mycetophilidae taxa, based on a 762 bp fragment of a COI gene. Bayesian posterior probabilities are given above the branches; bootstrap support for the ML/NJ/MP trees, which exhibited identical topology, are presented below the branches.
Figure 3 from: Kurina O, Õunap E, Ramel G (2011) Baeopterogyna mihalyii Matile (Diptera, Mycetophilidae): association of sexes using morphological and molecular approaches with the first description of females. ZooKeys 114: 15-27. https://doi.org/10.3897/zookeys.114.1364
Figure 3 - Baeopterogyna mihalyii. a female terminalia, lateral view b female terminalia, dorsal view c female terminalia, ventral view d male terminalia, ventral view. Scale = 0.2 mm.<br> cerc = cercus; gc = gonocoxite; gp = gonapophysis; gst = gonostylus; hyp = hypoproct; st = sternite; tg = tergite
Figure 2 from: Kurina O, Õunap E, Ramel G (2011) Baeopterogyna mihalyii Matile (Diptera, Mycetophilidae): association of sexes using morphological and molecular approaches with the first description of females. ZooKeys 114: 15-27. https://doi.org/10.3897/zookeys.114.1364
Figure 2 - Baeopterogyna mihalyii. a female b male c female wing d female antenna e female thorax f female head (last palpal segments absent). Scale = 1 mm (a, b, c), 0.2 mm (d, e, f).<br> anepm = anepimeron; anepst = anepisternum; aprnt = antepronotum; htl = halter; ltg = laterotergite; mtepst = metepisternum; mtg = mediotergite; proepst = proepisternum; preepst = preepisternum; sc = scutum.
Data from: Molecular genetics of sex identification, breed ancestry and polydactyly in the Norwegian Lundehund breed
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Data from: A universal and reliable assay for molecular sex identification of three-spined sticklebacks (Gasterosteus aculeatus)
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Genome-wide SNP analysis of Siamese cobra (Naja kaouthia) reveals the molecular basis of transitions between Z and W sex chromosomes and supports the presence of an ancestral super-sex chromosome in amniotes
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Data from: Identification of sex-specific molecular markers using restriction site associated DNA sequencing (RAD-seq)
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The complete molecular atlas of adult C. elegans glia across sexes
GEO Series GSE256266. Caenorhabditis elegans. 4 samples. Type: Expression profiling by high throughput sequencing.
Sex-specific molecular signature of mouse podocytes in homeostasis and in response to pharmacological challenge with rapamycin
GEO Series GSE276967. Mus musculus. 20 samples. Type: Expression profiling by high throughput sequencing.
Reproduction-associated and sex determining genes expressed differently in testes of genotypic and temperature-induced males of gibel carp (Carassius gibelio): the molecular mechanisms underlying the
GEO Series GSE285878. Carassius gibelio. 26 samples. Type: Expression profiling by high throughput sequencing.
Molecular and genetic characterization of sex-linked orange coat color in the domestic cat [C4F1]
GEO Series GSE290419. Felis catus. 1 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome of tambaqui Colossoma macropomum during gonad differentiation: different molecular signals leading to sex identity
GEO Series GSE130895. Colossoma macropomum. 10 samples. Type: Expression profiling by high throughput sequencing.
Postnatal persistence of sex-dependent renal developmental programmed structural and molecular changes in nonhuman primates
GEO Series GSE149895. Homo sapiens; Papio hamadryas. 24 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.