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Supplementary material 7 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Haplotype network of the COI data analyzed of D. pampeana
Supplementary material 2 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Scree plots obtained from the morphometric and meristic data analyzed
Supplementary material 5 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Tukey box plot of most distinctive meristic data observed in analyzed specimens of Diapoma pampeana
Figure 4 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Figure 4 Most discriminant axes obtained from the PCA analyses performed using morphometric and meristic data of studied specimens of Diapoma pampeana (in each plot, the loadings are scaled to 90% of the PC scores). Size-corrected measurements: A. PC1 vs. PC2 plot; B. PC3 vs. PC4 plot. Meristic data; C. PC1 vs. PC2 plot; D. PC3 vs. PC4 plot. Only these variables that most loaded the components are indicated as follows: E- depth at dorsal-fin origin; F- snout to dorsal-fin origin; G- snout to pelvic-fin origin; H- Snout to anal-fin origin; I- distance between dorsal- and adipose-fin origins; J- dorsal fin to caudal-fin base; K- anal-fin base length; L- caudal peduncle length; M- longitudinal scales; N- lateral-line scales; P- scales between lateral line-dorsal origin; Q- scales between lateral line-pelvic origin; R- circumpeduncular scales; S- predorsal scales; T- number of branched anal-fin rays; U- gill rakers on upper limb of branchial arch; V- gill rakers on lower limb of branchial arch; W- number of maxillary teeth; X- number of dentary teeth.
Figure 3 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Figure 3 Geographic distribution of Diapoma pampeana. A. View within South America; B. View within Brazil and Uruguay. All studied specimens from the Pando (Circle), Santa Lucía (triangle), Yi (diamond), and Upper Negro (star; holotype represented by not-filled pattern) are depicted. Other records presented by Ito et al. (2022) are also included.
Figure 1 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Figure 1 Coloration in life of D. pampeana (A, B) from the Pando stream, Canelones Uruguay. Photo by J. Pfleiderer.
Figure 6 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Figure 6 Bayesian topology of phylogenetic relationships among the analyzed Diapoma species (comparing specimens of D. pampeana from the Pando stream and Upper Negro basin) based on COI sequence data. Numbers at nodes correspond to posterior probabilities.
Supplementary material 6 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Uncorrected pairwise genetic distances using the COI data matrix
Supplementary material 3 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Total variance accounted for the PCA performed for the morphometric and meristic data
Supplementary material 1 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
All COI sequences analyzed in the present work
Figure 2 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Figure 2 Extern morphology of studied specimens of Diapoma pampeana. A. MLP 11443, male, 30.6 mm SL, Uruguay, Pando Stream; B. MLP 11445, female, 35.1 mm SL, Uruguay, Pando Stream; C. MHNM 1125, female, 31.3 mm SL, Uruguay, Canelón Grande Stream; D. MHNM 4018, male, 29.8 mm SL, Uruguay, marginal lagoon to Rio Yi; E. MHNM 4018, female, 29.3 mm SL, Uruguay, marginal lagoon to Rio Yi. Photographs of the specimens from the Upper Negro are available in Ito et al. (2022). Scale bar: 1 mm.
Figure 5 from: Vanegas-Ríos JA, Serra Alanís WS, Azpelicueta MM, Litz T, Malabarba LR (2024) Population variation of Diapoma pampeana (Characiformes, Characidae, Stevardiinae) from an isolated coastal drainage in Uruguay, with new records: comparing morphological and molecular data. Zoosystematics and Evolution 100(1): 69-85. https://doi.org/10.3897/zse.100.112778
Figure 5 Neighbor-Joining topology of analyzed Diapoma specimens based on Tamura-Nei model and COI sequence data. Bootstrap values (10000 replicates) are shown below the branches. SBL = 0.783.
Supplementary material 2 from: Lessi BF, Geneletti D, Cortinovis C, Dias MM, Reis MG (2024) Bird richness and Ecosystems Services across an urban to natural gradient in south-eastern Brazil: implications for landscape planning and future scenarios. One Ecosystem 9: e114955. https://doi.org/10.3897/oneeco.9.e114955
Supplementary material 2
Supplementary material 3 from: Lessi BF, Geneletti D, Cortinovis C, Dias MM, Reis MG (2024) Bird richness and Ecosystems Services across an urban to natural gradient in south-eastern Brazil: implications for landscape planning and future scenarios. One Ecosystem 9: e114955. https://doi.org/10.3897/oneeco.9.e114955
Supplementary material 3
Supplementary material 1 from: Lessi BF, Geneletti D, Cortinovis C, Dias MM, Reis MG (2024) Bird richness and Ecosystems Services across an urban to natural gradient in south-eastern Brazil: implications for landscape planning and future scenarios. One Ecosystem 9: e114955. https://doi.org/10.3897/oneeco.9.e114955
Supplementary material 1
Figure 2 from: Issa R, Abu Samak M, Abbas MM, Al-Qaisi T, Obeidat R, Ghanim B, Qinna N (2024) Effect of Equisetum ramosissimum Desf. on body weight and Leptin/Ghrelin in standard and high-fat diet. Pharmacia 71: 1-7. https://doi.org/10.3897/pharmacia.71.e117823
Figure 2 Percent Body Weight change in healthy and obese animals treated with E. ramosissimum extract. Comparison of weight change was made between: Standard Diet (SD); SD receiving Plant extract (SDP); High-fat Diet (HD); and HD receiving Plant extract (HDP). ***: p<0.001 in comparison to SD, as calculated through t-test.
Figure 3 from: Issa R, Abu Samak M, Abbas MM, Al-Qaisi T, Obeidat R, Ghanim B, Qinna N (2024) Effect of Equisetum ramosissimum Desf. on body weight and Leptin/Ghrelin in standard and high-fat diet. Pharmacia 71: 1-7. https://doi.org/10.3897/pharmacia.71.e117823
Figure 3 Serum concentrations of leptin and ghrelin and their corresponding ratios. Serum concentrations of leptin and ghrelin and their corresponding ratios were quantified for animals receiving Standard Diet (SD); SD receiving Plant extract (SDP); High-fat Diet (HD); and HD receiving Plant extract (HDP). Ratios of leptin/ghrelin were calculated and presented in normalized values to the standard body levels (right y-axis), specifically per animals receiving SD. **: p<0.01; *p<0.05 in comparison to SD.
Figure 1 from: Issa R, Abu Samak M, Abbas MM, Al-Qaisi T, Obeidat R, Ghanim B, Qinna N (2024) Effect of Equisetum ramosissimum Desf. on body weight and Leptin/Ghrelin in standard and high-fat diet. Pharmacia 71: 1-7. https://doi.org/10.3897/pharmacia.71.e117823
Figure 1 Total Phenol and Flavonoid content in E. ramosissimum extract. Extracts are quantified against equivalent quantities of reference standards, specifically Gallic acid and quercetin, respectively.
MM - Frailty
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iEPI - 0.7mm - 3shot
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